LOCUS       RG230885                9470 bp    DNA     circular  SYN 
DEFINITION  PLK4 (tGFP-tagged) - Human polo-like kinase 4 (PLK4), transcript variant 3
ACCESSION   NM_001190801
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3951..4655 
                     /label="GFP"  
     misc_feature    4702..5287 
                     /label="PolyA signal"  
     misc_feature    7379..8024 
                     /label="ColE1"  
     misc_feature    5741..6535 
                     /label="Neo"  
     misc_feature    5372..5674 
                     /label="SV40 ori"  
     misc_feature    8039..8899 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3930..3936 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3930 
                     /label="PLK4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGACCTGC ATCGGGGAGA AGATCGAGGA TTTTAAAGTT GGAAATCTGC 
     1081 TTGGTAAAGG ATCATTTGCT GGTGTCTACA GAGCTGAGTC CATTCACACT GGTTTGGAAG 
     1141 TTGCAATCAA AATGATAGAT AAGAAAGCCA TGTACAAAGC AGGAATGGTA CAGAGAGTCC 
     1201 AAAATGAGGT GAAAATACAT TGCCAATTGA AACATCCTTC TATCTTGGAG CTTTATAACT 
     1261 ATTTTGAAGA TAGCAATTAT GTGTATCTGG TATTAGAAAT GTGCCATAAT GGAGAAATGA 
     1321 ACAGGTATCT AAAGAATAGA GTGAAACCCT TCTCAGAAAA TGAAGCTCGA CACTTCATGC 
     1381 ACCAGATCAT CACAGGGATG TTGTATCTTC ATTCTCATGG TATACTACAC CGGGACCTCA 
     1441 CACTTTCTAA CCTCCTACTG ACTCGTAATA TGAACATCAA GATTGCTGAT TTTGGGCTGG 
     1501 CAACTCAACT GAAAATGCCA CATGAAAAGC ACTATACATT ATGTGGAACT CCTAACTACA 
     1561 TTTCACCAGA AATTGCCACT CGAAGTGCAC ATGGCCTTGA ATCTGATGTT TGGTCCCTGG 
     1621 GCTGTATGTT TTATACATTA CTTATCGGGA GACCACCCTT CGACACTGAC ACAGTCAAGA 
     1681 ACACATTAAA TAAAGTAGTA TTGGCAGATT ATGAAATGCC ATCTTTTTTG TCAATAGAGG 
     1741 CCAAGGACCT TATTCACCAG TTACTTCGTA GAAATCCAGC AGATCGTTTA AGTCTGTCTT 
     1801 CAGTATTGGA CCATCCTTTT ATGTCCCGAA ATTCTTCAAC AAAAAGTAAA GATTTAGGAA 
     1861 CTGTGGAAGA CTCAATTGAT AGTGGGCATG CCACAATTTC TACTGCAATT ACAGCTTCTT 
     1921 CCAGTACCAG TATAAGTGGT AGTTTATTTG ACAAAAGAAG ACTTTTGATT GGTCAGCCAC 
     1981 TCCCAAATAA AATGACTGTA TTTCCAAAGA ATAAAAGTTC AACTGATTTT TCTTCTTCAG 
     2041 GAGATGGAAA CAGTTTTTAT ACTCAGTGGG GAAATCAAGA AACCAGTAAT AGTGGAAGGG 
     2101 GAAGAGTAAT TCAAGATGCA GAAGAAAGGC CACATTCTCG ATACCTTCGT AGAGCTTATT 
     2161 CCTCTGATAG ATCTGGCACT TCTAATAGTC AGTCTCAAGC AAAAACATAT ACAATGGAAC 
     2221 GATGTCACTC AGCAGAAATG CTTTCAGTGT CCAAAAGATC AGGAGGAGGT GAAAATGAAG 
     2281 AGAGGTACTC ACCCACAGAC AACAATGCCA ACATTTTTAA CTTCTTTAAA GAAAAGACAT 
     2341 CCAGTAGTTC TGGATCTTTT GAAAGACCTG ATAACAATCA AGCACTCTCC AATCATCTTT 
     2401 GTCCAGGAAA AACTCCTTTT CCATTTGCAG ACCCGACACC TCAGACTGAA ACCGTACAAC 
     2461 AGTGGTTTGG GAATCTGCAA ATAAATGCTC ATTTAAGAAA AACTACTGAA TATGACAGCA 
     2521 TCAGCCCAAA CCGGGACTTC CAGGGCCATC CAGATTTGCA GAAGGACACA TCAAAAAATG 
     2581 CCTGGACTGA TACAAAAGTC AAAAAGAACT CTGATGCTTC TGATAATGCA CATTCTGTAA 
     2641 AACAGCAAAA TACCATGAAA TATATGACTG CACTTCACAG TAAACCTGAG ATAATCCAAC 
     2701 AAGAATGTGT TTTTGGCTCA GATCCTCTTT CTGAACAGAG CAAGACTAGG GGTATGGAGC 
     2761 CACCATGGGG TTATCAGAAT CGTACATTAA GAAGCATTAC ATCTCCGTTG GTTGCTCACA 
     2821 GGTTAAAACC AATCAGACAG AAAACCAAAA AGGCTGTGGT GAGCATACTT GATTCAGAGG 
     2881 AGGTGTGTGT GGAGCTTGTA AAGGAGTATG CATCTCAAGA ATATGTGAAA GAAGTTCTTC 
     2941 AGATATCTAG TGATGGAAAT ACGATCACTA TTTATTATCC AAATGGTGGT AGAGGTTTTC 
     3001 CTCTTGCTGA TAGACCACCC TCACCTACTG ACAACATCAG TAGGTACAGC TTTGACAATT 
     3061 TACCAGAAAA ATACTGGCGA AAATATCAAT ATGCTTCCAG GTTTGTACAG CTTGTAAGAT 
     3121 CTAAATCTCC CAAAATCACT TATTTTACAA GATATGCTAA ATGCATTTTG ATGGAGAATT 
     3181 CTCCTGGTGC TGATTTTGAG GTTTGGTTTT ATGATGGGGT AAAAATACAC AAAACAGAAG 
