LOCUS       RG228564                8762 bp    DNA     circular  SYN 
DEFINITION  RHOBTB2 (tGFP-tagged) - Human Rho-related BTB domain containing 2 (RHOBTB2), transcript variant 2
ACCESSION   NM_001160037
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3243..3947 
                     /label="GFP"  
     misc_feature    3994..4579 
                     /label="PolyA signal"  
     misc_feature    6671..7316 
                     /label="ColE1"  
     misc_feature    5033..5827 
                     /label="Neo"  
     misc_feature    4664..4966 
                     /label="SV40 ori"  
     misc_feature    7331..8191 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3222..3228 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3222 
                     /label="RHOBTB2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAAGCGCGG TCCCGTTTAA TGGATTCTGA CATGGATTAT GAAAGGCCAA 
     1081 ACGTAGAGAC CATCAAGTGC GTTGTGGTGG GGGACAACGC CGTGGGTAAG ACCAGGCTCA 
     1141 TCTGTGCCCG CGCTTGCAAT GCCACCCTCA CCCAGTACCA GCTGCTGGCC ACGCATGTGC 
     1201 CCACAGTATG GGCCATCGAC CAATATCGTG TGTGCCAGGA GGTGCTGGAA CGCTCCCGAG 
     1261 ACGTGGTAGA TGATGTCAGC GTCTCTCTGC GCCTCTGGGA CACCTTTGGA GACCACCACA 
     1321 AAGACCGTCG CTTTGCTTAT GGGAGATCTG ATGTGGTGGT TCTGTGCTTC TCCATTGCCA 
     1381 ACCCCAATTC CCTCCACCAT GTCAAGACCA TGTGGTACCC AGAAATCAAG CACTTCTGCC 
     1441 CCCGAGCACC TGTCATCTTG GTGGGCTGCC AGTTGGACCT GCGCTACGCT GACCTGGAGG 
     1501 CTGTCAACAG GGCTAGGCGA CCCTTGGCTA GGCCCATCAA ACCTAATGAA ATCCTGCCCC 
     1561 CAGAGAAGGG TCGGGAGGTG GCCAAGGAGC TGGGCATCCC CTACTATGAG ACCAGCGTGG 
     1621 TGGCCCAGTT CGGCATCAAG GACGTCTTTG ACAACGCCAT CCGAGCTGCA CTCATCTCCC 
     1681 GCCGCCACCT GCAGTTCTGG AAGTCCCACC TCCGCAATGT GCAGCGGCCT CTGCTGCAGG 
     1741 CACCCTTCCT ACCCCCCAAG CCACCGCCCC CGATCATCGT GGTGCCCGAC CCTCCCTCCA 
     1801 GCAGCGAGGA GTGCCCCGCC CACCTCCTGG AGGACCCGCT CTGCGCGGAC GTCATCCTGG 
     1861 TGCTGCAGGA GCGGGTGCGC ATCTTTGCCC ACAAGATCTA CCTCTCCACC TCTTCCTCCA 
     1921 AGTTCTATGA CCTGTTCCTC ATGGACCTGA GTGAGGGGGA GCTGGGGGGC CCCTCGGAGC 
     1981 CAGGGGGCAC CCACCCAGAG GACCACCAGG GCCACTCTGA TCAACACCAC CACCATCACC 
     2041 ACCACCACCA TGGGCGAGAC TTCCTGCTCC GAGCAGCCAG CTTTGACGTG TGCGAGAGCG 
     2101 TGGATGAGGC TGGGGGCTCC GGTCCTGCTG GCCTCCGTGC TTCCACCAGC GACGGGATCT 
     2161 TACGGGGCAA CGGAACAGGG TACCTACCGG GCAGGGGTCG TGTGCTGTCT TCCTGGAGCC 
     2221 GAGCTTTTGT GAGCATCCAG GAAGAGATGG CAGAAGATCC TCTCACCTAC AAATCCCGGC 
     2281 TGATGGTGGT GGTGAAGATG GACAGTTCCA TCCAGCCGGG GCCCTTCCGG GCTGTCCTCA 
     2341 AGTACCTGTA CACGGGGGAG CTAGATGAGA ACGAGCGTGA CCTCATGCAC ATTGCCCACA 
     2401 TTGCTGAGCT GCTCGAGGTC TTTGATCTGC GCATGATGGT GGCCAACATT CTCAACAATG 
     2461 AGGCCTTCAT GAACCAGGAG ATCACCAAGG CCTTCCACGT CCGCCGGACC AACCGGGTTA 
     2521 AGGAGTGCTT GGCAAAAGGC ACCTTCTCAG ATGTGACCTT CATCCTGGAT GATGGCACCA 
     2581 TCAGCGCCCA CAAGCCCCTG TTGATTTCCA GCTGTGACTG GATGGCTGCC ATGTTTGGGG 
     2641 GGCCATTTGT GGAGAGCTCC ACCCGGGAGG TGGTGTTTCC CTACACAAGC AAGAGCTGCA 
     2701 TGCGGGCCGT GCTGGAATAC CTCTACACCG GCATGTTCAC CTCCAGCCCC GACCTGGATG 
     2761 ACATGAAGCT CATCATTCTA GCCAACCGCC TCTGCCTGCC ACACCTGGTT GCCCTCACAG 
     2821 AGCAGTACAC AGTGACCGGG CTGATGGAAG CGACCCAGAT GATGGTGGAC ATCGATGGGG 
     2881 ACGTCCTTGT GTTCCTGGAA CTGGCTCAGT TCCACTGTGC GTACCAGCTG GCCGACTGGT 
     2941 GTCTCCACCA CATCTGCACC AACTACAACA ACGTGTGCCG CAAGTTCCCC CGAGACATGA 
     3001 AGGCCATGTC CCCAGAAAAC CAGGAGTATT TCGAGAAGCA TCGGTGGCCA CCTGTGTGGT 
     3061 ACCTGAAGGA GGAAGATCAT TACCAGCGGG CACGGAAGGA GCGTGAGAAG GAGGACTACC 
     3121 TCCACCTCAA GCGGCAGCCC AAACGGCGTT GGCTCTTCTG GAACAGTCCA TCCTCCCCGT 
     3181 CTTCCTCGGC AGCCTCCTCC TCATCCCCAT CTTCCTCCTC GGCTGTGGTC ACGCGTACGC 
     3241 GGCCGCTCGA GATGGAGAGC GACGAGAGCG GCCTGCCCGC CATGGAGATC GAGTGCCGCA 
     3301 TCACCGGCAC CCTGAACGGC GTGGAGTTCG AGCTGGTGGG CGGCGGAGAG GGCACCCCCG 
     3361 AGCAGGGCCG CATGACCAAC AAGATGAAGA GCACCAAAGG CGCCCTGACC TTCAGCCCCT 
     3421 ACCTGCTGAG CCACGTGATG GGCTACGGCT TCTACCACTT CGGCACCTAC CCCAGCGGCT 
     3481 ACGAGAACCC CTTCCTGCAC GCCATCAACA ACGGCGGCTA CACCAACACC CGCATCGAGA 
     3541 AGTACGAGGA CGGCGGCGTG CTGCACGTGA GCTTCAGCTA CCGCTACGAG GCCGGCCGCG 
     3601 TGATCGGCGA CTTCAAGGTG ATGGGCACCG GCTTCCCCGA GGACAGCGTG ATCTTCACCG 
     3661 ACAAGATCAT CCGCAGCAAC GCCACCGTGG AGCACCTGCA CCCCATGGGC GATAACGATC 
     3721 TGGATGGCAG CTTCACCCGC ACCTTCAGCC TGCGCGACGG CGGCTACTAC AGCTCCGTGG 
     3781 TGGACAGCCA CATGCACTTC AAGAGCGCCA TCCACCCCAG CATCCTGCAG AACGGGGGCC 
     3841 CCATGTTCGC CTTCCGCCGC GTGGAGGAGG ATCACAGCAA CACCGAGCTG GGCATCGTGG 
     3901 AGTACCAGCA CGCCTTCAAG ACCCCGGATG CAGATGCCGG TGAAGAAAGA GTTTAAACGG 
     3961 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4021 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4081 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4141 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4201 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4261 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4321 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4381 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4441 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4501 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4561 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4621 GGTACGCGGC CAGCTTGGCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC 
