LOCUS       RG228533                8606 bp    DNA     circular  SYN 
DEFINITION  TBC1D15 (tGFP-tagged) - Human TBC1 domain family, member 15 (TBC1D15), transcript variant 2
ACCESSION   NM_001146214
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3087..3791 
                     /label="GFP"  
     misc_feature    3838..4423 
                     /label="PolyA signal"  
     misc_feature    6515..7160 
                     /label="ColE1"  
     misc_feature    4877..5671 
                     /label="Neo"  
     misc_feature    4508..4810 
                     /label="SV40 ori"  
     misc_feature    7175..8035 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3066..3072 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3066 
                     /label="TBC1D15"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTGTCCAGGA CTTTATCCAT ACTCCAGTTT GTTGGAGTAT GGTAGGAGTA 
     1081 TGATTATATA TGAACAAGAA GGAGTATATA TTCACTCATC TTGTGGAAAG ACCAATGACC 
     1141 AAGACGGCTT GATTTCAGGA ATATTACGTG TTTTAGAAAA GGATGCCGAA GTAATAGTGG 
     1201 ACTGGAGACC ATTGGATGAT GCATTAGATT CCTCTAGTAT TCTCTATGCT AGAAAGGACT 
     1261 CCAGTTCAGT TGTAGAATGG ACTCAGGCCC CAAAAGAAAG AGGTCATCGA GGATCAGAAC 
     1321 ATCTGAACAG TTACGAAGCA GAATGGGACA TGGTTAATAC AGTTTCATTT AAAAGGAAAC 
     1381 CACATACCAA TGGAGATGCT CCAAGTCATA GAAATGGGAA AAGCAAATGG TCATTCCTGT 
     1441 TCAGTTTGAC AGACCTGAAA TCAATCAAGC AAAACAAAGA GGGTATGGGC TGGTCCTATT 
     1501 TGGTATTCTG TCTAAAGGAT GACGTCGTTC TCCCTGCTCT ACACTTTCAT CAAGGAGATA 
     1561 GCAAACTACT GATTGAATCT CTTGAAAAAT ATGTGGTATT GTGTGAATCT CCACAGGATA 
     1621 AAAGAACACT TCTTGTGAAT TGTCAGAATA AGAGTCTTTC ACAGTCTTTT GAAAATCTTC 
     1681 TTGATGAGCC AGCATATGGT TTAATACAAA AAATTAAAAA GGACCCTTAT ACGGCAACTA 
     1741 TGATAGGATT TTCCAAAGTC ACAAACTACA TTTTTGACAG TTTGAGAGGC AGCGATCCCT 
     1801 CTACACATCA ACGACCACCT TCAGAAATGG CAGATTTTCT TAGTGATGCT ATTCCAGGTC 
     1861 TAAAGATAAA TCAACAAGAA GAACCAGGAT TTGAAGTCAT CACAAGAATT GATTTGGGGG 
     1921 AACGCCCTGT TGTTCAAAGG AGAGAACCGG TATCACTGGA AGAATGGACT AAGAACATTG 
     1981 ATTCTGAAGG AAGAATTTTA AATGTAGATA ATATGAAGCA GATGATATTT AGAGGGGGAC 
     2041 TTAGTCATGC ATTGAGAAAG CAAGCATGGA AATTTCTTCT GGGTTATTTT CCCTGGGACA 
     2101 GTACCAAGGA GGAAAGAACC CAATTACAAA AGCAAAAAAC TGATGAATAC TTCAGAATGA 
     2161 AACTGCAGTG GAAATCCATC AGCCAGGAAC AAGAGAAAAG AAATTCGAGG TTAAGAGATT 
     2221 ATAGAAGTCT TATCGAAAAA GATGTTAACA GAACAGATCG AACAAACAAG TTTTATGAAG 
     2281 GCCAAGATAA TCCAGGGTTG ATTTTACTTC ATGACATTTT GATGACCTAC TGTATGTATG 
     2341 ATTTTGATTT AGGATATGTT CAAGGAATGA GTGATTTACT TTCCCCTCTT TTATATGTGA 
     2401 TGGAAAATGA AGTGGATGCC TTTTGGTGCT TTGCCTCTTA CATGGACCAA ATGCATCAGA 
     2461 ATTTTGAAGA ACAAATGCAA GGCATGAAGA CCCAGCTAAT TCAGCTGAGT ACCTTACTTC 
     2521 GATTGTTAGA CAGTGGATTT TGCAGTTACT TAGAATCTCA GGACTCTGGA TACCTTTATT 
     2581 TTTGCTTCAG GTGGCTTTTA ATCAGATTCA AAAGGGAATT TAGTTTTCTA GATATTCTTC 
     2641 GATTATGGGA GGTAATGTGG ACCGAACTAC CATGTACAAA TTTCCATCTT CTTCTCTGTT 
     2701 GTGCTATTCT GGAATCAGAA AAGCAGCAAA TAATGGAAAA GCATTATGGC TTCAATGAAA 
     2761 TACTTAAGCA TATCAATGAA TTGTCCATGA AAATTGATGT GGAAGATATA CTCTGCAAGG 
     2821 CAGAAGCAAT TTCTCTACAG ATGGTAAAAT GCAAGGAATT GCCACAAGCA GTCTGTGAGA 
     2881 TCCTTGGGCT TCAAGGCAGT GAAGTTACAA CACCAGATTC AGACGTTGGT GAAGACGAAA 
     2941 ATGTTGTCAT GACTCCTTGT CCTACATCTG CATTTCAAAG TAATGCCTTG CCTACACTCT 
     3001 CTGCCAGTGG AGCCAGAAAT GACAGCCCAA CACAGATACC AGTGTCCTCA GATGTCTGCA 
     3061 GATTAACACC TGCAACGCGT ACGCGGCCGC TCGAGATGGA GAGCGACGAG AGCGGCCTGC 
     3121 CCGCCATGGA GATCGAGTGC CGCATCACCG GCACCCTGAA CGGCGTGGAG TTCGAGCTGG 
     3181 TGGGCGGCGG AGAGGGCACC CCCGAGCAGG GCCGCATGAC CAACAAGATG AAGAGCACCA 
     3241 AAGGCGCCCT GACCTTCAGC CCCTACCTGC TGAGCCACGT GATGGGCTAC GGCTTCTACC 
     3301 ACTTCGGCAC CTACCCCAGC GGCTACGAGA ACCCCTTCCT GCACGCCATC AACAACGGCG 
     3361 GCTACACCAA CACCCGCATC GAGAAGTACG AGGACGGCGG CGTGCTGCAC GTGAGCTTCA 
     3421 GCTACCGCTA CGAGGCCGGC CGCGTGATCG GCGACTTCAA GGTGATGGGC ACCGGCTTCC 
     3481 CCGAGGACAG CGTGATCTTC ACCGACAAGA TCATCCGCAG CAACGCCACC GTGGAGCACC 
     3541 TGCACCCCAT GGGCGATAAC GATCTGGATG GCAGCTTCAC CCGCACCTTC AGCCTGCGCG 
     3601 ACGGCGGCTA CTACAGCTCC GTGGTGGACA GCCACATGCA CTTCAAGAGC GCCATCCACC 
     3661 CCAGCATCCT GCAGAACGGG GGCCCCATGT TCGCCTTCCG CCGCGTGGAG GAGGATCACA 
     3721 GCAACACCGA GCTGGGCATC GTGGAGTACC AGCACGCCTT CAAGACCCCG GATGCAGATG 
     3781 CCGGTGAAGA AAGAGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     3841 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     3901 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     3961 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4021 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4081 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4141 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4201 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4261 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4321 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     4381 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     4441 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCTT GGCTGTGGAA TGTGTGTCAG 
