LOCUS       RG230436                8636 bp    DNA     circular  SYN 
DEFINITION  ZNF185 (tGFP-tagged) - Human zinc finger protein 185 (LIM domain) (ZNF185), transcript variant 2
ACCESSION   NM_001178107
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3117..3821 
                     /label="GFP"  
     misc_feature    3868..4453 
                     /label="PolyA signal"  
     misc_feature    6545..7190 
                     /label="ColE1"  
     misc_feature    4907..5701 
                     /label="Neo"  
     misc_feature    4538..4840 
                     /label="SV40 ori"  
     misc_feature    7205..8065 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3096..3102 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3096 
                     /label="ZNF185"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGTATCTCA GCTCTTGGAG GCCGCACCAA AGGGAAGCCT CTGCCACCAG 
     1081 GCGAGGAGGA GCGCAATAAC GTTCTCAAGC AGATGAAAGT GCGAACCACG CTGAAGGGGG 
     1141 ACAAGAGCTG GATTACCAAG CAGGATGAAT CGGAGGGTCG CACCATAGAG CTGCCCTCAG 
     1201 GCCGGAGTCG CGCCACATCC TTTTCATCAG CTGGGGAGGT TCCGAAGCCT AGGCCTCCGA 
     1261 GCACAAGGGC TCCCACTGGC TACATCATCC GGGGAGTGTT CACCAAGCCC ATAGACAGCT 
     1321 CTTCCCAGCC CCAGCAGCAG TTCCCCAAGG CCAACGGGAC TCCAAAAAGT GCTGCCAGTC 
     1381 TGGTGAGAAC AGCTAACGCT GGTCCTCCCC GCCCCTCCTC CTCTGGCTAC AAGATGACCA 
     1441 CTGAGGATTA CAAGAAGCTG GCACCCTACA ATATCAGGCG CAGCTCTACA TCAGGGGACA 
     1501 CCGAGGAGGA GGAGGAGGAG GAGGTGGTGC CATTCTCCTC AGATGAACAG AAACGGAGGT 
     1561 CAGAGGCTGC AAGCGGTGTT CTGAGGAGGA CAGCTCCCCG GGAGCACTCC TACGTCCTGT 
     1621 CAGCGGCCAA GAAGAGCACT GGTCCTACCC AGGAGACACA GGCACCGTTT ATCGCGAAGA 
     1681 GGGTGGAGGT GGTGGAAGAG GACGGGCCTT CTGAGAAGAG CCAGGACCCA CCTGCTCTGG 
     1741 CAAGATCCAC TCCTGGCTCA AACAGTGCGG ATGGAGGCAG GACCAAAGCG TCTCGGGCAA 
     1801 TTTGGATCGA GTGCCTGCCA AGTATGCCTA GCCCCGCTGG GAGCCAGGAG CTCAGGTCTT 
     1861 CCCCAGGCAA CAAAGACAAG GAGGCCCCCT GCTCCAGAGA GCTCCAGAGG GACTTGGCTG 
     1921 GTGAGGAGGC TTTCAGGGCC CCCAACACAG ATGCTGCAAG GTCAAGTGCA CAGTTGAGTG 
     1981 ATGGCAATGT GGGATCCGGA GCCACGGGCT CCCGGCCTGA AGGCTTGGCT GCAGTAGACA 
     2041 TCGGCTCCGA GAGAGGAAGG CTGTGTGCAG CTGCTAGCTT TGCCTCTTTC CTGGAAGACC 
     2101 AGGATGGGCA CAGTGCCAAC TCTCAGTCCT GCAAGCCTAG ACCAGCAGCC ATCAGCTCCA 
     2161 GTGCCACTTC AGTCTCTGCT GTCCCTGCTG ATAGGAAGAG CAACAGCACA GCAGCCCAGG 
     2221 AGGATGCAAA GGCAGACCCA AAGGGGGCCT TGGCTGATTA TGAGGGGAAG GATGTGGCCA 
     2281 CCAGGGTCGG AGAGGCCTGG CAGGAGAGGC CTGGAGCTCC AAGAGGTGGC CAAGGAGACC 
     2341 CAGCTGTACC CGCTCAGCAA CCTGCAGATC CCAGCACCCC AGAGCGGCAG AGCAGCCCCA 
     2401 GCGGATCTGA GCAACTTGTC AGACGAGAGA GTTGTGGCAG CAGCGTGTTG ACTGATTTTG 
     2461 AGGGGAAGGA TGTGGCCACC AAGGTCGGAG AGGCCTGGCA GGACAGGCCT GGAGCCCCAA 
     2521 GAGGTGGCCA AGGAGACCCA GCTGTACCCA CTCAGCAACC TGCAGATCCC AGTACCCCAG 
     2581 AACAGCAGAA CAGCCCCAGC GGATCTGAGC AATTCGTCAG ACGAGAGAGC TGCACCAGCA 
     2641 GGGTGAGGAG CCCCTCGAGC TGCATGGTCA CTGTTACTGT CACTGCCACA TCTGAGCAGC 
     2701 CTCACATTTA TATTCCAGCC CCCGCAAGTG AATTGGACTC CAGCTCTACC ACCAAAGGGA 
     2761 TTCTCTTCGT GAAGGAGTAC GTGAATGCTA GTGAAGTGTC TTCTGGGAAG CCAGTATCTG 
     2821 CACGCTATAG CAACGTCAGC AGCATTGAGG ACTCATTCGC CATGGAGAAG AAGCCTCCAT 
     2881 GTGGCAGCAC TCCATACTCT GAGAGGACAA CTGGAGGGAT CTGTACTTAC TGCAACCGTG 
     2941 AGATCCGAGA CTGTCCAAAG ATTACCCTAG AACATCTTGG TATCTGCTGC CATGAATATT 
     3001 GCTTTAAGTG TGGGATTTGC AGTAAACCGA TGGGCGATCT CCTGGATCAG ATCTTCATTC 
     3061 ACCGTGACAC CATTCACTGT GGGAAATGCT ATGAGAAGCT CTTCACGCGT ACGCGGCCGC 
     3121 TCGAGATGGA GAGCGACGAG AGCGGCCTGC CCGCCATGGA GATCGAGTGC CGCATCACCG 
     3181 GCACCCTGAA CGGCGTGGAG TTCGAGCTGG TGGGCGGCGG AGAGGGCACC CCCGAGCAGG 
     3241 GCCGCATGAC CAACAAGATG AAGAGCACCA AAGGCGCCCT GACCTTCAGC CCCTACCTGC 
     3301 TGAGCCACGT GATGGGCTAC GGCTTCTACC ACTTCGGCAC CTACCCCAGC GGCTACGAGA 
     3361 ACCCCTTCCT GCACGCCATC AACAACGGCG GCTACACCAA CACCCGCATC GAGAAGTACG 
     3421 AGGACGGCGG CGTGCTGCAC GTGAGCTTCA GCTACCGCTA CGAGGCCGGC CGCGTGATCG 
     3481 GCGACTTCAA GGTGATGGGC ACCGGCTTCC CCGAGGACAG CGTGATCTTC ACCGACAAGA 
     3541 TCATCCGCAG CAACGCCACC GTGGAGCACC TGCACCCCAT GGGCGATAAC GATCTGGATG 
     3601 GCAGCTTCAC CCGCACCTTC AGCCTGCGCG ACGGCGGCTA CTACAGCTCC GTGGTGGACA 
     3661 GCCACATGCA CTTCAAGAGC GCCATCCACC CCAGCATCCT GCAGAACGGG GGCCCCATGT 
     3721 TCGCCTTCCG CCGCGTGGAG GAGGATCACA GCAACACCGA GCTGGGCATC GTGGAGTACC 
     3781 AGCACGCCTT CAAGACCCCG GATGCAGATG CCGGTGAAGA AAGAGTTTAA ACGGCCGGCC 
     3841 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     3901 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     3961 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4021 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4081 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4141 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4201 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4261 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     4321 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     4381 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     4441 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     4501 CGGCCAGCTT GGCTGTGGAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA 