     3241 ATTTCATTCA GGTGATTGAA AAGACAGGGA AGTCTTACAC TTTAAAAAGT GAAAGTGAAG 
     3301 TTAATAGCTT GAAAGAGGAG ATAAAAATGT ATATGGACCA TGCTAATGAG GGTCATCGTA 
     3361 TTTGTTTAGC ACTGGAATCC ATAATTTCAG AAGAGGAAAG GAAAACTAGG AGTGCTCCCT 
     3421 TTTTCCCAAT AATCATAGGA AGAAAACCTG GTAGTACTAG TTCACCTAAG GCCTTATCAC 
     3481 CTCCTCCTTC TGTGGATTCA AATTACCCAA CGAGAGAGAG AGCATCTTTC AACAGAATGG 
     3541 TCATGCATAG TGCTGCTTCT CCAACACAGG CACCAATCCT TAATCCCTCT ATGGTTACAA 
     3601 ATGAAGGACT TGGTCTTACA ACTACAGCTT CTGGAACAGA CATCTCTTCT AATAGTCTAA 
     3661 AAGATTGTCT TCCTAAATCA GCACAACTTT TGAAATCTGT TTTTGTGAAA AATGTTGGTT 
     3721 GGGCTACACA GTTAACTAGT GGAGCTGTGT GGGTTCAGTT TAATGATGGG TCCCAGTTGG 
     3781 TTGTGCAGGC AGGAGTGTCT TCTATCAGTT ATACCTCACC AAATGGTCAA ACAACTAGGT 
     3841 ATGGAGAAAA TGAAAAATTA CCAGACTACA TCAAACAGAA ATTACAGTGT CTGTCTTCCA 
     3901 TCCTTTTGAT GTTTTCTAAT CCGACTCCTA ATTTTCATAC GCGTACGCGG CCGCTCGAGA 
     3961 TGGAGAGCGA CGAGAGCGGC CTGCCCGCCA TGGAGATCGA GTGCCGCATC ACCGGCACCC 
     4021 TGAACGGCGT GGAGTTCGAG CTGGTGGGCG GCGGAGAGGG CACCCCCGAG CAGGGCCGCA 
     4081 TGACCAACAA GATGAAGAGC ACCAAAGGCG CCCTGACCTT CAGCCCCTAC CTGCTGAGCC 
     4141 ACGTGATGGG CTACGGCTTC TACCACTTCG GCACCTACCC CAGCGGCTAC GAGAACCCCT 
     4201 TCCTGCACGC CATCAACAAC GGCGGCTACA CCAACACCCG CATCGAGAAG TACGAGGACG 
     4261 GCGGCGTGCT GCACGTGAGC TTCAGCTACC GCTACGAGGC CGGCCGCGTG ATCGGCGACT 
     4321 TCAAGGTGAT GGGCACCGGC TTCCCCGAGG ACAGCGTGAT CTTCACCGAC AAGATCATCC 
     4381 GCAGCAACGC CACCGTGGAG CACCTGCACC CCATGGGCGA TAACGATCTG GATGGCAGCT 
     4441 TCACCCGCAC CTTCAGCCTG CGCGACGGCG GCTACTACAG CTCCGTGGTG GACAGCCACA 
     4501 TGCACTTCAA GAGCGCCATC CACCCCAGCA TCCTGCAGAA CGGGGGCCCC ATGTTCGCCT 
     4561 TCCGCCGCGT GGAGGAGGAT CACAGCAACA CCGAGCTGGG CATCGTGGAG TACCAGCACG 
     4621 CCTTCAAGAC CCCGGATGCA GATGCCGGTG AAGAAAGAGT TTAAACGGCC GGCCGCGGTC 
     4681 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4741 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4801 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4861 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4921 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4981 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5041 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5101 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5161 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5221 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5281 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5341 GCTTGGCTGT GGAATGTGTG TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC 
     5401 AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC 
     5461 TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG 
     5521 CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT 
     5581 GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCGGCCTC TGAGCTATTC 
     5641 CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT 
     5701 TGTATATCCA TTTTCGGATC TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA 
     5761 CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC 
     5821 TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG 
     5881 CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG 
     5941 GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT 
     6001 GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG 
     6061 TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG 
     6121 CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA 
     6181 GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG 
     6241 GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT 
