LOCUS       RG235042                9344 bp    DNA     circular  SYN 
DEFINITION  DGKZ (tGFP-tagged) - Homo sapiens diacylglycerol kinase, zeta (DGKZ), transcript variant 6
ACCESSION   NM_001199267
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3825..4529 
                     /label="GFP"  
     misc_feature    4576..5161 
                     /label="PolyA signal"  
     misc_feature    7253..7898 
                     /label="ColE1"  
     misc_feature    5615..6409 
                     /label="Neo"  
     misc_feature    5246..5548 
                     /label="SV40 ori"  
     misc_feature    7913..8773 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3804..3810 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3804 
                     /label="DGKZ"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCCGCGG GACGGTAGCC CCGAGGCCCG GAGCAGCGAC TCCGAGTCGG 
     1081 CTTCCGCCTC GTCCAGCGGC TCCGAGCGCG ACGCCGGTCC CGAGCCGGAC AAGGCGCCGC 
     1141 GGCGACTCAA CAAGCGGCGC TTCCCGGGGC TGCGGCTCTT CGGGCACAGG AAAGCCATCA 
     1201 CCAAGTCGGG CCTCCAGCAC CTGGCCCCCC CTCCGCCCAC CCCTGGGGCC CCGTGCAGCG 
     1261 AGTCAGAGCG GCAGATCCGG AGTACAGTGG ACTGGAGCGA GTCAGCGACA TATGGGGAGC 
     1321 ACATCTGGTT CGAGACCAAC GTGTCCGGGG ACTTCTGCTA CGTTGGGGAG CAGTACTGTG 
     1381 TAGCCAGGAT GCTGAAGTCA GTGTCTCGAA GAAAGTGCGC AGCCTGCAAG ATTGTGGTGC 
     1441 ACACGCCCTG CATCGAGCAG CTGGAGAAGA TAAATTTCCG CTGTAAGCCG TCCTTCCGTG 
     1501 AATCAGGCTC CAGGAATGTC CGCGAGCCAA CCTTTGTACG GCACCACTGG GTACACAGAC 
     1561 GACGCCAGGA CGGCAAGTGT CGGCACTGTG GGAAGGGATT CCAGCAGAAG TTCACCTTCC 
     1621 ACAGCAAGGA GATTGTGGCC ATCAGCTGCT CGTGGTGCAA GCAGGCATAC CACAGCAAGG 
     1681 TGTCCTGCTT CATGCTGCAG CAGATCGAGG AGCCGTGCTC GCTGGGGGTC CACGCAGCCG 
     1741 TGGTCATCCC GCCCACCTGG ATCCTCCGCG CCCGGAGGCC CCAGAATACT CTGAAAGCAA 
     1801 GCAAGAAGAA GAAGAGGGCA TCCTTCAAGA GGAAGTCCAG CAAGAAAGGG CCTGAGGAGG 
     1861 GCCGCTGGAG ACCCTTCATC ATCAGGCCCA CCCCCTCCCC GCTCATGAAG CCCCTGCTGG 
     1921 TGTTTGTGAA CCCCAAGAGT GGGGGCAACC AGGGTGCAAA GATCATCCAG TCTTTCCTCT 
     1981 GGTATCTCAA TCCCCGACAA GTCTTCGACC TGAGCCAGGG AGGGCCCAAG GAGGCGCTGG 
     2041 AGATGTACCG CAAAGTGCAC AACCTGCGGA TCCTGGCGTG CGGGGGCGAC GGCACGGTGG 
     2101 GCTGGATCCT CTCCACCCTG GACCAGCTAC GCCTGAAGCC GCCACCCCCT GTTGCCATCC 
     2161 TGCCCCTGGG TACTGGCAAC GACTTGGCCC GAACCCTCAA CTGGGGTGGG GGCTACACAG 
     2221 ATGAGCCTGT GTCCAAGATC CTCTCCCACG TGGAGGAGGG GAACGTGGTA CAGCTGGACC 
     2281 GCTGGGACCT CCACGCTGAG CCCAACCCCG AGGCAGGGCC TGAGGACCGA GATGAAGGCG 
     2341 CCACCGACCG GTTGCCCCTG GATGTCTTCA ACAACTACTT CAGCCTGGGC TTTGACGCCC 
     2401 ACGTCACCCT GGAGTTCCAC GAGTCTCGAG AGGCCAACCC AGAGAAATTC AACAGCCGCT 
     2461 TTCGGAATAA GATGTTCTAC GCCGGGACAG CTTTCTCTGA CTTCCTGATG GGCAGCTCCA 
     2521 AGGACCTGGC CAAGCACATC CGAGTGGTGT GTGATGGAAT GGACTTGACT CCCAAGATCC 
     2581 AGGACCTGAA ACCCCAGTGT GTTGTTTTCC TGAACATCCC CAGGTACTGT GCGGGCACCA 
     2641 TGCCCTGGGG CCACCCTGGG GAGCACCACG ACTTTGAGCC CCAGCGGCAT GACGACGGCT 
     2701 ACCTCGAGGT CATTGGCTTC ACCATGACGT CGTTGGCCGC GCTGCAGGTG GGCGGACACG 
     2761 GCGAGCGGCT GACGCAGTGT CGCGAGGTGG TGCTCACCAC ATCCAAGGCC ATCCCGGTGC 
     2821 AGGTGGATGG CGAGCCCTGC AAGCTTGCAG CCTCACGCAT CCGCATCGCC CTGCGCAACC 
     2881 AGGCCACCAT GGTGCAGAAG GCCAAGCGGC GGAGCGCCGC CCCCCTGCAC AGCGACCAGC 
     2941 AGCCGGTGCC AGAGCAGTTG CGCATCCAGG TGAGTCGCGT CAGCATGCAC GACTATGAGG 
     3001 CCCTGCACTA CGACAAGGAG CAGCTCAAGG AGGCCTCTGT GCCGCTGGGC ACTGTGGTGG 
     3061 TCCCAGGAGA CAGTGACCTA GAGCTCTGCC GTGCCCACAT TGAGAGACTC CAGCAGGAGC 
     3121 CCGATGGTGC TGGAGCCAAG TCCCCGACAT GCCAGAAACT GTCCCCCAAG TGGTGCTTCC 
