LOCUS       RG229402                9545 bp    DNA     circular  SYN 
DEFINITION  TMEM67 (tGFP-tagged) - Human transmembrane protein 67 (TMEM67), transcript variant 1
ACCESSION   NM_153704
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4026..4730 
                     /label="GFP"  
     misc_feature    4777..5362 
                     /label="PolyA signal"  
     misc_feature    7454..8099 
                     /label="ColE1"  
     misc_feature    5816..6610 
                     /label="Neo"  
     misc_feature    5447..5749 
                     /label="SV40 ori"  
     misc_feature    8114..8974 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4005..4011 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4005 
                     /label="TMEM67"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGACGCGC GGTGGGGCTG GGGTGGCAAT GGCGGTTTGG TCCCTCTTAT 
     1081 CCGCCCGGGC CGTGACCGCG TTCCTTCTGT TGTTCCTCCC TCGCTTCTTA CAGGCCCAGA 
     1141 CCTTCTCTTT CCCTTTCCAG CAGCCGGAGA AGTGCGACAA CAACCAGTAC TTTGATATCT 
     1201 CCGCCCTCTC GTGTGTTCCT TGTGGAGCTA ACCAGAGGCA AGATGCCCGA GGAACTTCAT 
     1261 GTGTATGTCT ACCAGGATTT CAGATGATCT CTAATAATGG AGGACCTGCT ATTATTTGTA 
     1321 AAAAGTGCCC AGAAAACATG AAAGGTGTTA CAGAAGATGG CTGGAACTGC ATTTCTTGCC 
     1381 CTAGTGACTT AACTGCCGAA GGAAAATGTC ACTGTCCCAT TGGCCATATT TTAGTGGAAA 
     1441 GAGACATTAA TGGAACATTG TTGTCTCAAG CAACTTGTGA GCTCTGTGAT GGAAATGAAA 
     1501 ACTCTTTTAT GGTAGTAAAT GCTTTAGGAG ACAGGTGCGT CCGATGTGAG CCAACATTTG 
     1561 TTAATACCAG CAGGTCCTGT GCATGTTCAG AACCTAACAT TTTAACAGGG GGATTATGTT 
     1621 TCAGCAGCAC AGGGAATTTT CCTCTACGTA GAATTTCAGC TGCACGTTAT GGAGAAGTTG 
     1681 GCATGTCTTT AACTTCAGAA TGGTTTGCAA AGTATTTGCA ATCATCAGCA GCTGCATGTT 
     1741 GGGTATATGC CAATCTAACA TCTTGTCAAG CTCTTGGAAA TATGTGTGTG ATGAACATGA 
     1801 ATTCTTACGA CTTTGCCACA TTTGATGCAT GTGGACTATT TCAGTTTATC TTTGAAAATA 
     1861 CTGCTGGACT GAGCACTGTT CATTCTATTT CATTTTGGAG ACAGAATCTT CCTTGGCTGT 
     1921 TTTATGGAGA CCAGTTAGGA TTAGCACCTC AAGTGCTCAG TTCTACCTCT CTTCCTACAA 
     1981 ATTTCAGTTT TAAAGGAGAA AACCAGAATA CAAAACTGAA GTTTGTTGCT GCTTCCTATG 
     2041 ATATAAGAGG AAATTTTCTC AAGTGGCAAA CTTTAGAAGG AGGTGTTTTA CAGCTTTGTC 
     2101 CAGACACAGA GACAAGGCTA AATGCTGCTT ATTCATTTGG AACAACCTAC CAACAAAATT 
     2161 GTGAGATTCC TATCTCTAAG ATCTTAATTG ACTTTCCCAC TCCTATATTT TATGATGTGT 
     2221 ACCTTGAATA TACTGATGAA AATCAACATC AATATATTTT GGCTGTGCCT GTGTTAAACC 
     2281 TAAATCTTCA ACATAATAAG ATATTTGTGA ACCAAGACAG CAACTCTGGA AAGTGGCTTC 
     2341 TAACTCGGCG CATTTTCTTA GTGGATGCAG TAAGTGGACG AGAAAATGAC TTAGGAACTC 
     2401 AGCCAAGAGT AATTCGAGTT GCTACTCAAA TATCACTGAG TGTCCACCTT GTACCCAACA 
     2461 CAATAAATGG AAACATCTAC CCTCCCTTAA TCACCATTGC CTACAGTGAC ATTGATATCA 
     2521 AAGATGCCAA CAGCCAGTCT GTGAAGGTTT CTTTCTCAGT CACATATGAA ATGGATCATG 
     2581 GAGAAGCACA TGTCCAGACA GATATTGCTT TGGGTGTATT GGGTGGGCTA GCTGTTTTAG 
     2641 CATCTCTTTT GAAGACAGCA GGATGGAAGA GGCGCATTGG GAGTCCCATG ATTGATTTAC 
     2701 AGACAGTTGT GAAATTCTTG GTGTACTATG CTGGTGATCT GGCCAATGTT TTCTTTATCA 
     2761 TCACAGTGGG AACAGGTCTT TACTGGCTTA TTTTCTTCAA AGCACAGAAG TCTGTGTCTG 
     2821 TTTTGCTGCC AATGCCAATT CAGGAAGAAC GTTTTGTCAC TTATGTTGGA TGTGCCTTTG 
     2881 CTCTGAAGGC ACTACAATTT TTGCATAAGC TCATATCCCA GATTACAATA GATGTATTCT 
     2941 TTATTGATTG GGAGCGACCT AAAGGAAAGG TTCTTAAAGC TGTTGAAGGT GAGGGTGGTG 
     3001 TACGAAGTGC CACTGTTCCT GTAAGCATAT GGAGAACATA TTTTGTAGCA AATGAATGGA 
     3061 ATGAAATTCA GACTGTGAGA AAAATTAATT CACTCTTTCA AGTACTTACT GTCCTCTTCT 
     3121 TTTTGGAGGT TGTGGGATTC AAGAACTTAG CATTAATGGA CTCATCTTCT AGTCTTTCTA 
     3181 GAAACCCACC TAGCTACATA GCTCCTTATA GCTGCATTTT GAGATATGCA GTGTCTGCTG 
