LOCUS       RG235029                9308 bp    DNA     circular  SYN 
DEFINITION  SAFB (tGFP-tagged) - Homo sapiens scaffold attachment factor B (SAFB), transcript variant 2
ACCESSION   NM_001201339
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3789..4493 
                     /label="GFP"  
     misc_feature    4540..5125 
                     /label="PolyA signal"  
     misc_feature    7217..7862 
                     /label="ColE1"  
     misc_feature    5579..6373 
                     /label="Neo"  
     misc_feature    5210..5512 
                     /label="SV40 ori"  
     misc_feature    7877..8737 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3768..3774 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3768 
                     /label="SAFB"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGAGACT CTGTCAGGCC TAGGTGATTC TGGAGCGGCG GGCGCGGCGG 
     1081 CTCTGAGCTC CGCCTCGTCA GAGACCGGGA CGCGGCGCCT CAGCGACCTG CGAGTGATCG 
     1141 ATCTGCGGGC GGAGCTGAGG AAACGGAATG TGGACTCGAG CGGCAACAAG AGCGTTTTGA 
     1201 TGGAGCGGCT GAAGAAGGCA ATTGAAGATG AAGGTGGTAA TCCTGACGAA ATTGAAATTA 
     1261 CCTCCGAGGG AAACAAGAAA ACATCAAAGA GGTCTAGCAA AGGGCGCAAA CCAGAAGAAG 
     1321 AGGGTGTGGA AGATAACGGG CTGGAGGAAA ACTCTGGGGA TGGACAGGAG GATGTTGAGA 
     1381 CCAGTCTGGA GAACTTGCAG GACATCGACA TCATGGATAT CAGTGTGTTG GATGAAGCAG 
     1441 AAATTGATAA TGGAAGCGTT GCAGATTGTG TCGAAGACGA TGATGCTGAT AACCTCCAGG 
     1501 AGTCCCTGTC GGATAGTAGA GAGCTAGTCG AGGGGGAAAT GAAAGAGCTT CCGGAGCAGC 
     1561 TTCAGGAACA TGCTATAGAG GACAAAGAAA CTATAAACAA TTTAGATACT TCATCATCTG 
     1621 ACTTCACTAT ATTACAGGAA ATTGAAGAGC CATCCCTGGA GCCAGAAAAT GAGAAAATAC 
     1681 TCGACATTTT GGGGGAAACT TGTAAATCTG AGCCAGTAAA AGAAGAAAGT TCCGAGCTGG 
     1741 AGCAGCCATT TGCACAGGAC ACAAGTAGCG TGGGGCCAGA CAGAAAGCTT GCGGAGGAAG 
     1801 AGGACCTATT TGACAGCGCC CATCCGGAAG AGGGTGATTT AGATTTGGCC AGCGAGTCAA 
     1861 CAGCACACGC TCAGTCGAGC AAGGCAGACA GCCTGTTAGC GGTAGTGAAA AGGGAGCCCG 
     1921 CGGAGCAGCC AGGCGATGGC GAGAGGACGG ACTGTGAGCC TGTAGGGCTA GAGCCGGCAG 
     1981 TTGAGCAGAG TAGTGCGGCC TCCGAGCTCG CGGAGGCCTC TAGCGAGGAG CTCGCAGAAG 
     2041 CACCCACGGA AGCCCCAAGC CCAGAAGCCA GAGATAGCAA AGAAGACGGG AGGAAGTTTG 
     2101 ATTTTGACGC TTGTAATGAA GTCCCTCCGG CTCCTAAAGA GTCCTCAACC AGTGAGGGCG 
     2161 CTGATCAGAA AATGAGTTCT CCCGAAGATG ACTCGGATAC AAAAAGGCTT TCCAAAGAGG 
     2221 AAAAGGGTCG CAGCAGTTGT GGTAGAAATT TCTGGGTTAG TGGACTCTCT TCTACAACCA 
     2281 GAGCTACAGA TTTGAAGAAT CTTTTCAGCA AATATGGGAA GGTGGTGGGC GCCAAGGTTG 
     2341 TGACAAATGC CCGGAGTCCT GGAGCTCGCT GTTACGGTTT TGTCACGATG TCCACAGCAG 
     2401 AAGAGGCCAC AAAATGCATT AACCACCTGC ACAAGACGGA GCTCCACGGA AAGATGATCT 
     2461 CCGTGGAGAA AGCCAAAAAT GAACCTGTGG GAAAGAAAAC CTCTGACAAA AGAGACAGTG 
     2521 ACGGGAAAAA GGAGAAGTCG AGCAACAGTG ACAGATCTAC AAACCTTAAG AGGGATGATA 
     2581 AATGTGACAG AAAAGATGAT GCTAAGAAGG GTGACGACGG AAGTGGAGAA AAGAGTAAGG 
     2641 ACCAAGATGA TCAGAAACCT GGCCCCTCAG AGCGATCTCG AGCCACAAAG TCAGGAAGTC 
     2701 GAGGGACCGA ACGGACTGTA GTAATGGATA AATCCAAAGG GGTGCCTGTG ATTAGTGTAA 
     2761 AAACGTCCGG GTCCAAAGAG AGAGCTTCCA AAAGCCAGGA TCGCAAATCA GCCAGCAGAG 
     2821 AGAAGCGGTC CGTCGTGTCC TTTGATAAGG TCAAGGAGCC TCGGAAGTCA AGAGACTCAG 
     2881 AGTCCCATAG GGTGCGTGAA CGCAGTGAAC GCGAACAACG CATGCAGGCG CAGTGGGAGC 
     2941 GCGAGGAGCG TGAGCGGCTG GAGATTGCCC GAGAGAGGCT GGCCTTCCAG CGCCAGCGGC 
     3001 TGGAGCGGGA GCGCATGGAG CGGGAACGGC TGGAGCGCGA ACGCATGCAC GTGGAGCACG 
     3061 AGCGCAGGCG CGAGCAGGAG CGCATCCACC GTGAGCGCGA GGAGCTGAGG CGCCAGCAGG 
     3121 AACTGCGCTA TGAGCAGGAG CGGCGGCCCG CGGTGCGGCG GCCCTACGAC CTGGACCGGC 
