LOCUS       RG226264                9344 bp    DNA     circular  SYN 
DEFINITION  DIS3 (tGFP-tagged) - Human DIS3 mitotic control homolog (S. cerevisiae) (DIS3), transcript variant 2
ACCESSION   NM_001128226
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3825..4529 
                     /label="GFP"  
     misc_feature    4576..5161 
                     /label="PolyA signal"  
     misc_feature    7253..7898 
                     /label="ColE1"  
     misc_feature    5615..6409 
                     /label="Neo"  
     misc_feature    5246..5548 
                     /label="SV40 ori"  
     misc_feature    7913..8773 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3804..3810 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3804 
                     /label="DIS3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTCAAGTCC AAGACGTTCT TAAAAAAGAC CCGGGCGGGC GGCGTGATGA 
     1081 AGATCGTGCG CGAGCACTAC CTGCGAGACG ACATCGGCTG CGGTGCGCCC GGGTGCGCAG 
     1141 CGTGTGGAGG GGCGCACGAG GGGCCGGCCC TGGAGCCGCA GCCCCAGGAC CCGGCGAGCA 
     1201 GCGTCTGCCC GCAACCGCAC TACTTGCTGC CCGACACTAA TGTGTTACTG CACCAGATTG 
     1261 TAAGTGCCTG GAGGCCGGGG ACCTGGGCTT CTGTGGCCTC CAGCCTGCGA CTCCCAGGCA 
     1321 GCTTAGAAAC CTATGTAGAA CAAGAACAGG GAGAAAATGC TAATGACAGG AATGATAGAG 
     1381 CGATTCGAGT AGCAGCAAAA TGGTACAATG AACATTTGAA AAAAATGTCA GCAGACAACC 
     1441 AGCTGCAAGT TATCTTCATA ACAAATGACA GGAGAAACAA AGAGAAAGCC ATAGAAGAAG 
     1501 GAATACCAGC TTTCACTTGT GAAGAATATG TAAAGAGCCT AACTGCTAAC CCCGAACTCA 
     1561 TAGATCGTCT TGCTTGTTTG TCTGAAGAAG GGAATGAAAT AGAAAGTGGA AAAATAATAT 
     1621 TTTCAGAGCA TCTTCCCTTA AGTAAGCTAC AGCAAGGCAT AAAATCTGGT ACATACCTTC 
     1681 AAGGAACATT TAGAGCTAGC AGGGAAAATT ACTTGGAAGC TACAGTATGG ATTCATGGCG 
     1741 ACAATGAAGA AAATAAAGAG ATAATCTTAC AGGGACTTAA ACATTTAAAC AGAGCTGTTC 
     1801 ACGAAGATAT TGTGGCTGTG GAGCTTCTCC CCAAGAGTCA GTGGGTAGCA CCATCTTCTG 
     1861 TGGTTTTACA TGATGAAGGT CAAAATGAAG AAGATGTGGA GAAAGAAGAA GAGACAGAAC 
     1921 GAATGCTTAA GACTGCTGTA AGCGAGAAAA TGTTGAAGCC TACAGGTAGA GTTGTAGGAA 
     1981 TAATAAAAAG GAATTGGAGA CCATATTGTG GCATGCTTTC CAAGTCTGAC ATTAAGGAGT 
     2041 CAAGAAGACA TCTCTTTACA CCTGCTGATA AGAGAATCCC TCGAATTCGC ATAGAAACCA 
     2101 GACAGGCTTC CACATTAGAA GGACGGAGAA TTATTGTTGC TATTGATGGT TGGCCCAGAA 
     2161 ATTCCAGATA TCCAAATGGA CACTTTGTGA GAAATTTAGG TGATGTTGGA GAGAAAGAGA 
     2221 CTGAAACAGA AGTTTTGTTA CTTGAACACG ATGTTCCCCA TCAGCCTTTT TCACAGGCTG 
     2281 TTCTTAGTTT TCTGCCAAAG ATGCCCTGGA GCATTACTGA AAAGGACATG AAAAACCGAG 
     2341 AAGACCTGAG GCATCTGTGT ATTTGTAGTG TAGACCCACC AGGATGTACT GATATAGACG 
     2401 ATGCTCTACA TTGTCGAGAA CTCGAAAATG GAAATTTGGA GGTTGGTGTT CATATTGCTG 
     2461 ATGTGAGCCA TTTTATTAGG CCAGGAAATG CCTTGGATCA AGAATCAGCC AGAAGAGGAA 
     2521 CAACTGTGTA TCTTTGTGAA AAGAGGATTG ACATGGTTCC AGAGTTGCTT AGCTCTAACT 
     2581 TGTGTTCCTT AAAATGTGAC GTGGACAGGC TGGCATTTTC ATGTATTTGG GAAATGAATC 
     2641 ACAATGCTGA AATCTTAAAA ACGAAGTTTA CCAAAAGTGT TATTAATTCA AAGGCATCTC 
     2701 TGACGTATGC TGAAGCTCAG TTGAGAATTG ATTCAGCAAA CATGAATGAT GATATTACCA 
     2761 CTAGTCTCCG TGGACTGAAT AAACTAGCCA AAATTCTGAA GAAAAGAAGG ATTGAAAAAG 
     2821 GGGCTTTGAC TCTATCCTCT CCTGAAGTTC GATTCCACAT GGACAGTGAA ACTCACGATC 
     2881 CTATAGATCT GCAGACCAAG GAACTTAGGG AAACAAATTC CATGGTTGAA GAATTTATGT 
     2941 TACTTGCCAA TATTTCTGTT GCAAAAAAAA TTCATGAGGA ATTTTCTGAA CATGCTCTGC 
     3001 TTCGAAAACA TCCTGCTCCA CCTCCATCAA ATTATGAAAT TCTTGTTAAG GCAGCCAGGT 
     3061 CAAGGAATTT GGAAATTAAG ACTGATACAG CCAAGTCTTT GGCTGAGTCT TTGGATCAGG 
     3121 CCGAATCTCC TACTTTTCCA TATCTAAACA CTCTGTTGAG AATATTAGCC ACTCGCTGTA 
     3181 TGATGCAAGC TGTGTACTTC TGTTCTGGAA TGGATAATGA TTTTCATCAC TATGGCTTAG 
     3241 CGTCTCCAAT ATACACACAT TTTACTTCAC CCATTAGAAG ATACGCAGAT GTCATTGTTC 
     3301 ATCGGCTTTT GGCTGTGGCT ATTGGGGCTG ACTGTACTTA TCCAGAGTTG ACAGACAAAC 
     3361 ACAAGCTTGC AGATATATGT AAAAATCTAA ATTTCCGGCA CAAAATGGCT CAATATGCCC 
     3421 AACGTGCATC AGTGGCTTTT CATACCCAGT TATTCTTCAA AAGCAAAGGA ATAGTAAGTG 
     3481 AAGAAGCCTA TATTTTATTT GTAAGAAAGA ATGCCATTGT GGTATTAATT CCAAAGTATG 
     3541 GTTTAGAAGG GACAGTCTTT TTTGAAGAAA AGGACAAACC AAACCCACAG CTTATTTATG 
     3601 ATGATGAGAT ACCCTCACTT AAAATAGAAG ATACAGTGTT CCATGTATTT GATAAAGTTA 
     3661 AAGTGAAAAT CATGTTAGAC TCATCTAATC TTCAACATCA GAAGATCCGA ATGTCCCTGG 
     3721 TAGAACCACA GATACCAGGA ATAAGCATTC CTACTGATAC TTCAAACATG GACCTTAATG 
     3781 GACCAAAGAA AAAGAAGATG AAGCTTGGAA AAACGCGTAC GCGGCCGCTC GAGATGGAGA 
     3841 GCGACGAGAG CGGCCTGCCC GCCATGGAGA TCGAGTGCCG CATCACCGGC ACCCTGAACG 
     3901 GCGTGGAGTT CGAGCTGGTG GGCGGCGGAG AGGGCACCCC CGAGCAGGGC CGCATGACCA 
     3961 ACAAGATGAA GAGCACCAAA GGCGCCCTGA CCTTCAGCCC CTACCTGCTG AGCCACGTGA 
     4021 TGGGCTACGG CTTCTACCAC TTCGGCACCT ACCCCAGCGG CTACGAGAAC CCCTTCCTGC 
     4081 ACGCCATCAA CAACGGCGGC TACACCAACA CCCGCATCGA GAAGTACGAG GACGGCGGCG 
     4141 TGCTGCACGT GAGCTTCAGC TACCGCTACG AGGCCGGCCG CGTGATCGGC GACTTCAAGG 
     4201 TGATGGGCAC CGGCTTCCCC GAGGACAGCG TGATCTTCAC CGACAAGATC ATCCGCAGCA 
     4261 ACGCCACCGT GGAGCACCTG CACCCCATGG GCGATAACGA TCTGGATGGC AGCTTCACCC 
     4321 GCACCTTCAG CCTGCGCGAC GGCGGCTACT ACAGCTCCGT GGTGGACAGC CACATGCACT 
     4381 TCAAGAGCGC CATCCACCCC AGCATCCTGC AGAACGGGGG CCCCATGTTC GCCTTCCGCC 
     4441 GCGTGGAGGA GGATCACAGC AACACCGAGC TGGGCATCGT GGAGTACCAG CACGCCTTCA 