     3241 CTCTTTGGCT AGCCATTGGA ATTATACAGG TCGTGTTCTT TGCTGTCTTT TATGAGAGAT 
     3301 TTATAGAAGA TAAAATTCGA CAGTTCGTTG ATTTATGCTC TATGAGTAAT ATATCAGTGT 
     3361 TTCTGTTATC CCACAAATGT TTTGGATATT ACATTCATGG TAGATCAGTA CATGGGCATG 
     3421 CAGATACTAA TATGGAAGAA ATGAATATGA ACCTTAAAAG AGAAGCGGAA AATTTGTGTA 
     3481 GCCAGAGAGG TTTGGTACCC AACACAGATG GTCAGACTTT TGAGATTGCA ATTTCTAACC 
     3541 AGATGAGACA ACATTATGAC AGAATTCATG AGACACTAAT AAGGAAAAAT GGTCCTGCTA 
     3601 GACTACTGAG TTCATCAGCA AGTACTTTTG AGCAGAGTAT AAAAGCATAT CATATGATGA 
     3661 ATAAATTTCT TGGCTCCTTC ATTGACCATG TTCATAAGGA AATGGATTAC TTTATAAAAG 
     3721 ATAAGTTGCT TCTTGAAAGA ATTCTTGGAA TGGAATTCAT GGAACCAATG GAAAAAAGCA 
     3781 TCTTTTACAA TGATGAAGGT TATTCTTTCA GCAGTGTCCT GTATTATGGA AATGAAGCTA 
     3841 CTCTTCTTAT TTTTGATCTG CTGTTCTTCT GTGTTGTGGA TTTGGCTTGC CAAAATTTTA 
     3901 TTTTAGCATC CTTCCTTACA TATCTACAAC AAGAGATTTT TAGATATATC CGTAATACAG 
     3961 TAGGACAAAA GAATTTGGCA TCCAAAACAT TGGTGGATCA AAGATTTTTG ATTACGCGTA 
     4021 CGCGGCCGCT CGAGATGGAG AGCGACGAGA GCGGCCTGCC CGCCATGGAG ATCGAGTGCC 
     4081 GCATCACCGG CACCCTGAAC GGCGTGGAGT TCGAGCTGGT GGGCGGCGGA GAGGGCACCC 
     4141 CCGAGCAGGG CCGCATGACC AACAAGATGA AGAGCACCAA AGGCGCCCTG ACCTTCAGCC 
     4201 CCTACCTGCT GAGCCACGTG ATGGGCTACG GCTTCTACCA CTTCGGCACC TACCCCAGCG 
     4261 GCTACGAGAA CCCCTTCCTG CACGCCATCA ACAACGGCGG CTACACCAAC ACCCGCATCG 
     4321 AGAAGTACGA GGACGGCGGC GTGCTGCACG TGAGCTTCAG CTACCGCTAC GAGGCCGGCC 
     4381 GCGTGATCGG CGACTTCAAG GTGATGGGCA CCGGCTTCCC CGAGGACAGC GTGATCTTCA 
     4441 CCGACAAGAT CATCCGCAGC AACGCCACCG TGGAGCACCT GCACCCCATG GGCGATAACG 
     4501 ATCTGGATGG CAGCTTCACC CGCACCTTCA GCCTGCGCGA CGGCGGCTAC TACAGCTCCG 
     4561 TGGTGGACAG CCACATGCAC TTCAAGAGCG CCATCCACCC CAGCATCCTG CAGAACGGGG 
     4621 GCCCCATGTT CGCCTTCCGC CGCGTGGAGG AGGATCACAG CAACACCGAG CTGGGCATCG 
     4681 TGGAGTACCA GCACGCCTTC AAGACCCCGG ATGCAGATGC CGGTGAAGAA AGAGTTTAAA 
     4741 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4801 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4861 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4921 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4981 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     5041 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5101 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5161 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5221 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5281 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5341 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5401 TTGGGTACGC GGCCAGCTTG GCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA 
     5461 GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT 
     5521 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA 
     5581 GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC 
     5641 CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCG 
     5701 GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA 
     5761 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG 
     5821 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG 
     5881 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG 
     5941 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT 
     6001 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA 
     6061 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG 
     6121 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT 
     6181 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA 
     6241 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG 
     6301 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG 
