LOCUS       RG229039                9317 bp    DNA     circular  SYN 
DEFINITION  AP1B1 (tGFP-tagged) - Human adaptor-related protein complex 1, beta 1 subunit (AP1B1), transcript variant 3
ACCESSION   NM_001166019
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3798..4502 
                     /label="GFP"  
     misc_feature    4549..5134 
                     /label="PolyA signal"  
     misc_feature    7226..7871 
                     /label="ColE1"  
     misc_feature    5588..6382 
                     /label="Neo"  
     misc_feature    5219..5521 
                     /label="SV40 ori"  
     misc_feature    7886..8746 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3777..3783 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3777 
                     /label="AP1B1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACTGACTCA AAATATTTCA CCACGACCAA GAAAGGGGAG ATCTTCGAGC 
     1081 TGAAGGCAGA GCTCAACAGT GACAAGAAGG AGAAGAAGAA GGAGGCAGTG AAGAAAGTGA 
     1141 TTGCATCGAT GACCGTGGGC AAAGATGTCA GTGCCCTCTT CCCCGATGTG GTCAACTGCA 
     1201 TGCAGACGGA CAACCTGGAG CTGAAGAAGC TGGTATACCT CTACTTGATG AATTACGCCA 
     1261 AGAGTCAGCC TGACATGGCC ATTATGGCCG TCAACACCTT TGTGAAGGAC TGTGAGGACC 
     1321 CCAACCCCCT CATCCGAGCC CTGGCAGTGC GGACCATGGG CTGCATCCGC GTTGACAAGA 
     1381 TCACAGAGTA CCTGTGCGAG CCACTCCGGA AGTGCCTGAA GGACGAGGAT CCATATGTGC 
     1441 GCAAGACAGC AGCTGTGTGC GTGGCCAAGC TCCACGACAT CAACGCCCAG CTGGTGGAGG 
     1501 ACCAGGGCTT CCTGGACACC CTTAAAGACC TCATCTCCGA CTCTAACCCC ATGGTGGTGG 
     1561 CCAATGCAGT GGCAGCGCTC TCAGAAATTG CCGAGTCTCA CCCCAGCAGC AACCTGCTCG 
     1621 ATCTGAACCC ACAGTCCATC AACAAGCTGC TGACAGCCCT CAATGAGTGC ACCGAGTGGG 
     1681 GCCAGATCTT CATCCTGGAC TGCCTCGCCA ACTATATGCC CAAGGACGAC CGCGAGGCCC 
     1741 AGAGCATCTG TGAGCGGGTC ACCCCCAGGC TCTCCCATGC CAACTCCGCT GTGGTGCTCT 
     1801 CTGCTGTGAA GGTGCTGATG AAGTTCATGG AGATGTTGTC TAAGGACTTG GACTACTACG 
     1861 GCACACTGCT CAAGAAGCTG GCCCCACCCC TGGTCACACT GCTGTCAGCC GAGCCAGAGC 
     1921 TGCAGTATGT GGCCCTGCGC AACATCAATC TCATCGTGCA GAAAAGGCCT GAGATCCTGA 
     1981 AGCATGAGAT GAAGGTGTTC TTCGTGAAGT ACAACGACCC TATCTACGTG AAGCTGGAGA 
     2041 AGCTGGACAT CATGATCCGC CTGGCCTCTC AGGCCAACAT CGCCCAGGTG TTGGCAGAGC 
     2101 TGAAAGAGTA CGCAACAGAA GTGGATGTGG ACTTTGTACG GAAGGCTGTG CGTGCTATTG 
     2161 GCCGCTGCGC CATCAAGGTG GAGCAATCTG CGGAGCGCTG TGTGAGCACG CTGCTCGACC 
     2221 TCATCCAGAC CAAGGTCAAC TATGTGGTCC AGGAGGCCAT CGTGGTCATC AAGGACATCT 
     2281 TCCGCAAGTA CCCCAACAAG TATGAGAGTG TGATTGCCAC ACTGTGTGAG AATCTGGACT 
     2341 CCCTGGATGA GCCTGAGGCC CGGGCTGCCA TGATCTGGAT TGTGGGCGAG TACGCGGAAC 
     2401 GGATCGACAA CGCAGATGAG CTGCTGGAGA GCTTCCTCGA GGGCTTCCAT GACGAGAGCA 
     2461 CACAGGTCCA GCTGCAGCTG CTGACAGCCA TTGTGAAACT CTTTCTAAAG AAGCCAACAG 
     2521 AGACCCAGGA GCTGGTGCAG CAGGTCCTCA GTTTGGCCAC TCAGGACTCA GATAACCCAG 
     2581 ACCTGCGGGA CCGTGGCTAC ATCTACTGGC GCCTGCTGTC CACGGACCCG GTGGCAGCCA 
     2641 AGGAGGTGGT GTTGGCTGAG AAGCCACTCA TCTCTGAAGA GACGGACCTC ATCGAGCCCA 
     2701 CACTGTTAGA CGAGCTTATC TGCTACATCG GCACGCTGGC TTCCGTCTAC CATAAGCCTC 
     2761 CCAGTGCCTT TGTGGAGGGG GGCCGGGGCG TCGTGCACAA GAGCCTGCCA CCTCGCACGG 
     2821 CCTCGAGTGA GAGCGCAGAG AGCCCTGAGA CAGCCCCTAC TGGAGCACCT CCTGGGGAGC 
     2881 AGCCAGATGT CATCCCCGCC CAGGGCGACC TGCTGGGTGA CCTCCTCAAC CTGGACCTCG 
     2941 GCCCCCCAGT GAGCGGCCCA CCCCTGGCCA CCTCCTCGGT GCAGATGGGA GCTGTGGACC 
     3001 TTCTTGGCGG TGGCCTTGAC AGCCTGATTG GGGGCACCAA CTTCGTGGCA CCTCCAACAG 
     3061 CAGCAGTACC AGCCAATCTT GGAGCACCCA TCGGCAGTGG CCTGAGTGAC CTCTTTGACC 