     4501 TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC 
     4561 AATTAGTCAG CAACCAGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA 
     4621 AGCATGCATC TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC 
     4681 CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT 
     4741 GCAGAGGCCG AGGCCGCCTC GGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT 
     4801 GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT 
     4861 CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT 
     4921 CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC 
     4981 TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC 
     5041 GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC 
     5101 ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG 
     5161 CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG 
     5221 AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC 
     5281 CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT 
     5341 CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC 
     5401 GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC 
     5461 TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG 
     5521 CTGGGTGTGG CCGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG 
     5581 CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG 
     5641 CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG 
     5701 AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT 
     5761 TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC 
     5821 GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAATG 
     5881 GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT 
     5941 CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGTATA CCGTCGACCT 
     6001 CTAGCTAGAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC 
     6061 TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT 
     6121 GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC 
     6181 TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG 
     6241 GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG 
     6301 CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG 
     6361 GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC 
     6421 TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC 
     6481 AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC 
     6541 TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT 
     6601 CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG 
     6661 TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT 
     6721 CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG 
     6781 CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT 
     6841 GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC 
     6901 CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA 
     6961 GCGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC 
     7021 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT 
     7081 TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT 
     7141 TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA 
     7201 GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG 
     7261 TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC 
     7321 CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG 
     7381 CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC 
     7441 GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA 
     7501 CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC 
     7561 GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC 
     7621 CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC 
     7681 TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT 
     7741 CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA 
     7801 TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT 
     7861 CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA 
     7921 CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA 
     7981 AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC 
     8041 TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG 
     8101 GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC 
     8161 GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA 
     8221 CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC 
     8281 CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT 
     8341 TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG 
     8401 GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA 
     8461 CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT 
     8521 ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA 
     8581 TTTAACAAAA ATTTAACGCG AATTTT
//