LOCUS       RG231375                8621 bp    DNA     circular  SYN 
DEFINITION  PDE4D (tGFP-tagged) - Human phosphodiesterase 4D, cAMP-specific (PDE4D), transcript variant 5
ACCESSION   NM_001197219
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3102..3806 
                     /label="GFP"  
     misc_feature    3853..4438 
                     /label="PolyA signal"  
     misc_feature    6530..7175 
                     /label="ColE1"  
     misc_feature    4892..5686 
                     /label="Neo"  
     misc_feature    4523..4825 
                     /label="SV40 ori"  
     misc_feature    7190..8050 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3081..3087 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3081 
                     /label="PDE4D"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTTTTGTT TGGGATCCTC TGGGAGCCAC GGTGCCAGGA CCATCTACAA 
     1081 GAGCCAAATC AAGATTGCGT TTCTCAAAGT CCTACAGTTT TGATGTGGAC AATGGCACAT 
     1141 CTGCGGGACG GAGTCCCTTG GATCCCATGA CCAGCCCAGG ATCCGGGCTA ATTCTCCAAG 
     1201 CAAATTTTGT CCACAGTCAA CGACGGGAGT CCTTCCTGTA TCGATCCGAC AGCGATTATG 
     1261 ACCTCTCTCC AAAGTCTATG TCCCGGAACT CCTCCATTGC CAGTGATATA CACGGAGATG 
     1321 ACTTGATTGT GACTCCATTT GCTCAGGTCT TGGCCAGTCT GCGAACTGTA CGAAACAACT 
     1381 TTGCTGCATT AACTAATTTG CAAGATCGAG CACCTAGCAA AAGATCACCC ATGTGCAACC 
     1441 AACCATCCAT CAACAAAGCC ACCATAACAG AGGAGGCCTA CCAGAAACTG GCCAGCGAGA 
     1501 CCCTGGAGGA GCTGGACTGG TGTCTGGACC AGCTAGAGAC CCTACAGACC AGGCACTCCG 
     1561 TCAGTGAGAT GGCCTCCAAC AAGTTTAAAA GGATGCTTAA TCGGGAGCTC ACCCATCTCT 
     1621 CTGAAATGAG TCGGTCTGGA AATCAAGTGT CAGAGTTTAT ATCAAACACA TTCTTAGATA 
     1681 AGCAACATGA AGTGGAAATT CCTTCTCCAA CTCAGAAGGA AAAGGAGAAA AAGAAAAGAC 
     1741 CAATGTCTCA GATCAGTGGA GTCAAGAAAT TGATGCACAG CTCTAGTCTG ACTAATTCAA 
     1801 GTATCCCAAG GTTTGGAGTT AAAACTGAAC AAGAAGATGT CCTTGCCAAG GAACTAGAAG 
     1861 ATGTGAACAA ATGGGGTCTT CATGTTTTCA GAATAGCAGA GTTGTCTGGT AACCGGCCCT 
     1921 TGACTGTTAT CATGCACACC ATTTTTCAGG AACGGGATTT ATTAAAAACA TTTAAAATTC 
     1981 CAGTAGATAC TTTAATTACA TATCTTATGA CTCTCGAAGA CCATTACCAT GCTGATGTGG 
     2041 CCTATCACAA CAATATCCAT GCTGCAGATG TTGTCCAGTC TACTCATGTG CTATTATCTA 
     2101 CACCTGCTTT GGAGGCTGTG TTTACAGATT TGGAGATTCT TGCAGCAATT TTTGCCAGTG 
     2161 CAATACATGA TGTAGATCAT CCTGGTGTGT CCAATCAATT TCTGATCAAT ACAAACTCTG 
     2221 AACTTGCCTT GATGTACAAT GATTCCTCAG TCTTAGAGAA CCATCATTTG GCTGTGGGCT 
     2281 TTAAATTGCT TCAGGAAGAA AACTGTGACA TTTTCCAGAA TTTGACCAAA AAACAAAGAC 
     2341 AATCTTTAAG GAAAATGGTC ATTGACATCG TACTTGCAAC AGATATGTCA AAACACATGA 
     2401 ATCTACTGGC TGATTTGAAG ACTATGGTTG AAACTAAGAA AGTGACAAGC TCTGGAGTTC 
     2461 TTCTTCTTGA TAATTATTCC GATAGGATTC AGGTTCTTCA GAATATGGTG CACTGTGCAG 
     2521 ATCTGAGCAA CCCAACAAAG CCTCTCCAGC TGTACCGCCA GTGGACGGAC CGGATAATGG 
     2581 AGGAGTTCTT CCGCCAAGGA GACCGAGAGA GGGAACGTGG CATGGAGATA AGCCCCATGT 
     2641 GTGACAAGCA CAATGCTTCC GTGGAAAAAT CACAGGTGGG CTTCATAGAC TATATTGTTC 
     2701 ATCCCCTCTG GGAGACATGG GCAGACCTCG TCCACCCTGA CGCCCAGGAT ATTTTGGACA 
     2761 CTTTGGAGGA CAATCGTGAA TGGTACCAGA GCACAATCCC TCAGAGCCCC TCTCCTGCAC 
     2821 CTGATGACCC AGAGGAGGGC CGGCAGGGTC AAACTGAGAA ATTCCAGTTT GAACTAACTT 
     2881 TAGAGGAAGA TGGTGAGTCA GACACGGAAA AGGACAGTGG CAGTCAAGTG GAAGAAGACA 
     2941 CTAGCTGCAG TGACTCCAAG ACTCTTTGTA CTCAAGACTC AGAGTCTACT GAAATTCCCC 
     3001 TTGATGAACA GGTTGAAGAG GAGGCAGTAG GGGAAGAAGA GGAAAGCCAG CCTGAAGCCT 
     3061 GTGTCATAGA TGATCGTTCT CCTGACACGA CGCGTACGCG GCCGCTCGAG ATGGAGAGCG 
     3121 ACGAGAGCGG CCTGCCCGCC ATGGAGATCG AGTGCCGCAT CACCGGCACC CTGAACGGCG 
     3181 TGGAGTTCGA GCTGGTGGGC GGCGGAGAGG GCACCCCCGA GCAGGGCCGC ATGACCAACA 
     3241 AGATGAAGAG CACCAAAGGC GCCCTGACCT TCAGCCCCTA CCTGCTGAGC CACGTGATGG 
     3301 GCTACGGCTT CTACCACTTC GGCACCTACC CCAGCGGCTA CGAGAACCCC TTCCTGCACG 
     3361 CCATCAACAA CGGCGGCTAC ACCAACACCC GCATCGAGAA GTACGAGGAC GGCGGCGTGC 
     3421 TGCACGTGAG CTTCAGCTAC CGCTACGAGG CCGGCCGCGT GATCGGCGAC TTCAAGGTGA 
     3481 TGGGCACCGG CTTCCCCGAG GACAGCGTGA TCTTCACCGA CAAGATCATC CGCAGCAACG 
     3541 CCACCGTGGA GCACCTGCAC CCCATGGGCG ATAACGATCT GGATGGCAGC TTCACCCGCA 
     3601 CCTTCAGCCT GCGCGACGGC GGCTACTACA GCTCCGTGGT GGACAGCCAC ATGCACTTCA 
     3661 AGAGCGCCAT CCACCCCAGC ATCCTGCAGA ACGGGGGCCC CATGTTCGCC TTCCGCCGCG 
     3721 TGGAGGAGGA TCACAGCAAC ACCGAGCTGG GCATCGTGGA GTACCAGCAC GCCTTCAAGA 
     3781 CCCCGGATGC AGATGCCGGT GAAGAAAGAG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     3841 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     3901 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     3961 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4021 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4081 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4141 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     4201 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     4261 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     4321 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     4381 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     4441 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCTTGGCTG 