     3181 GAGATGATGC CTATTGGCCG GAAGCCAAGC GGGCCGCCCT GGATGAGCGC TACCATTCTG 
     3241 ACTTTAACCG CCAGGACCGC TTCCACGACT TTGACCACAG GGACCGCGGC CGCTACCCCG 
     3301 ACCACTCGGT GGACAGGAGA GAAGGTTCAA GGTCAATGAT GGGAGAACGA GAAGGACAGC 
     3361 ATTACCCAGA ACGCCATGGA GGACCAGAGC GCCACGGCCG GGACTCCCGC GATGGCTGGG 
     3421 GGGGCTATGG CTCTGACAAG AGGATGAGCG AGGGCCGGGG GCTGCCTCCT CCCCCCAGGG 
     3481 GCAGACGTGA CTGGGGGGAC CATGGCCGAA GAGAGGATGA CCGGTCATGG CAGGGCACGG 
     3541 CCGACGGGGG CATGATGGAC AGGGATCACA AGAGGTGGCA AGGTGGCGAG AGAAGCATGT 
     3601 CCGGTCACTC CGGGCCTGGC CACATGATGA ACCGAGGAGG AATGTCAGGG CGCGGCAGCT 
     3661 TTGCCCCAGG CGGGGCCTCC CGGGGCCACC CCATCCCACA CGGTGGCATG CAGGGCGGGT 
     3721 TTGGAGGCCA GAGCCGGGGG AGCAGGCCCA GCGATGCCCG CTTCACTCGC CGCTACACGC 
     3781 GTACGCGGCC GCTCGAGATG GAGAGCGACG AGAGCGGCCT GCCCGCCATG GAGATCGAGT 
     3841 GCCGCATCAC CGGCACCCTG AACGGCGTGG AGTTCGAGCT GGTGGGCGGC GGAGAGGGCA 
     3901 CCCCCGAGCA GGGCCGCATG ACCAACAAGA TGAAGAGCAC CAAAGGCGCC CTGACCTTCA 
     3961 GCCCCTACCT GCTGAGCCAC GTGATGGGCT ACGGCTTCTA CCACTTCGGC ACCTACCCCA 
     4021 GCGGCTACGA GAACCCCTTC CTGCACGCCA TCAACAACGG CGGCTACACC AACACCCGCA 
     4081 TCGAGAAGTA CGAGGACGGC GGCGTGCTGC ACGTGAGCTT CAGCTACCGC TACGAGGCCG 
     4141 GCCGCGTGAT CGGCGACTTC AAGGTGATGG GCACCGGCTT CCCCGAGGAC AGCGTGATCT 
     4201 TCACCGACAA GATCATCCGC AGCAACGCCA CCGTGGAGCA CCTGCACCCC ATGGGCGATA 
     4261 ACGATCTGGA TGGCAGCTTC ACCCGCACCT TCAGCCTGCG CGACGGCGGC TACTACAGCT 
     4321 CCGTGGTGGA CAGCCACATG CACTTCAAGA GCGCCATCCA CCCCAGCATC CTGCAGAACG 
     4381 GGGGCCCCAT GTTCGCCTTC CGCCGCGTGG AGGAGGATCA CAGCAACACC GAGCTGGGCA 
     4441 TCGTGGAGTA CCAGCACGCC TTCAAGACCC CGGATGCAGA TGCCGGTGAA GAAAGAGTTT 
     4501 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4561 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4621 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4681 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4741 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4801 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4861 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4921 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     4981 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5041 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5101 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5161 GAATTGGGTA CGCGGCCAGC TTGGCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC 
     5221 CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG 
     5281 TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG 
     5341 TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC 
     5401 GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC 
     5461 TCGGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC 
     5521 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAAGAGAC AGGATGAGGA 
     5581 TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG 
     5641 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC 
     5701 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG 
     5761 AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC 
     5821 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG 
     5881 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT 
     5941 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG 
     6001 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT 
     6061 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC 
     6121 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG 
