LOCUS       RG228608                9218 bp    DNA     circular  SYN 
DEFINITION  DISC1 (tGFP-tagged) - Human disrupted in schizophrenia 1 (DISC1), transcript variant a
ACCESSION   NM_001164537
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3699..4403 
                     /label="GFP"  
     misc_feature    4450..5035 
                     /label="PolyA signal"  
     misc_feature    7127..7772 
                     /label="ColE1"  
     misc_feature    5489..6283 
                     /label="Neo"  
     misc_feature    5120..5422 
                     /label="SV40 ori"  
     misc_feature    7787..8647 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3678..3684 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3678 
                     /label="DISC1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCAGGCGGG GGTCCTCAGG GCGCCCCAGC CGCCGCCGGC GGCGGCGGCG 
     1081 TGAGCCACCG CGCAGGCAGC CGGGATTGCT TACCACCTGC AGCGTGCTTT CGGAGGCGGC 
     1141 GGCTGGCACG GAGGCCGGGC TACATGAGAA GCTCGACAGG GCCTGGGATC GGGTTCCTTT 
     1201 CCCCAGCAGT GGGCACACTG TTCCGGTTCC CAGGAGGGGT GTCTGGCGAG GAGTCCCACC 
     1261 ACTCGGAGTC CAGGGCCAGA CAGTGTGGCC TTGACTCGAG AGGCCTCTTG GTCCGGAGCC 
     1321 CTGTTTCCAA GAGTGCAGCA GCCCCTACTG TGACCTCTGT GAGAGGAACC TCGGCGCACT 
     1381 TTGGGATTCA GCTCAGAGGT GGCACCAGAT TGCCTGACAG GCTTAGCTGG CCGTGTGGCC 
     1441 CTGGGAGTGC TGGGTGGCAG CAAGAGTTTG CAGCCATGGA TAGTTCTGAG ACCCTGGACG 
     1501 CCAGCTGGGA GGCAGCCTGC AGCGATGGAG CAAGGCGTGT CCGGGCAGCA GGCTCTCTGC 
     1561 CATCAGCAGA GTTGAGTAGC AACAGCTGCA GCCCTGGCTG TGGCCCTGAG GTCCCCCCAA 
     1621 CCCCTCCTGG CTCTCACAGT GCCTTTACCT CAAGCTTTAG CTTTATTCGG CTCTCGCTTG 
     1681 GCTCTGCCGG GGAACGTGGA GAAGCAGAAG GCTGCCCACC ATCCAGAGAG GCTGAGTCCC 
     1741 ATTGCCAGAG CCCCCAGGAG ATGGGAGCCA AAGCTGCCAG CTTGGACGGG CCTCACGAGG 
     1801 ACCCGCGATG TCTCTCTCGG CCCTTCAGTC TCTTGGCTAC ACGGGTCTCT GCAGACTTGG 
     1861 CCCAGGCCGC AAGGAACAGC TCCAGGCCAG AGCGTGACAT GCATTCTTTA CCAGACATGG 
     1921 ACCCTGGCTC CTCCAGTTCT CTGGATCCCT CACTGGCTGG CTGTGGTGGT GATGGGAGCA 
     1981 GCGGCTCAGG GGATGCCCAC TCTTGGGACA CCCTGCTCAG GAAATGGGAG CCAGTGCTGC 
     2041 GGGACTGCCT GCTGAGAAAC CGGAGGCAGA TGGAGGTAAT ATCCTTAAGA TTAAAACTTC 
     2101 AGAAACTTCA GGAAGATGCA GTTGAGAATG ATGATTATGA TAAAGTAGAG ACGGGGTTTC 
     2161 ACTATGTTGG TCAAGCTGGT CTTGAACTCC TGACCTCAAG CAATCCACCT GCTTCGGCCT 
     2221 CCCAAAGTGC TGGGATTACA GCTGAGACGT TACAACAAAG ATTAGAAGAC CTGGAACAAG 
     2281 AGAAAATCAG CCTGCACTTT CAACTTCCTT CAAGGCAGCC AGCTCTTAGC AGTTTCCTGG 
     2341 GTCACCTGGC AGCACAAGTC CAGGCTGCCT TGCGCCGTGG GGCCACTCAG CAGGCCAGCG 
     2401 GAGATGACAC CCACACCCCA CTGAGAATGG AGCCGAGGCT GTTGGAACCC ACTGCTCAGG 
     2461 ACAGCTTGCA CGTGTCCATC ACGAGACGAG ACTGGCTTCT TCAGGAAAAG CAGCAGCTAC 
     2521 AGAAAGAAAT CGAAGCTCTC CAAGCAAGGA TGTTTGTGCT GGAAGCCAAA GATCAACAGC 
     2581 TGAGAAGGGA AATAGAGGAG CAAGAGCAGC AACTCCAGTG GCAGGGCTGC GACCTGACCC 
     2641 CACTGGTGGG CCAGCTGTCC CTGGGTCAGC TGCAGGAGGT CAGCAAGGCC TTGCAGGACA 
     2701 CCCTGGCCTC AGCCGGTCAG ATTCCCTTCC ATGCAGAGCC ACCGGAAACC ATAAGGAGCC 
     2761 TCCAGGAAAG AATAAAATCC CTCAACTTGT CACTTAAAGA AATCACTACT AAGGTGTGTA 
     2821 TGAGTGAGAA ATTCTGCAGC ACCCTGAGGA AGAAAGTTAA CGATATTGAA ACCCAACTAC 
     2881 CAGCCTTGCT TGAAGCCAAA ATGCATGCCA TATCAGGAAA CCATTTCTGG ACGGCTAAAG 
     2941 ACCTCACCGA GGAGATTAGA TCATTAACAT CAGAGAGAGA AGGGCTGGAG GGACTCCTCA 
     3001 GCAAGCTGTT GGTGTTGAGT TCCAGGAATG TCAAAAAGCT GGGAAGTGTT AAAGAAGATT 
     3061 ACAACAGACT GAGAAGAGAA GTGGAGCACC AGGAGACTGC CTATGAAACA AGTGTGAAGG 
     3121 AAAATACTAT GAAGTACATG GAAACACTTA AGAATAAACT GTGCAGCTGC AAGTGTCCAC 
     3181 TGCTTGGGAA AGTGTGGGAA GCTGACTTGG AAGCTTGTCG ATTGCTTATC CAGAGCCTAC 
     3241 AGCTCCAGGA AGCCAGGGGA AGCCTGTCTG TAGAAGATGA GAGGCAGATG GATGACTTAG 
     3301 AGGGAGCTGC TCCTCCTATT CCCCCCAGGC TCCACTCCGA GGATAAAAGG AAGACCCCTT 
     3361 TGAAGGTATT GGAAGAATGG AAGACTCACC TCATCCCCTC TCTGCACTGT GCTGGAGGTG 
     3421 AACAGAAAGA GGAATCTTAC ATCCTTTCTG CAGAACTTGG AGAAAAGTGT GAAGACATAG 
     3481 GCAAGAAGCT ATTGTACTTG GAAGATCAAC TTCACACAGC AATCCACAGT CATGATGAAG 
     3541 ATCTCATTCA GTCTCTCAGG AGGGAGCTCC AGATGGTGAA GGAAACTCTG CAGGCCATGA 
     3601 TCCTGCAGCT CCAGCCAGCA AAGGAGGCGG GAGAAAGAGA AGCTGCAGCT TCCTGCATGA 
     3661 CAGCTGGTGT CCACGAAGCA CAAGCCACGC GTACGCGGCC GCTCGAGATG GAGAGCGACG 
     3721 AGAGCGGCCT GCCCGCCATG GAGATCGAGT GCCGCATCAC CGGCACCCTG AACGGCGTGG 
     3781 AGTTCGAGCT GGTGGGCGGC GGAGAGGGCA CCCCCGAGCA GGGCCGCATG ACCAACAAGA 
     3841 TGAAGAGCAC CAAAGGCGCC CTGACCTTCA GCCCCTACCT GCTGAGCCAC GTGATGGGCT 
     3901 ACGGCTTCTA CCACTTCGGC ACCTACCCCA GCGGCTACGA GAACCCCTTC CTGCACGCCA 
     3961 TCAACAACGG CGGCTACACC AACACCCGCA TCGAGAAGTA CGAGGACGGC GGCGTGCTGC 
     4021 ACGTGAGCTT CAGCTACCGC TACGAGGCCG GCCGCGTGAT CGGCGACTTC AAGGTGATGG 
     4081 GCACCGGCTT CCCCGAGGAC AGCGTGATCT TCACCGACAA GATCATCCGC AGCAACGCCA 
     4141 CCGTGGAGCA CCTGCACCCC ATGGGCGATA ACGATCTGGA TGGCAGCTTC ACCCGCACCT 
     4201 TCAGCCTGCG CGACGGCGGC TACTACAGCT CCGTGGTGGA CAGCCACATG CACTTCAAGA 
     4261 GCGCCATCCA CCCCAGCATC CTGCAGAACG GGGGCCCCAT GTTCGCCTTC CGCCGCGTGG 
     4321 AGGAGGATCA CAGCAACACC GAGCTGGGCA TCGTGGAGTA CCAGCACGCC TTCAAGACCC 
