LOCUS       RG230178                8060 bp    DNA     circular  SYN 
DEFINITION  MEF2A (tGFP-tagged) - Human myocyte enhancer factor 2A (MEF2A), transcript variant 5
ACCESSION   NM_001171894
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2539..3243 
                     /label="GFP"  
     misc_feature    3290..3875 
                     /label="PolyA signal"  
     misc_feature    5967..6612 
                     /label="ColE1"  
     misc_feature    4329..5123 
                     /label="Neo"  
     misc_feature    3960..4262 
                     /label="SV40 ori"  
     misc_feature    6627..7487 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2511..2518 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2511 
                     /label="MEF2A"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGCGGAAG AAAATACAAA TCACACGCAT AATGGATGAA AGGAACCGAC 
     1081 AGGTCACTTT TACAAAGAGA AAGTTTGGAT TAATGAAGAA AGCCTATGAA CTTAGTGTGC 
     1141 TCTGTGACTG TGAAATAGCA CTCATCATTT TCAACAGCTC TAACAAACTG TTTCAATATG 
     1201 CTAGCACTGA TATGGACAAA GTTCTTCTCA AGTATACAGA ATATAATGAA CCTCATGAAA 
     1261 GCAGAACCAA CTCGGATATT GTTGAGACTT TAAGAAAGAA AGGCCTTAAT GGTTGTGAGA 
     1321 GCCCTGATGC TGACGATTAC TTTGAGCACA GTCCACTCTC GGAGGACAGA TTCAGCAAAC 
     1381 TAAATGAAGA TAGTGATTTT ATTTTCAAAC GAGGCCCTCC TGGTCTGCCA CCTCAGAACT 
     1441 TTTCAATGTC TGTCACAGTT CCAGTGACCA GCCCCAATGC TTTGTCCTAC ACTAACCCAG 
     1501 GGAGTTCACT GGTGTCCCCA TCTTTGGCAG CCAGCTCAAC GTTAACAGAT TCAAGCATGC 
     1561 TCTCTCCACC TCAAACCACA TTACATAGAA ATGTGTCTCC TGGAGCTCCT CAGAGACCAC 
     1621 CAAGTACTGG CAATGCAGGT GGGATGTTGA GCACTACAGA CCTCACAGTG CCAAATGGAG 
     1681 CTGGAAGCAG TCCAGTGGGG AATGGATTTG TAAACTCAAG AGCTTCTCCA AATTTGATTG 
     1741 GAGCTACTGG TGCAAATAGC TTAGGCAAAG TCATGCCTAC AAAGTCTCCC CCTCCACCAG 
     1801 GTGGTGGTAA TCTTGGAATG AACAGTAGGA AACCAGATCT TCGAGTTGTC ATCCCCCCTT 
     1861 CAAGCAAGGG CATGATGCCT CCACTAAATA CCCAAAGGAT CAGTAGTTCT CAAGCCACTC 
     1921 AACCTCTTGC TACCCCAGTC GTGTCTGTGA CAACCCCAAG CTTGCCTCCG CAAGGACTTG 
     1981 TGTACTCAGC AATGCCGACT GCCTACAACA CTGATTATTC ACTGACCAGC GCTGACCTGT 
     2041 CAGCCCTTCA AGGCTTCAAC TCGCCAGGAA TGCTGTCGCT GGGACAGGTG TCGGCCTGGC 
     2101 AGCAGCACCA CCTAGGACAA GCAGCCCTCA GCTCTCTTGT TGCTGGAGGG CAGTTATCTC 
     2161 AGGGTTCCAA TTTATCCATT AATACCAACC AAAACATCAG CATCAAGTCC GAACCGATTT 
     2221 CACCTCCTCG GGATCGTATG ACCCCATCGG GCTTCCAGCA GCAGCAGCAG CAGCAGCAGC 
     2281 AGCAGCAGCC GCCGCCACCA CCGCAGCCCC AGCCACAACC CCCGCAGCCC CAGCCCCGAC 
     2341 AGGAAATGGG GCGCTCCCCT GTGGACAGTC TGAGCAGCTC TAGTAGCTCC TATGATGGCA 
     2401 GTGATCGGGA GGATCCACGG GGCGACTTCC ATTCTCCAAT TGTGCTTGGC CGACCCCCAA 
     2461 ACACTGAGGA CAGAGAAAGC CCTTCTGTAA AGCGAATGAG GATGGACGCG TGGGTGACCA 
     2521 GCGGACCGAC GCGTACGCGG CCGCTCGAGA TGGAGAGCGA CGAGAGCGGC CTGCCCGCCA 
     2581 TGGAGATCGA GTGCCGCATC ACCGGCACCC TGAACGGCGT GGAGTTCGAG CTGGTGGGCG 
     2641 GCGGAGAGGG CACCCCCGAG CAGGGCCGCA TGACCAACAA GATGAAGAGC ACCAAAGGCG 
     2701 CCCTGACCTT CAGCCCCTAC CTGCTGAGCC ACGTGATGGG CTACGGCTTC TACCACTTCG 
     2761 GCACCTACCC CAGCGGCTAC GAGAACCCCT TCCTGCACGC CATCAACAAC GGCGGCTACA 
     2821 CCAACACCCG CATCGAGAAG TACGAGGACG GCGGCGTGCT GCACGTGAGC TTCAGCTACC 
     2881 GCTACGAGGC CGGCCGCGTG ATCGGCGACT TCAAGGTGAT GGGCACCGGC TTCCCCGAGG 
     2941 ACAGCGTGAT CTTCACCGAC AAGATCATCC GCAGCAACGC CACCGTGGAG CACCTGCACC 
     3001 CCATGGGCGA TAACGATCTG GATGGCAGCT TCACCCGCAC CTTCAGCCTG CGCGACGGCG 
     3061 GCTACTACAG CTCCGTGGTG GACAGCCACA TGCACTTCAA GAGCGCCATC CACCCCAGCA 
     3121 TCCTGCAGAA CGGGGGCCCC ATGTTCGCCT TCCGCCGCGT GGAGGAGGAT CACAGCAACA 
     3181 CCGAGCTGGG CATCGTGGAG TACCAGCACG CCTTCAAGAC CCCGGATGCA GATGCCGGTG 
     3241 AAGAAAGAGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     3301 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     3361 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     3421 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     3481 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     3541 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     3601 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     3661 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     3721 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     3781 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     3841 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     3901 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCTTGGCTGT GGAATGTGTG TCAGTTAGGG 
     3961 TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG 
     4021 TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 
