LOCUS       RG235012                9269 bp    DNA     circular  SYN 
DEFINITION  AKAP1 (tGFP-tagged) - Homo sapiens A kinase (PRKA) anchor protein 1 (AKAP1), transcript variant 2
ACCESSION   NM_001242902
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3750..4454 
                     /label="GFP"  
     misc_feature    4501..5086 
                     /label="PolyA signal"  
     misc_feature    7178..7823 
                     /label="ColE1"  
     misc_feature    5540..6334 
                     /label="Neo"  
     misc_feature    5171..5473 
                     /label="SV40 ori"  
     misc_feature    7838..8698 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3729..3735 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3729 
                     /label="AKAP1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAATCCAG TTCCGTTCGC TCTTCCCCTT GGCATTGCCT GGGATGCTGG 
     1081 CGCTCCTCGG CTGGTGGTGG TTTTTCTCTC GTAAAAAAGG CCATGTCAGC AGCCATGATG 
     1141 AGCAGCAGGT GGAGGCTGGT GCTGTGCAGC TGAGGGCTGA CCCTGCCATC AAGGAACCTC 
     1201 TCCCCGTGGA AGACGTCTGT CCCAAAGTAG TGTCCACACC CCCCAGTGTC ACAGAGCCTC 
     1261 CAGAAAAGGA ACTGTCCACC GTGAGCAAGC TGCCTGCAGA GCCCCCAGCA TTGCTCCAGA 
     1321 CACACCCACC TTGCCGAAGA TCAGAGTCCT CGGGCATTCT TCCTAACACC ACAGACATGA 
     1381 GATTGCGACC AGGAACACGC AGAGATGACA GTACAAAGCT GGAGCTAGCC CTGACAGGTG 
     1441 GTGAAGCCAA ATCGATTCCT CTAGAGTGCC CCCTTTCATC CCCAAAGGGT GTACTATTCT 
     1501 CCAGCAAATC AGCTGAGGTG TGTAAGCAAG ATTCCCCCTT CAGCAGGGTG CCAAGGAAGG 
     1561 TCCAGCCAGG CTACCCCGTA GTCCCCGCAG AGAAGCGTAG CTCTGGGGAG AGGGCAAGAG 
     1621 AGACAGGTGG GGCCGAAGGG ACTGGTGATG CCGTGTTGGG GGAAAAGGTG CTTGAAGAAG 
     1681 CTCTGTTGTC TCGGGAGCAT GTCTTGGAAT TGGAGAACAG CAAGGGCCCC AGCCTGGCCT 
     1741 CTTTAGAGGG GGAAGAAGAT AAGGGGAAGA GCAGCTCATC CCAGGTGGTG GGGCCAGTGC 
     1801 AGGAGGAAGA GTATGTAGCA GAGAAGTTGC CAAGTAGGTT CATCGAGTCG GCTCACACAG 
     1861 AGCTGGCAAA GGACGATGCG GCGCCAGCAC CCCCAGTCGC AGACGCCAAA GCCCAGGATA 
     1921 GAGGTGTCGA GGGAGAACTG GGCAATGAGG AGAGCTTGGA TAGAAATGAG GAGGGCTTGG 
     1981 ATAGAAATGA GGAGGGCTTG GATAGAAATG AGGAGAGCTT GGATAGAAAT GAGGAGGGCT 
     2041 TGGATAGAAA TGAGGAGATT AAGCGGGCTG CCTTCCAGAT AATCTCCCAA GTGATCTCAG 
     2101 AAGCAACCGA ACAGGTGCTG GCCACCACGG TTGGCAAGGT TGCAGGTCGT GTGTGTCAGG 
     2161 CCAGTCAGCT CCAAGGGCAG AAGGAAGAGA GCTGTGTCCC AGTTCACCAG AAAACTGTCT 
     2221 TGGGCCCAGA CACTGCGGAG CCTGCCACAG CAGAGGCAGC TGTTGCCCCG CCGGATGCTG 
     2281 GCCTCCCCTT GCCAGGCCTA CCAGCAGAGG GCTCACCACC ACCAAAGACC TACGTGAGCT 
     2341 GCCTGAAGAG CCTTCTGTCC AGCCCCACCA AGGACAGTAA GCCAAATATC TCTGCACACC 
     2401 ACATCTCCCT GGCCTCCTGC CTGGCACTGA CCACCCCCAG TGAAGAGTTG CCGGACCGGG 
     2461 CAGGCATCCT GGTGGAAGAT GCCACCTGTG TCACCTGCAT GTCAGACAGC AGCCAAAGTG 
     2521 TCCCTTTGGT GGCTTCTCCA GGACACTGCT CAGATTCTTT CAGCACTTCA GGGCTTGAAG 
     2581 ACTCTTGCAC AGAGACCAGC TCGAGCCCCA GGGACAAGGC CATCACCCCG CCACTGCCAG 
     2641 AAAGTACTGT GCCCTTCAGC AATGGGGTGC TGAAGGGGGA GTTGTCAGAC TTGGGGGCTG 
     2701 AGGATGGATG GACCATGGAT GCGGAAGCAG ATCATTCAGG AGGTTCTGAC AGGAACAGCA 
     2761 TGGATTCCGT GGATAGCTGT TGCAGTCTCA AGAAGACTGA GAGCTTCCAA AATGCCCAGG 
     2821 CAGGCTCCAA CCCTAAGAAG GTCGACCTCA TCATCTGGGA GATCGAGGTG CCAAAGCACT 
     2881 TAGTCGGTCG GCTAATTGGC AAGCAGGGGC GCTATGTGAG TTTTCTGAAG CAAACATCTG 
     2941 GTGCCAAGAT CTACATTTCA ACCCTGCCTT ACACCCAGAG CGTCCAGATC TGCCACATAG 
     3001 AAGGCTCTCA ACATCATGTA GACAAAGCGC TGAACTTGAT TGGGAAGAAG TTCAAAGAGC 
     3061 TGAACCTCAC CAATATCTAC GCTCCCCCAT TGCCTTCACT GGCACTGCCT TCTCTGCCGA 
     3121 TGACATCCTG GCTCATGCTG CCTGATGGCA TCACCGTGGA GGTCATTGTG GTCAACCAGG 
     3181 TCAATGCCGG GCACCTGTTC GTGCAGCAGC ACACACACCC TACCTTCCAC GCGCTGCGCA 
     3241 GCCTCGACCA GCAGATGTAC CTCTGTTACT CTCAGCCTGG AATCCCCACC TTGCCCACCC 
     3301 CAGTGGAAAT AACGGTCATC TGTGCCGCCC CTGGTGCGGA CGGGGCCTGG TGGCGAGCCC 
     3361 AAGTGGTTGC CTCCTACGAG GAGACCAACG AAGTGGAGAT TCGATACGTG GACTACGGCG 
     3421 GATATAAGAG GGTGAAAGTA GACGTGCTCC GGCAAATCAG GTCTGACTTT GTCACCCTGC 
     3481 CGTTTCAGGG AGCAGAAGTC CTTCTGGACA GTGTGATGCC CCTGTCAGAC GATGACCAGT 
     3541 TTTCACCGGA AGCAGATGCC GCCATGAGCG AGATGACGGG GAATACAGCA CTGCTTGCTC 
     3601 AGGTGACAAG TTACAGTCCA ACTGGTCTTC CTCTGATTCA GCTGTGGAGT GTGGTTGGAG 
     3661 ATGAAGTGGT GTTGATAAAC CGGTCCCTGG TGGAGCGAGG CCTTGCCCAG TGGGTAGACA 
     3721 GCTACTACAC AAGCCTTACG CGTACGCGGC CGCTCGAGAT GGAGAGCGAC GAGAGCGGCC 
     3781 TGCCCGCCAT GGAGATCGAG TGCCGCATCA CCGGCACCCT GAACGGCGTG GAGTTCGAGC 
     3841 TGGTGGGCGG CGGAGAGGGC ACCCCCGAGC AGGGCCGCAT GACCAACAAG ATGAAGAGCA 
     3901 CCAAAGGCGC CCTGACCTTC AGCCCCTACC TGCTGAGCCA CGTGATGGGC TACGGCTTCT 
     3961 ACCACTTCGG CACCTACCCC AGCGGCTACG AGAACCCCTT CCTGCACGCC ATCAACAACG 
     4021 GCGGCTACAC CAACACCCGC ATCGAGAAGT ACGAGGACGG CGGCGTGCTG CACGTGAGCT 
     4081 TCAGCTACCG CTACGAGGCC GGCCGCGTGA TCGGCGACTT CAAGGTGATG GGCACCGGCT 
     4141 TCCCCGAGGA CAGCGTGATC TTCACCGACA AGATCATCCG CAGCAACGCC ACCGTGGAGC 
     4201 ACCTGCACCC CATGGGCGAT AACGATCTGG ATGGCAGCTT CACCCGCACC TTCAGCCTGC 
     4261 GCGACGGCGG CTACTACAGC TCCGTGGTGG ACAGCCACAT GCACTTCAAG AGCGCCATCC 
     4321 ACCCCAGCAT CCTGCAGAAC GGGGGCCCCA TGTTCGCCTT CCGCCGCGTG GAGGAGGATC 
     4381 ACAGCAACAC CGAGCTGGGC ATCGTGGAGT ACCAGCACGC CTTCAAGACC CCGGATGCAG 
