LOCUS       RG235056                9362 bp    DNA     circular  SYN 
DEFINITION  DGKZ (tGFP-tagged) - Homo sapiens diacylglycerol kinase, zeta (DGKZ), transcript variant 5
ACCESSION   NM_001199266
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3843..4547 
                     /label="GFP"  
     misc_feature    4594..5179 
                     /label="PolyA signal"  
     misc_feature    7271..7916 
                     /label="ColE1"  
     misc_feature    5633..6427 
                     /label="Neo"  
     misc_feature    5264..5566 
                     /label="SV40 ori"  
     misc_feature    7931..8791 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3822..3828 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3822 
                     /label="DGKZ"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCCGCGG GACGGTAGCC CCGAGGCCCG GAGCAGCGAC TCCGAGTCGG 
     1081 CTTCCGCCTC GTCCAGCGGC TCCGAGCGCG ACGCCGGTCC CGAGCCGGAC AAGGCGCCGC 
     1141 GGCGACTCAA CAAGCGGCGC TTCCCGGGGC TGCGGCTCTT CGGGCACAGG AAAGCCATCA 
     1201 CCAAGTCGGG CCTCCAGCAC CTGGCCCCCC CTCCGCCCAC CCCTGGGGCC CCGTGCAGCG 
     1261 AGTCAGAGCG GCAGATCCGG AGTACAGTGG ACTGGAGCGA GTCAGCGACA TATGGGGAGC 
     1321 ACATCTGGTT CGAGACCAAC GTGTCCGGGG ACTTCTGCTA CGTTGGGGAG CAGTACTGTG 
     1381 TAGCCAGGAT GCTGCAGAAG TCAGTGTCTC GAAGAAAGTG CGCAGCCTGC AAGATTGTGG 
     1441 TGCACACGCC CTGCATCGAG CAGCTGGAGA AGATAAATTT CCGCTGTAAG CCGTCCTTCC 
     1501 GTGAATCAGG CTCCAGGAAT GTCCGCGAGC CAACCTTTGT ACGGCACCAC TGGGTACACA 
     1561 GACGACGCCA GGACGGCAAG TGTCGGCACT GTGGGAAGGG ATTCCAGCAG AAGTTCACCT 
     1621 TCCACAGCAA GGAGATTGTG GCCATCAGCT GCTCGTGGTG CAAGCAGGCA TACCACAGCA 
     1681 AGGTGTCCTG CTTCATGCTG CAGCAGATCG AGGAGCCGTG CTCGCTGGGG GTCCACGCAG 
     1741 CCGTGGTCAT CCCGCCCACC TGGATCCTCC GCGCCCGGAG GCCCCAGAAT ACTCTGAAAG 
     1801 CAAGCAAGAA GAAGAAGAGG GCATCCTTCA AGAGGAAGTC CAGCAAGAAA GGGCCTGAGG 
     1861 AGGGCCGCTG GAGACCCTTC ATCATCAGGC CCACCCCCTC CCCGCTCATG AAGCCCCTGC 
     1921 TGGTGTTTGT GAACCCCAAG AGTGGGGGCA ACCAGGGTGC AAAGATCATC CAGTCTTTCC 
     1981 TCTGGTATCT CAATCCCCGA CAAGTCTTCG ACCTGAGCCA GGGAGGGCCC AAGGAGGCGC 
     2041 TGGAGATGTA CCGCAAAGTG CACAACCTGC GGATCCTGGC GTGCGGGGGC GACGGCACGG 
     2101 TGGGCTGGAT CCTCTCCACC CTGGACCAGC TACGCCTGAA GCCGCCACCC CCTGTTGCCA 
     2161 TCCTGCCCCT GGGTACTGGC AACGACTTGG CCCGAACCCT CAACTGGGGT GGGGGCTACA 
     2221 CAGATGAGCC TGTGTCCAAG ATCCTCTCCC ACGTGGAGGA GGGGAACGTG GTACAGCTGG 
     2281 ACCGCTGGGA CCTCCACGCT GAGCCCAACC CCGAGGCAGG GCCTGAGGAC CGAGATGAAG 
     2341 GCGCCACCGA CCGGTTGCCC CTGGATGTCT TCAACAACTA CTTCAGCCTG GGCTTTGACG 
     2401 CCCACGTCAC CCTGGAGTTC CACGAGTCTC GAGAGGCCAA CCCAGAGAAA TTCAACAGCC 
     2461 GCTTTCGGAA TAAGATGTTC TACGCCGGGA CAGCTTTCTC TGACTTCCTG ATGGGCAGCT 
     2521 CCAAGGACCT GGCCAAGCAC ATCCGAGTGG TGTGTGATGG AATGGACTTG ACTCCCAAGA 
     2581 TCCAGGACCT GAAACCCCAG TGTGTTGTTT TCCTGAACAT CCCCAGGTAC TGTGCGGGCA 
     2641 CCATGCCCTG GGGCCACCCT GGGGAGCACC ACGACTTTGA GCCCCAGCGG CATGACGACG 
     2701 GCTACCTCGA GGTCATTGGC TTCACCATGA CGTCGTTGGC CGCGCTGCAG GTGGGCGGAC 
     2761 ACGGCGAGCG GCTGACGCAG TGTCGCGAGG TGGTGCTCAC CACATCCAAG GCCATCCCGG 
     2821 TGCAGGTGGA TGGCGAGCCC TGCAAGCTTG CAGCCTCACG CATCCGCATC GCCCTGCGCA 
     2881 ACCAGGCCAC CATGGTGCAG AAGGCCAAGC GGCGGAGCGC CGCCCCCCTG CACAGCGACC 
     2941 AGCAGCCGGT GCCAGAGCAG TTGCGCATCC AGGTGAGTCG CGTCAGCATG CACGACTATG 
     3001 AGGCCCTGCA CTACGACAAG GAGCAGCTCA AGGAGGCCTC TGTGCCGCTG GGCACTGTGG 
     3061 TGGTCCCAGG AGACAGTGAC CTAGAGCTCT GCCGTGCCCA CATTGAGAGA CTCCAGCAGG 
     3121 AGCCCGATGG TGCTGGAGCC AAGTCCCCGA CATGCCAGAA ACTGTCCCCC AAGTGGTGCT 
