LOCUS       RG229028                9092 bp    DNA     circular  SYN 
DEFINITION  PPP6R3 (tGFP-tagged) - Human protein phosphatase 6, regulatory subunit 3 (PPP6R3), transcript variant 2
ACCESSION   NM_001164163
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3573..4277 
                     /label="GFP"  
     misc_feature    4324..4909 
                     /label="PolyA signal"  
     misc_feature    7001..7646 
                     /label="ColE1"  
     misc_feature    5363..6157 
                     /label="Neo"  
     misc_feature    4994..5296 
                     /label="SV40 ori"  
     misc_feature    7661..8521 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3552..3558 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3552 
                     /label="PPP6R3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTTTGGAAA TTTGATCTTC ACTCATCATC CCACATAGAC ACACTTCTAG 
     1081 AAAGAGAAGA TGTAACACTG AAGGAGTTAA TGGATGAGGA AGATGTTTTA CAGGAATGTA 
     1141 AAGCTCAGAA CCGCAAACTT ATAGAGTTTC TGTTAAAAGC AGAATGTCTC GAAGATTTAG 
     1201 TCTCATTCAT TATAGAAGAA CCACCTCAAG ACATGGATGA AAAGATCAGA TACAAGTATC 
     1261 CAAATATATC TTGTGAGTTG CTCACTTCTG ATGTCTCCCA GATGAATGAT AGACTGGGAG 
     1321 AAGATGAATC CTTGCTAATG AAATTATATA GCTTCCTCCT AAACGATTCC CCTTTGAATC 
     1381 CACTACTTGC CAGTTTCTTC AGCAAGGTGC TAAGTATTCT TATCAGCAGA AAACCAGAAC 
     1441 AGATTGTGGA TTTCTTAAAG AAGAAGCATG ATTTTGTAGA CCTTATTATA AAGCACATAG 
     1501 GAACTTCTGC TATCATGGAT TTGTTGCTCA GGCTCCTGAC GTGTATCGAA CCTCCACAGC 
     1561 CCAGGCAAGA TGTGCTGAAT TGGTTAAATG AGGAGAAAAT TATCCAGAGG CTTGTGGAAA 
     1621 TAGTTCATCC ATCGCAAGAA GAAGATCGAC ATTCAAATGC ATCACAATCA CTTTGTGAAA 
     1681 TTGTTCGCCT GAGCAGAGAC CAGATGTTAC AAATTCAGAA CAGTACAGAG CCCGACCCCC 
     1741 TGCTTGCCAC TCTAGAAAAG CAAGAAATTA TAGAGCAGCT TCTATCAAAT ATTTTCCACA 
     1801 AGGAGAAAAA TGAGTCAGCC ATAGTCAGTG CAATCCAGAT ATTGCTGACT TTACTTGAGA 
     1861 CACGACGACC AACATTTGAA GGCCATATAG AGATCTGCCC ACCAGGCATG AGCCATTCAG 
     1921 CTTGTTCAGT AAACAAGAGT GTTCTAGAAG CCATCAGAGG AAGACTTGGA TCTTTTCATG 
     1981 AACTCCTGCT GGAGCCACCC AAGAAAAGTG TGATGAAGAC CACATGGGGT GTGCTGGATC 
     2041 CTCCTGTGGG GAATACCCGG TTGAATGTCA TTAGGTTGAT ATCCAGCCTG CTTCAAACCA 
     2101 ATACCAGCAG TATAAATGGG GACCTTATGG AGCTGAATAG CATTGGAGTC ATATTGAACA 
     2161 TGTTCTTCAA GTATACATGG AATAACTTTT TGCATACACA AGTGGAAATT TGTATTGCAC 
     2221 TGATTCTTGC AAGTCCTTTT GAAAACACAG AAAATGCCAC AATTACCGAT CAAGACTCCA 
     2281 CTGGTGATAA TTTGTTATTA AAACATCTTT TCCAAAAATG TCAATTAATA GAACGAATAC 
     2341 TTGAAGCCTG GGAAATGAAT GAGAAGAAAC AGGCTGAGGG AGGAAGACGG CATGGTTACA 
     2401 TGGGACACCT AACGAGGATA GCTAACTGTA TCGTGCACAG CACTGACAAG GGCCCCAACA 
     2461 GTGCATTAGT GCAGCAGCTT ATCAAAGATC TTCCCGACGA AGTCAGGGAA CGATGGGAGA 
     2521 CGTTCTGCAC AAGCTCCTTA GGAGAAACTA ACAAGAGGAA CACGGTAGAT CTAGCCTTTT 
     2581 CTGATTATCA GATGCAACAA ATGACGTCCA ATTTTATTGA CCAGTTTGGC TTCAACGATG 
     2641 AGAAGTTTGC AGATCAAGAT GACATTGGCA ATGTTTCTTT TGATCGAGTA TCAGACATCA 
     2701 ACTTTACTCT CAATACAAAT GAAAGTGGAA ATATTGCCTT GTTTGAAGCA TGTTGTAAGG 
     2761 AAAGAATACA ACAGTTTGAT GATGGTGGCT CTGATGAGGA AGATATATGG GAGGAAAAGC 
     2821 ACATCGCATT CACACCAGAA TCCCAAAGAC GATCCAGCTC GGGGAGTACA GACAGTGAGG 
     2881 AAAGTACAGA CTCTGAAGAA GAAGATGGAG CAAAGCAAGA CTTGTTTGAA CCCAGCAGTG 
     2941 CCAACACGGA GGATAAAATG GAGGTGGACC TGAGTGAACC ACCCAACTGG TCAGCTAACT 
     3001 TTGATGTCCC AATGGAAACA ACCCACGGTG CTCCATTGGA TTCTGTGGGA TCTGATGTCT 
     3061 GGAGCACAGA GGAGCCGATG CCAACTAAAG AGACGGGCTG GGCTTCTTTT TCAGAGTTCA 
     3121 CGTCTTCCCT GAGCACAAAA GATTCTTTAA GGAGTAATTC TCCAGTGGAA ATGGAAACCA 
     3181 GCACTGAACC CATGGACCCT CTGACTCCCA GTGCGGCTGC CCTGGCAGTG CAGCCAGAAG 
     3241 CGGCAGGCAG TGTGGCCATG GAAGCCAGCT CTGACGGAGA GGAGGATGCA GAAAGTACAG 
     3301 ACAAGGTAAC TGAGACAGTG ATGAATGGCG GCATGAAGGA AACGCTCAGC CTCACTGTAG 
     3361 ATGCCAAGAC AGAGACTGCG GTCTTCAAAA GTGAGGAAGG GAAACTGTCT ACCTCTCAAG 
     3421 ATGCTGCTTG TAAAGACGCA GAGGAGTGTC CCGAGACTGC AGAGGCGAAG TGCGCGGCGC 
     3481 CCAGGCCTCC CAGCAGCAGT CCCGAGCAGA GGACTGGCCA ACCAAGCGCA CCAGGTGACA 
     3541 CTTCAGTGAA TGGCCCTGTA ACGCGTACGC GGCCGCTCGA GATGGAGAGC GACGAGAGCG 
     3601 GCCTGCCCGC CATGGAGATC GAGTGCCGCA TCACCGGCAC CCTGAACGGC GTGGAGTTCG 
     3661 AGCTGGTGGG CGGCGGAGAG GGCACCCCCG AGCAGGGCCG CATGACCAAC AAGATGAAGA 
     3721 GCACCAAAGG CGCCCTGACC TTCAGCCCCT ACCTGCTGAG CCACGTGATG GGCTACGGCT 
     3781 TCTACCACTT CGGCACCTAC CCCAGCGGCT ACGAGAACCC CTTCCTGCAC GCCATCAACA 
     3841 ACGGCGGCTA CACCAACACC CGCATCGAGA AGTACGAGGA CGGCGGCGTG CTGCACGTGA 
     3901 GCTTCAGCTA CCGCTACGAG GCCGGCCGCG TGATCGGCGA CTTCAAGGTG ATGGGCACCG 
     3961 GCTTCCCCGA GGACAGCGTG ATCTTCACCG ACAAGATCAT CCGCAGCAAC GCCACCGTGG 
     4021 AGCACCTGCA CCCCATGGGC GATAACGATC TGGATGGCAG CTTCACCCGC ACCTTCAGCC 
     4081 TGCGCGACGG CGGCTACTAC AGCTCCGTGG TGGACAGCCA CATGCACTTC AAGAGCGCCA 
     4141 TCCACCCCAG CATCCTGCAG AACGGGGGCC CCATGTTCGC CTTCCGCCGC GTGGAGGAGG 
     4201 ATCACAGCAA CACCGAGCTG GGCATCGTGG AGTACCAGCA CGCCTTCAAG ACCCCGGATG 
