LOCUS       RG229020                9017 bp    DNA     circular  SYN 
DEFINITION  PARP9 (tGFP-tagged) - Human poly (ADP-ribose) polymerase family, member 9 (PARP9), transcript variant 5
ACCESSION   NM_001146105
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3498..4202 
                     /label="GFP"  
     misc_feature    4249..4834 
                     /label="PolyA signal"  
     misc_feature    6926..7571 
                     /label="ColE1"  
     misc_feature    5288..6082 
                     /label="Neo"  
     misc_feature    4919..5221 
                     /label="SV40 ori"  
     misc_feature    7586..8446 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3477..3483 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3477 
                     /label="PARP9"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACTTTTCC ATGGTGGCCG GAGCAGCAGC TTACAATGAA AAATCAGAGA 
     1081 CTGGTGCTCT TGGAGAAAAC TATAGTTGGC AAATTCCCAT TAACCACAAT GACTTCAAAA 
     1141 TTTTAAAAAA TAATGAGCGT CAGCTGTGTG AAGTCCTCCA GAATAAGTTT GGCTGTATCT 
     1201 CTACCCTGGT CTCTCCAGTT CAGGAAGGCA ACAGCAAATC TCTGCAAGTG TTCAGAAAAA 
     1261 TGCTGACTCC TAGGATAGAG TTATCAGTCT GGAAAGATGA CCTCACCACA CATGCTGTTG 
     1321 ATGCTGTGGT GAATGCAGCC AATGAAGATC TTCTGCATGG GGGAGGCCTG GCCCTGGCCC 
     1381 TGGTAAAAGC TGGTGGATTT GAAATCCAAG AAGAGAGCAA ACAGTTTGTT GCCAGATATG 
     1441 GTAAAGTGTC AGCTGGTGAG ATAGCTGTCA CGGGAGCAGG GAGGCTTCCC TGCAAACAGA 
     1501 TCATCCATGC TGTTGGGCCT CGGTGGATGG AATGGGATAA ACAGGGATGT ACTGGAAAGC 
     1561 TGCAGAGGGC CATTGTAAGT ATTCTGAATT ATGTCATCTA TAAAAATACT CACATTAAGA 
     1621 CAGTAGCAAT TCCAGCCTTG AGCTCTGGGA TTTTTCAGTT CCCTCTGAAT TTGTGTACAA 
     1681 AGACTATTGT AGAGACTATC CGGGTTAGTT TGCAAGGGAA GCCAATGATG AGTAATTTGA 
     1741 AAGAAATTCA CCTGGTGAGC AATGAGGACC CTACTGTTGC TGCCTTTAAA GCTGCTTCAG 
     1801 AATTCATCCT AGGGAAGAGT GAGCTGGGAC AAGAAACCAC CCCTTCTTTC AATGCAATGG 
     1861 TCGTGAACAA CCTGACCCTC CAGATTGTCC AGGGCCACAT TGAATGGCAG ACGGCAGATG 
     1921 TAATTGTTAA TTCTGTAAAC CCACATGATA TTACAGTTGG ACCTGTGGCA AAGTCAATTC 
     1981 TACAACAAGC AGGAGTTGAA ATGAAATCGG AATTTCTTGC CACAAAGGCT AAACAGTTTC 
     2041 AACGGTCCCA GTTGGTACTG GTCACAAAAG GATTTAACTT GTTCTGTAAA TATATATACC 
     2101 ATGTACTGTG GCATTCAGAA TTTCCTAAAC CTCAGATATT AAAACATGCA ATGAAGGAGT 
     2161 GTTTGGAAAA ATGCATTGAG CAAAATATAA CTTCCATTTC CTTTCCTGCC CTTGGGACTG 
     2221 GAAACATGGA AATAAAGAAG GAAACAGCAG CAGAGATTTT GTTTGATGAA GTTTTAACAT 
     2281 TTGCCAAAGA CCATGTAAAA CACCAGTTAA CTGTAAAATT TGTGATCTTT CCAACAGATT 
     2341 TGGAGATATA TAAGGCTTTC AGTTCTGAAA TGGCAAAGAG GTCCAAGATG CTGAGTTTGA 
     2401 ACAATTACAG TGTCCCCCAG TCAACCAGAG AGGAGAAAAG AGAAAATGGG CTTGAAGCTA 
     2461 GATCTCCTGC CATCAATCTG ATGGGATTCA ACGTGGAAGA GATGTATGAG GCCCACGCAT 
     2521 GGATCCAAAG AATCCTGAGT CTCCAGAACC ACCACATCAT TGAGAATAAT CATATTCTGT 
     2581 ACCTTGGGAG AAAGGAACAT GACATTTTGT CTCAGCTTCA GAAAACTTCA AGTGTCTCCA 
     2641 TCACAGAAAT TATCAGCCCA GGAAGGACAG AGTTAGAGAT TGAAGGAGCC CGGGCTGACC 
     2701 TCATTGAGGT GGTTATGAAC ATTGAAGATA TGCTTTGTAA AGTACAGGAG GAAATGGCAA 
     2761 GGAAAAAGGA GCGAGGCCTT TGGCGCTCGT TAGGACAGTG GACTATTCAG CAACAAAAAA 
     2821 CCCAAGACGA AATGAAAGAA AATATCATAT TTCTGAAATG TCCTGTGCCT CCAACTCAAG 
     2881 AGCTTCTAGA TCAAAAGAAA CAGTTTGAAA AATGTGGTTT GCAGGTTCTA AAGGTGGAGA 
     2941 AGATAGACAA TGAGGTCCTT ATGGCTGCCT TTCAAAGAAA GAAGAAAATG ATGGAAGAAA 
     3001 AACTGCACAG GCAACCTGTG AGCCATAGGC TGTTTCAGCA AGTCCCATAC CAGTTCTGCA 
     3061 ATGTGGTATG CAGAGTTGGC TTTCAAAGAA TGTACTCGAC ACCTTGCGAT CCAAAATACG 
     3121 GAGCTGGCAT ATACTTCACC AAGAACCTCA AAAACCTGGC AGAGAAGGCC AAGAAAATCT 
     3181 CTGCTGCAGA TAAGCTGATC TATGTGTTTG AGGCTGAAGT ACTCACAGGC TTCTTCTGCC 
     3241 AGGGACATCC GTTAAATATT GTTCCCCCAC CACTGAGTCC TGGAGCTATA GATGGTCATG 
     3301 ACAGTGTGGT TGACAATGTC TCCAGCCCTG AAACCTTTGT TATTTTTAGT GGCATGCAGG 
     3361 CTATACCTCA GTATTTGTGG ACATGCACCC AGGAATATGT ACAGTCACAA GATTACTCAT 
     3421 CAGGACCAAT GAGACCCTTT GCACAGCATC CTTGGAGGGG ATTCGCAAGT GGCAGCCCTG 
     3481 TTGATACGCG TACGCGGCCG CTCGAGATGG AGAGCGACGA GAGCGGCCTG CCCGCCATGG 
     3541 AGATCGAGTG CCGCATCACC GGCACCCTGA ACGGCGTGGA GTTCGAGCTG GTGGGCGGCG 
     3601 GAGAGGGCAC CCCCGAGCAG GGCCGCATGA CCAACAAGAT GAAGAGCACC AAAGGCGCCC 
     3661 TGACCTTCAG CCCCTACCTG CTGAGCCACG TGATGGGCTA CGGCTTCTAC CACTTCGGCA 
     3721 CCTACCCCAG CGGCTACGAG AACCCCTTCC TGCACGCCAT CAACAACGGC GGCTACACCA 
     3781 ACACCCGCAT CGAGAAGTAC GAGGACGGCG GCGTGCTGCA CGTGAGCTTC AGCTACCGCT 
     3841 ACGAGGCCGG CCGCGTGATC GGCGACTTCA AGGTGATGGG CACCGGCTTC CCCGAGGACA 
     3901 GCGTGATCTT CACCGACAAG ATCATCCGCA GCAACGCCAC CGTGGAGCAC CTGCACCCCA 
     3961 TGGGCGATAA CGATCTGGAT GGCAGCTTCA CCCGCACCTT CAGCCTGCGC GACGGCGGCT 
     4021 ACTACAGCTC CGTGGTGGAC AGCCACATGC ACTTCAAGAG CGCCATCCAC CCCAGCATCC 
     4081 TGCAGAACGG GGGCCCCATG TTCGCCTTCC GCCGCGTGGA GGAGGATCAC AGCAACACCG 
     4141 AGCTGGGCAT CGTGGAGTAC CAGCACGCCT TCAAGACCCC GGATGCAGAT GCCGGTGAAG 
