LOCUS       RG234998                9245 bp    DNA     circular  SYN 
DEFINITION  LONP1 (tGFP-tagged) - Homo sapiens lon peptidase 1, mitochondrial (LONP1), transcript variant 2
ACCESSION   NM_001276479
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3726..4430 
                     /label="GFP"  
     misc_feature    4477..5062 
                     /label="PolyA signal"  
     misc_feature    7154..7799 
                     /label="ColE1"  
     misc_feature    5516..6310 
                     /label="Neo"  
     misc_feature    5147..5449 
                     /label="SV40 ori"  
     misc_feature    7814..8674 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3705..3711 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3705 
                     /label="LONP1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCGAGC ACTGGCTACG TGCGACTGTG GGGAGCGGCG CGGTGCTGGG 
     1081 TGCTGCGGCG GCCGATGCTG GCCGCCGCCG GGGGGCGGGT TCCCACTGCA GCAGGAGCGT 
     1141 GGTTGCTCCG AGGCCCGGTC ATAACGGCGC TCACGCCCAT GACGATCCCC GATGTGTTTC 
     1201 CGCACCTGCC GCTCATCGCC ATCACCCGCA ACCCGGTGTT CCCGCGCTTT ATCAAGATTA 
     1261 TCGAGGTTAA AAATAAGAAG TTGGTTGAGC TGCTGAGAAG GAAAGTTCGT CTCGCCCAGC 
     1321 CTTATGTCGG CGTCTTTCTA AAGAGAGATG ACAGCAATGA GTCGGATGTG GTCGAGAGCC 
     1381 TGGATGAAAT CTACCACACG GGGACGTTTG CCCAGATCCA TGAGATGCAG GACCTTGGGG 
     1441 ACAAGCTGCG CATGATCGTC ATGGGACACA GAAGAGTCCA TATCAGCAGA CAGCTGGAGG 
     1501 TGGAGCCCGA GGAGCCGGAG GCGGAGAACA AGCACAAGCC CCGCAGGAAG TCAAAGCGGG 
     1561 GCAAGAAGGA GGCGGAGGAC GAGCTGAGCG CCAGGCACCC GGCGGAGCTG GCGATGGAGC 
     1621 CCACCCCTGA GCTCCCGGCT GAGGTGCTCA TGGTGGAGGT AGAGAACGTT GTCCACGAGG 
     1681 ACTTCCAGGT CACGGAGGAG GTGAAAGCCC TGACTGCAGA GATCGTGAAG ACCATCCGGG 
     1741 ACATCATTGC CTTGAACCCT CTCTACAGGG AGTCAGTGCT GCAGATGATG CAGGCTGGCC 
     1801 AGCGGGTGGT GGACAACCCC ATCTACCTGA GCGACATGGG CGCCGCGCTC ACCGGGGCCG 
     1861 AGTCCCATGA GCTGCAGGAC GTCCTGGAAG AGACCAATAT TCCTAAGCGG CTGTACAAGG 
     1921 CCCTCTCCCT GCTGAAGAAG GAATTTGAAC TGAGCAAGCT GCAGCAGCGC CTGGGGCGGG 
     1981 AGGTGGAGGA GAAGATCAAG CAGACCCACC GTAAGTACCT GCTGCAGGAG CAGCTAAAGA 
     2041 TCATCAAGAA GGAGCTGGGC CTGGAGAAGG ACGACAAGGA TGCCATCGAG GAGAAGTTCC 
     2101 GGGAGCGCCT GAAGGAGCTC GTGGTCCCCA AGCACGTCAT GGATGTTGTG GACGAGGAGC 
     2161 TGAGCAAGCT GGGCCTGCTG GACAACCACT CCTCGGAGTT CAATGTCACC CGCAACTACC 
     2221 TAGACTGGCT CACGTCCATC CCTTGGGGCA AGTACAGCAA CGAGAACCTG GACCTGGCGC 
     2281 GGGCACAGGC AGTGCTGGAG GAAGACCACT ACGGCATGGA GGACGTCAAG AAACGCATCC 
     2341 TGGAGTTCAT TGCCGTTAGC CAGCTCCGCG GCTCCACCCA GGGCAAGATC CTCTGCTTCT 
     2401 ATGGCCCCCC TGGCGTGGGT AAGACCAGCA TTGCTCGCTC CATCGCCCGC GCCCTGAACC 
     2461 GAGAGTACTT CCGCTTCAGC GTCGGGGGCA TGACTGACGT GGCTGAGATC AAGGGCCACA 
     2521 GGCGGACCTA CGTGGGCGCC ATGCCCGGGA AGATCATCCA GTGTTTGAAG AAGACCAAGA 
     2581 CGGAGAACCC CCTGATCCTC ATCGACGAGG TGGACAAGAT CGGCCGAGGC TACCAGGGGG 
     2641 ACCCGTCGTC GGCACTGCTG GAGCTGCTGG ACCCAGAGCA GAATGCCAAC TTCCTGGACC 
     2701 ACTACCTGGA CGTGCCCGTG GACTTGTCCA AGGTGCTGTT CATCTGCACG GCCAACGTCA 
     2761 CGGACACCAT CCCCGAGCCG CTGCGAGACC GTATGGAGAT GATCAACGTG TCGGGCTACG 
     2821 TGGCCCAGGA GAAGCTGGCC ATTGCGGAGC GCTACCTGGT GCCCCAGGCT CGCGCCCTGT 
     2881 GTGGCTTGGA TGAGAGCAAG GCCAAGCTGT CATCGGACGT GCTGACGCTG CTCATCAAGC 
     2941 AGTACTGCCG CGAGAGCGGT GTCCGCAACC TGCAGAAGCA AGTGGAGAAG GTGTTACGGA 
     3001 AATCGGCCTA CAAGATTGTC AGCGGCGAGG CCGAGTCCGT GGAGGTGACG CCCGAGAACC 
     3061 TGCAGGACTT CGTGGGGAAG CCCGTGTTCA CCGTGGAGCG CATGTATGAC GTGACACCGC 
     3121 CCGGCGTGGT CATGGGGCTG GCCTGGACCG CAATGGGAGG CTCCACGCTG TTTGTGGAGA 
     3181 CATCCCTGAG ACGGCCACAG GACAAGGATG CCAAGGGTGA CAAGGATGGC AGCCTGGAGG 
     3241 TGACAGGCCA GCTGGGGGAG GTGATGAAGG AGAGCGCCCG CATAGCCTAC ACCTTCGCCA 
     3301 GAGCCTTCCT CATGCAGCAC GCCCCCGCCA ATGACTACCT GGTGACCTCA CACATCCACC 
     3361 TGCATGTGCC CGAGGGCGCC ACCCCCAAGG ACGGCCCAAG CGCAGGCTGC ACCATCGTCA 
     3421 CGGCCCTGCT GTCCCTGGCC ATGGGCAGGC CTGTCCGGCA GAATCTGGCC ATGACTGGCG 
     3481 AAGTCTCCCT CACGGGCAAG ATCCTGCCTG TTGGTGGCAT CAAGGAGAAG ACCATTGCGG 
     3541 CCAAGCGCGC AGGGGTGACG TGCATCGTCC TGCCAGCCGA GAACAAGAAG GACTTCTACG 
     3601 ACCTGGCAGC CTTCATCACC GAGGGCCTGG AGGTGCACTT CGTGGAACAC TACCGGGAGA 
     3661 TCTTCGACAT CGCCTTCCCG GACGAGCAGG CAGAGGCGCT GGCCGTGGAA CGGACGCGTA 
     3721 CGCGGCCGCT CGAGATGGAG AGCGACGAGA GCGGCCTGCC CGCCATGGAG ATCGAGTGCC 
     3781 GCATCACCGG CACCCTGAAC GGCGTGGAGT TCGAGCTGGT GGGCGGCGGA GAGGGCACCC 
     3841 CCGAGCAGGG CCGCATGACC AACAAGATGA AGAGCACCAA AGGCGCCCTG ACCTTCAGCC 
     3901 CCTACCTGCT GAGCCACGTG ATGGGCTACG GCTTCTACCA CTTCGGCACC TACCCCAGCG 
     3961 GCTACGAGAA CCCCTTCCTG CACGCCATCA ACAACGGCGG CTACACCAAC ACCCGCATCG 
     4021 AGAAGTACGA GGACGGCGGC GTGCTGCACG TGAGCTTCAG CTACCGCTAC GAGGCCGGCC 
     4081 GCGTGATCGG CGACTTCAAG GTGATGGGCA CCGGCTTCCC CGAGGACAGC GTGATCTTCA 
     4141 CCGACAAGAT CATCCGCAGC AACGCCACCG TGGAGCACCT GCACCCCATG GGCGATAACG 
     4201 ATCTGGATGG CAGCTTCACC CGCACCTTCA GCCTGCGCGA CGGCGGCTAC TACAGCTCCG 
     4261 TGGTGGACAG CCACATGCAC TTCAAGAGCG CCATCCACCC CAGCATCCTG CAGAACGGGG 
     4321 GCCCCATGTT CGCCTTCCGC CGCGTGGAGG AGGATCACAG CAACACCGAG CTGGGCATCG 
     4381 TGGAGTACCA GCACGCCTTC AAGACCCCGG ATGCAGATGC CGGTGAAGAA AGAGTTTAAA 
