LOCUS       RG230484                8861 bp    DNA     circular  SYN 
DEFINITION  SEC23B (tGFP-tagged) - Human Sec23 homolog B (S. cerevisiae) (SEC23B), transcript variant 4
ACCESSION   NM_001172745
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3342..4046 
                     /label="GFP"  
     misc_feature    4093..4678 
                     /label="PolyA signal"  
     misc_feature    6770..7415 
                     /label="ColE1"  
     misc_feature    5132..5926 
                     /label="Neo"  
     misc_feature    4763..5065 
                     /label="SV40 ori"  
     misc_feature    7430..8290 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3321..3327 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3321 
                     /label="SEC23B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGACATAC CTGGAGTTCA TCCAGCAGAA TGAAGAACGG GATGGTGTGC 
     1081 GTTTTAGTTG GAACGTGTGG CCTTCCAGCC GGCTGGAGGC TACAAGAATG GTTGTACCCC 
     1141 TGGCTTGTCT CCTTACTCCT TTGAAAGAAC GTCCAGACCT ACCTCCTGTA CAATATGAAC 
     1201 CTGTGCTTTG CAGCAGGCCA ACTTGTAAAG CTGTTCTCAA CCCACTTTGT CAGGTTGATT 
     1261 ATCGAGCAAA ACTTTGGGCC TGTAATTTCT GTTTTCAAAG AAATCAGTTT CCTCCAGCTT 
     1321 ATGGAGGCAT ATCTGAGGTG AATCAACCTG CCGAATTGAT GCCCCAGTTT TCTACAATTG 
     1381 AGTACGTGAT ACAGCGAGGT GCTCAGTCCC CTCTGATCTT TCTCTATGTG GTTGACACAT 
     1441 GCCTGGAGGA AGATGACCTT CAAGCACTCA AAGAGTCCCT GCAGATGTCC CTGAGTCTTC 
     1501 TTCCTCCAGA TGCTCTGGTG GGTCTGATCA CATTTGGAAG GATGGTGCAG GTTCATGAGC 
     1561 TAAGCTGTGA AGGAATCTCC AAAAGTTATG TCTTCCGAGG GACCAAGGAT TTAACTGCAA 
     1621 AGCAAATACA GGATATGTTG GGCCTGACCA AGCCAGCCAT GCCCATGCAG CAAGCACGAC 
     1681 CTGCACAACC ACAGGAGCAC CCTTTTGCTT CAAGCAGATT TCTGCAGCCT GTTCACAAGA 
     1741 TTGATATGAA CCTCACTGAT CTTCTTGGGG AGCTACAGAG GGACCCATGG CCAGTAACTC 
     1801 AGGGGAAGAG ACCTTTGCGA TCCACTGGTG TGGCTTTGTC CATTGCTGTT GGCTTGCTGG 
     1861 AGGGCACTTT TCCAAACACA GGAGCCAGGA TCATGCTGTT TACTGGAGGT CCCCCTACCC 
     1921 AAGGGCCTGG CATGGTGGTT GGAGATGAAT TAAAGATTCC TATTCGTTCT TGGCATGATA 
     1981 TTGAGAAAGA TAATGCACGA TTCATGAAAA AGGCAACCAA GCACTATGAG ATGCTTGCTA 
     2041 ATCGAACAGC TGCAAATGGT CACTGCATTG ATATTTATGC TTGTGCCCTT GATCAAACTG 
     2101 GACTTTTGGA GATGAAGTGT TGTGCAAATC TTACTGGAGG CTACATGGTA ATGGGAGATT 
     2161 CTTTCAACAC TTCTCTCTTC AAGCAGACAT TCCAAAGAAT CTTTACTAAA GATTTTAATG 
     2221 GAGATTTCCG AATGGCATTT GGTGCTACTT TGGACGTAAA GACCTCTCGG GAACTGAAGA 
     2281 TTGCAGGAGC CATTGGTCCA TGCGTATCTC TGAATGTGAA AGGACCGTGT GTGTCAGAAA 
     2341 ATGAGCTTGG TGTTGGTGGC ACGAGTCAGT GGAAAATCTG TGGCCTAGAT CCTACATCTA 
     2401 CACTTGGCAT CTATTTTGAA GTTGTCAATC AGCACAACAC CCCGATCCCC CAAGGAGGCA 
     2461 GAGGAGCCAT CCAGTTTGTC ACGCATTATC AGCACTCCAG CACCCAGAGA CGCATCCGCG 
     2521 TGACCACCAT CGCCCGAAAT TGGGCAGATG TACAGAGTCA GCTCAGGCAC ATAGAAGCAG 
     2581 CATTTGACCA GGAGGCTGCG GCAGTGTTGA TGGCACGGCT TGGGGTGTTC CGAGCGGAGT 
     2641 CAGAGGAGGG GCCCGATGTG CTCCGGTGGC TGGACCGACA ACTCATCCGA CTGTGTCAAA 
     2701 AGTTTGGACA GTATAACAAA GAAGACCCCA CTTCTTTTAG GTTATCAGAT TCCTTTTCTC 
     2761 TATATCCTCA GTTTATGTTC CATCTGAGAA GATCTCCATT TCTTCAAGTG TTTAACAACA 
     2821 GTCCTGATGA GTCGTCATAT TACAGACATC ATTTTGCCCG GCAGGACCTG ACCCAGTCCC 
     2881 TCATCATGAT CCAGCCCATT CTCTACTCTT ACTCCTTTCA TGGGCCACCA GAGCCAGTAC 
     2941 TCTTGGATAG CAGCAGCATT CTAGCTGACA GAATTTTGCT GATGGATACT TTCTTTCAAA 
     3001 TTGTCATTTA TCTTGGTGAG ACCATAGCCC AGTGGCGTAA AGCTGGCTAC CAGGACATGC 
     3061 CCGAGTATGA AAACTTCAAG CACCTTCTGC AGGCACCACT GGATGATGCT CAAGAAATTC 
     3121 TGCAAGCACG CTTCCCGATG CCACGTTACA TCAACACGGA GCATGGAGGC AGTCAGGCTC 
     3181 GATTCCTTTT GTCCAAAGTG AACCCATCTC AGACACACAA TAACCTGTAT GCTTGGGGAC 
     3241 AGGAAACTGG AGCACCCATC CTAACTGATG ATGTTAGCCT GCAGGTGTTC ATGGACCATT 
     3301 TGAAGAAGCT GGCTGTCTCC AGTGCCTGTA CGCGTACGCG GCCGCTCGAG ATGGAGAGCG 
     3361 ACGAGAGCGG CCTGCCCGCC ATGGAGATCG AGTGCCGCAT CACCGGCACC CTGAACGGCG 
     3421 TGGAGTTCGA GCTGGTGGGC GGCGGAGAGG GCACCCCCGA GCAGGGCCGC ATGACCAACA 
     3481 AGATGAAGAG CACCAAAGGC GCCCTGACCT TCAGCCCCTA CCTGCTGAGC CACGTGATGG 
     3541 GCTACGGCTT CTACCACTTC GGCACCTACC CCAGCGGCTA CGAGAACCCC TTCCTGCACG 
     3601 CCATCAACAA CGGCGGCTAC ACCAACACCC GCATCGAGAA GTACGAGGAC GGCGGCGTGC 
     3661 TGCACGTGAG CTTCAGCTAC CGCTACGAGG CCGGCCGCGT GATCGGCGAC TTCAAGGTGA 
     3721 TGGGCACCGG CTTCCCCGAG GACAGCGTGA TCTTCACCGA CAAGATCATC CGCAGCAACG 
     3781 CCACCGTGGA GCACCTGCAC CCCATGGGCG ATAACGATCT GGATGGCAGC TTCACCCGCA 
     3841 CCTTCAGCCT GCGCGACGGC GGCTACTACA GCTCCGTGGT GGACAGCCAC ATGCACTTCA 
     3901 AGAGCGCCAT CCACCCCAGC ATCCTGCAGA ACGGGGGCCC CATGTTCGCC TTCCGCCGCG 
     3961 TGGAGGAGGA TCACAGCAAC ACCGAGCTGG GCATCGTGGA GTACCAGCAC GCCTTCAAGA 
     4021 CCCCGGATGC AGATGCCGGT GAAGAAAGAG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4081 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4141 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4201 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4261 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4321 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4381 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     4441 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     4501 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     4561 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     4621 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     4681 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCTTGGCTG 
