LOCUS       RG230540                9107 bp    DNA     circular  SYN 
DEFINITION  CNKSR2 (tGFP-tagged) - Human connector enhancer of kinase suppressor of Ras 2 (CNKSR2), transcript variant 4
ACCESSION   NM_001168649
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3588..4292 
                     /label="GFP"  
     misc_feature    4339..4924 
                     /label="PolyA signal"  
     misc_feature    7016..7661 
                     /label="ColE1"  
     misc_feature    5378..6172 
                     /label="Neo"  
     misc_feature    5009..5311 
                     /label="SV40 ori"  
     misc_feature    7676..8536 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3567..3573 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3567 
                     /label="CNKSR2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTCTGATA ATGGAACCGG TGAGCAAATG GTCTCCGAGT CAAGTAGTGG 
     1081 ACTGGATGAA AGGTCTTGAT GACTGTTTGC AGCAGTATAT TAAGAACTTT GAGAGGGAGA 
     1141 AGATCAGTGG GGACCAGCTG CTGCGCATTA CACATCAGGA GCTAGAAGAT CTGGGGGTCA 
     1201 GCCGCATTGG CCATCAGGAA CTGATCTTGG AAGCAGTTGA CCTTCTGTGT GCATTGAATT 
     1261 ATGGCTTGGA AACAGAAAAT CTAAAAACCC TTTCTCACAA GTTGAATGCA TCTGCCAAAA 
     1321 ATCTGCAGAA TTTTATAACA GGAAGGAGAA GGAGTGGCCA TTATGATGGG AGGACCAGCC 
     1381 GAAAATTGCC AAACGACTTT CTGACCTCAG TTGTGGATCT GATTGGAGCA GCCAAGAGTC 
     1441 TGCTTGCCTG GTTGGACAGG TCACCATTTG CTGCTGTGAC AGACTATTCA GTTACAAGAA 
     1501 ATAATGTCAT ACAACTCTGC CTGGAGTTAA CAACAATTGT GCAACAGGAT TGTACTGTAT 
     1561 ATGAAACAGA GAATAAAATT CTTCACGTGT GTAAAACTCT TTCTGGAGTC TGTGACCACA 
     1621 TCATATCCCT GTCGTCAGAT CCTCTGGTTT CACAGTCTGC TCACCTGGAA GTGATTCAAC 
     1681 TGGCAAACAT TAAACCAAGC GAAGGGCTGG GTATGTATAT TAAATCTACA TATGATGGCC 
     1741 TCCATGTAAT TACTGGAACC ACAGAAAATT CACCTGCAGA TCGGTGCAAG AAAATCCATG 
     1801 CTGGCGATGA AGTGATTCAA GTTAATCATC AGACTGTGCC TCTTATACCT AGAAGTCCCA 
     1861 CAAGCAGCGT TGCCACGCCT TCCAGCACCA TCAGTACACC CACCAAAAGA GACAGTTCTG 
     1921 CCCTCCAGGA TCTCTACATT CCCCCTCCTC CTGCAGAACC ATATATTCCC AGGGATGAAA 
     1981 AAGGAAACCT TCCTTGTGAA GACCTCAGAG GACATATGGT GGGCAAGCCA GTGCATAAGG 
     2041 GATCTGAATC ACCAAATTCA TTTCTGGATC AGGAATATCG AAAGAGATTT AATATTGTCG 
     2101 AAGAAGATAC TGTCTTATAT TGCTATGAAT ATGAAAAAGG AAGATCAAGT AGTCAAGGAA 
     2161 GACGAGAAAG CACCCCAACT TATGGCAAGC TACGACCTAT ATCTATGCCA GTGGAATATA 
     2221 ATTGGGTGGG GGACTATGAA GATCCAAATA AGATGAAGAG AGATAGTAGA AGAGAAAACT 
     2281 CTCTACTTCG GTATATGAGC AATGAAAAGA TTGCTCAAGA AGAATACATG TTTCAGAGAA 
     2341 ACAGCAAAAA GGACACAGGG AAGAAGTCAA AAAAGAAGGG TGATAAGAGT AATAGCCCAA 
     2401 CTCACTATTC ATTGCTACCT AGTTTACAAA TGGATGCACT GAGACAAGAC ATCATGGGCA 
     2461 CTCCTGTGCC AGAGACCACA CTATACCATA CATTTCAGCA GTCCTCACTG CAGCACAAAT 
     2521 CAAAGAAGAA AAACAAAGGT CCTATAGCAG GCAAGAGCAA AAGACGAATT TCTTGCAAAG 
     2581 ATCTTGGCCG TGGTGACTGT GAGGGCTGGC TTTGGAAAAA GAAAGATGCG AAGAGTTACT 
     2641 TTTCACAGAA ATGGAAAAAA TATTGGTTTG TCCTAAAGGA TGCATCCCTT TATTGGTATA 
     2701 TTAATGAGGA GGATGAAAAA GCAGAAGGAT TCATTAGCCT GCCTGAATTT AAAATTGATA 
     2761 GAGCCAGTGA ATGCCGCAAA AAATATGCAT TCAAAGCCTG TCATCCTAAA ATCAAAAGCT 
     2821 TTTATTTTGC TGCTGAACAT CTTGATGATA TGAACAGGTG GCTTAACAGA ATTAATATGC 
     2881 TGACTGCAGG ATATGCAGAA AGAGAGAGGA TTAAGCAGGA ACAAGATTAC TGGAGTGAGA 
     2941 GTGACAAGGA AGAAGCAGAT ACTCCATCAA CACCAAAACA AGATAGCCCT CCACCCCCAT 
     3001 ATGATACATA CCCACGACCT CCCTCGATGA GTTGCGCCAG TCCTTATGTG GAAGCAAAAC 
     3061 ATAGCCGACT TTCCTCCACG GAGACTTCTC AGTCTCAGTC TTCTCATGAG GAGTTTCGCC 
     3121 AGGAAGTAAC TGGGAGCAGT GCAGTGTCTC CCATTCGCAA GACAGCCAGT CAGCGCCGCT 
     3181 CCTGGCAGGA TTTAATTGAG ACGCCACTGA CAAGTTCAGG CTTACACTAT CTTCAGACTC 
     3241 TGCCCCTGGA GGATTCTGTC TTCTCTGACT CCGCGGCCAT CTCCCCAGAG CACAGGCGGC 
     3301 AGTCTACCCT GCCAACTCAG AAATGCCACC TGCAGGATCA CTATGGGCCA TACCCCTTAG 
     3361 CTGAGAGTGA GAGGATGCAA GTGCTAAATG GAAATGGGGG CAAGCCTCGA AGTTTTACTC 
     3421 TGCCTCGAGA TAGCGGGTTC AACCATTGCT GTCTGAATGC TCCAGTTAGT GCCTGTGACC 
     3481 CACAGGATGA CGTGCAACCC CCAGAGGTGG AGGAAGAGGA GGAGGAGGAG GAGGAGGAAG 
     3541 GGGAGGCAGC AGGGGAAAAC ATAGGAGAAA AAAGCACGCG TACGCGGCCG CTCGAGATGG 
     3601 AGAGCGACGA GAGCGGCCTG CCCGCCATGG AGATCGAGTG CCGCATCACC GGCACCCTGA 
     3661 ACGGCGTGGA GTTCGAGCTG GTGGGCGGCG GAGAGGGCAC CCCCGAGCAG GGCCGCATGA 
     3721 CCAACAAGAT GAAGAGCACC AAAGGCGCCC TGACCTTCAG CCCCTACCTG CTGAGCCACG 
     3781 TGATGGGCTA CGGCTTCTAC CACTTCGGCA CCTACCCCAG CGGCTACGAG AACCCCTTCC 
     3841 TGCACGCCAT CAACAACGGC GGCTACACCA ACACCCGCAT CGAGAAGTAC GAGGACGGCG 
     3901 GCGTGCTGCA CGTGAGCTTC AGCTACCGCT ACGAGGCCGG CCGCGTGATC GGCGACTTCA 
     3961 AGGTGATGGG CACCGGCTTC CCCGAGGACA GCGTGATCTT CACCGACAAG ATCATCCGCA 
     4021 GCAACGCCAC CGTGGAGCAC CTGCACCCCA TGGGCGATAA CGATCTGGAT GGCAGCTTCA 
     4081 CCCGCACCTT CAGCCTGCGC GACGGCGGCT ACTACAGCTC CGTGGTGGAC AGCCACATGC 
     4141 ACTTCAAGAG CGCCATCCAC CCCAGCATCC TGCAGAACGG GGGCCCCATG TTCGCCTTCC 
     4201 GCCGCGTGGA GGAGGATCAC AGCAACACCG AGCTGGGCAT CGTGGAGTAC CAGCACGCCT 
     4261 TCAAGACCCC GGATGCAGAT GCCGGTGAAG AAAGAGTTTA AACGGCCGGC CGCGGTCATA 
