LOCUS       RG228523                8546 bp    DNA     circular  SYN 
DEFINITION  DISC1 (tGFP-tagged) - Human disrupted in schizophrenia 1 (DISC1), transcript variant g
ACCESSION   NM_001164544
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3027..3731 
                     /label="GFP"  
     misc_feature    3778..4363 
                     /label="PolyA signal"  
     misc_feature    6455..7100 
                     /label="ColE1"  
     misc_feature    4817..5611 
                     /label="Neo"  
     misc_feature    4448..4750 
                     /label="SV40 ori"  
     misc_feature    7115..7975 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3006..3012 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3006 
                     /label="DISC1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCAGGCGGG GGTCCTCAGG GCGCCCCAGC CGCCGCCGGC GGCGGCGGCG 
     1081 TGAGCCACCG CGCAGGCAGC CGGGATTGCT TACCACCTGC AGCGTGCTTT CGGAGGCGGC 
     1141 GGCTGGCACG GAGGCCGGGC TACATGAGAA GCTCGACAGG GCCTGGGATC GGGTTCCTTT 
     1201 CCCCAGCAGT GGGCACACTG TTCCGGTTCC CAGGAGGGGT GTCTGGCGAG GAGTCCCACC 
     1261 ACTCGGAGTC CAGGGCCAGA CAGTGTGGCC TTGACTCGAG AGGCCTCTTG GTCCGGAGCC 
     1321 CTGTTTCCAA GAGTGCAGCA GCCCCTACTG TGACCTCTGT GAGAGGAACC TCGGCGCACT 
     1381 TTGGGATTCA GCTCAGAGGT GGCACCAGAT TGCCTGACAG GCTTAGCTGG CCGTGTGGCC 
     1441 CTGGGAGTGC TGGGTGGCAG CAAGAGTTTG CAGCCATGGA TAGTTCTGAG ACCCTGGACG 
     1501 CCAGCTGGGA GGCAGCCTGC AGCGATGGAG CAAGGCGTGT CCGGGCAGCA GGCTCTCTGC 
     1561 CATCAGCAGA GTTGAGTAGC AACAGCTGCA GCCCTGGCTG TGGCCCTGAG GTCCCCCCAA 
     1621 CCCCTCCTGG CTCTCACAGT GCCTTTACCT CAAGCTTTAG CTTTATTCGG CTCTCGCTTG 
     1681 GCTCTGCCGG GGAACGTGGA GAAGCAGAAG GCTGCCCACC ATCCAGAGAG GCTGAGTCCC 
     1741 ATTGCCAGAG CCCCCAGGAG ATGGGAGCCA AAGCTGCCAG CTTGGACGGG CCTCACGAGG 
     1801 ACCCGCGATG TCTCTCTCGG CCCTTCAGTC TCTTGGCTAC ACGGGTCTCT GCAGACTTGG 
     1861 CCCAGGCCGC AAGGAACAGC TCCAGGCCAG AGCGTGACAT GCATTCTTTA CCAGACATGG 
     1921 ACCCTGGCTC CTCCAGTTCT CTGGATCCCT CACTGGCTGG CTGTGGTGGT GATGGGAGCA 
     1981 GCGGCTCAGG GGATGCCCAC TCTTGGGACA CCCTGCTCAG GAAATGGGAG CCAGTGCTGC 
     2041 GGGACTGCCT GCTGAGAAAC CGGAGGCAGA TGGAGGTAAT ATCCTTAAGA TTAAAACTTC 
     2101 AGAAACTTCA GGAAGATGCA GTTGAGAATG ATGATTATGA TAAAGCTGAG ACGTTACAAC 
     2161 AAAGATTAGA AGACCTGGAA CAAGAGAAAA TCAGCCTGCA CTTTCAACTT CCTTCAAGGC 
     2221 AGCCAGCTCT TAGCAGTTTC CTGGGTCACC TGGCAGCACA AGTCCAGGCT GCCTTGCGCC 
     2281 GTGGGGCCAC TCAGCAGGCC AGCGGAGATG ACACCCACAC CCCACTGAGA ATGGAGCCGA 
     2341 GGCTGTTGGA ACCCACTGCT CAGGACAGCT TGCACGTGTC CATCACGAGA CGAGACTGGC 
     2401 TTCTTCAGGA AAAGCAGCAG CTACAGAAAG AAATCGAAGC TCTCCAAGCA AGGATGTTTG 
     2461 TGCTGGAAGC CAAAGATCAA CAGCTGAGAA GGGAAATAGA GGAGCAAGAG CAGCAACTCC 
     2521 AGTGGCAGGG CTGCGACCTG ACCCCACTGG TGGGCCAGCT GTCCCTGGGT CAGCTGCAGG 
     2581 AGGTCAGCAA GGCCTTGCAG GACACCCTGG CCTCAGCCGG TCAGATTCCC TTCCATGCAG 
     2641 AGCCACCGGA AACCATAAGG AGCCTCCAGG AAAGAATAAA ATCCCTCAAC TTGTCACTTA 
     2701 AAGAAATCAC TACTAAGGTG TGTATGAGTG AGAAATTCTG CAGCACCCTG AGGAAGAAAG 
     2761 TTAACGATAT TGAAACCCAA CTACCAGCCT TGCTTGAAGC CAAAATGCAT GCCATATCAG 
     2821 GAAACCATTT CTGGACGGCT AAAGACCTCA CCGAGGAGAT TAGATCATTA ACATCAGAGA 
     2881 GAGAAGGGCT GGAGGGACTC CTCAGCAAGC TGTTGGTGTT GAGTTCCAGG AATGTCAAAA 
     2941 AGCTGGGAAG TGTTAAAGAA GATTACAACA GACTGAGAAG AGAAGTGGAG CACCAGGAGA 
     3001 CTGCCTATGG TAGGACGCGT ACGCGGCCGC TCGAGATGGA GAGCGACGAG AGCGGCCTGC 
     3061 CCGCCATGGA GATCGAGTGC CGCATCACCG GCACCCTGAA CGGCGTGGAG TTCGAGCTGG 
     3121 TGGGCGGCGG AGAGGGCACC CCCGAGCAGG GCCGCATGAC CAACAAGATG AAGAGCACCA 
     3181 AAGGCGCCCT GACCTTCAGC CCCTACCTGC TGAGCCACGT GATGGGCTAC GGCTTCTACC 
     3241 ACTTCGGCAC CTACCCCAGC GGCTACGAGA ACCCCTTCCT GCACGCCATC AACAACGGCG 
     3301 GCTACACCAA CACCCGCATC GAGAAGTACG AGGACGGCGG CGTGCTGCAC GTGAGCTTCA 
     3361 GCTACCGCTA CGAGGCCGGC CGCGTGATCG GCGACTTCAA GGTGATGGGC ACCGGCTTCC 
     3421 CCGAGGACAG CGTGATCTTC ACCGACAAGA TCATCCGCAG CAACGCCACC GTGGAGCACC 
     3481 TGCACCCCAT GGGCGATAAC GATCTGGATG GCAGCTTCAC CCGCACCTTC AGCCTGCGCG 
     3541 ACGGCGGCTA CTACAGCTCC GTGGTGGACA GCCACATGCA CTTCAAGAGC GCCATCCACC 
     3601 CCAGCATCCT GCAGAACGGG GGCCCCATGT TCGCCTTCCG CCGCGTGGAG GAGGATCACA 
     3661 GCAACACCGA GCTGGGCATC GTGGAGTACC AGCACGCCTT CAAGACCCCG GATGCAGATG 
     3721 CCGGTGAAGA AAGAGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     3781 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     3841 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     3901 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     3961 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4021 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4081 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4141 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4201 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4261 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     4321 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     4381 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCTT GGCTGTGGAA TGTGTGTCAG 