     3121 TGACCAGTGG CGTGGGCACC CTGTCAGGAT CATATGTGGC CCCCAAAGCA GTGGGCTCCA 
     3181 TCTCCATGGA CCTGCAGCTG ACCAACAAGG CCTTGCAGGT CATGACCGAC TTTGCCATCC 
     3241 AGTTCAACCG CAACAGCTTT GGCCTGGCCC CCGCCGCCCC CCTCCAGGTC CACGCACCAC 
     3301 TCAGCCCCAA CCAGACAGTG GAGATCTCCC TGCCTCTCAG CACGGTGGGC TCGGTCATGA 
     3361 AGATGGAGCC TCTGAACAAC CTCCAGGTGG CCGTGAAGAA CAACATCGAT GTCTTCTACT 
     3421 TCAGCACCTT GTACCCACTG CACATCCTCT TTGTGGAGGA CGGGAAGATG GACCGGCAGA 
     3481 TGTTCCTGGC CACATGGAAG GATATTCCCA ATGAGAATGA GGCCCAGTTC CAGATCAGAG 
     3541 ACTGCCCCCT CAATGCAGAG GCTGCGAGCA GCAAGCTGCA GAGCAGCAAC ATCTTCACTG 
     3601 TCGCCAAGAG GAACGTGGAG GGCCAGGACA TGCTCTACCA GTCCCTGAAG CTGACCAACG 
     3661 GCATCTGGGT GCTGGCGGAG CTGCGGATCC AGCCGGGCAA CCCCAGCTGC ACGCTGTCCC 
     3721 TGAAGTGTCG AGCACCAGAG GTGTCCCAGC ACGTGTACCA GGCCTACGAG ACCATCCTCA 
     3781 AGAACACGCG TACGCGGCCG CTCGAGATGG AGAGCGACGA GAGCGGCCTG CCCGCCATGG 
     3841 AGATCGAGTG CCGCATCACC GGCACCCTGA ACGGCGTGGA GTTCGAGCTG GTGGGCGGCG 
     3901 GAGAGGGCAC CCCCGAGCAG GGCCGCATGA CCAACAAGAT GAAGAGCACC AAAGGCGCCC 
     3961 TGACCTTCAG CCCCTACCTG CTGAGCCACG TGATGGGCTA CGGCTTCTAC CACTTCGGCA 
     4021 CCTACCCCAG CGGCTACGAG AACCCCTTCC TGCACGCCAT CAACAACGGC GGCTACACCA 
     4081 ACACCCGCAT CGAGAAGTAC GAGGACGGCG GCGTGCTGCA CGTGAGCTTC AGCTACCGCT 
     4141 ACGAGGCCGG CCGCGTGATC GGCGACTTCA AGGTGATGGG CACCGGCTTC CCCGAGGACA 
     4201 GCGTGATCTT CACCGACAAG ATCATCCGCA GCAACGCCAC CGTGGAGCAC CTGCACCCCA 
     4261 TGGGCGATAA CGATCTGGAT GGCAGCTTCA CCCGCACCTT CAGCCTGCGC GACGGCGGCT 
     4321 ACTACAGCTC CGTGGTGGAC AGCCACATGC ACTTCAAGAG CGCCATCCAC CCCAGCATCC 
     4381 TGCAGAACGG GGGCCCCATG TTCGCCTTCC GCCGCGTGGA GGAGGATCAC AGCAACACCG 
     4441 AGCTGGGCAT CGTGGAGTAC CAGCACGCCT TCAAGACCCC GGATGCAGAT GCCGGTGAAG 
     4501 AAAGAGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4561 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4621 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4681 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4741 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4801 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4861 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4921 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4981 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5041 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5101 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5161 ATGCCTGTTG AATTGGGTAC GCGGCCAGCT TGGCTGTGGA ATGTGTGTCA GTTAGGGTGT 
     5221 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA 
     5281 GCAACCAGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 
     5341 CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG 
     5401 CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC 
     5461 GAGGCCGCCT CGGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA 
     5521 GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA 
     5581 GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT 
     5641 TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC 
     5701 GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC 
     5761 GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC 
     5821 GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG 
     5881 GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC 
     5941 ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC 
     6001 CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT 
     6061 CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC 
     6121 AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG 
     6181 AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG 
     6241 GCCGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC 
     6301 GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC 
     6361 GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GAAATGACCG 
     6421 ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA 
     6481 GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC 
     6541 TCATGCTGGA GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT 
     6601 AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG 
     6661 GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA 
     6721 GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC 
     6781 CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT 
     6841 AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC 
     6901 AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT 
     6961 CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG 
     7021 CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA 
     7081 TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT 
     7141 TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC 
     7201 GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT 
     7261 CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG 
     7321 TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA 
     7381 AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT 
     7441 ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA 
     7501 ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA 
     7561 ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT 
     7621 TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTTTTT 
     7681 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT 
     7741 TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA 
     7801 GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA 
     7861 TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC 
     7921 CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA 
     7981 TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC 
     8041 CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA 
     8101 GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA 
     8161 GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG 
     8221 TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC 
     8281 GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG 
     8341 TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT 
     8401 CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT 
     8461 CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA 
     8521 ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC 
     8581 GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC 
     8641 CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA 
     8701 GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT 
     8761 TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT 
     8821 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC 
     8881 CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG 
     8941 TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC 
     9001 TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC 
     9061 GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA 
     9121 GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA 
     9181 ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG 
     9241 ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA 
     9301 AATTTAACGC GAATTTT
//