LOCUS       RG230878                8972 bp    DNA     circular  SYN 
DEFINITION  RASAL1 (GFP-tagged) - Human RAS protein activator like 1 (GAP1 like) (RASAL1), transcript variant 1
ACCESSION   NM_001193520
VERSION     NM_001193520.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3453..4157 
                     /label="GFP"  
     misc_feature    4204..4789 
                     /label="PolyA signal"  
     misc_feature    6881..7526 
                     /label="ColE1"  
     misc_feature    5243..6037 
                     /label="Neo"  
     misc_feature    4874..5176 
                     /label="SV40 ori"  
     misc_feature    7541..8401 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3432..3438 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3432 
                     /label="RASAL1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCAAGAGC AGCTCCCTGA ATGTTCGCGT GGTGGAGGGC CGCGCGCTGC 
     1081 CTGCCAAGGA CGTGTCTGGG AGCAGCGACC CCTACTGCCT AGTGAAAGTG GACGACGAGG 
     1141 TGGTGGCCAG GACAGCTACT GTCTGGAGGA GCCTGGGCCC CTTCTGGGGG GAGGAGTACA 
     1201 CGGTGCACCT GCCTCTGGAT TTCCACCAGC TGGCCTTCTA CGTGCTGGAT GAGGACACTG 
     1261 TCGGGCACGA CGACATCATC GGCAAGATCT CGCTGAGCAG GGAGGCGATT ACAGCCGACC 
     1321 CCCGAGGGAT TGACAGCTGG ATTAACTTGA GCCGAGTGGA CCCAGATGCA GAAGTGCAGG 
     1381 GTGAGATCTG CCTGTCAGTG CAGATGCTGG AGGATGGGCA GGGCCGCTGC CTTCGCTGCC 
     1441 ATGTGCTTCA GGCCAGGGAC CTGGCTCCCA GAGACATCTC TGGCACATCT GACCCATTTG 
     1501 CACGTGTGTT TTGGGGCAGC CAGAGCTTGG AGACCTCAAC CATCAAGAAG ACTCGCTTCC 
     1561 CGCACTGGGA TGAAGTGCTG GAGCTGCGGG AGATGCCAGG TGCCCCGTCC CCACTGCGGG 
     1621 TGGAGCTCTG GGACTGGGAC ATGGTGGGCA AGAATGACTT CTTGGGCATG GTGGAGTTCT 
     1681 CTCCAAAGAC CCTCCAGCAG AAGCCACCTA AAGGCTGGTT CCGCCTCCTG CCCTTTCCCA 
     1741 GAGCCGAGGA GGATTCTGGG GGGAACCTGG GTGCCCTGCG AGTGAAGGTA CGCCTGATTG 
     1801 AGGACCGCGT CCTGCCCTCC CAGTGCTACC AGCCTCTCAT GGAGCTGCTC ATGGAGTCTG 
     1861 TGCAGGGGCC AGCAGAGGAG GACACTGCTA GCCCCTTGGC TTTGCTGGAA GAGCTGACCT 
     1921 TGGGGGACTG CCGCCAGGAC CTTGCCACCA AGCTGGTGAA ACTCTTTCTT GGCCGGGGAC 
     1981 TGGCTGGGCA CTTTCTGGAC TATCTCACCC GGCGTGAGGT GGCTCGGACC ATGGACCCCA 
     2041 ACACCCTCTT CCGTTCTAAC TCCCTGGCAT CCAAGTCGAT GGAACAGTTT ATGAAGCTCG 
     2101 TGGGCATGCC CTACCTGCAC GAGGTCCTGA AGCCTGTGAT TAGCCGTGTC TTTGAGGAGA 
     2161 AGAAGTACAT GGAGCTGGAT CCCTGCAAGA TGGACCTGGG CCGCACCCGG AGGATCTCCT 
     2221 TCAAAGGCGC ACTCTCGGAG GAGCAGATGC GGGAGACCAG CCTGGGGCTG CTGACGGGCT 
     2281 ACCTGGGGCC CATCGTGGAC GCCATCGTGG GCTCCGTGGG GCGCTGCCCG CCCGCCATGC 
     2341 GCCTCGCCTT CAAGCAGCTG CACCGGCGAG TGGAGGAGCG CTTCCCCCAG GCCGAGCACC 
     2401 AGGATGTGAA GTACCTGGCC ATCAGTGGAT TTCTCTTCTT GCGATTCTTC GCACCTGCCA 
     2461 TCCTTACCCC AAAGCTGTTT GACCTTCGGG ACCAACACGC GGACCCCCAG ACTAGCCGCT 
     2521 CACTGCTGTT GCTTGCCAAG GCTGTGCAGA GCATTGGAAA CCTGGGCCAG CAGCTGGGCC 
     2581 AAGGCAAGGA ACTGTGGATG GCCCCCCTGC ACCCCTTCCT GCTGCAGTGT GTCTCACGTG 
     2641 TGAGAGACTT CCTGGACCGG CTGGTGGATG TGGATGGGGA TGAAGCTGGT GTCCCAGCCA 
     2701 GGGCCCTGTT CCCGCCCTCG GCCATTGTTC GAGAAGGCTA TCTGCTGAAG CGCAAGGAGG 
     2761 AGCCTGCCGG CCTGGCCACG CGCTTTGCCT TCAAGAAGCG CTACGTCTGG CTCAGCGGGG 
     2821 AGACCCTCTC CTTCTCCAAG AGTCCTGAGT GGCAGATGTG TCACTCCATC CCCGTGTCTC 
     2881 ACATCCGCGC CGTGGAGCGC GTAGACGAGG GCGCCTTCCA ACTGCCCCAC GTGATGCAGG 
     2941 TGGTGACGCA GGACGGCACG GGGGCGCTGC ACACCACCTA CCTCCAGTGC AAGAATGTGA 
     3001 ATGAGCTCAA CCAGTGGCTC TCGGCCTTGC GCAAGGCCAG CGCCCCCAAC CCGAACAAGC 
     3061 TGGCCGCCTG CCACCCCGGT GCCTTCCGCA GCGCGCGCTG GACCTGCTGC CTCCAGGCTG 
     3121 AGCGCTCAGC CGCCGGCTGC AGCCGTACAC ACTCAGCTGT CACCCTGGGG GACTGGAGTG 
     3181 ACCCACTGGA TCCTGATGCT GAGGCCCAGA CAGTGTATCG GCAGCTGCTC CTGGGGCGGG 
     3241 ACCAGCTCAG GCTGAAATTA CTGGAGGATT CTAACATGGA TACAACTCTG GAGGCAGACA 
     3301 CAGGGGCCTG TCCTGAGGTC CTGGCCCGGC AAAGAGCAGC AACTGCCCGC CTGCTGGAGG 
     3361 TGCTCGCAGA CCTGGATCGT GCCCACGAGG AGTTCCAGCA GCAGGAGCGA GGGAAGGCGG 
     3421 CCCTGGGCCC CCTTGGCCCC ACGCGTACGC GGCCGCTCGA GATGGAGAGC GACGAGAGCG 
     3481 GCCTGCCCGC CATGGAGATC GAGTGCCGCA TCACCGGCAC CCTGAACGGC GTGGAGTTCG 
     3541 AGCTGGTGGG CGGCGGAGAG GGCACCCCCG AGCAGGGCCG CATGACCAAC AAGATGAAGA 
     3601 GCACCAAAGG CGCCCTGACC TTCAGCCCCT ACCTGCTGAG CCACGTGATG GGCTACGGCT 
     3661 TCTACCACTT CGGCACCTAC CCCAGCGGCT ACGAGAACCC CTTCCTGCAC GCCATCAACA 
     3721 ACGGCGGCTA CACCAACACC CGCATCGAGA AGTACGAGGA CGGCGGCGTG CTGCACGTGA 
     3781 GCTTCAGCTA CCGCTACGAG GCCGGCCGCG TGATCGGCGA CTTCAAGGTG ATGGGCACCG 
     3841 GCTTCCCCGA GGACAGCGTG ATCTTCACCG ACAAGATCAT CCGCAGCAAC GCCACCGTGG 
     3901 AGCACCTGCA CCCCATGGGC GATAACGATC TGGATGGCAG CTTCACCCGC ACCTTCAGCC 
     3961 TGCGCGACGG CGGCTACTAC AGCTCCGTGG TGGACAGCCA CATGCACTTC AAGAGCGCCA 
     4021 TCCACCCCAG CATCCTGCAG AACGGGGGCC CCATGTTCGC CTTCCGCCGC GTGGAGGAGG 
     4081 ATCACAGCAA CACCGAGCTG GGCATCGTGG AGTACCAGCA CGCCTTCAAG ACCCCGGATG 