     4441 TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC 
     4501 AATTAGTCAG CAACCAGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA 
     4561 AGCATGCATC TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC 
     4621 CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT 
     4681 GCAGAGGCCG AGGCCGCCTC GGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT 
     4741 GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT 
     4801 CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT 
     4861 CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC 
     4921 TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC 
     4981 GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC 
     5041 ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG 
     5101 CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG 
     5161 AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC 
     5221 CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT 
     5281 CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC 
     5341 GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC 
     5401 TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG 
     5461 CTGGGTGTGG CCGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG 
     5521 CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG 
     5581 CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG 
     5641 AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT 
     5701 TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC 
     5761 GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAATG 
     5821 GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT 
     5881 CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGTATA CCGTCGACCT 
     5941 CTAGCTAGAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC 
     6001 TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT 
     6061 GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC 
     6121 TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG 
     6181 GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG 
     6241 CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG 
     6301 GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC 
     6361 TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC 
     6421 AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC 
     6481 TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT 
     6541 CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG 
     6601 TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT 
     6661 CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG 
     6721 CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT 
     6781 GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC 
     6841 CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA 
     6901 GCGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC 
     6961 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT 
     7021 TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT 
     7081 TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA 
     7141 GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG 
     7201 TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC 
     7261 CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG 
     7321 CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC 
     7381 GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA 
     7441 CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC 
     7501 GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC 
     7561 CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC 
     7621 TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT 
     7681 CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA 
     7741 TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT 
     7801 CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA 
     7861 CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA 
     7921 AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC 
     7981 TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG 
     8041 GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC 
     8101 GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA 
     8161 CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC 
     8221 CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT 
     8281 TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG 
     8341 GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA 
     8401 CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT 
     8461 ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA 
     8521 TTTAACAAAA ATTTAACGCG AATTTT
//