     3181 TCCTGGACGC CACCACTGCC AGCCGCTTCT ACAGGATCGA CCGAGCCCAG GAGCACCTCA 
     3241 ACTATGTGAC TGAGATCGCA CAGGATGAGA TTTATATCCT GGACCCTGAG CTGCTGGGGG 
     3301 CATCGGCCCG GCCTGACCTC CCAACCCCCA CTTCCCCTCT CCCCACCTCA CCCTGCTCAC 
     3361 CCACGCCCCG GTCACTGCAA GGGGATGCTG CACCCCCTCA AGGTGAAGAG CTGATTGAGG 
     3421 CTGCCAAGAG GAACGACTTC TGTAAGCTCC AGGAGCTGCA CCGAGCTGGG GGCGACCTCA 
     3481 TGCACCGAGA CGAGCAGAGT CGCACGCTCC TGCACCACGC AGTCAGCACT GGCAGCAAGG 
     3541 ATGTGGTCCG CTACCTGCTG GACCACGCCC CCCCAGAGAT CCTTGATGCG GTGGAGGAAA 
     3601 ACCCCTGCTC CCCCAGCGGG GAGACCTGTT TGCACCAAGC AGCGGCCCTG GGCCAGCGCA 
     3661 CCATCTGCCA CTACATCGTG GAGGCCGGGG CCTCGCTCAT GAAGACAGAC CAGCAGGGCG 
     3721 ACACTCCCCG GCAGCGGGCT GAGAAGGCTC AGGACACCGA GCTGGCCGCC TACCTGGAGA 
     3781 ACCGGCAGCA CTACCAGATG ATCCAGCGGG AGGACCAGGA GACGGCTGTG ACGCGTACGC 
     3841 GGCCGCTCGA GATGGAGAGC GACGAGAGCG GCCTGCCCGC CATGGAGATC GAGTGCCGCA 
     3901 TCACCGGCAC CCTGAACGGC GTGGAGTTCG AGCTGGTGGG CGGCGGAGAG GGCACCCCCG 
     3961 AGCAGGGCCG CATGACCAAC AAGATGAAGA GCACCAAAGG CGCCCTGACC TTCAGCCCCT 
     4021 ACCTGCTGAG CCACGTGATG GGCTACGGCT TCTACCACTT CGGCACCTAC CCCAGCGGCT 
     4081 ACGAGAACCC CTTCCTGCAC GCCATCAACA ACGGCGGCTA CACCAACACC CGCATCGAGA 
     4141 AGTACGAGGA CGGCGGCGTG CTGCACGTGA GCTTCAGCTA CCGCTACGAG GCCGGCCGCG 
     4201 TGATCGGCGA CTTCAAGGTG ATGGGCACCG GCTTCCCCGA GGACAGCGTG ATCTTCACCG 
     4261 ACAAGATCAT CCGCAGCAAC GCCACCGTGG AGCACCTGCA CCCCATGGGC GATAACGATC 
     4321 TGGATGGCAG CTTCACCCGC ACCTTCAGCC TGCGCGACGG CGGCTACTAC AGCTCCGTGG 
     4381 TGGACAGCCA CATGCACTTC AAGAGCGCCA TCCACCCCAG CATCCTGCAG AACGGGGGCC 
     4441 CCATGTTCGC CTTCCGCCGC GTGGAGGAGG ATCACAGCAA CACCGAGCTG GGCATCGTGG 
     4501 AGTACCAGCA CGCCTTCAAG ACCCCGGATG CAGATGCCGG TGAAGAAAGA GTTTAAACGG 
     4561 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4621 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4681 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4741 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4801 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4861 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4921 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4981 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5041 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5101 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5161 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5221 GGTACGCGGC CAGCTTGGCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC 
     5281 TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA 
     5341 AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA 
     5401 ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT 
     5461 TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCGGCC 
     5521 TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG 
     5581 CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT 
     5641 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA 
     5701 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG 
     5761 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA 
     5821 CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT 
     5881 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG 
     5941 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA 
     6001 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT 
     6061 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC 
     6121 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC 
     6181 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA 