     4501 TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG 
     4561 CAAAGCATGC ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA 
     4621 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT 
     4681 CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA 
     4741 ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCGGCCT CTGAGCTATT CCAGAAGTAG 
     4801 TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC 
     4861 ATTTTCGGAT CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA 
     4921 TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA 
     4981 CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT 
     5041 CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG 
     5101 CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA 
     5161 GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC 
     5221 CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT 
     5281 GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT 
     5341 CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG 
     5401 CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG 
     5461 ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC 
     5521 ATCGACTGTG GCCGGCTGGG TGTGGCCGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT 
     5581 GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT TCCTCGTGCT TTACGGTATC 
     5641 GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAGCG 
     5701 GGACTCTGGG GTTCGAAATG ACCGACCAAG CGACGCCCAA CCTGCCATCA CGAGATTTCG 
     5761 ATTCCACCGC CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT 
     5821 GGATGATCCT CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCC AACTTGTTTA 
     5881 TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT 
     5941 TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT 
     6001 GTATACCGTC GACCTCTAGC TAGAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT 
     6061 GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG 
     6121 CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT 
     6181 TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG 
     6241 GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG 
     6301 TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT 
     6361 CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA 
     6421 AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA 
     6481 ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC 
     6541 CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT 
     6601 CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA 
     6661 GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG 
     6721 ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT 
     6781 CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA 
     6841 CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT 
     6901 GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC 
     6961 AAACCACCGC TGGTAGCGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG 
     7021 GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT 
     7081 CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA 
     7141 ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT 
     7201 ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG 
     7261 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA 
     7321 GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC 
     7381 AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT 
     7441 CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG 
     7501 TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA 
     7561 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG 
     7621 TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA 
     7681 TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG 
     7741 TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT 
     7801 CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA 
     7861 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA 
     7921 GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG 
     7981 TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC 
     8041 GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT 
     8101 ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC 
     8161 CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG 
     8221 CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC 
     8281 CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA 
     8341 ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC 
     8401 TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT 
     8461 TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA 
     8521 ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT 
     8581 TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//