LOCUS       RG228911                9617 bp    DNA     circular  SYN 
DEFINITION  IDE (tGFP-tagged) - Human insulin-degrading enzyme (IDE), transcript variant 2
ACCESSION   NM_001165946
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4098..4802 
                     /label="GFP"  
     misc_feature    4849..5434 
                     /label="PolyA signal"  
     misc_feature    7526..8171 
                     /label="ColE1"  
     misc_feature    5888..6682 
                     /label="Neo"  
     misc_feature    5519..5821 
                     /label="SV40 ori"  
     misc_feature    8186..9046 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4077..4083 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4077 
                     /label="IDE"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGGTACCGG CTAGCGTGGC TTCTGCACCC CGCACTGCCC AGCACCTTCC 
     1081 GCTCAGTCCT CGGCGCCCGC CTGCCGCCTC CGGAGCGCCT GTGTGGTTTC CAAAAAAAGA 
     1141 CTTACAGCAA AATGAATAAT CCAGCCATCA AGAGAATAGG AAATCACATT ACCAAGTCTC 
     1201 CTGAAGACAA GCGAGAATAT CGAGGGCTAG AGCTGGCCAA TGGTATCAAA GTACTTCTTA 
     1261 TCAGTGATCC CACCACGGAT AAGTCATCAG CAGCACTTGA TGTGCACATA GGTTCATTGT 
     1321 CGGATCCTCC AAATATTGCT GGCTTAAGTC ATTTTTGTGA ACATATGCTT TTTTTGGGAA 
     1381 CAAAGAAATA CCCTAAAGAA AATGAATACA GCCAGTTTCT CAGTGAGCAT GCAGGAAGTT 
     1441 CAAATGCCTT TACTAGTGGA GAGCATACCA ATTACTATTT TGATGTTTCT CATGAACACC 
     1501 TAGAAGGTGC CCTAGACAGG TTTGCACAGT TTTTTCTGTG CCCCTTGTTC GATGAAAGTT 
     1561 GCAAAGACAG AGAGGTGAAT GCAGTTGATT CAGAACATGA GAAGAATGTG ATGAATGATG 
     1621 CCTGGAGACT CTTTCAATTG GAAAAAGCTA CAGGGAATCC TAAACACCCC TTCAGTAAAT 
     1681 TTGGGACAGG TAACAAATAT ACTCTGGAGA CTAGACCAAA CCAAGAAGGC ATTGATGTAA 
     1741 GACAAGAGCT ACTGAAATTC CATTCTGCTT ACTATTCATC CAACTTAATG GCTGTTTGTG 
     1801 TTTTAGGTCG AGAATCTTTA GATGACTTGA CTAATCTGGT GGTAAAGTTA TTTTCTGAAG 
     1861 TAGAGAACAA AAATGTTCCA TTGCCAGAAT TTCCTGAACA CCCTTTCCAA GAAGAACATC 
     1921 TTAAACAACT TTACAAAATA GTACCCATTA AAGATATTAG GAATCTCTAT GTGACATTTC 
     1981 CCATACCTGA CCTTCAGAAA TACTACAAAT CAAATCCTGG TCATTATCTT GGTCATCTCA 
     2041 TTGGGCATGA AGGTCCTGGA AGTCTGTTAT CAGAACTTAA GTCAAAGGGC TGGGTTAATA 
     2101 CTCTTGTTGG TGGGCAGAAG GAAGGAGCCC GAGGTTTTAT GTTTTTTATC ATTAATGTGG 
     2161 ACTTGACCGA GGAAGGATTA TTACATGTTG AAGATATAAT TTTGCACATG TTTCAATACA 
     2221 TTCAGAAGTT ACGTGCAGAA GGACCTCAAG AATGGGTTTT CCAAGAGTGC AAGGACTTGA 
     2281 ATGCTGTTGC TTTTAGGTTT AAAGACAAAG AGAGGCCACG GGGCTATACA TCTAAGATTG 
     2341 CAGGAATATT GCATTATTAT CCCCTAGAAG AGGTGCTCAC AGCGGAATAT TTACTGGAAG 
     2401 AATTTAGACC TGACTTAATA GAGATGGTTC TCGATAAACT CAGACCAGAA AATGTCCGGG 
     2461 TTGCCATAGT TTCTAAATCT TTTGAAGGAA AAACTGATCG CACAGAAGAG TGGTATGGAA 
     2521 CCCAGTACAA ACAAGAAGCT ATACCGGATG AAGTCATCAA GAAATGGCAA AATGCTGACC 
     2581 TGAATGGGAA ATTTAAACTT CCTACAAAGA ATGAATTTAT TCCTACGAAT TTTGAGATTT 
     2641 TACCGTTAGA AAAAGAGGCG ACACCATACC CTGCTCTTAT TAAGGATACA GCTATGAGCA 
     2701 AACTTTGGTT CAAACAAGAT GATAAGTTTT TTTTGCCGAA GGCTTGTCTC AACTTTGAAT 
     2761 TTTTCAGCCC ATTTGCTTAT GTGGACCCCT TGCACTGTAA CATGGCCTAT TTGTACCTTG 
     2821 AGCTCCTCAA AGACTCACTC AACGAGTATG CATATGCAGC AGAGCTAGCA GGCTTGAGCT 
     2881 ATGATCTCCA AAATACCATC TATGGGATGT ATCTTTCAGT GAAAGGTTAC AATGACAAGC 
     2941 AGCCAATTTT ACTAAAGAAG ATTATTGAGA AAATGGCTAC CTTTGAGATT GATGAAAAAA 
     3001 GATTTGAAAT TATCAAAGAA GCATATATGC GATCTCTTAA CAATTTCCGG GCTGAACAGC 
     3061 CTCACCAGCA TGCCATGTAC TACCTCCGCT TGCTGATGAC TGAAGTGGCC TGGACTAAAG 
     3121 ATGAGTTAAA AGAAGCTCTG GATGATGTAA CCCTTCCTCG CCTTAAGGCC TTCATACCTC 
     3181 AGCTCCTGTC ACGGCTGCAC ATTGAAGCCC TTCTCCATGG AAACATAACA AAGCAGGCTG 
