LOCUS       RG227874                9128 bp    DNA     circular  SYN 
DEFINITION  PROM1 (tGFP-tagged) - Human prominin 1 (PROM1), transcript variant 3
ACCESSION   NM_001145848
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3609..4313 
                     /label="GFP"  
     misc_feature    4360..4945 
                     /label="PolyA signal"  
     misc_feature    7037..7682 
                     /label="ColE1"  
     misc_feature    5399..6193 
                     /label="Neo"  
     misc_feature    5030..5332 
                     /label="SV40 ori"  
     misc_feature    7697..8557 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3588..3594 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3588 
                     /label="PROM1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCTCGTA CTCGGCTCCC TGTTGCTGCT GGGGCTGTGC GGGAACTCCT 
     1081 TTTCAGGAGG GCAGCCTTCA TCCACAGATG CTCCTAAGGC TTGGAATTAT GAATTGCCTG 
     1141 CAACAAATTA TGAGACCCAA GACTCCCATA AAGCTGGACC CATTGGCATT CTCTTTGAAC 
     1201 TAGTGCATAT CTTTCTCTAT GTGGTACAGC CGCGTGATTT CCCAGAAGAT ACTTTGAGAA 
     1261 AATTCTTACA GAAGGCATAT GAATCCAAAA TTGATTATGA CAAGATTGTC TACTATGAAG 
     1321 CAGGGATTAT TCTATGCTGT GTCCTGGGGC TGCTGTTTAT TATTCTGATG CCTCTGGTGG 
     1381 GGTATTTCTT TTGTATGTGT CGTTGCTGTA ACAAATGTGG TGGAGAAATG CACCAGCGAC 
     1441 AGAAGGAAAA TGGGCCCTTC CTGAGGAAAT GCTTTGCAAT CTCCCTGTTG GTGATTTGTA 
     1501 TAATAATAAG CATTGGCATC TTCTATGGTT TTGTGGCAAA TCACCAGGTA AGAACCCGGA 
     1561 TCAAAAGGAG TCGGAAACTG GCAGATAGCA ATTTCAAGGA CTTGCGAACT CTCTTGAATG 
     1621 AAACTCCAGA GCAAATCAAA TATATATTGG CCCAGTACAA CACTACCAAG GACAAGGCGT 
     1681 TCACAGATCT GAACAGTATC AATTCAGTGC TAGGAGGCGG AATTCTTGAC CGACTGAGAC 
     1741 CCAACATCAT CCCTGTTCTT GATGAGATTA AGTCCATGGC AACAGCGATC AAGGAGACCA 
     1801 AAGAGGCGTT GGAGAACATG AACAGCACCT TGAAGAGCTT GCACCAACAA AGTACACAGC 
     1861 TTAGCAGCAG TCTGACCAGC GTGAAAACTA GCCTGCGGTC ATCTCTCAAT GACCCTCTGT 
     1921 GCTTGGTGCA TCCATCAAGT GAAACCTGCA ACAGCATCAG ATTGTCTCTA AGCCAGCTGA 
     1981 ATAGCAACCC TGAACTGAGG CAGCTTCCAC CCGTGGATGC AGAACTTGAC AACGTTAATA 
     2041 ACGTTCTTAG GACAGATTTG GATGGCCTGG TCCAACAGGG CTATCAATCC CTTAATGATA 
     2101 TACCTGACAG AGTACAACGC CAAACCACGA CTGTCGTAGC AGGTATCAAA AGGGTCTTGA 
     2161 ATTCCATTGG TTCAGATATC GACAATGTAA CTCAGCGTCT TCCTATTCAG GATATACTCT 
     2221 CAGCATTCTC TGTTTATGTT AATAACACTG AAAGTTACAT CCACAGAAAT TTACCTACAT 
     2281 TGGAAGAGTA TGATTCATAC TGGTGGCTGG GTGGCCTGGT CATCTGCTCT CTGCTGACCC 
     2341 TCATCGTGAT TTTTTACTAC CTGGGCTTAC TGTGTGGCGT GTGCGGCTAT GACAGGCATG 
     2401 CCACCCCGAC CACCCGAGGC TGTGTCTCCA ACACCGGAGG CGTCTTCCTC ATGGTTGGAG 
     2461 TTGGATTAAG TTTCCTCTTT TGCTGGATAT TGATGATCAT TGTGGTTCTT ACCTTTGTCT 
     2521 TTGGTGCAAA TGTGGAAAAA CTGATCTGTG AACCTTACAC GAGCAAGGAA TTATTCCGGG 
     2581 TTTTGGATAC ACCCTACTTA CTAAATGAAG ACTGGGAATA CTATCTCTCT GGGAAGCTAT 
     2641 TTAATAAATC AAAAATGAAG CTCACTTTTG AACAAGTTTA CAGTGACTGC AAAAAAAATA 
     2701 GAGGCACTTA CGGCACTCTT CACCTGCAGA ACAGCTTCAA TATCAGTGAA CATCTCAACA 
     2761 TTAATGAGCA TACTGGAAGC ATAAGCAGTG AATTGGAAAG TCTGAAGGTA AATCTTAATA 
     2821 TCTTTCTGTT GGGTGCAGCA GGAAGAAAAA ACCTTCAGGA TTTTGCTGCT TGTGGAATAG 
     2881 ACAGAATGAA TTATGACAGC TACTTGGCTC AGACTGGTAA ATCCCCCGCA GGAGTGAATC 
     2941 TTTTATCATT TGCATATGAT CTAGAAGCAA AAGCAAACAG TTTGCCCCCA GGAAATTTGA 
     3001 GGAACTCCCT GAAAAGAGAT GCACAAACTA TTAAAACAAT TCACCAGCAA CGAGTCCTTC 
     3061 CTATAGAACA ATCACTGAGC ACTCTATACC AAAGCGTCAA GATACTTCAA CGCACAGGGA 
     3121 ATGGATTGTT GGAGAGAGTA ACTAGGATTC TAGCTTCTCT GGATTTTGCT CAGAACTTCA 
     3181 TCACAAACAA TACTTCCTCT GTTATTATTG AGGAAACTAA GAAGTATGGG AGAACAATAA 
     3241 TAGGATATTT TGAACATTAT CTGCAGTGGA TCGAGTTCTC TATCAGTGAG AAAGTGGCAT 
     3301 CGTGCAAACC TGTGGCCACC GCTCTAGATA CTGCTGTTGA TGTCTTTCTG TGTAGCTACA 
     3361 TTATCGACCC CTTGAATTTG TTTTGGTTTG GCATAGGAAA AGCTACTGTA TTTTTACTTC 
     3421 CGGCTCTAAT TTTTGCGGTA AAACTGGCTA AGTACTATCG TCGAATGGAT TCGGAGGACG 
     3481 TGTACGATGA TGTTGAAACT ATACCCATGA AAAATATGGA AAATGGTAAT AATGGTTATC 
     3541 ATAAAGATCA TGTATATGGT ATTCACAATC CTGTTATGAC AAGCCCATCA CAACATACGC 
     3601 GTACGCGGCC GCTCGAGATG GAGAGCGACG AGAGCGGCCT GCCCGCCATG GAGATCGAGT 
     3661 GCCGCATCAC CGGCACCCTG AACGGCGTGG AGTTCGAGCT GGTGGGCGGC GGAGAGGGCA 
     3721 CCCCCGAGCA GGGCCGCATG ACCAACAAGA TGAAGAGCAC CAAAGGCGCC CTGACCTTCA 
     3781 GCCCCTACCT GCTGAGCCAC GTGATGGGCT ACGGCTTCTA CCACTTCGGC ACCTACCCCA 
     3841 GCGGCTACGA GAACCCCTTC CTGCACGCCA TCAACAACGG CGGCTACACC AACACCCGCA 
     3901 TCGAGAAGTA CGAGGACGGC GGCGTGCTGC ACGTGAGCTT CAGCTACCGC TACGAGGCCG 
     3961 GCCGCGTGAT CGGCGACTTC AAGGTGATGG GCACCGGCTT CCCCGAGGAC AGCGTGATCT 
     4021 TCACCGACAA GATCATCCGC AGCAACGCCA CCGTGGAGCA CCTGCACCCC ATGGGCGATA 
     4081 ACGATCTGGA TGGCAGCTTC ACCCGCACCT TCAGCCTGCG CGACGGCGGC TACTACAGCT 
     4141 CCGTGGTGGA CAGCCACATG CACTTCAAGA GCGCCATCCA CCCCAGCATC CTGCAGAACG 
     4201 GGGGCCCCAT GTTCGCCTTC CGCCGCGTGG AGGAGGATCA CAGCAACACC GAGCTGGGCA 
     4261 TCGTGGAGTA CCAGCACGCC TTCAAGACCC CGGATGCAGA TGCCGGTGAA GAAAGAGTTT 
