LOCUS       RG230551                9167 bp    DNA     circular  SYN 
DEFINITION  CLCN2 (tGFP-tagged) - Human chloride channel 2 (CLCN2), transcript variant 4
ACCESSION   NM_001171089
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3648..4352 
                     /label="GFP"  
     misc_feature    4399..4984 
                     /label="PolyA signal"  
     misc_feature    7076..7721 
                     /label="ColE1"  
     misc_feature    5438..6232 
                     /label="Neo"  
     misc_feature    5069..5371 
                     /label="SV40 ori"  
     misc_feature    7736..8596 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3627..3633 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3627 
                     /label="CLCN2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCCGCG GCGGCGGAGG AAGGGATGGA GCCACGGGCG CTGCAGTACG 
     1081 AGCAGACCCT GATGTATGGC CGGTACACTC AGGACCTTGG GGCCTTTGCC AAAGAGGAAG 
     1141 CTGCTCGGAT TCGCCTGGGA GGGCCTGAAC CCTGGAAAGG TCCCCCTTCC TCTCGGGCTG 
     1201 CCCCAGAGCT CTTGGAATAT GGACGGAGCC GTTGCGCCCG ATGCCGCGTC TGTTCTGTCC 
     1261 GCTGCCACAA GTTCCTAGTA TCCAGGGTTG GTGAAGATTG GATCTTCCTG GTCCTGCTGG 
     1321 GGCTTCTCAT GGCATTGGTC AGCTGGGTCA TGGACTATGC CATTGCTGCC TGTCTGCAAG 
     1381 CCCAGCAGTG GATGTCCCGG GGCTTGAACA CCAGCATCTT GCTCCAGTAC CTGGCCTGGG 
     1441 TCACCTACCC TGTTGTCCTC ATCACTTTCT CAGCCGGATT CACACAGATC CTGGCCCCTC 
     1501 AGGCTGTCGG CTCTGGCATC CCTGAGATGA AGACCATCTT GCGGGGAGTG GTGCTGAAAG 
     1561 AATACCTCAC ACTCAAGACC TTTATAGCTA AGGTCATTGG GCTGACCTGC GCCCTAGGCA 
     1621 GCGGGATGCC GCTTGGCAAA GAGGGCCCTT TTGTGCATAT CGCAAGCATG TGTGCTGCCC 
     1681 TTCTCAGCAA GTTCCTCTCC CTCTTTGGGG GTATCTATGA GAATGAATCC CGGAACACAG 
     1741 AGATGCTGGC TGCCGCCTGT GCCGTGGGGG TGGGCTGCTG CTTCGCGGCA CCTATTGGAG 
     1801 GCGTCCTCTT CAGCATCGAG GTCACCTCCA CCTTCTTTGC AGTGCGGAAC TACTGGCGGG 
     1861 GCTTCTTCGC TGCCACCTTC AGTGCCTTCA TCTTCCGGGT CTTGGCAGTC TGGAACCGGG 
     1921 ATGAAGAGAC TATTACAGCC CTCTTCAAAA CCCGATTCCG GCTCGACTTC CCCTTTGACC 
     1981 TGCAGGAGCT GCCAGCCTTT GCTGTCATTG GTATTGCTAG TGGCTTCGGT GGAGCCCTCT 
     2041 TTGTCTACCT GAACCGGAAG ATTGTCCAGG TGATGCGGAA GCAGAAAACC ATCAATCGCT 
     2101 TCCTCATGAG GAAACGCCTG CTCTTCCCGG CTCTGGTGAC CCTGCTCATC TCCACGCTGA 
     2161 CCTTCCCCCC TGGCTTTGGA CAGTTCATGG CTGGACAGCT CTCACAGAAA GAGACGCTGG 
     2221 TCACCCTGTT TGACAATCGG ACGTGGGTCC GCCAGGGCCT GGTGGAGGAG CTAGAACCAC 
     2281 CCAGCACCTC ACAGGCCTGG AACCCACCAC GTGCCAACGT CTTCCTCACC CTGGTCATCT 
     2341 TCATTCTCAT GAAGTTCTGG ATGTCTGCAC TGGCCACCAC CATCCCAGTT CCCTGTGGGG 
     2401 CCTTCATGCC TGTCTTTGTC ATTGGAGCAG CATTTGGGCG TCTGGTGGGT GAAAGCATGG 
     2461 CTGCCTGGTT CCCAGATGGA ATTCATACGG ACAGCAGCAC CTACCGGATT GTGCCTGGGG 
     2521 GCTACGCTGT GGTCGGGGCA GCTGCGCTGG CAGGAGCGGT GACACACACA GTGTCCACGG 
     2581 CTGTGATCGT GTTCGAGCTC ACAGGCCAGA TTGCCCACAT CCTGCCTGTC ATGATCGCCG 
     2641 TCATCCTGGC CAACGCTGTC GCCCAGAGTC TGCAGCCCTC CCTCTATGAC AGCATCATCC 
     2701 GAATCAAGAA ACTGCCCTAC CTGCCTGAGC TCGGCTGGGG CCGCCACCAG CAGTACCGGG 
     2761 TGCGTGTGGA GGACATCATG GTGCGGGATG TTCCCCATGT GGCCCTCAGC TGCACCTTCC 
     2821 GGGACCTGCG TTTGGCACTG CACAGGACCA AGGGCCGAAT GCTGGCCCTA GTGGAGTCCC 
     2881 CTGAGTCCAT GATTCTGCTG GGCTCCATCG AGCGTTCACA GGTGGTGGCA TTGTTGGGGG 
     2941 CCCAGCTGAG CCCAGCCCGC CGGCGGCAGC ACATGCAGGA GCGCAGAGCC ACCCAGACCT 
     3001 CTCCACTATC TGATCAGGAG GGTCCCCCTA CCCCTGAGGC TTCTGTCTGC TTCCAGGTGA 
     3061 ACACAGAAGA CTCAGCCTTC CCAGCAGCCC GGGGGGAGAC CCACAAGCCC CTAAAGCCTG 
     3121 CACTCAAGAG GGGGCCCAGT GTCACCAGGA ACCTCGGAGA GAGTCCCACA GGGAGCGCAG 
     3181 AGTCGGCAGG CATCGCCCTC CGGAGCCTCT TCTGTGGCAG TCCACCCCCT GAGGCTGCTT 
     3241 CGGAGAAGTT GGAATCCTGT GAGAAGCGCA AGCTGAAGCG TGTCCGAATC TCCCTGGCAA 
     3301 GTGACGCGGA CCTGGAAGGC GAGATGAGCC CTGAAGAGAT TCTGGAGTGG GAGGAGCAGC 
     3361 AACTAGATGA ACCTGTCAAC TTCAGTGACT GCAAAATTGA TCCTGCTCCC TTCCAGCTGG 
     3421 TGGAGCGGAC CTCTTTGCAC AAGCTCCGGA AGGCCATCGA GGGCTCTGTC ACAGCACAGG 
     3481 GTGTGAAAGT CCGGCCGCCC CTCGCCAGCT TCCGAGACAG TGCCACCAGC AGCAGTGACA 
     3541 CGGAGACCAC TGAGGTGCAT GCACTCTGGG GGCCCCACTC CCGTCATGGC CTCCCCCGGG 
     3601 AGGGCAGCCC TTCCGACAGC GACGACAAAT GCCAAACGCG TACGCGGCCG CTCGAGATGG 
     3661 AGAGCGACGA GAGCGGCCTG CCCGCCATGG AGATCGAGTG CCGCATCACC GGCACCCTGA 
     3721 ACGGCGTGGA GTTCGAGCTG GTGGGCGGCG GAGAGGGCAC CCCCGAGCAG GGCCGCATGA 
     3781 CCAACAAGAT GAAGAGCACC AAAGGCGCCC TGACCTTCAG CCCCTACCTG CTGAGCCACG 
     3841 TGATGGGCTA CGGCTTCTAC CACTTCGGCA CCTACCCCAG CGGCTACGAG AACCCCTTCC 
     3901 TGCACGCCAT CAACAACGGC GGCTACACCA ACACCCGCAT CGAGAAGTAC GAGGACGGCG 
     3961 GCGTGCTGCA CGTGAGCTTC AGCTACCGCT ACGAGGCCGG CCGCGTGATC GGCGACTTCA 
     4021 AGGTGATGGG CACCGGCTTC CCCGAGGACA GCGTGATCTT CACCGACAAG ATCATCCGCA 
     4081 GCAACGCCAC CGTGGAGCAC CTGCACCCCA TGGGCGATAA CGATCTGGAT GGCAGCTTCA 
     4141 CCCGCACCTT CAGCCTGCGC GACGGCGGCT ACTACAGCTC CGTGGTGGAC AGCCACATGC 
     4201 ACTTCAAGAG CGCCATCCAC CCCAGCATCC TGCAGAACGG GGGCCCCATG TTCGCCTTCC 
     4261 GCCGCGTGGA GGAGGATCAC AGCAACACCG AGCTGGGCAT CGTGGAGTAC CAGCACGCCT 
     4321 TCAAGACCCC GGATGCAGAT GCCGGTGAAG AAAGAGTTTA AACGGCCGGC CGCGGTCATA 