     4261 CAGATGCCGG TGAAGAAAGA GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4321 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4381 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4441 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4501 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4561 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4621 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4681 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4741 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4801 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     4861 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     4921 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCTTGGCT GTGGAATGTG 
     4981 TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 
     5041 CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 
     5101 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC 
     5161 CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT 
     5221 ATTTATGCAG AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC 
     5281 TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA 
     5341 TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA 
     5401 GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC 
     5461 GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC 
     5521 AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG 
     5581 CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG 
     5641 GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT 
     5701 GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT 
     5761 ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA 
     5821 GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA 
     5881 CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC 
     5941 GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT 
     6001 GGCCGGCTGG GTGTGGCCGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT 
     6061 GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC 
     6121 GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG 
     6181 GGTTCGAAAT GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG 
     6241 CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC 
     6301 TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT 
     6361 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC 
     6421 TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT 
     6481 CGACCTCTAG CTAGAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT 
     6541 ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG 
     6601 CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG 
     6661 GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC 
     6721 GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC 
     6781 GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA 
     6841 ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG 
     6901 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT 
     6961 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA 
     7021 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC 
     7081 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT 
     7141 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG 
     7201 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG 
     7261 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT 
     7321 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC 
     7381 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG 
     7441 CTGGTAGCGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG 
     7501 AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG 
     7561 GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT 
     7621 GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT 
     7681 TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC 
     7741 TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA 
     7801 TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG 
     7861 GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT 
     7921 GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA 
     7981 TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT 
     8041 CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT 
     8101 TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG 
     8161 CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG 
     8221 AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG 
     8281 CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA 
     8341 AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT 
     8401 AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT 
     8461 GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT 
     8521 GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA 
     8581 TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT 
     8641 TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG 
     8701 TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT 
     8761 TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC 
     8821 TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG 
     8881 GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG 
     8941 AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT 
     9001 CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG 
     9061 AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//