LOCUS       RG229033                9170 bp    DNA     circular  SYN 
DEFINITION  SULF2 (tGFP-tagged) - Human sulfatase 2 (SULF2), transcript variant 3
ACCESSION   NM_001161841
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3651..4355 
                     /label="GFP"  
     misc_feature    4402..4987 
                     /label="PolyA signal"  
     misc_feature    7079..7724 
                     /label="ColE1"  
     misc_feature    5441..6235 
                     /label="Neo"  
     misc_feature    5072..5374 
                     /label="SV40 ori"  
     misc_feature    7739..8599 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3630..3636 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3630 
                     /label="SULF2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCCCCCCG AGCCTCGTGC TGTGCTTGCT GTCCGCAACT GTGTTCTCCC 
     1081 TGCTGGGTGG AAGCTCGGCC TTCCTGTCGC ACCACCGCCT GAAAGGCAGG TTTCAGAGGG 
     1141 ACCGCAGGAA CATCCGCCCC AACATCATCC TGGTGCTGAC GGACGACCAG GATGTGGAGC 
     1201 TGGGTTCCAT GCAGGTGATG AACAAGACCC GGCGCATCAT GGAGCAGGGC GGGGCGCACT 
     1261 TCATCAACGC CTTCGTGACC ACACCCATGT GCTGCCCCTC ACGCTCCTCC ATCCTCACTG 
     1321 GCAAGTACGT CCACAACCAC AACACCTACA CCAACAATGA GAACTGCTCC TCGCCCTCCT 
     1381 GGCAGGCACA GCACGAGAGC CGCACCTTTG CCGTGTACCT CAATAGCACT GGCTACCGGA 
     1441 CAGCTTTCTT CGGGAAGTAT CTTAATGAAT ACAACGGCTC CTACGTGCCA CCCGGCTGGA 
     1501 AGGAGTGGGT CGGACTCCTT AAAAACTCCC GCTTTTATAA CTACACGCTG TGTCGGAACG 
     1561 GGGTGAAAGA GAAGCACGGC TCCGACTACT CCAAGGATTA CCTCACAGAC CTCATCACCA 
     1621 ATGACAGCGT GAGCTTCTTC CGCACGTCCA AGAAGATGTA CCCGCACAGG CCAGTCCTCA 
     1681 TGGTCATCAG CCATGCAGCC CCCCACGGCC CTGAGGATTC AGCCCCACAA TATTCACGCC 
     1741 TCTTCCCAAA CGCATCTCAG CACATCACGC CGAGCTACAA CTACGCGCCC AACCCGGACA 
     1801 AACACTGGAT CATGCGCTAC ACGGGGCCCA TGAAGCCCAT CCACATGGAA TTCACCAACA 
     1861 TGCTCCAGCG GAAGCGCTTG CAGACCCTCA TGTCGGTGGA CGACTCCATG GAGACGATTT 
     1921 ACAACATGCT GGTTGAGACG GGCGAGCTGG ACAACACGTA CATCGTATAC ACCGCCGACC 
     1981 ACGGTTACCA CATCGGCCAG TTTGGCCTGG TGAAAGGGAA ATCCATGCCA TATGAGTTTG 
     2041 ACATCAGGGT CCCGTTCTAC GTGAGGGGCC CCAACGTGGA AGCCGGCTGT CTGAATCCCC 
     2101 ACATCGTCCT CAACATTGAC CTGGCCCCCA CCATCCTGGA CATTGCAGGC CTGGACATAC 
     2161 CTGCGGATAT GGACGGGAAA TCCATCCTCA AGCTGCTGGA CACGGAGCGG CCGGTGAATC 
     2221 GGTTTCACTT GAAAAAGAAG ATGAGGGTCT GGCGGGACTC CTTCTTGGTG GAGAGAGGCA 
     2281 AGCTGCTACA CAAGAGAGAC AATGACAAGG TGGACGCCCA GGAGGAGAAC TTTCTGCCCA 
     2341 AGTACCAGCG TGTGAAGGAC CTGTGTCAGC GTGCTGAGTA CCAGACGGCG TGTGAGCAGC 
     2401 TGGGACAGAA GTGGCAGTGT GTGGAGGACG CCACGGGGAA GCTGAAGCTG CATAAGTGCA 
     2461 AGGGCCCCAT GCGGCTGGGC GGCAGCAGAG CCCTCTCCAA CCTCGTGCCC AAGTACTACG 
     2521 GGCAGGGCAG CGAGGCCTGC ACCTGTGACA GCGGGGACTA CAAGCTCAGC CTGGCCGGAC 
     2581 GCCGGAAAAA ACTCTTCAAG AAGAAGTACA AGGCCAGCTA TGTCCGCAGT CGCTCCATCC 
     2641 GCTCAGTGGC CATCGAGGTG GACGGCAGGG TGTACCACGT AGGCCTGGGT GATGCCGCCC 
     2701 AGCCCCGAAA CCTCACCAAG CGGCACTGGC CAGGGGCCCC TGAGGACCAA GATGACAAGG 
     2761 ATGGTGGGGA CTTCAGTGGC ACTGGAGGCC TTCCCGACTA CTCAGCCGCC AACCCCATTA 
     2821 AAGTGACACA TCGGTGCTAC ATCCTAGAGA ACGACACAGT CCAGTGTGAC CTGGACCTGT 
     2881 ACAAGTCCCT GCAGGCCTGG AAAGACCACA AGCTGCACAT CGACCACGAG ATTGAAACCC 
     2941 TGCAGAACAA AATTAAGAAC CTGAGGGAAG TCCGAGGTCA CCTGAAGAAA AAGCGGCCAG 
     3001 AAGAATGTGA CTGTCACAAA ATCAGCTACC ACACCCAGCA CAAAGGCCGC CTCAAGCACA 
     3061 GAGGCTCCAG TCTGCATCCT TTCAGGAAGG GCCTGCAAGA GAAGGACAAG GTGTGGCTGT 
     3121 TGCGGGAGCA GAAGCGCAAG AAGAAACTCC GCAAGCTGCT CAAGCGCCTG CAGAACAACG 
     3181 ACACGTGCAG CATGCCAGGC CTCACGTGCT TCACCCACGA CAACCAGCAC TGGCAGACGG 
     3241 CGCCTTTCTG GACACTGGGG CCTTTCTGTG CCTGCACCAG CGCCAACAAT AACACGTACT 
     3301 GGTGCATGAG GACCATCAAT GAGACTCACA ATTTCCTCTT CTGTGAATTT GCAACTGGCT 
     3361 TCCTAGAGTA CTTTGATCTC AACACAGACC CCTACCAGCT GATGAATGCA GTGAACACAC 
     3421 TGGACAGGGA TGTCCTCAAC CAGCTACACG TACAGCTCAT GGAGCTGAGG AGCTGCAAGG 
     3481 GTTACAAGCA GTGTAACCCC CGGACTCGAA ACATGGACCT GGGACTTAAA GATGGAGGAA 
     3541 GCTATGAGCA ATACAGGCAG TTTCAGCGTC GAAAGTGGCC AGAAATGAAG AGACCTTCTT 
     3601 CCAAATCACT GGGACAACTG TGGGAAGGCT GGGAAGGTAC GCGTACGCGG CCGCTCGAGA 
     3661 TGGAGAGCGA CGAGAGCGGC CTGCCCGCCA TGGAGATCGA GTGCCGCATC ACCGGCACCC 
     3721 TGAACGGCGT GGAGTTCGAG CTGGTGGGCG GCGGAGAGGG CACCCCCGAG CAGGGCCGCA 
     3781 TGACCAACAA GATGAAGAGC ACCAAAGGCG CCCTGACCTT CAGCCCCTAC CTGCTGAGCC 
     3841 ACGTGATGGG CTACGGCTTC TACCACTTCG GCACCTACCC CAGCGGCTAC GAGAACCCCT 
     3901 TCCTGCACGC CATCAACAAC GGCGGCTACA CCAACACCCG CATCGAGAAG TACGAGGACG 
     3961 GCGGCGTGCT GCACGTGAGC TTCAGCTACC GCTACGAGGC CGGCCGCGTG ATCGGCGACT 
     4021 TCAAGGTGAT GGGCACCGGC TTCCCCGAGG ACAGCGTGAT CTTCACCGAC AAGATCATCC 
     4081 GCAGCAACGC CACCGTGGAG CACCTGCACC CCATGGGCGA TAACGATCTG GATGGCAGCT 
     4141 TCACCCGCAC CTTCAGCCTG CGCGACGGCG GCTACTACAG CTCCGTGGTG GACAGCCACA 
     4201 TGCACTTCAA GAGCGCCATC CACCCCAGCA TCCTGCAGAA CGGGGGCCCC ATGTTCGCCT 
     4261 TCCGCCGCGT GGAGGAGGAT CACAGCAACA CCGAGCTGGG CATCGTGGAG TACCAGCACG 
     4321 CCTTCAAGAC CCCGGATGCA GATGCCGGTG AAGAAAGAGT TTAAACGGCC GGCCGCGGTC 