     4321 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4381 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4441 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4501 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4561 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4621 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     4681 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     4741 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     4801 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     4861 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     4921 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCT 
     4981 TGGCTGTGGA ATGTGTGTCA GTTAGGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA 
     5041 AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCAGGT GTGGAAAGTC CCCAGGCTCC 
     5101 CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAT AGTCCCGCCC 
     5161 CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC 
     5221 TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CGGCCTCTGA GCTATTCCAG 
     5281 AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT 
     5341 ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA 
     5401 GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG 
     5461 GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC 
     5521 CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA 
     5581 GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC 
     5641 ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA 
     5701 TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT 
     5761 ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA 
     5821 CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG 
     5881 CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGAGGATCTC 
     5941 GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT 
     6001 GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCCGACCGCT ATCAGGACAT AGCGTTGGCT 
     6061 ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC 
     6121 GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC 
     6181 TGAGCGGGAC TCTGGGGTTC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG 
     6241 ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG 
     6301 CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCCAACT 
     6361 TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA 
     6421 AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC 
     6481 ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC 
     6541 CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT 
     6601 GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC 
     6661 CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG 
     6721 GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT 
     6781 CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA 
     6841 CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA 
     6901 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC 
     6961 ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG 
     7021 CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT 
     7081 ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT 
     7141 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC 
     7201 AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG 
     7261 ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG 
     7321 GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG 
     7381 GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG 
     7441 GCAAACAAAC CACCGCTGGT AGCGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA 
     7501 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG 
     7561 AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC 
     7621 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG 
     7681 ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT 
     7741 CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG 
     7801 GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA 
     7861 TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA 
     7921 TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC 
     7981 GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT 
     8041 CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA 
     8101 AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT 
     8161 CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT 
     8221 TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA 
     8281 GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG 
     8341 TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA 
     8401 GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA 
     8461 CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG 
     8521 CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC 
     8581 AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG 
     8641 GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA 
     8701 GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC 
     8761 CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG 
     8821 CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA 
     8881 AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC 
     8941 GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA 
     9001 CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT 
     9061 ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//