     4561 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCAGGTG TGGAAAGTCC 
     4621 CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCATA 
     4681 GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG 
     4741 CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC GGCCTCTGAG 
     4801 CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG 
     4861 GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG 
     4921 ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC 
     4981 TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG 
     5041 CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG 
     5101 GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC 
     5161 GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT 
     5221 CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG 
     5281 CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC 
     5341 GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG 
     5401 CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC 
     5461 GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC 
     5521 CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CCGACCGCTA TCAGGACATA 
     5581 GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC 
     5641 GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC 
     5701 GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC 
     5761 CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT 
     5821 TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC 
     5881 ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT 
     5941 TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG 
     6001 TATCTTATCA TGTCTGTATA CCGTCGACCT CTAGCTAGAG CTTGGCGTAA TCATGGTCAT 
     6061 AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA 
     6121 GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC 
     6181 GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC 
     6241 AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT 
     6301 CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC 
     6361 GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA 
     6421 AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG 
     6481 ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA 
     6541 GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC 
     6601 TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC 
     6661 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC 
     6721 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG 
     6781 TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT 
     6841 ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA 
     6901 CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT 
     6961 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTTTTTT TGTTTGCAAG CAGCAGATTA 
     7021 CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC 
     7081 AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA 
     7141 CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA 
     7201 CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT 
     7261 TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT 
     7321 TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT 
     7381 TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT 
     7441 CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA 
     7501 ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG 
     7561 GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT 
     7621 TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG 
     7681 CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG 
     7741 TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC 
     7801 GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA 
     7861 CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC 
     7921 CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT 
     7981 TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG 
     8041 GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA 
     8101 GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA 
     8161 AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG 
     8221 GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG 
     8281 CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC 
     8341 CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC 
     8401 TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA 
     8461 CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA 
     8521 CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA 
     8581 TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//