LOCUS       RG233152                9164 bp    DNA     circular  SYN 
DEFINITION  PLA2G4E (tGFP-tagged) - Homo sapiens phospholipase A2, group IVE (PLA2G4E)
ACCESSION   NM_001206670
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3645..4349 
                     /label="GFP"  
     misc_feature    4396..4981 
                     /label="PolyA signal"  
     misc_feature    7073..7718 
                     /label="ColE1"  
     misc_feature    5435..6229 
                     /label="Neo"  
     misc_feature    5066..5368 
                     /label="SV40 ori"  
     misc_feature    7733..8593 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3624..3630 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3624 
                     /label="PLA2G4E"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGTCTCCAG GCCTCGGAAG GCTGTCCTGG CCTGGGAACT AATGTGTTTG 
     1081 TCCCACAGAG CCCACAAACG GATGAAGAAG GCAGCAGGTC AGGAAGAAGT TTCAGTGAGT 
     1141 TCGAGGATAC ACAGGACCTG GACACTCCTG GTCTCCCACC TTTCTGTCCT ATGGCTCCTT 
     1201 GGGGCTCTGA GGAGGGGCTG TCTCCATGCC ACCTGTTGAC AGTGAGGGTC ATCCGGATGA 
     1261 AAAATGTCCG GCAGGCTGAT ATGCTGAGCC AGACAGACTG TTTTGTGAGC CTCTGGCTGC 
     1321 CCACCGCCTC TCAGAAGAAG CTGAGGACAA GGACCATCTC CAACTGCCCA AATCCAGAGT 
     1381 GGAATGAAAG CTTCAACTTC CAGATCCAGA GCCGAGTGAA GAACGTGCTA GAGTTGAGTG 
     1441 TCTGTGATGA AGACACAGTG ACACCAGATG ACCATCTCCT GACAGTTCTC TATGACCTCA 
     1501 CCAAGCTCTG TTTCCGAAAG AAAACCCACG TGAAGTTTCC ACTCAACCCG CAGGGCATGG 
     1561 AAGAGCTGGA GGTGGAGTTC CTGCTGGAGG AGAGTCCCTC TCCACCTGAG ACCCTCGTCA 
     1621 CCAATGGCGT GCTGGTGTCT CGACAAGTCT CCTGCCTGGA GGTTCATGCA CAATCCAGGA 
     1681 GGCGGAGGAA GAGGGAGAAA ATGAAGGACC TCCTGGTGAT GGTGAACGAA TCCTTTGAGA 
     1741 ACACCCAGCG TGTCCGGCCC TGCTTGGAAC CCTGCTGCCC AACCTCTGCC TGCTTCCAAA 
     1801 CCGCTGCCTG CTTCCACTAC CCCAAGTACT TCCAGTCCCA GGTGCACGTG GAAGTGCCCA 
     1861 AGAGTCACTG GAGCTGTGGG CTTTGCTGCC GCTCTCGCAA GAAGGGCCCC ATCAGCCAGC 
     1921 CCCTCGACTG CCTTTCCGAT GGTCAGGTGA TGACCCTGCC TGTGGGTGAG AGTTATGAAT 
     1981 TACACATGAA GTCTACACCC TGCCCTGAGA CACTGGACGT GCGGCTGGGC TTCAGCCTGT 
     2041 GCCCAGCAGA GCTGGAGTTT CTGCAGAAGC GGAAGGTCGT GGTGGCCAAG GCCCTGAAGC 
     2101 AGGTGCTGCA GCTGGAGGAA GACCTGCAGG AGGACGAGGT GCCGCTGATA GCCATCATGG 
     2161 CCACTGGGGG TGGAACAAGA TCCATGACCT CCATGTATGG CCACCTGCTG GGGCTGCAGA 
     2221 AGCTGAACCT CCTGGACTGT GCCAGCTACA TCACCGGTCT ATCAGGGGCC ACCTGGACCA 
     2281 TGGCTACCTT GTACCGTGAC CCTGACTGGT CCTCCAAAAA CTTGGAGCCT GCTATCTTTG 
     2341 AGGCTCGGAG ACATGTGGTA AAGGACAAGC TACCCTCCCT GTTCCCAGAC CAGCTCCGCA 
     2401 AATTCCAGGA GGAGCTCCGG CAGCGCAGCC AGGAAGGCTA CAGGGTCACC TTTACAGACT 
     2461 TCTGGGGCCT GCTGATAGAG ACCTGCCTGG GGGACGAGAG AAATGAATGC AAACTGTCAG 
     2521 ATCAGCGTGC TGCTTTGAGC TGCGGCCAGA ACCCCCTGCC CATCTACCTC ACCATCAATG 
     2581 TCAAGGATGA TGTAAGCAAC CAGGACTTCA GAGAGTGGTT CGAGTTCTCC CCCTACGAGG 
     2641 TGGGCCTGCA GAAGTATGGG GCCTTCATCC CCTCCGAGCT CTTCGGCTCC GAGTTCTTCA 
     2701 TGGGGCGGCT GGTGAAGAGG ATCCCGGAGT CTCGAATCTG CTACATGCTA GGCCTGTGGA 
     2761 GCAGCATCTT CTCCCTGAAC CTGCTGGATG CCTGGAACCT GTCACACACC TCGGAGGAGT 
     2821 TTTTCCACAG GTGGACAAGG GAGAAAGTGC AGGACATCGA AGACGAGCCG ATCCTGCCTG 
     2881 AAATCCCCAA ATGTGATGCT AACATCCTGG AGACCACGGT AGTGATCCCA GGGTCATGGC 
     2941 TGTCCAATTC TTTCCGAGAA ATCCTTACCC ATCGGTCCTT CGTGTCTGAG TTTCACAACT 
     3001 TCCTGTCTGG GCTGCAGCTG CACACCAACT ACCTCCAGAA TGGCCAGTTC TCTAGGTGGA 
     3061 AAGACACAGT GCTAGATGGT TTCCCAAACC AGCTGACCGA GTCCGCGAAC CACCTGTGCC 
     3121 TGCTGGACAC TGCGTTCTTT GTCAACTCCA GCTACCCGCC CCTCCTCAGG CCAGAGCGAA 
     3181 AAGCCGACCT CATCATCCAC CTCAACTACT GTGCTGGGTC CCAGACAAAG CCCCTGAAAC 
     3241 AAACCTGTGA GTACTGCACT GTGCAGAACA TCCCCTTCCC CAAATACGAG CTGCCAGATG 
     3301 AGAATGAAAA TCTCAAGGAA TGCTACCTGA TGGAGAACCC CCAGGAACCC GATGCCCCCA 
     3361 TCGTGACTTT CTTCCCACTC ATCAATGACA CTTTCCGAAA ATACAAGGCA CCAGGTGTAG 
     3421 AGCGAAGCCC TGAGGAGCTG GAGCAGGGCC AGGTGGACAT TTATGGTCCC AAAACTCCCT 
     3481 ATGCCACCAA GGAGCTGACA TACACAGAGG CCACCTTTGA CAAGCTGGTG AAACTCTCAG 
     3541 AGTATAACAT CCTGAATAAT AAGGACACTC TCCTCCAGGC TCTGCGGCTC GCAGTGGAGA 
     3601 AGAAGAAGCG CCTGAAGGGC CAGTGTCCCT CCACGCGTAC GCGGCCGCTC GAGATGGAGA 
     3661 GCGACGAGAG CGGCCTGCCC GCCATGGAGA TCGAGTGCCG CATCACCGGC ACCCTGAACG 
     3721 GCGTGGAGTT CGAGCTGGTG GGCGGCGGAG AGGGCACCCC CGAGCAGGGC CGCATGACCA 
     3781 ACAAGATGAA GAGCACCAAA GGCGCCCTGA CCTTCAGCCC CTACCTGCTG AGCCACGTGA 
     3841 TGGGCTACGG CTTCTACCAC TTCGGCACCT ACCCCAGCGG CTACGAGAAC CCCTTCCTGC 
     3901 ACGCCATCAA CAACGGCGGC TACACCAACA CCCGCATCGA GAAGTACGAG GACGGCGGCG 
     3961 TGCTGCACGT GAGCTTCAGC TACCGCTACG AGGCCGGCCG CGTGATCGGC GACTTCAAGG 
     4021 TGATGGGCAC CGGCTTCCCC GAGGACAGCG TGATCTTCAC CGACAAGATC ATCCGCAGCA 
     4081 ACGCCACCGT GGAGCACCTG CACCCCATGG GCGATAACGA TCTGGATGGC AGCTTCACCC 
     4141 GCACCTTCAG CCTGCGCGAC GGCGGCTACT ACAGCTCCGT GGTGGACAGC CACATGCACT 
     4201 TCAAGAGCGC CATCCACCCC AGCATCCTGC AGAACGGGGG CCCCATGTTC GCCTTCCGCC 
     4261 GCGTGGAGGA GGATCACAGC AACACCGAGC TGGGCATCGT GGAGTACCAG CACGCCTTCA 
