LOCUS       RG230613                9572 bp    DNA     circular  SYN 
DEFINITION  CNKSR2 (tGFP-tagged) - Human connector enhancer of kinase suppressor of Ras 2 (CNKSR2), transcript variant 2
ACCESSION   NM_001168647
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4053..4757 
                     /label="GFP"  
     misc_feature    4804..5389 
                     /label="PolyA signal"  
     misc_feature    7481..8126 
                     /label="ColE1"  
     misc_feature    5843..6637 
                     /label="Neo"  
     misc_feature    5474..5776 
                     /label="SV40 ori"  
     misc_feature    8141..9001 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4032..4038 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4032 
                     /label="CNKSR2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTCTGATA ATGGAACCGG TGAGCAAATG GTCTCCGAGT CAAGTAGTGG 
     1081 ACTGGATGAA AGGTCTTGAT GACTGTTTGC AGCAGTATAT TAAGAACTTT GAGAGGGAGA 
     1141 AGATCAGTGG GGACCAGCTG CTGCGCATTA CACATCAGGA GCTAGAAGAT CTGGGGGTCA 
     1201 GCCGCATTGG CCATCAGGAA CTGATCTTGG AAGCAGTTGA CCTTCTGTGT GCATTGAATT 
     1261 ATGGCTTGGA AACAGAAAAT CTAAAAACCC TTTCTCACAA GTTGAATGCA TCTGCCAAAA 
     1321 ATCTGCAGAA TTTTATAACA GGAAGGAGAA GGAGTGGCCA TTATGATGGG AGGACCAGCC 
     1381 GAAAATTGCC AAACGACTTT CTGACCTCAG TTGTGGATCT GATTGGAGCA GCCAAGAGTC 
     1441 TGCTTGCCTG GTTGGACAGG TCACCATTTG CTGCTGTGAC AGACTATTCA GTTACAAGAA 
     1501 ATAATGTCAT ACAACTCTGC CTGGAGTTAA CAACAATTGT GCAACAGGAT TGTACTGTAT 
     1561 ATGAAACAGA GAATAAAATT CTTCACGTGT GTAAAACTCT TTCTGGAGTC TGTGACCACA 
     1621 TCATATCCCT GTCGTCAGAT CCTCTGGTTT CACAGTCTGC TCACCTGGAA GTGATTCAAC 
     1681 TGGCAAACAT TAAACCAAGC GAAGGGCTGG GTATGTATAT TAAATCTACA TATGATGGCC 
     1741 TCCATGTAAT TACTGGAACC ACAGAAAATT CACCTGCAGA TCGGTGCAAG AAAATCCATG 
     1801 CTGGCGATGA AGTGATTCAA GTTAATCATC AGACTGTGGT GGGGTGGCAG TTGAAAAATT 
     1861 TGGTGAATGC ACTACGAGAG GACCCGAGTG GTGTTATCTT AACTTTGAAA AAGCGACCTC 
     1921 AGAGCATGCT TACCTCAGCA CCAGCTTTAC TGAAAAATAT GAGATGGAAG CCCCTTGCTC 
     1981 TGCAGCCTCT TATACCTAGA AGTCCCACAA GCAGCGTTGC CACGCCTTCC AGCACCATCA 
     2041 GTACACCCAC CAAAAGAGAC AGTTCTGCCC TCCAGGATCT CTACATTCCC CCTCCTCCTG 
     2101 CAGAACCATA TATTCCCAGG GATGAAAAAG GAAACCTTCC TTGTGAAGAC CTCAGAGGAC 
     2161 ATATGGTGGG CAAGCCAGTG CATAAGGGAT CTGAATCACC AAATTCATTT CTGGATCAGG 
     2221 AATATCGAAA GAGATTTAAT ATTGTCGAAG AAGATACTGT CTTATATTGC TATGAATATG 
     2281 AAAAAGGAAG ATCAAGTAGT CAAGGAAGAC GAGAAAGCAC CCCAACTTAT GAAAACTCTC 
     2341 TACTTCGGTA TATGAGCAAT GAAAAGATTG CTCAAGAAGA ATACATGTTT CAGAGAAACA 
     2401 GCAAAAAGGA CACAGGGAAG AAGTCAAAAA AGAAGGGTGA TAAGAGTAAT AGCCCAACTC 
     2461 ACTATTCATT GCTACCTAGT TTACAAATGG ATGCACTGAG ACAAGACATC ATGGGCACTC 
     2521 CTGTGCCAGA GACCACACTA TACCATACAT TTCAGCAGTC CTCACTGCAG CACAAATCAA 
     2581 AGAAGAAAAA CAAAGGTCCT ATAGCAGGCA AGAGCAAAAG ACGAATTTCT TGCAAAGATC 
     2641 TTGGCCGTGG TGACTGTGAG GGCTGGCTTT GGAAAAAGAA AGATGCGAAG AGTTACTTTT 
     2701 CACAGAAATG GAAAAAATAT TGGTTTGTCC TAAAGGATGC ATCCCTTTAT TGGTATATTA 
     2761 ATGAGGAGGA TGAAAAAGCA GAAGGATTCA TTAGCCTGCC TGAATTTAAA ATTGATAGAG 
     2821 CCAGTGAATG CCGCAAAAAA TATGCATTCA AAGCCTGTCA TCCTAAAATC AAAAGCTTTT 
     2881 ATTTTGCTGC TGAACATCTT GATGATATGA ACAGGTGGCT TAACAGAATT AATATGCTGA 
     2941 CTGCAGGATA TGCAGAAAGA GAGAGGATTA AGCAGGAACA AGATTACTGG AGTGAGAGTG 
     3001 ACAAGGAAGA AGCAGATACT CCATCAACAC CAAAACAAGA TAGCCCTCCA CCCCCATATG 
     3061 ATACATACCC ACGACCTCCC TCGATGAGTT GCGCCAGTCC TTATGTGGAA GCAAAACATA 
     3121 GCCGACTTTC CTCCACGGAG ACTTCTCAGT CTCAGTCTTC TCATGAGGAG TTTCGCCAGG 
     3181 AAGTAACTGG GAGCAGTGCA GTGTCTCCCA TTCGCAAGAC AGCCAGTCAG CGCCGCTCCT 
