LOCUS       RG226279                9443 bp    DNA     circular  SYN 
DEFINITION  ASAP2 (tGFP-tagged) - Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (ASAP2), transcript variant 2
ACCESSION   NM_001135191
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3924..4628 
                     /label="GFP"  
     misc_feature    4675..5260 
                     /label="PolyA signal"  
     misc_feature    7352..7997 
                     /label="ColE1"  
     misc_feature    5714..6508 
                     /label="Neo"  
     misc_feature    5345..5647 
                     /label="SV40 ori"  
     misc_feature    8012..8872 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3903..3909 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3903 
                     /label="ASAP2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGGACCAG ATCTCCGTGT CGGAATTCGT GGCCGAGACC CATGAGGACT 
     1081 ACAAGGCGCC CACGGCCTCC AGCTTCACCA CCCGCACGGC GCAGTGCCGG AACACTGTGG 
     1141 CGGCCATCGA GGAGGCTTTG GACGTGGACC GGATGGTTCT TTACAAAATG AAGAAATCCG 
     1201 TGAAAGCAAT CAACAGCTCT GGGCTGGCTC ACGTGGAAAA TGAAGAGCAG TACACCCAGG 
     1261 CTCTGGAGAA GTTTGGCGGC AACTGTGTAT GCAGAGATGA CCCAGATTTA GGAAGTGCGT 
     1321 TCCTGAAGTT CTCAGTGTTT ACAAAGGAGT TGACAGCACT TTTCAAAAAC CTGATTCAGA 
     1381 ATATGAACAA CATAATCTCC TTCCCTTTGG ACAGTTTGCT GAAGGGGGAC CTGAAAGGAG 
     1441 TGAAAGGGGA TCTGAAAAAG CCTTTTGATA AAGCTTGGAA GGACTATGAA ACAAAAATAA 
     1501 CCAAGATAGA AAAGGAGAAA AAGGAACACG CCAAGCTCCA TGGGATGATT CGGACTGAAA 
     1561 TAAGCGGAGC GGAAATTGCC GAAGAGATGG AAAAGGAGAG GCGCTTCTTC CAGCTACAGA 
     1621 TGTGCGAGTA TCTGCTGAAG GTCAACGAAA TCAAGATTAA AAAGGGAGTA GATTTACTTC 
     1681 AGAATCTGAT CAAATACTTT CATGCCCAAT GCAATTTTTT TCAGGATGGA CTCAAAGCCG 
     1741 TGGAAAGCCT CAAACCTTCC ATTGAAACGC TGTCTACGGA TCTTCACACG ATCAAACAGG 
     1801 CCCAGGATGA AGAAAGAAGG CAGTTGATAC AGCTTCGAGA TATTTTGAAA TCCGCATTGC 
     1861 AGGTTGAACA GAAAGAGGAC TCCCAAATTC GTCAGAGCAC AGCTTATAGC TTACATCAGC 
     1921 CTCAGGGAAA CAAGGAACAT GGGACCGAGC GGAACGGCAG CCTCTACAAG AAGAGTGACG 
     1981 GGATCCGAAA AGTGTGGCAG AAAAGGAAAT GTTCAGTTAA AAATGGTTTT CTGACCATAT 
     2041 CCCATGGTAC CGCTAACCGG CCTCCTGCAA AGCTCAACCT GCTAACCTGC CAGGTGAAGA 
     2101 CCAACCCTGA GGAGAAGAAG TGCTTTGACC TCATTTCACA TGACAGAACT TACCACTTTC 
     2161 AAGCTGAAGA TGAACAGGAA TGTCAAATAT GGATGTCTGT GCTGCAAAAT AGCAAAGAAG 
     2221 AAGCTTTAAA CAATGCATTT AAGGGGGATG ACAATACTGG AGAAAATAAC ATCGTCCAAG 
     2281 AACTGACAAA GGAGATCATC TCAGAAGTGC AGAGGATGAC GGGCAATGAC GTCTGCTGTG 
     2341 ACTGTGGGGC GCCAGATCCT ACATGGCTTT CCACCAACCT GGGCATCCTG ACCTGCATCG 
     2401 AGTGTTCCGG AATCCACCGA GAGCTGGGGG TTCATTATTC CAGGATGCAG TCCCTGACCT 
     2461 TAGATGTACT GGGAACATCT GAGCTGCTGC TCGCCAAGAA TATTGGGAAT GCAGGCTTTA 
     2521 ATGAGATCAT GGAATGTTGC CTACCAGCTG AGGACTCAGT CAAACCCAAC CCAGGCAGCG 
     2581 ACATGAATGC AAGAAAGGAC TACATCACAG CCAAGTACAT CGAGAGGAGA TACGCAAGGA 
     2641 AGAAGCACGC GGATAACGCG GCGAAGCTTC ACAGTCTTTG CGAGGCCGTC AAAACGAGAG 
     2701 ATATTTTTGG ATTGCTCCAA GCTTATGCTG ATGGTGTGGA TCTTACGGAA AAAATCCCAC 
     2761 TGGCCAACGG ACATGAGCCG GATGAAACGG CCCTCCACCT TGCAGTCAGA TCCGTGGATC 
     2821 GAACCTCTCT TCACATTGTA GACTTTTTAG TTCAGAACAG TGGGAACCTG GATAAACAGA 
     2881 CAGGGAAAGG CAGCACAGCC CTGCACTACT GCTGCCTGAC CGACAATGCC GAGTGCCTCA 
     2941 AGTTGCTCCT GCGGGGGAAG GCCTCCATCG AGATAGCAAA CGAGTCAGGA GAGACTCCGC 
     3001 TGGACATTGC CAAGCGCCTC AAGCACGAGC ACTGTGAGGA GCTGCTGACC CAAGCCTTAT 
     3061 CTGGAAGATT TAATTCTCAC GTTCACGTTG AATATGAATG GCGACTACTC CACGAAGACC 
     3121 TGGATGAAAG TGATGACGAC ATGGATGAGA AATTGCAGCC CAGTCCCAAC CGGCGGGAAG 