     4381 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4441 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4501 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4561 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4621 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4681 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4741 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     4801 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     4861 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     4921 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     4981 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5041 GCTTGGCTGT GGAATGTGTG TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC 
     5101 AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC 
     5161 TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG 
     5221 CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT 
     5281 GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCGGCCTC TGAGCTATTC 
     5341 CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT 
     5401 TGTATATCCA TTTTCGGATC TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA 
     5461 CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC 
     5521 TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG 
     5581 CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG 
     5641 GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT 
     5701 GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG 
     5761 TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG 
     5821 CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA 
     5881 GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG 
     5941 GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT 
     6001 CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT 
     6061 TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCCGACC GCTATCAGGA CATAGCGTTG 
     6121 GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT 
     6181 TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC 
     6241 TTCTGAGCGG GACTCTGGGG TTCGAAATGA CCGACCAAGC GACGCCCAAC CTGCCATCAC 
     6301 GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTCCGGG 
     6361 ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT GGAGTTCTTC GCCCACCCCA 
     6421 ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA 
     6481 ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT 
     6541 ATCATGTCTG TATACCGTCG ACCTCTAGCT AGAGCTTGGC GTAATCATGG TCATAGCTGT 
     6601 TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA 
     6661 AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC 
     6721 TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG 
     6781 CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC 
     6841 GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT 
     6901 CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA 
     6961 GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC 
     7021 ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC 
     7081 AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG 
     7141 GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA 
     7201 GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG 
     7261 TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC 
     7321 ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG 
     7381 GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT 
     7441 TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT 
     7501 CCGGCAAACA AACCACCGCT GGTAGCGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA 
     7561 GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA 
     7621 ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA 
     7681 TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT 
     7741 CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT 
     7801 CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT 
     7861 CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG 
     7921 CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT 
     7981 CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT 
     8041 TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG 
     8101 CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA 
     8161 AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT 
     8221 TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT 
     8281 GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC 
     8341 CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA 
     8401 AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT 
     8461 TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT 
     8521 TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA 
     8581 GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT 
     8641 ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA 
     8701 TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT 
     8761 TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG 
     8821 CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC 
     8881 AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC 
     8941 CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT 
     9001 TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA 
     9061 CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG 
     9121 CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//