LOCUS       RG235034                8790 bp    DNA     circular  SYN 
DEFINITION  TJP3 (tGFP-tagged) - Homo sapiens tight junction protein 3 (TJP3), transcript variant 1
ACCESSION   NM_001267560
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3269..3973 
                     /label="GFP"  
     misc_feature    4020..4605 
                     /label="PolyA signal"  
     misc_feature    6697..7342 
                     /label="ColE1"  
     misc_feature    5059..5853 
                     /label="Neo"  
     misc_feature    4690..4992 
                     /label="SV40 ori"  
     misc_feature    7357..8217 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3241..3248 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3241 
                     /label="TJP3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGAGCTG ACCATCTGGG AACAGCACAC GGCCACACTG TCCAAGGACC 
     1081 CCCGCCGGGG CTTTGGCATT GCGATCTCTG GAGGCCGAGA CCGGCCCGGT GGATCCATGG 
     1141 TTGTATCTGA CGTGGTACCT GGAGGGCCGG CGGAGGGCAG GCTACAGACA GGCGACCACA 
     1201 TCGTCATGGT GAACGGGGTT TCCATGGAGA ATGCCACCTC CGCGTTTGCC ATTCAGATAC 
     1261 TCAAGACCTG CACCAAGATG GCCAACATCA CAGTGAAACG TCCCCGGAGG ATCCACCTGC 
     1321 CCGCCACCAA AGCCAGCCCC TCCAGCCCAG GGCGCCAGGA CTCGGATGAA GACGATGGGC 
     1381 CCCAGCGGGT GGAGGAGGTG GACCAGGGCC GGGGCTATGA CGGCGACTCA TCCAGTGGCT 
     1441 CCGGCCGCTC CTGGGACGAG CGCTCCCGCC GGCCGAGGCC TGGTCGCCGG GGCCGGGCCG 
     1501 GCAGCCATGG GCGTAGGAGC CCAGGTGGTG GCTCTGAGGC CAACGGGCTG GCCCTGGTGT 
     1561 CCGGCTTTAA GCGGCTGCCA CGGCAGGACG TGCAGATGAA GCCTGTGAAG TCAGTGCTGG 
     1621 TGAAGAGGAG AGACAGCGAA GAGTTTGGCG TCAAGCTGGG CAGTCAGATC TTCATCAAGC 
     1681 ACATTACAGA TTCGGGCCTG GCTGCCCGGC ACCGTGGGCT GCAGGAAGGA GATCTCATTC 
     1741 TACAGATCAA CGGGGTGTCT AGCCAGAACC TGTCACTGAA CGACACCCGG CGACTGATTG 
     1801 AGAAGTCAGA AGGGAAGCTA AGCCTGCTGG TGCTGAGAGA TCGTGGGCAG TTCCTGGTGA 
     1861 ACATTCCGCC TGCTGTCAGT GACAGCGACA GCTCGCCATT GGAGGACATC TCGGACCTCG 
     1921 CCTCGGAGCT ATCGCAGGCA CCACCATCCC ACATCCCACC ACCACCCCGG CATGCTCAGC 
     1981 GGAGCCCCGA GGCCAGCCAG ACCGACTCTC CCGTGGAGAG TCCCCGGCTT CGGCGGGAAA 
     2041 GTTCAGTAGA TTCCAGAACC ATCTCGGAAC CAGATGAGCA ACGGTCAGAG TTGCCCAGGG 
     2101 AAAGCAGCTA TGACATCTAC AGAGTGCCCA GCAGTCAGAG CATGGAGGAT CGTGGGTACA 
     2161 GCCCCGACAC GCGTGTGGTC CGCTTCCTCA AGGGCAAGAG CATCGGGCTG CGGCTGGCAG 
     2221 GGGGCAATGA CGTGGGCATC TTCGTGTCCG GGGTGCAGGC GGGCAGCCCG GCCGACGGGC 
     2281 AGGGCATCCA GGAGGGAGAT CAGATTCTGC AGGTGAATGA CGTGCCATTC CAGAACCTGA 
     2341 CACGGGAGGA GGCAGTGCAG TTCCTGCTGG GGCTGCCACC AGGCGAGGAG ATGGAGCTGG 
     2401 TGACGCAGAG GAAGCAGGAC ATTTTCTGGA AAATGGTGCA GTCCCGCGTG GGTGACTCCT 
     2461 TCTACATCCG CACTCACTTT GAGCTGGAGC CCAGTCCACC GTCTGGCCTG GGCTTCACCC 
     2521 GTGGCGACGT CTTCCACGTG CTGGACACGC TGCACCCCGG CCCCGGGCAG AGCCACGCAC 
     2581 GAGGAGGCCA CTGGCTGGCG GTGCGCATGG GTCGTGACCT GCGGGAGCAA GAGCGGGGCA 
     2641 TCATTCCCAA CCAGAGCAGG GCGGAGCAGC TGGCCAGCCT GGAAGCTGCC CAGAGGGCCG 
     2701 TGGGAGTCGG GCCCGGCTCC TCCGCGGGCT CCAATGCTCG GGCCGAGTTC TGGCGGCTGC 
     2761 GGGGTCTTCG TCGAGGAGCC AAGAAGACCA CTCAGCGGAG CCGTGAGGAC CTCTCAGCTC 
     2821 TGACCCGACA GGGCCGCTAC CCGCCCTACG AACGAGTGGT GTTGCGAGAA GCCAGTTTCA 
     2881 AGCGCCCGGT AGTGATCCTG GGACCCGTGG CCGACATTGC TATGCAGAAG TTGACTGCTG 
     2941 AGATGCCTGA CCAGTTTGAA ATCGCAGAGA CTGTGTCCAG GACCGACAGC CCCTCCAAGA 
     3001 TCATCAAACT AGACACCGTG CGGGTGATTG CAGAAAAAGA CAAGCATGCG CTCCTGGATG 
     3061 TGACCCCCTC CGCCATCGAG CGCCTCAACT ATGTGCAGTA CTACCCCATT GTGGTCTTCT 
     3121 TCATCCCCGA GAGCCGGCCG GCCCTCAAGG CACTGCGCCA GTGGCTGGCG CCTGCCTCCC 
     3181 GCCGCAGCAC CCGTCGCCTC TACGCACAAG CCCAGAAGCT GCGAAAACAC AGCAGCCACC 
     3241 TCTTCACAGA GCGGACCGAC GCGTACGCGG CCGCTCGAGA TGGAGAGCGA CGAGAGCGGC 
     3301 CTGCCCGCCA TGGAGATCGA GTGCCGCATC ACCGGCACCC TGAACGGCGT GGAGTTCGAG 
     3361 CTGGTGGGCG GCGGAGAGGG CACCCCCGAG CAGGGCCGCA TGACCAACAA GATGAAGAGC 
     3421 ACCAAAGGCG CCCTGACCTT CAGCCCCTAC CTGCTGAGCC ACGTGATGGG CTACGGCTTC 
     3481 TACCACTTCG GCACCTACCC CAGCGGCTAC GAGAACCCCT TCCTGCACGC CATCAACAAC 
     3541 GGCGGCTACA CCAACACCCG CATCGAGAAG TACGAGGACG GCGGCGTGCT GCACGTGAGC 
     3601 TTCAGCTACC GCTACGAGGC CGGCCGCGTG ATCGGCGACT TCAAGGTGAT GGGCACCGGC 
     3661 TTCCCCGAGG ACAGCGTGAT CTTCACCGAC AAGATCATCC GCAGCAACGC CACCGTGGAG 
     3721 CACCTGCACC CCATGGGCGA TAACGATCTG GATGGCAGCT TCACCCGCAC CTTCAGCCTG 
     3781 CGCGACGGCG GCTACTACAG CTCCGTGGTG GACAGCCACA TGCACTTCAA GAGCGCCATC 
     3841 CACCCCAGCA TCCTGCAGAA CGGGGGCCCC ATGTTCGCCT TCCGCCGCGT GGAGGAGGAT 
     3901 CACAGCAACA CCGAGCTGGG CATCGTGGAG TACCAGCACG CCTTCAAGAC CCCGGATGCA 
     3961 GATGCCGGTG AAGAAAGAGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA 
     4021 TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC 
     4081 TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT 
     4141 GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA 
     4201 GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC 
     4261 TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA 