     3181 ACCGGCCCAT CAGCTTCTAC CAGCTGGGCT CCAACCAGCT TCAGTCTAAC GCTGTATCTT 
     3241 TGGCCAGAGA TGCTGCAAAC CTTGCCAAGG AGAAGCAGAG GGCTTTCATG CCCAGCATCT 
     3301 TGCAGAATGA GACTTACGGA GCCCTCCTGA GTGGCAGCCC ACCTCCCGCC CAGCCTGCAG 
     3361 CCCCCAGCAC CACCAGCGCC CCCCCGCTTC CTCCACGGAA TGTTGGCAAA GATCCCCTGA 
     3421 CCCCCACGCC GCCCCCACCC GTTGCCAAGA CGCCCAGCGT AATGGAAGCC TTGAGCCAGC 
     3481 CGAGCAAGCC TGCCCCGCCT GGGATCTCAC AGATCAGGCC CCCACCTCTG CCCCCACAGC 
     3541 CGCCCAGCCG CCTCCCGCAG AAGAAGCCTG CGCCGGGGGC TGACAAGTCC ACCCCACTGA 
     3601 CCAACAAAGG CCAACCGAGA GGACCTGTGG ATCTCTCTGC AACGGAAGCT CTGGGTCCTC 
     3661 TGTCCAATGC TATGGTCCTG CAGCCCCCTG CACCCATGCC TAGGAAGTCG CAGGCAACCA 
     3721 AGTTGAAGCC TAAGCGGGTG AAAGCGCTCT ATAACTGTGT GGCTGACAAC CCCGATGAGC 
     3781 TCACCTTCTC CGAGGGGGAT GTGATCATCG TGGACGGGGA GGAGGACCAG GAGTGGTGGA 
     3841 TTGGCCACAT TGATGGAGAT CCTGGTCGCA AAGGCGCATT CCCGGTGTCA TTTGTGCACT 
     3901 TTATCGCTGA CACGCGTACG CGGCCGCTCG AGATGGAGAG CGACGAGAGC GGCCTGCCCG 
     3961 CCATGGAGAT CGAGTGCCGC ATCACCGGCA CCCTGAACGG CGTGGAGTTC GAGCTGGTGG 
     4021 GCGGCGGAGA GGGCACCCCC GAGCAGGGCC GCATGACCAA CAAGATGAAG AGCACCAAAG 
     4081 GCGCCCTGAC CTTCAGCCCC TACCTGCTGA GCCACGTGAT GGGCTACGGC TTCTACCACT 
     4141 TCGGCACCTA CCCCAGCGGC TACGAGAACC CCTTCCTGCA CGCCATCAAC AACGGCGGCT 
     4201 ACACCAACAC CCGCATCGAG AAGTACGAGG ACGGCGGCGT GCTGCACGTG AGCTTCAGCT 
     4261 ACCGCTACGA GGCCGGCCGC GTGATCGGCG ACTTCAAGGT GATGGGCACC GGCTTCCCCG 
     4321 AGGACAGCGT GATCTTCACC GACAAGATCA TCCGCAGCAA CGCCACCGTG GAGCACCTGC 
     4381 ACCCCATGGG CGATAACGAT CTGGATGGCA GCTTCACCCG CACCTTCAGC CTGCGCGACG 
     4441 GCGGCTACTA CAGCTCCGTG GTGGACAGCC ACATGCACTT CAAGAGCGCC ATCCACCCCA 
     4501 GCATCCTGCA GAACGGGGGC CCCATGTTCG CCTTCCGCCG CGTGGAGGAG GATCACAGCA 
     4561 ACACCGAGCT GGGCATCGTG GAGTACCAGC ACGCCTTCAA GACCCCGGAT GCAGATGCCG 
     4621 GTGAAGAAAG AGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4681 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4741 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4801 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4861 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4921 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4981 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5041 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5101 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5161 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5221 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5281 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCTTGGC TGTGGAATGT GTGTCAGTTA 
     5341 GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT 
     5401 TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC 
     5461 ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA 
     5521 ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA 
     5581 GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA 
     5641 GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA 
     5701 GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG 
     5761 GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT 
     5821 GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC 
     5881 CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG 
     5941 ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG 
     6001 CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA 
     6061 GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA 
     6121 TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT 
     6181 GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC 
