LOCUS       RG231501                8785 bp    DNA     circular  SYN 
DEFINITION  PEG10 (tGFP-tagged) - Human paternally expressed 10 (PEG10), transcript variant 1
ACCESSION   NM_001184961
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3266..3970 
                     /label="GFP"  
     misc_feature    4017..4602 
                     /label="PolyA signal"  
     misc_feature    6694..7339 
                     /label="ColE1"  
     misc_feature    5056..5850 
                     /label="Neo"  
     misc_feature    4687..4989 
                     /label="SV40 ori"  
     misc_feature    7354..8214 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3245..3251 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3245 
                     /label="PEG10"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCCT GGGTCCCGAC TGCCCACCTC CTCCTCCTCC CCCTCCCCCC AACAACAACA 
     1081 ACAACAACAA CTCCAAGCAC ACCGGCCATA AGAGTGCGTG TGTCCCCAAC ATGACCGAAC 
     1141 GAAGAAGGGA CGAGCTCTCT GAAGAGATCA ACAACTTAAG AGAGAAGGTC ATGAAGCAGT 
     1201 CGGAGGAGAA CAACAACCTG CAGAGCCAGG TGCAGAAGCT CACAGAGGAG AACACCACCC 
     1261 TTCGAGAGCA AGTGGAACCC ACCCCTGAGG ATGAGGATGA TGACATCGAG CTCCGCGGTG 
     1321 CTGCAGCAGC TGCTGCCCCA CCCCCTCCAA TAGAGGAAGA GTGCCCAGAA GACCTCCCAG 
     1381 AGAAGTTCGA TGGCAACCCA GACATGCTGG CTCCTTTCAT GGCCCAGTGC CAGATCTTCA 
     1441 TGGAAAAGAG CACCAGGGAT TTCTCAGTTG ATCGTGTCCG TGTCTGCTTC GTGACAAGCA 
     1501 TGATGACCGG CCGTGCTGCC CGTTGGGCCT CAGCAAAGCT GGAGCGCTCC CACTACCTGA 
     1561 TGCACAACTA CCCAGCTTTC ATGATGGAAA TGAAGCATGT CTTTGAAGAC CCTCAGAGGC 
     1621 GAGAGGTTGC CAAACGCAAG ATCAGACGCC TGCGCCAAGG CATGGGGTCT GTCATCGACT 
     1681 ACTCCAATGC TTTCCAGATG ATTGCCCAGG ACCTGGATTG GAACGAGCCT GCGCTGATTG 
     1741 ACCAGTACCA CGAGGGCCTC AGCGACCACA TTCAGGAGGA GCTCTCCCAC CTCGAGGTCG 
     1801 CCAAGTCGCT GTCTGCTCTG ATTGGGCAGT GCATTCACAT TGAGAGAAGG CTGGCCAGGG 
     1861 CTGCTGCAGC TCGCAAGCCA CGCTCGCCAC CCCGGGCGCT GGTGTTGCCT CACATTGCAA 
     1921 GCCACCACCA GGTAGATCCA ACCGAGCCGG TGGGAGGTGC CCGCATGCGC CTGACGCAGG 
     1981 AAGAAAAAGA AAGACGCAGA AAGCTGAACC TGTGCCTCTA CTGTGGAACA GGAGGTCACT 
     2041 ACGCTGACAA TTGTCCTGCC AAGGCCTCAA AGTCTTCGCC GGCGGGAAAC TCCCCGGCCC 
     2101 CGCTGTAGAG GGACCTTCAG CGACCGGGCC AGAAATAATA AGGTCCCCAC AAGATGATGC 
     2161 CTCATCTCCA CACTTGCAAG TGATGCTCCA GATTCATCTT CCGGGCAGAC ACACCCTGTT 
     2221 CGTCCGAGCC ATGATCGATT CTGGTGCTTC TGGCAACTTC ATTGATCACG AATATGTTGC 
     2281 TCAAAATGGA ATTCCTCTAA GAATCAAGGA CTGGCCAATA CTTGTGGAAG CAATTGATGG 
     2341 GCGCCCCATA GCATCGGGCC CAGTTGTCCA CGAAACTCAC GACCTGATAG TTGACCTGGG 
     2401 AGATCACCGA GAGGTGCTGT CATTTGATGT GACTCAGTCT CCATTCTTCC CTGTCGTCCT 
     2461 AGGGGTTCGC TGGCTGAGCA CACATGATCC CAATATCACA TGGAGCACTC GATCTATCGT 
     2521 CTTTGATTCT GAATACTGCC GCTACCACTG CCGGATGTAT TCTCCAATAC CACCATCGCT 
     2581 CCCACCACCA GCACCACAAC CGCCACTCTA TTATCCAGTA GATGGATACA GAGTTTACCA 
     2641 ACCAGTGAGG TATTACTATG TCCAGAATGT GTACACTCCA GTAGATGAGC ACGTCTACCC 
     2701 AGATCACCGC CTGGTTGACC CTCACATAGA AATGATACCT GGAGCACACA GTATTCCCAG 
     2761 TGGACATGTG TATTCACTGT CCGAACCTGA AATGGCAGCT CTTCGAGATT TTGTGGCAAG 
     2821 AAATGTAAAA GATGGGCTAA TTACTCCAAC GATTGCACCT AATGGAGCCC AAGTTCTCCA 
     2881 GGTGAAGAGG GGGTGGAAAC TGCAAGTTTC TTATGATTGC CGAGCTCCAA ACAATTTTAC 
     2941 TATCCAGAAT CAGTATCCTC GCCTATCTAT TCCAAATTTA GAAGACCAAG CACACCTGGC 
     3001 AACGTACACT GAATTCGTAC CTCAAATACC TGGATACCAA ACATACCCCA CATATGCCGC 
     3061 GTACCCGACC TACCCAGTAG GATTCGCCTG GTACCCAGTG GGACGAGACG GACAAGGAAG 
     3121 ATCACTATAT GTACCTGTGA TGATCACTTG GAATCCACAC TGGTACCGCC AGCCTCCGGT 
     3181 ACCACAGTAC CCGCCGCCAC AGCCGCCGCC TCCACCACCA CCACCGCCGC CGCCTCCATC 
     3241 TTACAGTACC CTGACGCGTA CGCGGCCGCT CGAGATGGAG AGCGACGAGA GCGGCCTGCC 
     3301 CGCCATGGAG ATCGAGTGCC GCATCACCGG CACCCTGAAC GGCGTGGAGT TCGAGCTGGT 
     3361 GGGCGGCGGA GAGGGCACCC CCGAGCAGGG CCGCATGACC AACAAGATGA AGAGCACCAA 
     3421 AGGCGCCCTG ACCTTCAGCC CCTACCTGCT GAGCCACGTG ATGGGCTACG GCTTCTACCA 
     3481 CTTCGGCACC TACCCCAGCG GCTACGAGAA CCCCTTCCTG CACGCCATCA ACAACGGCGG 
     3541 CTACACCAAC ACCCGCATCG AGAAGTACGA GGACGGCGGC GTGCTGCACG TGAGCTTCAG 
     3601 CTACCGCTAC GAGGCCGGCC GCGTGATCGG CGACTTCAAG GTGATGGGCA CCGGCTTCCC 
     3661 CGAGGACAGC GTGATCTTCA CCGACAAGAT CATCCGCAGC AACGCCACCG TGGAGCACCT 
     3721 GCACCCCATG GGCGATAACG ATCTGGATGG CAGCTTCACC CGCACCTTCA GCCTGCGCGA 
     3781 CGGCGGCTAC TACAGCTCCG TGGTGGACAG CCACATGCAC TTCAAGAGCG CCATCCACCC 
     3841 CAGCATCCTG CAGAACGGGG GCCCCATGTT CGCCTTCCGC CGCGTGGAGG AGGATCACAG 
     3901 CAACACCGAG CTGGGCATCG TGGAGTACCA GCACGCCTTC AAGACCCCGG ATGCAGATGC 
     3961 CGGTGAAGAA AGAGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG 
     4021 GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG 
     4081 TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT 
     4141 TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA 
     4201 CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC 
     4261 TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT 
     4321 TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG 
