LOCUS       RG226648                9164 bp    DNA     circular  SYN 
DEFINITION  PTPN3 (tGFP-tagged) - Human protein tyrosine phosphatase, non-receptor type 3 (PTPN3), transcript variant 2
ACCESSION   NM_001145368
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3645..4349 
                     /label="GFP"  
     misc_feature    4396..4981 
                     /label="PolyA signal"  
     misc_feature    7073..7718 
                     /label="ColE1"  
     misc_feature    5435..6229 
                     /label="Neo"  
     misc_feature    5066..5368 
                     /label="SV40 ori"  
     misc_feature    7733..8593 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3624..3630 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3624 
                     /label="PTPN3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACCTCCCGG TTACGTGCGT TGGGTGGAAG AATTAATAAT ATACGCACCT 
     1081 CGGAGTTACC CAAAGAGAAA ACTCGATCAG AAGTCATTTG CAGCATCCAC TTTTTAGATG 
     1141 GCGTGGTACA GACCTTTAAA GTTACTAAAC AAGACACTGG CCAGGTTCTT CTGGATATGG 
     1201 TGCACAACCA CCTGGGTGTG ACTGAAAAGG AATATTTTGG TTTACAGCAT GATGACGACT 
     1261 CCGTGGACTC TCCTAGATGG CTGGAAGCAA GCAAAGCCAT CAGGAAGCAG TTAAAAGGAG 
     1321 GTTTCCCCTG TACCCTGCAT TTTCGAGTAA GATTTTTTAT ACCTGATCCC AACACACTGC 
     1381 AGCAAGAACA AACCAGGCAC TTGTATTTCT TACAACTGAA GATGGATATT TGCGAAGGAA 
     1441 GGTTAACCTG CCCTCTTAAC TCAGCAGTGG TTCTAGCGTC CTATGCCGTA CAATCTCATT 
     1501 TTGGAGACTA TAATTCTTCC ATACATCATC CAGGCTATCT TTCCGATAGT CACTTTATAC 
     1561 CCGATCAAAA TGAGGACTTT TTAACAAAAG TCGAATCTCT GCATGAGCAG CACAGTGGGC 
     1621 TAAAACAATC AGAAGCAGAA TCCTGCTATA TCAACATAGC GCGGACCCTC GACTTCTATG 
     1681 GAGTAGAACT GCACAGTGGT AGGGATCTGC ACAATTTAGA CCTAATGATT GGAATTGCTT 
     1741 CCGCGGGTGT TGCTGTGTAC CGAAAATACA TTTGCACAAG TTTCTATCCT TGGGTGAACA 
     1801 TTCTCAAAAT TTCTTTCAAA AGGAAAAAGT TCTTCATACA TCAGCGACAG AAACAGGCTG 
     1861 AATCCAGGGA ACATATTGTG GCCTTCAACA TGCTGAATTA CCGATCTTGC AAAAACTTGT 
     1921 GGAAATCCTG TGTTGAGCAC CATACGTTCT TTCAGGCAAA GAAGCTACTA CCTCAGGAAA 
     1981 AGAATGTTCT GTCTCAGTAC TGGACTATGG GCTCTCGGAA CACCAAAAAG CGAAGTCCTC 
     2041 GGCTCCGGCA CGAAATCCGA AAGCCACGCC ACTCTTCTGC AGATAACCTT GCAAATGAAA 
     2101 TGACCTACAT CACGGAAACG GAAGATGTAT TTTACACGTA CAAGGGCTCT CTGGCCCCTC 
     2161 AAGACAGCGA TTCTGAAGTT TCTCAGAACC GAAGCCCGCA CCAAGAGAGT TTATCCGAGA 
     2221 ACAATCCGGC ACAAAGCTAC CTGACCCAGA AGTCATCCAG TTCTGTGTCT CCATCTTCAA 
     2281 ATGCTCCAGG CTCCTGCTCA CCTGACGGCG TTGATCAGCA GCTCTTAGAT GACTTCCACA 
     2341 GGGTGACCAA AGGGGGCTCC ACCGAGGACG CCAGCCAGTA CTACTGTGAC AAGAATGATA 
     2401 ATGGTGACAG CTACTTAGTC TTGATCCGTA TCACACCAGA TGAAGATGGA AAATTTGGAT 
     2461 TTAATCTTAA GGGAGGAGTG GATCAAAAGA TGCCTCTTGT GGTATCAAGG ATAAACCCAG 
     2521 AGTCACCTGC GGACACCTGC ATTCCTAAGC TGAACGAAGG GGATCAAATC GTGTTAATCA 
     2581 ATGGCCGGGA CATCTCAGAA CACACGCATG ACCAAGTGGT GATGTTCATC AAAGCCAGCC 
     2641 GGGAGTCCCA CTCACGGGAG CTGGCCCTGG TGATCAGGAG GAGAGCTGTC CGCTCATTTG 
     2701 CTGACTTCAA GTCTGAAGAT GAACTGAACC AGCTTTTCCC CGAAGCCATT TTCCCCATGT 
     2761 GTCCGGAGGG TGGGGACACT TTGGAGGGAT CCATGGCACA GCTAAAGAAG GGCCTCGAAA 
     2821 GCGGGACGGT GCTGATCCAG TTTGAGCAAC TCTACAGAAA AAAGCCAGGT TTGGCCATCA 
     2881 CGTTTGCAAA GCTGCCTCAA AATTTGGACA AAAACCGATA TAAAGATGTG CTGCCTTATG 
     2941 ACACCACCCG GGTATTATTG CAGGGAAATG AAGATTATAT TAATGCAAGT TACGTGAACA 
     3001 TGGAAATTCC TGCTGCTAAC CTTGTGAACA AGTACATCGC CACTCAGGGG CCCCTGCCGC 
     3061 ATACCTGTGC ACAGTTTTGG CAGGTTGTCT GGGATCAGAA GTTGTCACTC ATTGTCATGT 
     3121 TGACGACTCT CACAGAACGA GGGCGGACCA AATGTCACCA GTACTGGCCA GATCCCCCCG 
     3181 ACGTCATGAA CCACGGCGGC TTTCACATCC AGTGTCAGTC AGAGGACTGC ACCATCGCCT 
     3241 ATGTGTCCCG AGAAATGCTG GTCACAAACA CCCAGACCGG GGAAGAACAC ACAGTGACAC 
     3301 ATCTCCAGTA CGTCGCATGG CCTGACCACG GTGTGCCCGA TGACTCCTCC GACTTTCTGG 
     3361 AATTTGTAAA CTATGTGAGG TCTCTGAGAG TGGACAGCGA GCCCGTCCTA GTTCACTGCA 
     3421 GTGCTGGAAT AGGTCGAACC GGTGTGTTGG TCACTATGGA AACAGCCATG TGCCTAACTG 
     3481 AGAGGAACCT GCCCATTTAC CCACTGGATA TTGTCCGAAA AATGCGAGAC CAGCGCGCCA 
     3541 TGATGGTGCA GACATCAAGC CAGTACAAGT TTGTGTGTGA AGCGATTCTT CGTGTGTATG 
     3601 AAGAAGGTTT AGTCCAAATG CTGGATCCTA GTACGCGTAC GCGGCCGCTC GAGATGGAGA 
     3661 GCGACGAGAG CGGCCTGCCC GCCATGGAGA TCGAGTGCCG CATCACCGGC ACCCTGAACG 
     3721 GCGTGGAGTT CGAGCTGGTG GGCGGCGGAG AGGGCACCCC CGAGCAGGGC CGCATGACCA 
     3781 ACAAGATGAA GAGCACCAAA GGCGCCCTGA CCTTCAGCCC CTACCTGCTG AGCCACGTGA 
     3841 TGGGCTACGG CTTCTACCAC TTCGGCACCT ACCCCAGCGG CTACGAGAAC CCCTTCCTGC 
     3901 ACGCCATCAA CAACGGCGGC TACACCAACA CCCGCATCGA GAAGTACGAG GACGGCGGCG 
     3961 TGCTGCACGT GAGCTTCAGC TACCGCTACG AGGCCGGCCG CGTGATCGGC GACTTCAAGG 
     4021 TGATGGGCAC CGGCTTCCCC GAGGACAGCG TGATCTTCAC CGACAAGATC ATCCGCAGCA 
     4081 ACGCCACCGT GGAGCACCTG CACCCCATGG GCGATAACGA TCTGGATGGC AGCTTCACCC 
     4141 GCACCTTCAG CCTGCGCGAC GGCGGCTACT ACAGCTCCGT GGTGGACAGC CACATGCACT 
     4201 TCAAGAGCGC CATCCACCCC AGCATCCTGC AGAACGGGGG CCCCATGTTC GCCTTCCGCC 
     4261 GCGTGGAGGA GGATCACAGC AACACCGAGC TGGGCATCGT GGAGTACCAG CACGCCTTCA 
