LOCUS       RG230498                8912 bp    DNA     circular  SYN 
DEFINITION  NRG2 (tGFP-tagged) - Human neuregulin 2 (NRG2), transcript variant 7
ACCESSION   NM_001184935
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3393..4097 
                     /label="GFP"  
     misc_feature    4144..4729 
                     /label="PolyA signal"  
     misc_feature    6821..7466 
                     /label="ColE1"  
     misc_feature    5183..5977 
                     /label="Neo"  
     misc_feature    4814..5116 
                     /label="SV40 ori"  
     misc_feature    7481..8341 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3372..3378 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3372 
                     /label="NRG2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGGCAGGTT TGCTGCTCAG CGCTGCCGCC GCCGCCACTG GAGAAGGGTC 
     1081 GGTGCAGCAG CTACAGCGAC AGCAGCAGCA GCAGCAGCGA GAGGAGCAGC AGCAGCAGCA 
     1141 GCAGCAGCAG CGAGAGCGGC AGCAGCAGCA GGAGCAGCAG CAACAACAGC AGCATCTCTC 
     1201 GTCCCGCTGC GCCCCCAGAG CCGCGGCCGC AGCAACAGCC GCAGCCCCGC AGCCCCGCAG 
     1261 CCCGGAGAGC CGCCGCCCGT TCGCGAGCCG CAGCCGCCGG CGGCATGAGG CGCGACCCGG 
     1321 CCCCCGGCTT CTCCATGCTG CTCTTCGGTG TGTCGCTCGC CTGCTACTCG CCCAGCCTCA 
     1381 AGTCAGTGCA GGACCAGGCG TACAAGGCAC CCGTGGTGGT GGAGGGCAAG GTACAGGGGC 
     1441 TGGTCCCAGC CGGCGGCTCC AGCTCCAACA GCACCCGAGA GCCGCCCGCC TCGGGTCGGG 
     1501 TGGCGTTGGT AAAGGTGCTG GACAAGTGGC CGCTCCGGAG CGGGGGGCTG CAGCGCGAGC 
     1561 AGGTGATCAG CGTGGGCTCC TGTGTGCCGC TCGAAAGGAA CCAGCGCTAC ATCTTTTTCC 
     1621 TGGAGCCCAC GGAACAGCCC TTAGTCTTTA AGACGGCCTT TGCCCCCCTC GATACCAACG 
     1681 GCAAAAATCT CAAGAAAGAG GTGGGCAAGA TCCTGTGCAC TGACTGCGCC ACCCGGCCCA 
     1741 AGTTGAAGAA GATGAAGAGC CAGACGGGAC AGGTGGGTGA GAAGCAATCG CTGAAGTGTG 
     1801 AGGCAGCAGC CGGTAATCCC CAGCCTTCCT ACCGTTGGTT CAAGGATGGC AAGGAGCTCA 
     1861 ACCGCAGCCG AGACATTCGC ATCAAATATG GCAACGGCAG AAAGAACTCA CGACTACAGT 
     1921 TCAACAAGGT GAAGGTGGAG GACGCTGGGG AGTATGTCTG CGAGGCCGAG AACATCCTGG 
     1981 GGAAGGACAC CGTCCGGGGC CGGCTTTACG TCAACAGCGA AGCCGAGGAG CTGTACCAGA 
     2041 AGAGGGTCCT GACCATCACG GGCATCTGCG TGGCTCTGCT GGTCGTGGGC ATCGTCTGTG 
     2101 TGGTGGCCTA CTGCAAGACC AAAAAACAGC GGAAGCAGAT GCACAACCAC CTCCGGCAGA 
     2161 ACATGTGCCC GGCCCATCAG AACCGGAGCT TGGCCAATGG GCCCAGCCAC CCCCGGCTGG 
     2221 ACCCAGAGGA GATCCAGATG GCAGATTATA TTTCCAAGAA CGTGCCAGCC ACAGACCATG 
     2281 TCATCAGGAG AGAAACTGAG ACCACCTTCT CTGGGAGCCA CTCCTGTTCT CCTTCTCACC 
     2341 ACTGCTCCAC AGCCACACCC ACCTCCAGCC ACAGACACGA GAGCCACACG TGGAGCCTGG 
     2401 AACGTTCTGA GAGCCTGACT TCTGACTCCC AGTCGGGGAT CATGCTATCA TCAGTGGGTA 
     2461 CCAGCAAATG CAACAGCCCA GCATGTGTGG AGGCCCGGGC AAGGCGGGCA GCAGCCTACA 
     2521 ACCTGGAGGA GCGGCGCAGG GCCACCGCGC CACCCTATCA CGATTCCGTG GACTCCCTTC 
     2581 GCGACTCCCC ACACAGCGAG AGGTACGTGT CGGCCCTGAC CACGCCCGCG CGCCTCTCGC 
     2641 CCGTGGACTT CCACTACTCG CTGGCCACGC AGGTGCCAAC TTTCGAGATC ACGTCCCCCA 
     2701 ACTCGGCGCA CGCCGTGTCG CTGCCGCCGG CGGCGCCCAT CAGTTACCGC CTGGCCGAGC 
     2761 AGCAGCCGTT ACTGCGGCAC CCGGCGCCCC CCGGCCCGGG ACCCGGACCC GGGCCCGGGC 
     2821 CCGGGCCCGG CGCAGACATG CAGCGCAGCT ATGACAGCTA CTATTACCCC GCGGCGGGGC 
     2881 CCGGACCGCG GCGCGGGACC TGCGCGCTCG GCGGCAGCCT GGGCAGCCTG CCTGCCAGCC 
     2941 CCTTCCGCAT CCCCGAGGAC GACGAGTACG AGACCACGCA GGAGTGCGCG CCCCCGCCGC 
     3001 CGCCGCGGCC GCGCGCGCGC GGTGCGTCCC GCAGGACGTC GGCGGGGCCC CGGCGCTGGC 
     3061 GCCGCTCGCG CCTCAACGGG CTGGCGGCGC AGCGCGCACG GGCGGCGAGG GACTCGCTGT 
     3121 CGCTGAGCAG CGGCTCGGGC GGCGGCTCAG CCTCGGCGTC GGACGACGAC GCGGACGACG 
     3181 CGGACGGGGC GCTGGCGGCC GAGAGCACAC CTTTCCTGGG CCTGCGTGGG GCGCACGACG 
     3241 CGCTGCGCTC GGACTCGCCG CCACTGTGCC CGGCGGCCGA CAGCAGGACT TACTACTCAC 
     3301 TGGACAGCCA CAGCACGCGG GCCAGCAGCA GACACAGCCG CGGGCCGCCC CCGCGGGCCA 
     3361 AGCAGGACTC GGCGCCACTC ACGCGTACGC GGCCGCTCGA GATGGAGAGC GACGAGAGCG 
     3421 GCCTGCCCGC CATGGAGATC GAGTGCCGCA TCACCGGCAC CCTGAACGGC GTGGAGTTCG 
     3481 AGCTGGTGGG CGGCGGAGAG GGCACCCCCG AGCAGGGCCG CATGACCAAC AAGATGAAGA 
     3541 GCACCAAAGG CGCCCTGACC TTCAGCCCCT ACCTGCTGAG CCACGTGATG GGCTACGGCT 
     3601 TCTACCACTT CGGCACCTAC CCCAGCGGCT ACGAGAACCC CTTCCTGCAC GCCATCAACA 
     3661 ACGGCGGCTA CACCAACACC CGCATCGAGA AGTACGAGGA CGGCGGCGTG CTGCACGTGA 
     3721 GCTTCAGCTA CCGCTACGAG GCCGGCCGCG TGATCGGCGA CTTCAAGGTG ATGGGCACCG 
     3781 GCTTCCCCGA GGACAGCGTG ATCTTCACCG ACAAGATCAT CCGCAGCAAC GCCACCGTGG 
     3841 AGCACCTGCA CCCCATGGGC GATAACGATC TGGATGGCAG CTTCACCCGC ACCTTCAGCC 
     3901 TGCGCGACGG CGGCTACTAC AGCTCCGTGG TGGACAGCCA CATGCACTTC AAGAGCGCCA 
     3961 TCCACCCCAG CATCCTGCAG AACGGGGGCC CCATGTTCGC CTTCCGCCGC GTGGAGGAGG 
     4021 ATCACAGCAA CACCGAGCTG GGCATCGTGG AGTACCAGCA CGCCTTCAAG ACCCCGGATG 