     4381 GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT 
     4441 GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA 
     4501 TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG 
     4561 CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA 
     4621 CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCTTG GCTGTGGAAT GTGTGTCAGT 
     4681 TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA 
     4741 ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA 
     4801 GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC 
     4861 TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG 
     4921 CAGAGGCCGA GGCCGCCTCG GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG 
     4981 GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC 
     5041 AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC 
     5101 CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT 
     5161 CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG 
     5221 ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA 
     5281 CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC 
     5341 TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA 
     5401 AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC 
     5461 CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC 
     5521 TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG 
     5581 CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT 
     5641 GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC 
     5701 TGGGTGTGGC CGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC 
     5761 TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC 
     5821 AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA 
     5881 AATGACCGAC CAAGCGACGC CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT 
     5941 CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG 
     6001 CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAACTTG TTTATTGCAG CTTATAATGG 
     6061 TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC 
     6121 TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGTATAC CGTCGACCTC 
     6181 TAGCTAGAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT 
     6241 CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG 
     6301 AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT 
     6361 GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG 
     6421 GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC 
     6481 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     6541 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     6601 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     6661 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     6721 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     6781 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     6841 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     6901 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     6961 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     7021 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     7081 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     7141 CGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     7201 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     7261 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     7321 TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG 
     7381 TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT 
     7441 CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC 
     7501 GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC 
     7561 CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG 
     7621 GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC 
     7681 AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG 
     7741 ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC 
     7801 TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT 
     7861 GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC 
     7921 AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT 
     7981 ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC 
     8041 TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC 
     8101 TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA 
     8161 AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT 
     8221 CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG 
     8281 ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG 
     8341 AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC 
     8401 GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC 
     8461 TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT 
     8521 AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG 
     8581 TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC 
     8641 GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA 
     8701 TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT 
     8761 TTAACAAAAA TTTAACGCGA ATTTT
//