     3241 CATTAGGAAT TATGCAGATG GTTGAAGACA CCCTCATTGA ACATGCTCAT ACCAAACCTC 
     3301 TCCTTCCAAG TCAGCTGGTT CGGTATAGAG AAGTTCAGCT CCCTGACAGA GGATGGTTTG 
     3361 TTTATCAGCA GAGAAATGAA GTTCACAATA ACTGTGGCAT CGAGATATAC TACCAAACAG 
     3421 ACATGCAAAG CACCTCAGAG AATATGTTTC TGGAGCTCTT CTGTCAGATT ATCTCGGAAC 
     3481 CTTGCTTCAA CACCCTGCGC ACCAAGGAGC AGTTGGGCTA TATCGTCTTC AGCGGGCCAC 
     3541 GTCGAGCTAA TGGCATACAG GGCTTGAGAT TCATCATCCA GTCAGAAAAG CCACCTCACT 
     3601 ACCTAGAAAG CAGAGTGGAA GCTTTCTTAA TTACCATGGA AAAGTCCATA GAGGACATGA 
     3661 CAGAAGAGGC CTTCCAAAAA CACATTCAGG CATTAGCAAT TCGTCGACTA GACAAACCAA 
     3721 AGAAGCTATC TGCTGAGTGT GCTAAATACT GGGGAGAAAT CATCTCCCAG CAATATAATT 
     3781 TTGACAGAGA TAACACTGAG GTTGCATATT TAAAGACACT TACCAAGGAA GATATCATCA 
     3841 AATTCTACAA GGAAATGTTG GCAGTAGATG CTCCAAGGAG ACATAAGGTA TCCGTCCATG 
     3901 TTCTTGCCAG GGAAATGGAT TCTTGTCCTG TTGTTGGAGA GTTCCCATGT CAAAATGACA 
     3961 TAAATTTGTC ACAAGCACCA GCCTTGCCAC AACCTGAAGT GATTCAGAAC ATGACCGAAT 
     4021 TCAAGCGTGG TCTGCCACTG TTTCCCCTTG TGAAACCACA TATTAACTTC ATGGCTGCAA 
     4081 AACTCACGCG TACGCGGCCG CTCGAGATGG AGAGCGACGA GAGCGGCCTG CCCGCCATGG 
     4141 AGATCGAGTG CCGCATCACC GGCACCCTGA ACGGCGTGGA GTTCGAGCTG GTGGGCGGCG 
     4201 GAGAGGGCAC CCCCGAGCAG GGCCGCATGA CCAACAAGAT GAAGAGCACC AAAGGCGCCC 
     4261 TGACCTTCAG CCCCTACCTG CTGAGCCACG TGATGGGCTA CGGCTTCTAC CACTTCGGCA 
     4321 CCTACCCCAG CGGCTACGAG AACCCCTTCC TGCACGCCAT CAACAACGGC GGCTACACCA 
     4381 ACACCCGCAT CGAGAAGTAC GAGGACGGCG GCGTGCTGCA CGTGAGCTTC AGCTACCGCT 
     4441 ACGAGGCCGG CCGCGTGATC GGCGACTTCA AGGTGATGGG CACCGGCTTC CCCGAGGACA 
     4501 GCGTGATCTT CACCGACAAG ATCATCCGCA GCAACGCCAC CGTGGAGCAC CTGCACCCCA 
     4561 TGGGCGATAA CGATCTGGAT GGCAGCTTCA CCCGCACCTT CAGCCTGCGC GACGGCGGCT 
     4621 ACTACAGCTC CGTGGTGGAC AGCCACATGC ACTTCAAGAG CGCCATCCAC CCCAGCATCC 
     4681 TGCAGAACGG GGGCCCCATG TTCGCCTTCC GCCGCGTGGA GGAGGATCAC AGCAACACCG 
     4741 AGCTGGGCAT CGTGGAGTAC CAGCACGCCT TCAAGACCCC GGATGCAGAT GCCGGTGAAG 
     4801 AAAGAGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4861 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4921 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4981 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     5041 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     5101 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     5161 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     5221 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     5281 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5341 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5401 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5461 ATGCCTGTTG AATTGGGTAC GCGGCCAGCT TGGCTGTGGA ATGTGTGTCA GTTAGGGTGT 
     5521 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA 
     5581 GCAACCAGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 
     5641 CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG 
     5701 CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC 
     5761 GAGGCCGCCT CGGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA 
     5821 GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA 
     5881 GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT 
     5941 TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC 
     6001 GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC 
     6061 GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC 
     6121 GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG 
     6181 GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC 
     6241 ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC 
     6301 CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT 