     4201 AAAGAGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4261 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4321 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4381 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4441 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4501 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4561 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4621 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4681 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     4741 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     4801 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4861 ATGCCTGTTG AATTGGGTAC GCGGCCAGCT TGGCTGTGGA ATGTGTGTCA GTTAGGGTGT 
     4921 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA 
     4981 GCAACCAGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 
     5041 CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG 
     5101 CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC 
     5161 GAGGCCGCCT CGGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA 
     5221 GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA 
     5281 GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT 
     5341 TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC 
     5401 GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC 
     5461 GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC 
     5521 GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG 
     5581 GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC 
     5641 ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC 
     5701 CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT 
     5761 CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC 
     5821 AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG 
     5881 AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG 
     5941 GCCGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC 
     6001 GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC 
     6061 GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GAAATGACCG 
     6121 ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA 
     6181 GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC 
     6241 TCATGCTGGA GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT 
     6301 AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG 
     6361 GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA 
     6421 GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC 
     6481 CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT 
     6541 AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC 
     6601 AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT 
     6661 CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG 
     6721 CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA 
     6781 TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT 
     6841 TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC 
     6901 GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT 
     6961 CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG 
     7021 TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA 
     7081 AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT 
     7141 ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA 
     7201 ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA 
     7261 ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT 
     7321 TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTTTTT 
     7381 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT 
     7441 TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA 
     7501 GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA 
     7561 TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC 
     7621 CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA 
     7681 TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC 
     7741 CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA 
     7801 GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA 
     7861 GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG 
     7921 TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC 
     7981 GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG 
     8041 TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT 
     8101 CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT 
     8161 CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA 
     8221 ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC 
     8281 GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC 
     8341 CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA 
     8401 GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT 
     8461 TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT 
     8521 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC 
     8581 CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG 
     8641 TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC 
     8701 TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC 
     8761 GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA 
     8821 GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA 
     8881 ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG 
     8941 ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA 
     9001 AATTTAACGC GAATTTT
//