     4441 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4501 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4561 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4621 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4681 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4741 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4801 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4861 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4921 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4981 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5041 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5101 TTGGGTACGC GGCCAGCTTG GCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA 
     5161 GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT 
     5221 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA 
     5281 GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC 
     5341 CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCG 
     5401 GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA 
     5461 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG 
     5521 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG 
     5581 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG 
     5641 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT 
     5701 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA 
     5761 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG 
     5821 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT 
     5881 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA 
     5941 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG 
     6001 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG 
     6061 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG 
     6121 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC CGACCGCTAT 
     6181 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC 
     6241 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC 
     6301 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC 
     6361 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG 
     6421 GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT 
     6481 TCTTCGCCCA CCCCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA 
     6541 TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC 
     6601 TCATCAATGT ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT 
     6661 CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC 
     6721 GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA 
     6781 TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT 
     6841 GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC 
     6901 TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG 
     6961 CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG 
     7021 GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC 
     7081 GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG 
     7141 GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA 
     7201 CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC 
     7261 ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG 
     7321 TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT 
     7381 CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA 
     7441 GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA 
     7501 CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG 
     7561 TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTTTTTTT GTTTGCAAGC 
     7621 AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT 
     7681 CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA 
     7741 GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT 
     7801 ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA 
     7861 TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC 
     7921 GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG 
     7981 CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG 
     8041 CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT 
     8101 CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT 
     8161 CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT 
     8221 CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA 
     8281 AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA 
     8341 TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT 
     8401 AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC 
     8461 ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA 
     8521 GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT 
     8581 CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG 
     8641 CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT 
     8701 ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT 
     8761 AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC 
     8821 CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC 
     8881 TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG 
     8941 CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT 
     9001 TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC 
     9061 CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT 
     9121 TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA 
     9181 TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA 
     9241 ATTTT
//