     6181 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCCGACCGC 
     6241 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT 
     6301 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT 
     6361 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA 
     6421 CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT 
     6481 TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG 
     6541 AGTTCTTCGC CCACCCCAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA 
     6601 GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA 
     6661 AACTCATCAA TGTATCTTAT CATGTCTGTA TACCGTCGAC CTCTAGCTAG AGCTTGGCGT 
     6721 AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA 
     6781 TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT 
     6841 TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT 
     6901 AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT 
     6961 CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA 
     7021 AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA 
     7081 AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC 
     7141 TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA 
     7201 CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC 
     7261 CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT 
     7321 CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT 
     7381 GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG 
     7441 AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA 
     7501 GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT 
     7561 ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA 
     7621 GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTTTT TTTGTTTGCA 
     7681 AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG 
     7741 GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA 
     7801 AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA 
     7861 TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG 
     7921 CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA 
     7981 TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC 
     8041 CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC 
     8101 CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA 
     8161 GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC 
     8221 GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT 
     8281 GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA 
     8341 GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG 
     8401 TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG 
     8461 AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC 
     8521 CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT 
     8581 CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT 
     8641 CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG 
     8701 CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC 
     8761 AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA 
     8821 TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG 
     8881 CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA 
     8941 CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT 
     9001 TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG 
     9061 CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT 
     9121 CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC 
     9181 TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG 
     9241 GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG 
     9301 CGAATTTT
//