     4321 GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG 
     4381 ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC 
     4441 ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT 
     4501 TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG 
     4561 CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG 
     4621 GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA GCTTGGCTGT GGAATGTGTG 
     4681 TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA 
     4741 TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT 
     4801 GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC 
     4861 GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT 
     4921 TTATGCAGAG GCCGAGGCCG CCTCGGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT 
     4981 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC 
     5041 TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG 
     5101 TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG 
     5161 CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA 
     5221 GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT 
     5281 GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA 
     5341 CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC 
     5401 CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC 
     5461 CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC 
     5521 CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT 
     5581 GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA 
     5641 TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG 
     5701 CCGGCTGGGT GTGGCCGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA 
     5761 AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA 
     5821 TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG GACTCTGGGG 
     5881 TTCGAAATGA CCGACCAAGC GACGCCCAAC CTGCCATCAC GAGATTTCGA TTCCACCGCC 
     5941 GCCTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC 
     6001 CAGCGCGGGG ATCTCATGCT GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT 
     6061 AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG 
     6121 CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG 
     6181 ACCTCTAGCT AGAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT 
     6241 CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC 
     6301 TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA 
     6361 AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT 
     6421 ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG 
     6481 CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC 
     6541 GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG 
     6601 TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA 
     6661 AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC 
     6721 TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC 
     6781 CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG 
     6841 GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC 
     6901 TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA 
     6961 GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG 
     7021 AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG 
     7081 AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT 
     7141 GGTAGCGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA 
     7201 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG 
     7261 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA 
     7321 AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA 
     7381 ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC 
     7441 CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG 
     7501 ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA 
     7561 AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT 
     7621 TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT 
     7681 GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC 
     7741 CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC 
     7801 GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA 
     7861 GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG 
     7921 TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG 
     7981 TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA 
     8041 CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA 
     8101 CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA 
     8161 GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA 
     8221 ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8281 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8341 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8401 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8461 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8521 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8581 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8641 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8701 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8761 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//