     3181 TGGACGCCAC CACTGCCAGC CGCTTCTACA GGATCGACCG AGCCCAGGAG CACCTCAACT 
     3241 ATGTGACTGA GATCGCACAG GATGAGATTT ATATCCTGGA CCCTGAGCTG CTGGGGGCAT 
     3301 CGGCCCGGCC TGACCTCCCA ACCCCCACTT CCCCTCTCCC CACCTCACCC TGCTCACCCA 
     3361 CGCCCCGGTC ACTGCAAGGG GATGCTGCAC CCCCTCAAGG TGAAGAGCTG ATTGAGGCTG 
     3421 CCAAGAGGAA CGACTTCTGT AAGCTCCAGG AGCTGCACCG AGCTGGGGGC GACCTCATGC 
     3481 ACCGAGACGA GCAGAGTCGC ACGCTCCTGC ACCACGCAGT CAGCACTGGC AGCAAGGATG 
     3541 TGGTCCGCTA CCTGCTGGAC CACGCCCCCC CAGAGATCCT TGATGCGGTG GAGGAAAACG 
     3601 GGGAGACCTG TTTGCACCAA GCAGCGGCCC TGGGCCAGCG CACCATCTGC CACTACATCG 
     3661 TGGAGGCCGG GGCCTCGCTC ATGAAGACAG ACCAGCAGGG CGACACTCCC CGGCAGCGGG 
     3721 CTGAGAAGGC TCAGGACACC GAGCTGGCCG CCTACCTGGA GAACCGGCAG CACTACCAGA 
     3781 TGATCCAGCG GGAGGACCAG GAGACGGCTG TGACGCGTAC GCGGCCGCTC GAGATGGAGA 
     3841 GCGACGAGAG CGGCCTGCCC GCCATGGAGA TCGAGTGCCG CATCACCGGC ACCCTGAACG 
     3901 GCGTGGAGTT CGAGCTGGTG GGCGGCGGAG AGGGCACCCC CGAGCAGGGC CGCATGACCA 
     3961 ACAAGATGAA GAGCACCAAA GGCGCCCTGA CCTTCAGCCC CTACCTGCTG AGCCACGTGA 
     4021 TGGGCTACGG CTTCTACCAC TTCGGCACCT ACCCCAGCGG CTACGAGAAC CCCTTCCTGC 
     4081 ACGCCATCAA CAACGGCGGC TACACCAACA CCCGCATCGA GAAGTACGAG GACGGCGGCG 
     4141 TGCTGCACGT GAGCTTCAGC TACCGCTACG AGGCCGGCCG CGTGATCGGC GACTTCAAGG 
     4201 TGATGGGCAC CGGCTTCCCC GAGGACAGCG TGATCTTCAC CGACAAGATC ATCCGCAGCA 
     4261 ACGCCACCGT GGAGCACCTG CACCCCATGG GCGATAACGA TCTGGATGGC AGCTTCACCC 
     4321 GCACCTTCAG CCTGCGCGAC GGCGGCTACT ACAGCTCCGT GGTGGACAGC CACATGCACT 
     4381 TCAAGAGCGC CATCCACCCC AGCATCCTGC AGAACGGGGG CCCCATGTTC GCCTTCCGCC 
     4441 GCGTGGAGGA GGATCACAGC AACACCGAGC TGGGCATCGT GGAGTACCAG CACGCCTTCA 
     4501 AGACCCCGGA TGCAGATGCC GGTGAAGAAA GAGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4561 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4621 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4681 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4741 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4801 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4861 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4921 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4981 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5041 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5101 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5161 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCTTGG 
     5221 CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 
     5281 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA 
     5341 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA 
     5401 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA 
     5461 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG 
     5521 TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA 
     5581 TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT 
     5641 GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA 
     5701 CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG 
     5761 GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG 
     5821 CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT 
     5881 GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT 
     5941 CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG 
     6001 CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT 
     6061 ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC 
     6121 GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC 
     6181 GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA 