     4681 TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA 
     4741 AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA 
     4801 ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT 
     4861 TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCGGCC 
     4921 TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG 
     4981 CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT 
     5041 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA 
     5101 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG 
     5161 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA 
     5221 CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT 
     5281 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG 
     5341 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA 
     5401 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT 
     5461 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC 
     5521 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC 
     5581 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA 
     5641 AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCCGA CCGCTATCAG 
     5701 GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC 
     5761 TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT 
     5821 CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGAAAT GACCGACCAA GCGACGCCCA 
     5881 ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA 
     5941 TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG CTGGAGTTCT 
     6001 TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA 
     6061 CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA 
     6121 TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG GCGTAATCAT 
     6181 GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG 
     6241 CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG 
     6301 CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA 
     6361 TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA 
     6421 CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG 
     6481 TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC 
     6541 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC 
     6601 CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC 
     6661 TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC 
     6721 TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA 
     6781 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC 
     6841 ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA 
     6901 ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG 
     6961 CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA 
     7021 GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG 
     7081 GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TTTTTTTGTT TGCAAGCAGC 
     7141 AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG 
     7201 ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA 
     7261 TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG 
     7321 AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT 
     7381 GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG 
     7441 AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC 
     7501 CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA 
     7561 CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC 
     7621 CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT 
     7681 CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC 
     7741 CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT 
     7801 TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC 
     7861 CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT 
     7921 GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA 
     7981 GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA 
     8041 TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG 
     8101 CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA 
     8161 AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT 
     8221 ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA 
     8281 AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT 
     8341 GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG 
     8401 CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG 
     8461 GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC 
     8521 GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT 
     8581 GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT 
     8641 TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT 
     8701 TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT 
     8761 TT
//