     4381 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4441 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4501 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4561 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4621 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4681 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     4741 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     4801 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     4861 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     4921 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     4981 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCT 
     5041 TGGCTGTGGA ATGTGTGTCA GTTAGGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA 
     5101 AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCAGGT GTGGAAAGTC CCCAGGCTCC 
     5161 CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAT AGTCCCGCCC 
     5221 CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC 
     5281 TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CGGCCTCTGA GCTATTCCAG 
     5341 AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT 
     5401 ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA 
     5461 GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG 
     5521 GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC 
     5581 CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA 
     5641 GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC 
     5701 ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA 
     5761 TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT 
     5821 ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA 
     5881 CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG 
     5941 CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGAGGATCTC 
     6001 GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT 
     6061 GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCCGACCGCT ATCAGGACAT AGCGTTGGCT 
     6121 ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC 
     6181 GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC 
     6241 TGAGCGGGAC TCTGGGGTTC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG 
     6301 ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG 
     6361 CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCCAACT 
     6421 TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA 
     6481 AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC 
     6541 ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC 
     6601 CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT 
     6661 GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC 
     6721 CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG 
     6781 GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT 
     6841 CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA 
     6901 CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA 
     6961 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC 
     7021 ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG 
     7081 CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT 
     7141 ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT 
     7201 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC 
     7261 AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG 
     7321 ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG 
     7381 GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG 
     7441 GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG 
     7501 GCAAACAAAC CACCGCTGGT AGCGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA 
     7561 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG 
     7621 AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC 
     7681 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG 
     7741 ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT 
     7801 CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG 
     7861 GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA 
     7921 TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA 
     7981 TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC 
     8041 GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT 
     8101 CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA 
     8161 AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT 
     8221 CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT 
     8281 TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA 
     8341 GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG 
     8401 TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA 
     8461 GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA 
     8521 CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG 
     8581 CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC 
     8641 AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG 
     8701 GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA 
     8761 GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC 
     8821 CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG 
     8881 CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA 
     8941 AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC 
     9001 GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA 
     9061 CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT 
     9121 ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//