LOCUS       RG227896                9086 bp    DNA     circular  SYN 
DEFINITION  PROM1 (tGFP-tagged) - Human prominin 1 (PROM1), transcript variant 7
ACCESSION   NM_001145849
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3567..4271 
                     /label="GFP"  
     misc_feature    4318..4903 
                     /label="PolyA signal"  
     misc_feature    6995..7640 
                     /label="ColE1"  
     misc_feature    5357..6151 
                     /label="Neo"  
     misc_feature    4988..5290 
                     /label="SV40 ori"  
     misc_feature    7655..8515 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3546..3552 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3546 
                     /label="PROM1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCTCGTA CTCGGCTCCC TGTTGCTGCT GGGGCTGTGC GGGAACTCCT 
     1081 TTTCAGGAGG GCAGCCTTCA TCCACAGATG CTCCTAAGGC TTGGAATTAT GAATTGCCTG 
     1141 CAACAAATTA TGAGACCCAA GACTCCCATA AAGCTGGACC CATTGGCATT CTCTTTGAAC 
     1201 TAGTGCATAT CTTTCTCTAT GTGGTACAGC CGCGTGATTT CCCAGAAGAT ACTTTGAGAA 
     1261 AATTCTTACA GAAGGCATAT GAATCCAAAA TTGATTATGA CAAGCCAGAA ACTGTAATCT 
     1321 TAGGTCTAAA GATTGTCTAC TATGAAGCAG GGATTATTCT ATGCTGTGTC CTGGGGCTGC 
     1381 TGTTTATTAT TCTGATGCCT CTGGTGGGGT ATTTCTTTTG TATGTGTCGT TGCTGTAACA 
     1441 AATGTGGTGG AGAAATGCAC CAGCGACAGA AGGAAAATGG GCCCTTCCTG AGGAAATGCT 
     1501 TTGCAATCTC CCTGTTGGTG ATTTGTATAA TAATAAGCAT TGGCATCTTC TATGGTTTTG 
     1561 TGGCAAATCA CCAGGTAAGA ACCCGGATCA AAAGGAGTCG GAAACTGGCA GATAGCAATT 
     1621 TCAAGGACTT GCGAACTCTC TTGAATGAAA CTCCAGAGCA AATCAAATAT ATATTGGCCC 
     1681 AGTACAACAC TACCAAGGAC AAGGCGTTCA CAGATCTGAA CAGTATCAAT TCAGTGCTAG 
     1741 GAGGCGGAAT TCTTGACCGA CTGAGACCCA ACATCATCCC TGTTCTTGAT GAGATTAAGT 
     1801 CCATGGCAAC AGCGATCAAG GAGACCAAAG AGGCGTTGGA GAACATGAAC AGCACCTTGA 
     1861 AGAGCTTGCA CCAACAAAGT ACACAGCTTA GCAGCAGTCT GACCAGCGTG AAAACTAGCC 
     1921 TGCGGTCATC TCTCAATGAC CCTCTGTGCT TGGTGCATCC ATCAAGTGAA ACCTGCAACA 
     1981 GCATCAGATT GTCTCTAAGC CAGCTGAATA GCAACCCTGA ACTGAGGCAG CTTCCACCCG 
     2041 TGGATGCAGA ACTTGACAAC GTTAATAACG TTCTTAGGAC AGATTTGGAT GGCCTGGTCC 
     2101 AACAGGGCTA TCAATCCCTT AATGATATAC CTGACAGAGT ACAACGCCAA ACCACGACTG 
     2161 TCGTAGCAGG TATCAAAAGG GTCTTGAATT CCATTGGTTC AGATATCGAC AATGTAACTC 
     2221 AGCGTCTTCC TATTCAGGAT ATACTCTCAG CATTCTCTGT TTATGTTAAT AACACTGAAA 
     2281 GTTACATCCA CAGAAATTTA CCTACATTGG AAGAGTATGA TTCATACTGG TGGCTGGGTG 
     2341 GCCTGGTCAT CTGCTCTCTG CTGACCCTCA TCGTGATTTT TTACTACCTG GGCTTACTGT 
     2401 GTGGCGTGTG CGGCTATGAC AGGCATGCCA CCCCGACCAC CCGAGGCTGT GTCTCCAACA 
     2461 CCGGAGGCGT CTTCCTCATG GTTGGAGTTG GATTAAGTTT CCTCTTTTGC TGGATATTGA 
     2521 TGATCATTGT GGTTCTTACC TTTGTCTTTG GTGCAAATGT GGAAAAACTG ATCTGTGAAC 
     2581 CTTACACGAG CAAGGAATTA TTCCGGGTTT TGGATACACC CTACTTACTA AATGAAGACT 
     2641 GGGAATACTA TCTCTCTGGG AAGCTATTTA ATAAATCAAA AATGAAGCTC ACTTTTGAAC 
     2701 AAGTTTACAG TGACTGCAAA AAAAATAGAG GCACTTACGG CACTCTTCAC CTGCAGAACA 
     2761 GCTTCAATAT CAGTGAACAT CTCAACATTA ATGAGCATAC TGGAAGCATA AGCAGTGAAT 
     2821 TGGAAAGTCT GAAGGTAAAT CTTAATATCT TTCTGTTGGG TGCAGCAGGA AGAAAAAACC 
     2881 TTCAGGATTT TGCTGCTTGT GGAATAGACA GAATGAATTA TGACAGCTAC TTGGCTCAGA 
     2941 CTGGTAAATC CCCCGCAGGA GTGAATCTTT TATCATTTGC ATATGATCTA GAAGCAAAAG 
     3001 CAAACAGTTT GCCCCCAGGA AATTTGAGGA ACTCCCTGAA AAGAGATGCA CAAACTATTA 
     3061 AAACAATTCA CCAGCAACGA GTCCTTCCTA TAGAACAATC ACTGAGCACT CTATACCAAA 
     3121 GCGTCAAGAT ACTTCAACGC ACAGGGAATG GATTGTTGGA GAGAGTAACT AGGATTCTAG 
     3181 CTTCTCTGGA TTTTGCTCAG AACTTCATCA CAAACAATAC TTCCTCTGTT ATTATTGAGG 
     3241 AAACTAAGAA GTATGGGAGA ACAATAATAG GATATTTTGA ACATTATCTG CAGTGGATCG 
     3301 AGTTCTCTAT CAGTGAGAAA GTGGCATCGT GCAAACCTGT GGCCACCGCT CTAGATACTG 
     3361 CTGTTGATGT CTTTCTGTGT AGCTACATTA TCGACCCCTT GAATTTGTTT TGGTTTGGCA 
     3421 TAGGAAAAGC TACTGTATTT TTACTTCCGG CTCTAATTTT TGCGGTAAAA CTGGCTAAGT 
     3481 ACTATCGTCG AATGGATTCG GAGGACGTGT ACGATGATGT TGAAACTATA CCCATGAAAA 
     3541 ACCCATCACA ACATACGCGT ACGCGGCCGC TCGAGATGGA GAGCGACGAG AGCGGCCTGC 
     3601 CCGCCATGGA GATCGAGTGC CGCATCACCG GCACCCTGAA CGGCGTGGAG TTCGAGCTGG 
     3661 TGGGCGGCGG AGAGGGCACC CCCGAGCAGG GCCGCATGAC CAACAAGATG AAGAGCACCA 
     3721 AAGGCGCCCT GACCTTCAGC CCCTACCTGC TGAGCCACGT GATGGGCTAC GGCTTCTACC 
     3781 ACTTCGGCAC CTACCCCAGC GGCTACGAGA ACCCCTTCCT GCACGCCATC AACAACGGCG 
     3841 GCTACACCAA CACCCGCATC GAGAAGTACG AGGACGGCGG CGTGCTGCAC GTGAGCTTCA 
     3901 GCTACCGCTA CGAGGCCGGC CGCGTGATCG GCGACTTCAA GGTGATGGGC ACCGGCTTCC 
     3961 CCGAGGACAG CGTGATCTTC ACCGACAAGA TCATCCGCAG CAACGCCACC GTGGAGCACC 
     4021 TGCACCCCAT GGGCGATAAC GATCTGGATG GCAGCTTCAC CCGCACCTTC AGCCTGCGCG 
     4081 ACGGCGGCTA CTACAGCTCC GTGGTGGACA GCCACATGCA CTTCAAGAGC GCCATCCACC 
     4141 CCAGCATCCT GCAGAACGGG GGCCCCATGT TCGCCTTCCG CCGCGTGGAG GAGGATCACA 
     4201 GCAACACCGA GCTGGGCATC GTGGAGTACC AGCACGCCTT CAAGACCCCG GATGCAGATG 
