LOCUS       RG226287                9497 bp    DNA     circular  SYN 
DEFINITION  NLRP3 (tGFP-tagged) - Human NLR family, pyrin domain containing 3 (NLRP3), transcript variant 5
ACCESSION   NM_001127462
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3978..4682 
                     /label="GFP"  
     misc_feature    4729..5314 
                     /label="PolyA signal"  
     misc_feature    7406..8051 
                     /label="ColE1"  
     misc_feature    5768..6562 
                     /label="Neo"  
     misc_feature    5399..5701 
                     /label="SV40 ori"  
     misc_feature    8066..8926 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3957..3963 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3957 
                     /label="NLRP3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGATGGCA AGCACCCGCT GCAAGCTGGC CAGGTACCTG GAGGACCTGG 
     1081 AGGATGTGGA CTTGAAGAAA TTTAAGATGC ACTTAGAGGA CTATCCTCCC CAGAAGGGCT 
     1141 GCATCCCCCT CCCGAGGGGT CAGACAGAGA AGGCAGACCA TGTGGATCTA GCCACGCTAA 
     1201 TGATCGACTT CAATGGGGAG GAGAAGGCGT GGGCCATGGC CGTGTGGATC TTCGCTGCGA 
     1261 TCAACAGGAG AGACCTTTAT GAGAAAGCAA AAAGAGATGA GCCGAAGTGG GGTTCAGATA 
     1321 ATGCACGTGT TTCGAATCCC ACTGTGATAT GCCAGGAAGA CAGCATTGAA GAGGAGTGGA 
     1381 TGGGTTTACT GGAGTACCTT TCGAGAATCT CTATTTGTAA AATGAAGAAA GATTACCGTA 
     1441 AGAAGTACAG AAAGTACGTG AGAAGCAGAT TCCAGTGCAT TGAAGACAGG AATGCCCGTC 
     1501 TGGGTGAGAG TGTGAGCCTC AACAAACGCT ACACACGACT GCGTCTCATC AAGGAGCACC 
     1561 GGAGCCAGCA GGAGAGGGAG CAGGAGCTTC TGGCCATCGG CAAGACCAAG ACGTGTGAGA 
     1621 GCCCCGTGAG TCCCATTAAG ATGGAGTTGC TGTTTGACCC CGATGATGAG CATTCTGAGC 
     1681 CTGTGCACAC CGTGGTGTTC CAGGGGGCGG CAGGGATTGG GAAAACAATC CTGGCCAGGA 
     1741 AGATGATGTT GGACTGGGCG TCGGGGACAC TCTACCAAGA CAGGTTTGAC TATCTGTTCT 
     1801 ATATCCACTG TCGGGAGGTG AGCCTTGTGA CACAGAGGAG CCTGGGGGAC CTGATCATGA 
     1861 GCTGCTGCCC CGACCCAAAC CCACCCATCC ACAAGATCGT GAGAAAACCC TCCAGAATCC 
     1921 TCTTCCTCAT GGACGGCTTC GATGAGCTGC AAGGTGCCTT TGACGAGCAC ATAGGACCGC 
     1981 TCTGCACTGA CTGGCAGAAG GCCGAGCGGG GAGACATTCT CCTGAGCAGC CTCATCAGAA 
     2041 AGAAGCTGCT TCCCGAGGCC TCTCTGCTCA TCACCACGAG ACCTGTGGCC CTGGAGAAAC 
     2101 TGCAGCACTT GCTGGACCAT CCTCGGCATG TGGAGATCCT GGGTTTCTCC GAGGCCAAAA 
     2161 GGAAAGAGTA CTTCTTCAAG TACTTCTCTG ATGAGGCCCA AGCCAGGGCA GCCTTCAGTC 
     2221 TGATTCAGGA GAACGAGGTC CTCTTCACCA TGTGCTTCAT CCCCCTGGTC TGCTGGATCG 
     2281 TGTGCACTGG ACTGAAACAG CAGATGGAGA GTGGCAAGAG CCTTGCCCAG ACATCCAAGA 
     2341 CCACCACCGC GGTGTACGTC TTCTTCCTTT CCAGTTTGCT GCAGCCCCGG GGAGGGAGCC 
     2401 AGGAGCACGG CCTCTGCGCC CACCTCTGGG GGCTCTGCTC TTTGGCTGCA GATGGAATCT 
     2461 GGAACCAGAA AATCCTGTTT GAGGAGTCCG ACCTCAGGAA TCATGGACTG CAGAAGGCGG 
     2521 ATGTGTCTGC TTTCCTGAGG ATGAACCTGT TCCAAAAGGA AGTGGACTGC GAGAAGTTCT 
     2581 ACAGCTTCAT CCACATGACT TTCCAGGAGT TCTTTGCCGC CATGTACTAC CTGCTGGAAG 
     2641 AGGAAAAGGA AGGAAGGACG AACGTTCCAG GGAGTCGTTT GAAGCTTCCC AGCCGAGACG 
     2701 TGACAGTCCT TCTGGAAAAC TATGGCAAAT TCGAAAAGGG GTATTTGATT TTTGTTGTAC 
     2761 GTTTCCTCTT TGGCCTGGTA AACCAGGAGA GGACCTCCTA CTTGGAGAAG AAATTAAGTT 
     2821 GCAAGATCTC TCAGCAAATC AGGCTGGAGC TGCTGAAATG GATTGAAGTG AAAGCCAAAG 
     2881 CTAAAAAGCT GCAGATCCAG CCCAGCCAGC TGGAATTGTT CTACTGTTTG TACGAGATGC 
     2941 AGGAGGAGGA CTTCGTGCAA AGGGCCATGG ACTATTTCCC CAAGATTGAG ATCAATCTCT 
     3001 CCACCAGAAT GGACCACATG GTTTCTTCCT TTTGCATTGA GAACTGTCAT CGGGTGGAGT 
     3061 CACTGTCCCT GGGGTTTCTC CATAACATGC CCAAGGAGGA AGAGGAGGAG GAAAAGGAAG 
     3121 GCCGACACCT TGATATGGTG CAGTGTGTCC TCCCAAGCTC CTCTCATGCT GCCTGTTCTC 