     4501 AGACCCCGGA TGCAGATGCC GGTGAAGAAA GAGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4561 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4621 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4681 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4741 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4801 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4861 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4921 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4981 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5041 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5101 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5161 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCTTGG 
     5221 CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 
     5281 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA 
     5341 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA 
     5401 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA 
     5461 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG 
     5521 TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA 
     5581 TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT 
     5641 GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA 
     5701 CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG 
     5761 GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG 
     5821 CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT 
     5881 GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT 
     5941 CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG 
     6001 CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT 
     6061 ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC 
     6121 GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC 
     6181 GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA 
     6241 TTCATCGACT GTGGCCGGCT GGGTGTGGCC GACCGCTATC AGGACATAGC GTTGGCTACC 
     6301 CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT 
     6361 ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA 
     6421 GCGGGACTCT GGGGTTCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT 
     6481 TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG 
     6541 GCTGGATGAT CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT 
     6601 TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG 
     6661 CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG 
     6721 TCTGTATACC GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG 
     6781 TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA 
     6841 AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG 
     6901 CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA 
     6961 GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG 
     7021 TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG 
     7081 AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC 
     7141 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA 
     7201 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT 
     7261 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC 
     7321 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC 
     7381 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC 
     7441 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT 
     7501 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG 
     7561 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA 
     7621 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA 
     7681 AACAAACCAC CGCTGGTAGC GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA 
     7741 AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA 
     7801 ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT 
     7861 TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA 
     7921 GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA 
     7981 TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC 
     8041 CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA 
     8101 ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC 
     8161 AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA 
     8221 ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT 
     8281 TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG 
     8341 CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC 
     8401 TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT 
     8461 CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT 
     8521 GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC 
     8581 TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT 
     8641 CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA 
     8701 GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA 
     8761 CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG 
     8821 GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG 
     8881 TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG 
     8941 CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG 
     9001 CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC 
     9061 TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA 
     9121 AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC 
     9181 CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC 
     9241 TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT 
     9301 GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//