     4081 CAGATGCCGG TGAAGAAAGA GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4141 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4201 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4261 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4321 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4381 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4441 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4501 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4561 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4621 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     4681 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     4741 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCTTGGCT GTGGAATGTG 
     4801 TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 
     4861 CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 
     4921 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC 
     4981 CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT 
     5041 ATTTATGCAG AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC 
     5101 TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA 
     5161 TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA 
     5221 GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC 
     5281 GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC 
     5341 AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG 
     5401 CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG 
     5461 GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT 
     5521 GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT 
     5581 ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA 
     5641 GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA 
     5701 CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC 
     5761 GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT 
     5821 GGCCGGCTGG GTGTGGCCGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT 
     5881 GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC 
     5941 GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG 
     6001 GGTTCGAAAT GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG 
     6061 CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC 
     6121 TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT 
     6181 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC 
     6241 TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT 
     6301 CGACCTCTAG CTAGAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT 
     6361 ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG 
     6421 CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG 
     6481 GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC 
     6541 GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC 
     6601 GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA 
     6661 ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG 
     6721 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT 
     6781 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA 
     6841 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC 
     6901 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT 
     6961 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG 
     7021 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG 
     7081 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT 
     7141 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC 
     7201 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG 
     7261 CTGGTAGCGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG 
     7321 AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG 
     7381 GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT 
     7441 GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT 
     7501 TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC 
     7561 TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA 
     7621 TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG 
     7681 GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT 
     7741 GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA 
     7801 TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT 
     7861 CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT 
     7921 TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG 
     7981 CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG 
     8041 AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG 
     8101 CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA 
     8161 AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT 
     8221 AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT 
     8281 GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT 
     8341 GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA 
     8401 TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT 
     8461 TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG 
     8521 TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT 
     8581 TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC 
     8641 TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG 
     8701 GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG 
     8761 AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT 
     8821 CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG 
     8881 AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//