     4381 CGGATGCAGA TGCCGGTGAA GAAAGAGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4441 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4501 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4561 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4621 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4681 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4741 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4801 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     4861 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     4921 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     4981 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5041 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC TTGGCTGTGG 
     5101 AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA 
     5161 AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC 
     5221 AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG 
     5281 CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT 
     5341 TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG AGCTATTCCA GAAGTAGTGA 
     5401 GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT 
     5461 TTCGGATCTG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG 
     5521 CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG 
     5581 ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT 
     5641 TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA 
     5701 TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG 
     5761 GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT 
     5821 GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT 
     5881 CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG 
     5941 ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA 
     6001 GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC 
     6061 CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC 
     6121 GACTGTGGCC GGCTGGGTGT GGCCGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT 
     6181 ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC 
     6241 GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA 
     6301 CTCTGGGGTT CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA GATTTCGATT 
     6361 CCACCGCCGC CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA 
     6421 TGATCCTCCA GCGCGGGGAT CTCATGCTGG AGTTCTTCGC CCACCCCAAC TTGTTTATTG 
     6481 CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT 
     6541 TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGTA 
     6601 TACCGTCGAC CTCTAGCTAG AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA 
     6661 ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT 
     6721 GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC 
     6781 AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG 
     6841 GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC 
     6901 GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG 
     6961 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA 
     7021 AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC 
     7081 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC 
     7141 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG 
     7201 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT 
     7261 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC 
     7321 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC 
     7381 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG 
     7441 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG 
     7501 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA 
     7561 CCACCGCTGG TAGCGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT 
     7621 CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC 
     7681 GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT 
     7741 AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC 
     7801 AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG 
     7861 CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG 
     7921 CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC 
     7981 CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA 
     8041 TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG 
     8101 TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT 
     8161 CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA 
     8221 GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG 
     8281 TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA 
     8341 CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT 
     8401 GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA 
     8461 TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT 
     8521 CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT 
     8581 CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA 
     8641 AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT 
     8701 GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC 
     8761 GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG 
     8821 GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT 
     8881 TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC 
     8941 GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG 
     9001 ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA 
     9061 CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC 
     9121 CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA 
     9181 AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//