     6241 AGGCTCAAGG CGCGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC 
     6301 TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG 
     6361 GGTGTGGCCG ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT 
     6421 GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG 
     6481 CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGAAA 
     6541 TGACCGACCA AGCGACGCCC AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT 
     6601 ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG 
     6661 GGGATCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT 
     6721 ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA 
     6781 GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA 
     6841 GCTAGAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA 
     6901 CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG 
     6961 TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT 
     7021 CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC 
     7081 GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG 
     7141 TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA 
     7201 AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG 
     7261 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA 
     7321 GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG 
     7381 TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG 
     7441 GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC 
     7501 GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG 
     7561 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA 
     7621 CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT 
     7681 GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG 
     7741 TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG 
     7801 GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT 
     7861 TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG 
     7921 TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA 
     7981 AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG 
     8041 AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG 
     8101 TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC 
     8161 GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG 
     8221 AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG 
     8281 AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG 
     8341 GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT 
     8401 CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC 
     8461 CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC 
     8521 ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA 
     8581 CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC 
     8641 GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT 
     8701 CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC 
     8761 GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA 
     8821 CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA 
     8881 TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT 
     8941 ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA 
     9001 AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC 
     9061 GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT 
     9121 CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG 
     9181 GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT 
     9241 CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT 
     9301 TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT 
     9361 CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT 
     9421 AACAAAAATT TAACGCGAAT TTT
//