LOCUS       RG235001                9248 bp    DNA     circular  SYN 
DEFINITION  LPIN1 (tGFP-tagged) - Homo sapiens lipin 1 (LPIN1), transcript variant 2
ACCESSION   NM_001261427
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3729..4433 
                     /label="GFP"  
     misc_feature    4480..5065 
                     /label="PolyA signal"  
     misc_feature    7157..7802 
                     /label="ColE1"  
     misc_feature    5519..6313 
                     /label="Neo"  
     misc_feature    5150..5452 
                     /label="SV40 ori"  
     misc_feature    7817..8677 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3708..3714 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3708 
                     /label="LPIN1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGCAGAGTG CAGACCATGA ATTACGTGGG GCAGTTAGCC GGCCAGGTGT 
     1081 TTGTCACCGT GAAGGAGCTC TACAAGGGGC TGAATCCCGC CACACTCTCA GGGTGCATTG 
     1141 ACATCATTGT CATCCGCCAG CCCAATGGAA ACCTCCAATG CTCCCCTTTC CACGTCCGCT 
     1201 TTGGGAAGAT GGGGGTCCTG CGCTCCCGAG AGAAAGTGGT TGACATAGAA ATCAATGGGG 
     1261 AATCTGTGGA TTTGCATATG AAATTGGGAG ATAATGGAGA AGCATTTTTT GTTCAAGAAA 
     1321 CAGATAATGA TCAGGAAGTT ATCCCTATGC ACCTGGCCAC CTCCCCCATC CTGTCAGAAG 
     1381 GAGCTTCGAG AATGGAATGC CAGCTGAAAA GGGGCTCTGT GGACAGGATG AGAGGCCTGG 
     1441 ACCCCAGCAC GCCAGCCCAA GTGATCGCTC CCAGCGAGAC GCCGTCAAGC AGCTCTGTAG 
     1501 TAAAGAAGAG AAGAAAAAGG AGGAGAAAGT CACAGCTGGA CAGCCTGAAG AGAGATGACA 
     1561 ACATGAACAC ATCTGAGGAT GAGGACATGT TCCCCATCGA GATGAGCTCG GATGAGGCCA 
     1621 TGGAGCTGCT GGAGAGCAGC AGAACTCTTC CTAATGATAT ACCTCCATTC CAAGATGATA 
     1681 TTCCTGAGGA AAACCTCTCC CTGGCTGTGA TTTACCCTCA GTCAGCCTCA TACCCTAATT 
     1741 CGGATAGAGA GTGGTCACCC ACTCCCAGTC CTTCCGGTTC CCGACCTTCA ACACCTAAAA 
     1801 GTGATTCAGA ATTGGTCAGC AAGTCCACGG AAAGGACAGG GCAGAAGAAC CCAGAAATGC 
     1861 TTTGGCTGTG GGGAGAGCTG CCGCAGGCTG CTAAGTCTTC TTCTCCACAC AAGATGAAAG 
     1921 AGTCCAGCCC ATTGAGCAGT AGAAAAATTT GTGATAAAAG TCACTTTCAG GCCATTCACA 
     1981 GCGAATCTTC AGACACTTTT AGTGACCAAT CGCCAACTCT GGTCGGTGGG GCACTTTTGG 
     2041 ACCAGAACAA GCCTCAGACA GAAATGCAGT TTGTGAATGA AGAAGACCTG GAGACCTTAG 
     2101 GAGCAGCAGC GCCACTCTTG CCCATGATCG AGGAGCTCAA ACCCCCCTCT GCCAGTGTAG 
     2161 TCCAGACAGC AAACAAGACG GATTCTCCTT CCAGGAAAAG AGATAAACGA AGCCGACATC 
     2221 TTGGTGCTGA CGGCGTCTAC TTGGATGACC TCACAGACAT GGATCCTGAA GTGGCGGCCC 
     2281 TGTATTTTCC CAAAAACGGA GATCCTTCCG GACTCGCAAA ACATGCAAGC GACAACGGAG 
     2341 CCCGGTCAGC CAACCAGTCC CCGCAGTCGG TGGGCAGCTC GGGCGTGGAC AGTGGCGTGG 
     2401 AGAGCACCTC GGACGGGCTG AGGGACCTCC CTTCCATCGC CATCTCCCTC TGCGGGGGCC 
     2461 TCAGCGACCA CCGGGAGATC ACGAAAGATG CATTCCTGGA GCAAGCTGTG TCATATCAAC 
     2521 AGTTTGTGGA CAACCCCGCT ATTATCGATG ACCCCAATCT CGTGGTAAAG ATTGGGAGTA 
     2581 AATATTATAA CTGGACAACA GCAGCACCCC TCCTCCTGGC AATGCAGGCC TTCCAGAAAC 
     2641 CTTTGCCAAA GGCCACTGTG GAATCTATCA TGAGGGATAA AATGCCCAAA AAGGGAGGAA 
     2701 GATGGTGGTT TTCATGGAGG GGAAGAAACA CCACAATCAA GGAGGAAAGT AAGCCAGAGC 
     2761 AGTGCTTGGC TGGCAAGGCC CATAGCACCG GAGAGCAACC GCCGCAGCTC AGCTTGGCCA 
     2821 CCAGGGTAAA GCATGAATCA TCCTCCAGTG ATGAGGAGCG CGCAGCTGCC AAGCCATCAA 
     2881 ACGCAGGCCA CCTCCCTCTT CTGCCTAATG TCAGCTACAA GAAGACTCTC CGGCTGACTT 
     2941 CCGAGCAGCT TAAAAGCTTG AAGTTGAAGA ATGGCCCCAA CGACGTGGTT TTCAGTGTCA 
     3001 CCACGCAGTA CCAAGGCACG TGCCGCTGTG AGGGCACCAT CTATCTGTGG AACTGGGATG 
     3061 ATAAAGTCAT CATTTCTGAT ATTGATGGGA CAATTACCAG ATCAGATACT CTTGGCCACA 
     3121 TTTTGCCCAC CCTTGGGAAG GATTGGACCC ATCAGGGCAT CGCTAAGCTG TACCATAAAG 
     3181 TGAGCCAGAA TGGATATAAA TTTCTCTACT GTTCTGCCCG TGCCATCGGG ATGGCGGACA 
     3241 TGACGCGGGG CTACCTGCAC TGGGTCAACG AGAGGGGCAC GGTGCTGCCC CAGGGGCCCC 
     3301 TGCTGCTGAG TCCCAGCAGC CTCTTCTCTG CCCTGCACAG AGAAGTGATT GAAAAGAAGC 
     3361 CAGAAAAGTT TAAAGTCCAG TGTTTGACAG ACATCAAAAA CCTGTTTTTC CCCAACACAG 
     3421 AACCCTTTTA TGCTGCTTTT GGAAACCGAC CAGCTGATGT GTATTCATAC AAGCAAGTAG 
     3481 GAGTGTCTTT GAATAGAATA TTTACCGTCA ACCCTAAAGG AGAGCTGGTA CAGGAACATG 
     3541 CAAAGACCAA CATCTCTTCG TATGTGAGAC TCTGTGAAGT AGTCGACCAC GTTTTCCCGT 
     3601 TGCTGAAAAG AAGCCATTCT TCAGACTTTC CCTGTTCGGA TACCTTCAGT AACTTCACCT 
     3661 TTTGGAGAGA GCCACTGCCA CCTTTTGAAA ACCAGGACAT TCATTCTGCC TCAGCGACGC 
     3721 GTACGCGGCC GCTCGAGATG GAGAGCGACG AGAGCGGCCT GCCCGCCATG GAGATCGAGT 
     3781 GCCGCATCAC CGGCACCCTG AACGGCGTGG AGTTCGAGCT GGTGGGCGGC GGAGAGGGCA 
     3841 CCCCCGAGCA GGGCCGCATG ACCAACAAGA TGAAGAGCAC CAAAGGCGCC CTGACCTTCA 
     3901 GCCCCTACCT GCTGAGCCAC GTGATGGGCT ACGGCTTCTA CCACTTCGGC ACCTACCCCA 
     3961 GCGGCTACGA GAACCCCTTC CTGCACGCCA TCAACAACGG CGGCTACACC AACACCCGCA 
     4021 TCGAGAAGTA CGAGGACGGC GGCGTGCTGC ACGTGAGCTT CAGCTACCGC TACGAGGCCG 
     4081 GCCGCGTGAT CGGCGACTTC AAGGTGATGG GCACCGGCTT CCCCGAGGAC AGCGTGATCT 
     4141 TCACCGACAA GATCATCCGC AGCAACGCCA CCGTGGAGCA CCTGCACCCC ATGGGCGATA 
     4201 ACGATCTGGA TGGCAGCTTC ACCCGCACCT TCAGCCTGCG CGACGGCGGC TACTACAGCT 
     4261 CCGTGGTGGA CAGCCACATG CACTTCAAGA GCGCCATCCA CCCCAGCATC CTGCAGAACG 
     4321 GGGGCCCCAT GTTCGCCTTC CGCCGCGTGG AGGAGGATCA CAGCAACACC GAGCTGGGCA 
     4381 TCGTGGAGTA CCAGCACGCC TTCAAGACCC CGGATGCAGA TGCCGGTGAA GAAAGAGTTT 