     4141 CAGATGCCGG TGAAGAAAGA GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4201 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4261 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4321 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4381 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4441 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4501 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4561 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4621 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4681 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     4741 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     4801 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCTTGGCT GTGGAATGTG 
     4861 TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 
     4921 CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 
     4981 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC 
     5041 CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT 
     5101 ATTTATGCAG AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC 
     5161 TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA 
     5221 TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA 
     5281 GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC 
     5341 GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC 
     5401 AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG 
     5461 CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG 
     5521 GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT 
     5581 GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT 
     5641 ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA 
     5701 GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA 
     5761 CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC 
     5821 GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT 
     5881 GGCCGGCTGG GTGTGGCCGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT 
     5941 GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC 
     6001 GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG 
     6061 GGTTCGAAAT GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG 
     6121 CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC 
     6181 TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT 
     6241 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC 
     6301 TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT 
     6361 CGACCTCTAG CTAGAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT 
     6421 ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG 
     6481 CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG 
     6541 GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC 
     6601 GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC 
     6661 GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA 
     6721 ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG 
     6781 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT 
     6841 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA 
     6901 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC 
     6961 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT 
     7021 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG 
     7081 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG 
     7141 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT 
     7201 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC 
     7261 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG 
     7321 CTGGTAGCGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG 
     7381 AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG 
     7441 GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT 
     7501 GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT 
     7561 TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC 
     7621 TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA 
     7681 TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG 
     7741 GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT 
     7801 GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA 
     7861 TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT 
     7921 CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT 
     7981 TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG 
     8041 CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG 
     8101 AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG 
     8161 CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA 
     8221 AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT 
     8281 AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT 
     8341 GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT 
     8401 GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA 
     8461 TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT 
     8521 TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG 
     8581 TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT 
     8641 TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC 
     8701 TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG 
     8761 GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG 
     8821 AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT 
     8881 CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG 
     8941 AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//