     4081 CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT 
     4141 CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG 
     4201 GCCGAGGCCG CCTCGGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC 
     4261 CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAAGAG 
     4321 ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC 
     4381 GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT 
     4441 GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG 
     4501 TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG 
     4561 GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA 
     4621 TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA 
     4681 TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC 
     4741 GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC 
     4801 GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG 
     4861 CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG 
     4921 CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT 
     4981 GTGGCCGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC 
     5041 GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC 
     5101 ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG GACTCTGGGG TTCGAAATGA 
     5161 CCGACCAAGC GACGCCCAAC CTGCCATCAC GAGATTTCGA TTCCACCGCC GCCTTCTATG 
     5221 AAAGGTTGGG CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG 
     5281 ATCTCATGCT GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA 
     5341 AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT 
     5401 GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG ACCTCTAGCT 
     5461 AGAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA 
     5521 TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA 
     5581 GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT 
     5641 GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT 
     5701 CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT 
     5761 CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA 
     5821 ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT 
     5881 TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT 
     5941 GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC 
     6001 GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA 
     6061 GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT 
     6121 CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA 
     6181 ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG 
     6241 GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC 
     6301 CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA 
     6361 CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTT 
     6421 TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA 
     6481 TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA 
     6541 TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT 
     6601 CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG 
     6661 CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT 
     6721 AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG 
     6781 ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC 
     6841 GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG 
     6901 CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA 
     6961 TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA 
     7021 GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA 
     7081 TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA 
     7141 ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA 
     7201 AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG 
     7261 ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG 
     7321 GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG 
     7381 CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG 
     7441 GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC 
     7501 TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA 
     7561 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG 
     7621 TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA 
     7681 GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT 
     7741 TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT 
     7801 TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC 
     7861 GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT 
     7921 TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT 
     7981 TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC 
     8041 AAAAATTTAA CGCGAATTTT 
//