     4321 AGACCCCGGA TGCAGATGCC GGTGAAGAAA GAGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4381 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4441 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4501 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4561 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4621 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4681 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4741 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4801 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4861 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     4921 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     4981 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCTTGG 
     5041 CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 
     5101 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA 
     5161 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA 
     5221 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA 
     5281 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG 
     5341 TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA 
     5401 TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT 
     5461 GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA 
     5521 CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG 
     5581 GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG 
     5641 CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT 
     5701 GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT 
     5761 CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG 
     5821 CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT 
     5881 ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC 
     5941 GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC 
     6001 GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA 
     6061 TTCATCGACT GTGGCCGGCT GGGTGTGGCC GACCGCTATC AGGACATAGC GTTGGCTACC 
     6121 CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT 
     6181 ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA 
     6241 GCGGGACTCT GGGGTTCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT 
     6301 TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG 
     6361 GCTGGATGAT CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT 
     6421 TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG 
     6481 CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG 
     6541 TCTGTATACC GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG 
     6601 TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA 
     6661 AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG 
     6721 CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA 
     6781 GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG 
     6841 TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG 
     6901 AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC 
     6961 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA 
     7021 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT 
     7081 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC 
     7141 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC 
     7201 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC 
     7261 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT 
     7321 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG 
     7381 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA 
     7441 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA 
     7501 AACAAACCAC CGCTGGTAGC GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA 
     7561 AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA 
     7621 ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT 
     7681 TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA 
     7741 GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA 
     7801 TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC 
     7861 CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA 
     7921 ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC 
     7981 AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA 
     8041 ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT 
     8101 TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG 
     8161 CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC 
     8221 TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT 
     8281 CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT 
     8341 GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC 
     8401 TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT 
     8461 CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA 
     8521 GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA 
     8581 CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG 
     8641 GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG 
     8701 TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG 
     8761 CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG 
     8821 CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC 
     8881 TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA 
     8941 AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC 
     9001 CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC 
     9061 TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT 
     9121 GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//