     4441 ATGCCGGTGA AGAAAGAGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4501 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4561 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4621 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4681 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4741 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4801 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4861 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4921 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4981 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5041 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5101 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CTTGGCTGTG GAATGTGTGT 
     5161 CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 
     5221 CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG 
     5281 CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG 
     5341 CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT 
     5401 TATGCAGAGG CCGAGGCCGC CTCGGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT 
     5461 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT 
     5521 GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT 
     5581 TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC 
     5641 TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG 
     5701 ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG 
     5761 GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC 
     5821 TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC 
     5881 GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC 
     5941 TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC 
     6001 GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG 
     6061 TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT 
     6121 GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC 
     6181 CGGCTGGGTG TGGCCGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA 
     6241 GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT 
     6301 TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT 
     6361 TCGAAATGAC CGACCAAGCG ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG 
     6421 CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC 
     6481 AGCGCGGGGA TCTCATGCTG GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA 
     6541 ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC 
     6601 ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA 
     6661 CCTCTAGCTA GAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC 
     6721 CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT 
     6781 AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA 
     6841 ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA 
     6901 TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC 
     6961 GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG 
     7021 CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT 
     7081 TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA 
     7141 GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT 
     7201 CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC 
     7261 CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG 
     7321 TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT 
     7381 TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG 
     7441 CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA 
     7501 AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA 
     7561 AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG 
     7621 GTAGCGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG 
     7681 ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA 
     7741 TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA 
     7801 GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA 
     7861 TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC 
     7921 CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA 
     7981 TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA 
     8041 GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT 
     8101 GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG 
     8161 CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC 
     8221 AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG 
     8281 GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG 
     8341 CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT 
     8401 ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT 
     8461 CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC 
     8521 GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC 
     8581 CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG 
     8641 CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA 
     8701 TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA 
     8761 GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC 
     8821 CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG 
     8881 TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT 
     8941 TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC 
     9001 CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG 
     9061 ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT 
     9121 CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG 
     9181 TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC 
     9241 TGATTTAACA AAAATTTAAC GCGAATTTT
//