     4261 CCGGTGAAGA AAGAGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4321 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4381 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4441 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4501 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4561 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4621 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4681 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4741 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4801 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     4861 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     4921 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCTT GGCTGTGGAA TGTGTGTCAG 
     4981 TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC 
     5041 AATTAGTCAG CAACCAGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA 
     5101 AGCATGCATC TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC 
     5161 CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT 
     5221 GCAGAGGCCG AGGCCGCCTC GGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT 
     5281 GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT 
     5341 CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT 
     5401 CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC 
     5461 TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC 
     5521 GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC 
     5581 ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG 
     5641 CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG 
     5701 AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC 
     5761 CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT 
     5821 CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC 
     5881 GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC 
     5941 TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG 
     6001 CTGGGTGTGG CCGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG 
     6061 CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG 
     6121 CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG 
     6181 AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT 
     6241 TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC 
     6301 GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAATG 
     6361 GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT 
     6421 CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGTATA CCGTCGACCT 
     6481 CTAGCTAGAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC 
     6541 TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT 
     6601 GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC 
     6661 TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG 
     6721 GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG 
     6781 CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG 
     6841 GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC 
     6901 TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC 
     6961 AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC 
     7021 TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT 
     7081 CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG 
     7141 TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT 
     7201 CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG 
     7261 CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT 
     7321 GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC 
     7381 CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA 
     7441 GCGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC 
     7501 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT 
     7561 TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT 
     7621 TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA 
     7681 GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG 
     7741 TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC 
     7801 CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG 
     7861 CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC 
     7921 GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA 
     7981 CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC 
     8041 GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC 
     8101 CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC 
     8161 TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT 
     8221 CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA 
     8281 TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT 
     8341 CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA 
     8401 CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA 
     8461 AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC 
     8521 TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG 
     8581 GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC 
     8641 GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA 
     8701 CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC 
     8761 CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT 
     8821 TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG 
     8881 GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA 
     8941 CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT 
     9001 ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA 
     9061 TTTAACAAAA ATTTAACGCG AATTTT
//