     3241 GGCAGGATTT AATTGAGACG CCACTGACAA GTTCAGGCTT ACACTATCTT CAGACTCTGC 
     3301 CCCTGGAGGA TTCTGTCTTC TCTGACTCCG CGGCCATCTC CCCAGAGCAC AGGCGGCAGT 
     3361 CTACCCTGCC AACTCAGAAA TGCCACCTGC AGGATCACTA TGGGCCATAC CCCTTAGCTG 
     3421 AGAGTGAGAG GATGCAAGTG CTAAATGGAA ATGGGGGCAA GCCTCGAAGT TTTACTCTGC 
     3481 CTCGAGATAG CGGGTTCAAC CATTGCTGTC TGAATGCTCC AGTTAGTGCC TGTGACCCAC 
     3541 AGGATGACGT GCAACCCCCA GAGGTGGAGG AAGAGGAGGA GGAGGAGGAG GAGGAAGGGG 
     3601 AGGCAGCAGG GGAAAACATA GGAGAAAAAA GTGAAAGCAG AGAAGAAAAG TTAGGAGACT 
     3661 CATTGCAAGA TTTATACAGG GCACTGGAGC AGGCCAGTCT GTCACCACTA GGAGAACATC 
     3721 GTATTTCAAC CAAGATGGAA TACAAGCTAT CATTTATAAA AAGATGTAAT GATCCTGTAA 
     3781 TGAATGAAAA ACTACACCGG CTGAGAATTC TCAAAAGCAC TTTAAAGGCC AGAGAAGGGG 
     3841 AAGTAGCCAT TATCGATAAA GTCCTAGACA ATCCAGACTT GACATCTAAA GAATTCCAAC 
     3901 AATGGAAGCA GATGTACCTC GACCTTTTCT TGGATATCTG TCAAAATACC ACCTCAAATG 
     3961 ACCCACTGAG TATTTCTTCT GAAGTAGATG TAATCACTTC CTCTCTAGCA CACACTCATT 
     4021 CATACATTGA AACGCATGTC ACGCGTACGC GGCCGCTCGA GATGGAGAGC GACGAGAGCG 
     4081 GCCTGCCCGC CATGGAGATC GAGTGCCGCA TCACCGGCAC CCTGAACGGC GTGGAGTTCG 
     4141 AGCTGGTGGG CGGCGGAGAG GGCACCCCCG AGCAGGGCCG CATGACCAAC AAGATGAAGA 
     4201 GCACCAAAGG CGCCCTGACC TTCAGCCCCT ACCTGCTGAG CCACGTGATG GGCTACGGCT 
     4261 TCTACCACTT CGGCACCTAC CCCAGCGGCT ACGAGAACCC CTTCCTGCAC GCCATCAACA 
     4321 ACGGCGGCTA CACCAACACC CGCATCGAGA AGTACGAGGA CGGCGGCGTG CTGCACGTGA 
     4381 GCTTCAGCTA CCGCTACGAG GCCGGCCGCG TGATCGGCGA CTTCAAGGTG ATGGGCACCG 
     4441 GCTTCCCCGA GGACAGCGTG ATCTTCACCG ACAAGATCAT CCGCAGCAAC GCCACCGTGG 
     4501 AGCACCTGCA CCCCATGGGC GATAACGATC TGGATGGCAG CTTCACCCGC ACCTTCAGCC 
     4561 TGCGCGACGG CGGCTACTAC AGCTCCGTGG TGGACAGCCA CATGCACTTC AAGAGCGCCA 
     4621 TCCACCCCAG CATCCTGCAG AACGGGGGCC CCATGTTCGC CTTCCGCCGC GTGGAGGAGG 
     4681 ATCACAGCAA CACCGAGCTG GGCATCGTGG AGTACCAGCA CGCCTTCAAG ACCCCGGATG 
     4741 CAGATGCCGG TGAAGAAAGA GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4801 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4861 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4921 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4981 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     5041 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     5101 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     5161 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5221 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5281 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5341 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5401 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCTTGGCT GTGGAATGTG 
     5461 TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 
     5521 CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 
     5581 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC 
     5641 CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT 
     5701 ATTTATGCAG AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC 
     5761 TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA 
     5821 TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA 
     5881 GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC 
     5941 GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC 
     6001 AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG 
     6061 CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG 
     6121 GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT 
     6181 GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT 
     6241 ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA 
     6301 GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA 