     6361 CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC 
     6421 AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG 
     6481 AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG 
     6541 GCCGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC 
     6601 GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC 
     6661 GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GAAATGACCG 
     6721 ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA 
     6781 GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC 
     6841 TCATGCTGGA GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT 
     6901 AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG 
     6961 GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA 
     7021 GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC 
     7081 CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT 
     7141 AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC 
     7201 AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT 
     7261 CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG 
     7321 CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA 
     7381 TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT 
     7441 TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC 
     7501 GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT 
     7561 CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG 
     7621 TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA 
     7681 AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT 
     7741 ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA 
     7801 ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA 
     7861 ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT 
     7921 TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTTTTT 
     7981 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT 
     8041 TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA 
     8101 GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA 
     8161 TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC 
     8221 CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA 
     8281 TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC 
     8341 CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA 
     8401 GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA 
     8461 GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG 
     8521 TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC 
     8581 GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG 
     8641 TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT 
     8701 CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT 
     8761 CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA 
     8821 ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC 
     8881 GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC 
     8941 CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA 
     9001 GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT 
     9061 TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT 
     9121 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC 
     9181 CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG 
     9241 TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC 
     9301 TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC 
     9361 GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA 
     9421 GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA 
     9481 ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG 
     9541 ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA 
     9601 AATTTAACGC GAATTTT
//