     6241 AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCCGA CCGCTATCAG 
     6301 GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC 
     6361 TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT 
     6421 CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGAAAT GACCGACCAA GCGACGCCCA 
     6481 ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA 
     6541 TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG CTGGAGTTCT 
     6601 TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA 
     6661 CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA 
     6721 TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG GCGTAATCAT 
     6781 GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG 
     6841 CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG 
     6901 CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA 
     6961 TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA 
     7021 CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG 
     7081 TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC 
     7141 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC 
     7201 CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC 
     7261 TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC 
     7321 TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA 
     7381 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC 
     7441 ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA 
     7501 ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG 
     7561 CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA 
     7621 GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG 
     7681 GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TTTTTTTGTT TGCAAGCAGC 
     7741 AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG 
     7801 ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA 
     7861 TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG 
     7921 AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT 
     7981 GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG 
     8041 AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC 
     8101 CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA 
     8161 CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC 
     8221 CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT 
     8281 CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC 
     8341 CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT 
     8401 TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC 
     8461 CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT 
     8521 GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA 
     8581 GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA 
     8641 TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG 
     8701 CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA 
     8761 AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT 
     8821 ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA 
     8881 AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT 
     8941 GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG 
     9001 CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG 
     9061 GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC 
     9121 GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT 
     9181 GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT 
     9241 TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT 
     9301 TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT 
     9361 TT
//