     4741 TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG 
     4801 CAAAGCATGC ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA 
     4861 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT 
     4921 CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA 
     4981 ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCGGCCT CTGAGCTATT CCAGAAGTAG 
     5041 TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC 
     5101 ATTTTCGGAT CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA 
     5161 TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA 
     5221 CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT 
     5281 CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG 
     5341 CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA 
     5401 GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC 
     5461 CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT 
     5521 GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT 
     5581 CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG 
     5641 CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG 
     5701 ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC 
     5761 ATCGACTGTG GCCGGCTGGG TGTGGCCGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT 
     5821 GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT TCCTCGTGCT TTACGGTATC 
     5881 GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAGCG 
     5941 GGACTCTGGG GTTCGAAATG ACCGACCAAG CGACGCCCAA CCTGCCATCA CGAGATTTCG 
     6001 ATTCCACCGC CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT 
     6061 GGATGATCCT CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCC AACTTGTTTA 
     6121 TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT 
     6181 TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT 
     6241 GTATACCGTC GACCTCTAGC TAGAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT 
     6301 GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG 
     6361 CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT 
     6421 TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG 
     6481 GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG 
     6541 TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT 
     6601 CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA 
     6661 AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA 
     6721 ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC 
     6781 CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT 
     6841 CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA 
     6901 GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG 
     6961 ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT 
     7021 CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA 
     7081 CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT 
     7141 GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC 
     7201 AAACCACCGC TGGTAGCGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG 
     7261 GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT 
     7321 CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA 
     7381 ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT 
     7441 ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG 
     7501 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA 
     7561 GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC 
     7621 AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT 
     7681 CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG 
     7741 TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA 
     7801 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG 
     7861 TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA 
     7921 TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG 
     7981 TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT 
     8041 CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA 
     8101 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA 
     8161 GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG 
     8221 TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC 
     8281 GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT 
     8341 ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC 
     8401 CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG 
     8461 CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC 
     8521 CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA 
     8581 ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC 
     8641 TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT 
     8701 TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA 
     8761 ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT 
     8821 TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//