     6361 CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC 
     6421 GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT 
     6481 GGCCGGCTGG GTGTGGCCGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT 
     6541 GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC 
     6601 GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG 
     6661 GGTTCGAAAT GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG 
     6721 CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC 
     6781 TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT 
     6841 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC 
     6901 TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT 
     6961 CGACCTCTAG CTAGAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT 
     7021 ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG 
     7081 CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG 
     7141 GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC 
     7201 GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC 
     7261 GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA 
     7321 ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG 
     7381 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT 
     7441 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA 
     7501 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC 
     7561 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT 
     7621 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG 
     7681 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG 
     7741 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT 
     7801 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC 
     7861 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG 
     7921 CTGGTAGCGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG 
     7981 AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG 
     8041 GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT 
     8101 GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT 
     8161 TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC 
     8221 TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA 
     8281 TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG 
     8341 GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT 
     8401 GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA 
     8461 TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT 
     8521 CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT 
     8581 TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG 
     8641 CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG 
     8701 AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG 
     8761 CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA 
     8821 AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT 
     8881 AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT 
     8941 GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT 
     9001 GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA 
     9061 TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT 
     9121 TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG 
     9181 TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT 
     9241 TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC 
     9301 TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG 
     9361 GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG 
     9421 AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT 
     9481 CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG 
     9541 AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//