     3181 ATGGGTTGGG GCGCTGTGGC CTCTCGCATG AGTGCTGCTT CGACATCTCC TTGGTCCTCA 
     3241 GCAGCAACCA GAAGCTGGTG GAGCTGGACC TGAGTGACAA CGCCCTCGGT GACTTCGGAA 
     3301 TCAGACTTCT GTGTGTGGGA CTGAAGCACC TGTTGTGCAA TCTGAAGAAG CTCTGGTTGG 
     3361 TCAGCTGCTG CCTCACATCA GCATGTTGTC AGGATCTTGC ATCAGTATTG AGCACCAGCC 
     3421 ATTCCCTGAC CAGACTCTAT GTGGGGGAGA ATGCCTTGGG AGACTCAGGA GTCGCAATTT 
     3481 TATGTGAAAA AGCCAAGAAT CCACAGTGTA ACCTGCAGAA ACTGGGGTTG GTGAATTCTG 
     3541 GCCTTACGTC AGTCTGTTGT TCAGCTTTGT CCTCGGTACT CAGCACTAAT CAGAATCTCA 
     3601 CGCACCTTTA CCTGCGAGGC AACACTCTCG GAGACAAGGG GATCAAACTA CTCTGTGAGG 
     3661 GACTCTTGCA CCCCGACTGC AAGCTTCAGG TGTTGGAATT AGACAACTGC AACCTCACGT 
     3721 CACACTGCTG CTGGGATCTT TCCACACTTC TGACCTCCAG CCAGAGCCTG CGAAAGCTGA 
     3781 GCCTGGGCAA CAATGACCTG GGCGACCTGG GGGTCATGAT GTTCTGTGAA GTGCTGAAAC 
     3841 AGCAGAGCTG CCTCCTGCAG AACCTGGGGT TGTCTGAAAT GTATTTCAAT TATGAGACAA 
     3901 AAAGTGCGTT AGAAACACTT CAAGAAGAAA AGCCTGAGCT GACCGTCGTC TTTGAGCCTT 
     3961 CTTGGACGCG TACGCGGCCG CTCGAGATGG AGAGCGACGA GAGCGGCCTG CCCGCCATGG 
     4021 AGATCGAGTG CCGCATCACC GGCACCCTGA ACGGCGTGGA GTTCGAGCTG GTGGGCGGCG 
     4081 GAGAGGGCAC CCCCGAGCAG GGCCGCATGA CCAACAAGAT GAAGAGCACC AAAGGCGCCC 
     4141 TGACCTTCAG CCCCTACCTG CTGAGCCACG TGATGGGCTA CGGCTTCTAC CACTTCGGCA 
     4201 CCTACCCCAG CGGCTACGAG AACCCCTTCC TGCACGCCAT CAACAACGGC GGCTACACCA 
     4261 ACACCCGCAT CGAGAAGTAC GAGGACGGCG GCGTGCTGCA CGTGAGCTTC AGCTACCGCT 
     4321 ACGAGGCCGG CCGCGTGATC GGCGACTTCA AGGTGATGGG CACCGGCTTC CCCGAGGACA 
     4381 GCGTGATCTT CACCGACAAG ATCATCCGCA GCAACGCCAC CGTGGAGCAC CTGCACCCCA 
     4441 TGGGCGATAA CGATCTGGAT GGCAGCTTCA CCCGCACCTT CAGCCTGCGC GACGGCGGCT 
     4501 ACTACAGCTC CGTGGTGGAC AGCCACATGC ACTTCAAGAG CGCCATCCAC CCCAGCATCC 
     4561 TGCAGAACGG GGGCCCCATG TTCGCCTTCC GCCGCGTGGA GGAGGATCAC AGCAACACCG 
     4621 AGCTGGGCAT CGTGGAGTAC CAGCACGCCT TCAAGACCCC GGATGCAGAT GCCGGTGAAG 
     4681 AAAGAGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4741 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4801 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4861 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4921 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4981 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     5041 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     5101 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     5161 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5221 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5281 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5341 ATGCCTGTTG AATTGGGTAC GCGGCCAGCT TGGCTGTGGA ATGTGTGTCA GTTAGGGTGT 
     5401 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA 
     5461 GCAACCAGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 
     5521 CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG 
     5581 CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC 
     5641 GAGGCCGCCT CGGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA 
     5701 GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA 
     5761 GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT 
     5821 TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC 
     5881 GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC 
     5941 GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC 
     6001 GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG 
     6061 GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC 
     6121 ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC 
     6181 CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT 
     6241 CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC 
     6301 AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG 
     6361 AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG 
     6421 GCCGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC 
     6481 GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC 
     6541 GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GAAATGACCG 
     6601 ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA 
     6661 GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC 
     6721 TCATGCTGGA GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT 
     6781 AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG 
     6841 GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA 
     6901 GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC 
     6961 CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT 
     7021 AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC 
     7081 AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT 
     7141 CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG 
     7201 CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA 
     7261 TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT 
     7321 TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC 
     7381 GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT 
     7441 CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG 
     7501 TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA 
     7561 AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT 
     7621 ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA 
     7681 ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA 
     7741 ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT 
     7801 TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTTTTT 
     7861 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT 
     7921 TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA 
     7981 GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA 
     8041 TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC 
     8101 CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA 
     8161 TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC 
     8221 CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA 
     8281 GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA 
     8341 GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG 
     8401 TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC 
     8461 GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG 
     8521 TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT 
     8581 CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT 
     8641 CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA 
     8701 ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC 
     8761 GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC 
     8821 CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA 
     8881 GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT 
     8941 TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT 
     9001 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC 
     9061 CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG 
     9121 TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC 
     9181 TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC 
     9241 GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA 
     9301 GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA 
     9361 ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG 
     9421 ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA 
     9481 AATTTAACGC GAATTTT
//