     4321 AGACCCCGGA TGCAGATGCC GGTGAAGAAA GAGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4381 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4441 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4501 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4561 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4621 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4681 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4741 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4801 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4861 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     4921 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     4981 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCTTGG 
     5041 CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 
     5101 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA 
     5161 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA 
     5221 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA 
     5281 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG 
     5341 TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA 
     5401 TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT 
     5461 GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA 
     5521 CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG 
     5581 GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG 
     5641 CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT 
     5701 GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT 
     5761 CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG 
     5821 CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT 
     5881 ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC 
     5941 GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC 
     6001 GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA 
     6061 TTCATCGACT GTGGCCGGCT GGGTGTGGCC GACCGCTATC AGGACATAGC GTTGGCTACC 
     6121 CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT 
     6181 ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA 
     6241 GCGGGACTCT GGGGTTCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT 
     6301 TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG 
     6361 GCTGGATGAT CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT 
     6421 TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG 
     6481 CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG 
     6541 TCTGTATACC GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG 
     6601 TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA 
     6661 AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG 
     6721 CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA 
     6781 GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG 
     6841 TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG 
     6901 AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC 
     6961 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA 
     7021 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT 
     7081 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC 
     7141 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC 
     7201 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC 
     7261 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT 
     7321 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG 
     7381 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA 
     7441 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA 
     7501 AACAAACCAC CGCTGGTAGC GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA 
     7561 AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA 
     7621 ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT 
     7681 TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA 
     7741 GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA 
     7801 TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC 
     7861 CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA 
     7921 ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC 
     7981 AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA 
     8041 ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT 
     8101 TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG 
     8161 CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC 
     8221 TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT 
     8281 CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT 
     8341 GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC 
     8401 TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT 
     8461 CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA 
     8521 GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA 
     8581 CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG 
     8641 GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG 
     8701 TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG 
     8761 CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG 
     8821 CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC 
     8881 TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA 
     8941 AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC 
     9001 CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC 
     9061 TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT 
     9121 GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//