     6241 TTCATCGACT GTGGCCGGCT GGGTGTGGCC GACCGCTATC AGGACATAGC GTTGGCTACC 
     6301 CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT 
     6361 ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA 
     6421 GCGGGACTCT GGGGTTCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT 
     6481 TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG 
     6541 GCTGGATGAT CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT 
     6601 TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG 
     6661 CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG 
     6721 TCTGTATACC GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG 
     6781 TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA 
     6841 AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG 
     6901 CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA 
     6961 GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG 
     7021 TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG 
     7081 AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC 
     7141 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA 
     7201 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT 
     7261 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC 
     7321 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC 
     7381 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC 
     7441 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT 
     7501 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG 
     7561 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA 
     7621 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA 
     7681 AACAAACCAC CGCTGGTAGC GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA 
     7741 AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA 
     7801 ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT 
     7861 TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA 
     7921 GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA 
     7981 TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC 
     8041 CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA 
     8101 ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC 
     8161 AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA 
     8221 ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT 
     8281 TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG 
     8341 CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC 
     8401 TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT 
     8461 CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT 
     8521 GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC 
     8581 TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT 
     8641 CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA 
     8701 GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA 
     8761 CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG 
     8821 GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG 
     8881 TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG 
     8941 CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG 
     9001 CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC 
     9061 TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA 
     9121 AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC 
     9181 CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC 
     9241 TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT 
     9301 GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//