     6361 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG 
     6421 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC CGACCGCTAT 
     6481 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC 
     6541 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC 
     6601 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC 
     6661 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG 
     6721 GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT 
     6781 TCTTCGCCCA CCCCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA 
     6841 TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC 
     6901 TCATCAATGT ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT 
     6961 CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC 
     7021 GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA 
     7081 TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT 
     7141 GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC 
     7201 TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG 
     7261 CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG 
     7321 GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC 
     7381 GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG 
     7441 GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA 
     7501 CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC 
     7561 ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG 
     7621 TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT 
     7681 CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA 
     7741 GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA 
     7801 CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG 
     7861 TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTTTTTTT GTTTGCAAGC 
     7921 AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT 
     7981 CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA 
     8041 GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT 
     8101 ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA 
     8161 TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC 
     8221 GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG 
     8281 CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG 
     8341 CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT 
     8401 CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT 
     8461 CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT 
     8521 CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA 
     8581 AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA 
     8641 TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT 
     8701 AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC 
     8761 ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA 
     8821 GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT 
     8881 CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG 
     8941 CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT 
     9001 ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT 
     9061 AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC 
     9121 CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC 
     9181 TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG 
     9241 CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT 
     9301 TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC 
     9361 CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT 
     9421 TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA 
     9481 TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA 
     9541 ATTTT
//