     4441 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4501 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4561 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4621 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4681 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4741 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4801 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4861 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     4921 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     4981 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5041 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5101 GAATTGGGTA CGCGGCCAGC TTGGCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC 
     5161 CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG 
     5221 TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG 
     5281 TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC 
     5341 GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC 
     5401 TCGGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC 
     5461 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAAGAGAC AGGATGAGGA 
     5521 TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG 
     5581 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC 
     5641 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG 
     5701 AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC 
     5761 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG 
     5821 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT 
     5881 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG 
     5941 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT 
     6001 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC 
     6061 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG 
     6121 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCCGACCGC 
     6181 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT 
     6241 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT 
     6301 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA 
     6361 CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT 
     6421 TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG 
     6481 AGTTCTTCGC CCACCCCAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA 
     6541 GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA 
     6601 AACTCATCAA TGTATCTTAT CATGTCTGTA TACCGTCGAC CTCTAGCTAG AGCTTGGCGT 
     6661 AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA 
     6721 TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT 
     6781 TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT 
     6841 AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT 
     6901 CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA 
     6961 AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA 
     7021 AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC 
     7081 TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA 
     7141 CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC 
     7201 CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT 
     7261 CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT 
     7321 GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG 
     7381 AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA 
     7441 GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT 
     7501 ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA 
     7561 GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTTTT TTTGTTTGCA 
     7621 AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG 
     7681 GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA 
     7741 AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA 
     7801 TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG 
     7861 CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA 
     7921 TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC 
     7981 CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC 
     8041 CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA 
     8101 GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC 
     8161 GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT 
     8221 GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA 
     8281 GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG 
     8341 TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG 
     8401 AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC 
     8461 CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT 
     8521 CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT 
     8581 CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG 
     8641 CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC 
     8701 AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA 
     8761 TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG 
     8821 CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA 
     8881 CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT 
     8941 TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG 
     9001 CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT 
     9061 CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC 
     9121 TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG 
     9181 GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG 
     9241 CGAATTTT
//