     4321 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4381 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4441 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4501 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4561 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4621 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4681 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4741 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     4801 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     4861 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     4921 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     4981 GAATTGGGTA CGCGGCCAGC TTGGCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC 
     5041 CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG 
     5101 TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG 
     5161 TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC 
     5221 GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC 
     5281 TCGGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC 
     5341 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAAGAGAC AGGATGAGGA 
     5401 TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG 
     5461 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC 
     5521 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG 
     5581 AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC 
     5641 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG 
     5701 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT 
     5761 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG 
     5821 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT 
     5881 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC 
     5941 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG 
     6001 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCCGACCGC 
     6061 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT 
     6121 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT 
     6181 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA 
     6241 CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT 
     6301 TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG 
     6361 AGTTCTTCGC CCACCCCAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA 
     6421 GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA 
     6481 AACTCATCAA TGTATCTTAT CATGTCTGTA TACCGTCGAC CTCTAGCTAG AGCTTGGCGT 
     6541 AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA 
     6601 TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT 
     6661 TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT 
     6721 AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT 
     6781 CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA 
     6841 AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA 
     6901 AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC 
     6961 TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA 
     7021 CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC 
     7081 CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT 
     7141 CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT 
     7201 GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG 
     7261 AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA 
     7321 GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT 
     7381 ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA 
     7441 GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTTTT TTTGTTTGCA 
     7501 AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG 
     7561 GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA 
     7621 AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA 
     7681 TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG 
     7741 CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA 
     7801 TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC 
     7861 CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC 
     7921 CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA 
     7981 GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC 
     8041 GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT 
     8101 GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA 
     8161 GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG 
     8221 TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG 
     8281 AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC 
     8341 CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT 
     8401 CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT 
     8461 CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG 
     8521 CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC 
     8581 AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA 
     8641 TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG 
     8701 CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA 
     8761 CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT 
     8821 TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG 
     8881 CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT 
     8941 CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC 
     9001 TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG 
     9061 GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG 
     9121 CGAATTTT
//