     6301 CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT 
     6361 TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCCGACC GCTATCAGGA CATAGCGTTG 
     6421 GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT 
     6481 TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC 
     6541 TTCTGAGCGG GACTCTGGGG TTCGAAATGA CCGACCAAGC GACGCCCAAC CTGCCATCAC 
     6601 GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTCCGGG 
     6661 ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT GGAGTTCTTC GCCCACCCCA 
     6721 ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA 
     6781 ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT 
     6841 ATCATGTCTG TATACCGTCG ACCTCTAGCT AGAGCTTGGC GTAATCATGG TCATAGCTGT 
     6901 TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA 
     6961 AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC 
     7021 TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG 
     7081 CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC 
     7141 GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT 
     7201 CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA 
     7261 GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC 
     7321 ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC 
     7381 AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG 
     7441 GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA 
     7501 GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG 
     7561 TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC 
     7621 ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG 
     7681 GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT 
     7741 TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT 
     7801 CCGGCAAACA AACCACCGCT GGTAGCGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA 
     7861 GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA 
     7921 ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA 
     7981 TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT 
     8041 CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT 
     8101 CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT 
     8161 CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG 
     8221 CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT 
     8281 CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT 
     8341 TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG 
     8401 CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA 
     8461 AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT 
     8521 TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT 
     8581 GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC 
     8641 CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA 
     8701 AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT 
     8761 TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT 
     8821 TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA 
     8881 GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT 
     8941 ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA 
     9001 TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT 
     9061 TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG 
     9121 CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC 
     9181 AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC 
     9241 CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT 
     9301 TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA 
     9361 CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG 
     9421 CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//