LOCUS       RG226254                9278 bp    DNA     circular  SYN 
DEFINITION  GRM1 (tGFP-tagged) - Human glutamate receptor, metabotropic 1 (GRM1), transcript variant 2
ACCESSION   NM_001114329
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3759..4463 
                     /label="GFP"  
     misc_feature    4510..5095 
                     /label="PolyA signal"  
     misc_feature    7187..7832 
                     /label="ColE1"  
     misc_feature    5549..6343 
                     /label="Neo"  
     misc_feature    5180..5482 
                     /label="SV40 ori"  
     misc_feature    7847..8707 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3738..3744 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3738 
                     /label="GRM1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTCGGGCTC CTTTTGTTTT TTTTCCCAGC GATCTTTTTG GAGGTGTCCC 
     1081 TTCTCCCCAG AAGCCCCGGC AGGAAAGTGT TGCTGGCAGG AGCGTCGTCT CAGCGCTCGG 
     1141 TGGCCAGAAT GGACGGAGAT GTCATCATTG GAGCCCTCTT CTCAGTCCAT CACCAGCCTC 
     1201 CGGCCGAGAA AGTGCCCGAG AGGAAGTGTG GGGAGATCAG GGAGCAGTAT GGCATCCAGA 
     1261 GGGTGGAGGC CATGTTCCAC ACGTTGGATA AGATCAACGC GGACCCGGTC CTCCTGCCCA 
     1321 ACATCACCCT GGGCAGTGAG ATCCGGGACT CCTGCTGGCA CTCTTCCGTG GCTCTGGAAC 
     1381 AGAGCATTGA GTTCATTAGG GACTCTCTGA TTTCCATTCG AGATGAGAAG GATGGGATCA 
     1441 ACCGGTGTCT GCCTGACGGC CAGTCCCTCC CCCCAGGCAG GACTAAGAAG CCCATTGCGG 
     1501 GAGTGATCGG TCCCGGCTCC AGCTCTGTAG CCATTCAAGT GCAGAACCTG CTCCAGCTCT 
     1561 TCGACATCCC CCAGATCGCT TATTCAGCCA CAAGCATCGA CCTGAGTGAC AAAACTTTGT 
     1621 ACAAATACTT CCTGAGGGTT GTCCCTTCTG ACACTTTGCA GGCAAGGGCC ATGCTTGACA 
     1681 TAGTCAAACG TTACAATTGG ACCTATGTCT CTGCAGTCCA CACGGAAGGG AATTATGGGG 
     1741 AGAGCGGAAT GGACGCTTTC AAAGAGCTGG CTGCCCAGGA AGGCCTCTGT ATCGCCCATT 
     1801 CTGACAAAAT CTACAGCAAC GCTGGGGAGA AGAGCTTTGA CCGACTCTTG CGCAAACTCC 
     1861 GAGAGAGGCT TCCCAAGGCT AGAGTGGTGG TCTGCTTCTG TGAAGGCATG ACAGTGCGAG 
     1921 GACTCCTGAG CGCCATGCGG CGCCTTGGCG TCGTGGGCGA GTTCTCACTC ATTGGAAGTG 
     1981 ATGGATGGGC AGACAGAGAT GAAGTCATTG AAGGTTATGA GGTGGAAGCC AACGGGGGAA 
     2041 TCACGATAAA GCTGCAGTCT CCAGAGGTCA GGTCATTTGA TGATTATTTC CTGAAACTGA 
     2101 GGCTGGACAC TAACACGAGG AATCCCTGGT TCCCTGAGTT CTGGCAACAT CGGTTCCAGT 
     2161 GCCGCCTTCC AGGACACCTT CTGGAAAATC CCAACTTTAA ACGAATCTGC ACAGGCAATG 
     2221 AAAGCTTAGA AGAAAACTAT GTCCAGGACA GTAAGATGGG GTTTGTCATC AATGCCATCT 
     2281 ATGCCATGGC ACATGGGCTG CAGAACATGC ACCATGCCCT CTGCCCTGGC CACGTGGGCC 
     2341 TCTGCGATGC CATGAAGCCC ATCGACGGCA GCAAGCTGCT GGACTTCCTC ATCAAGTCCT 
     2401 CATTCATTGG AGTATCTGGA GAGGAGGTGT GGTTTGATGA GAAAGGAGAC GCTCCTGGAA 
     2461 GGTATGATAT CATGAATCTG CAGTACACTG AAGCTAATCG CTATGACTAT GTGCACGTTG 
     2521 GAACCTGGCA TGAAGGAGTG CTGAACATTG ATGATTACAA AATCCAGATG AACAAGAGTG 
     2581 GAGTGGTGCG GTCTGTGTGC AGTGAGCCTT GCTTAAAGGG CCAGATTAAG GTTATACGGA 
     2641 AAGGAGAAGT GAGCTGCTGC TGGATTTGCA CGGCCTGCAA AGAGAATGAA TATGTGCAAG 
     2701 ATGAGTTCAC CTGCAAAGCT TGTGACTTGG GATGGTGGCC CAATGCAGAT CTAACAGGCT 
     2761 GTGAGCCCAT TCCTGTGCGC TATCTTGAGT GGAGCAACAT CGAATCCATT ATAGCCATCG 
     2821 CCTTTTCATG CCTGGGAATC CTTGTTACCT TGTTTGTCAC CCTAATCTTT GTACTGTACC 
     2881 GGGACACACC AGTGGTCAAA TCCTCCAGTC GGGAGCTCTG CTACATCATC CTAGCTGGCA 
     2941 TCTTCCTTGG TTATGTGTGC CCATTCACTC TCATTGCCAA ACCTACTACC ACCTCCTGCT 
     3001 ACCTCCAGCG CCTCTTGGTT GGCCTCTCCT CTGCGATGTG CTACTCTGCT TTAGTGACTA 
     3061 AAACCAATCG TATTGCACGC ATCCTGGCTG GCAGCAAGAA GAAGATCTGC ACCCGGAAGC 
     3121 CCAGGTTCAT GAGTGCCTGG GCTCAGGTGA TCATTGCCTC AATTCTGATT AGTGTGCAAC 
     3181 TAACCCTGGT GGTAACCCTG ATCATCATGG AACCCCCTAT GCCCATTCTG TCCTACCCAA 
     3241 GTATCAAGGA AGTCTACCTT ATCTGCAATA CCAGCAACCT GGGTGTGGTG GCCCCTTTGG 
     3301 GCTACAATGG ACTCCTCATC ATGAGCTGTA CCTACTATGC CTTCAAGACC CGCAACGTGC 
     3361 CCGCCAACTT CAACGAGGCC AAATATATCG CGTTCACCAT GTACACCACC TGTATCATCT 
     3421 GGCTAGCTTT TGTGCCCATT TACTTTGGGA GCAACTACAA GATCATCACA ACTTGCTTTG 
     3481 CAGTGAGTCT CAGTGTAACA GTGGCTCTGG GGTGCATGTT CACTCCCAAG ATGTACATCA 
     3541 TTATTGCCAA GCCTGAGAGG AATGTCCGCA GTGCCTTCAC CACCTCTGAT GTTGTCCGCA 
     3601 TGCATGTTGG CGATGGCAAG CTGCCCTGCC GCTCCAACAC TTTCCTCAAC ATCTTCCGAA 
     3661 GAAAGAAGGC AGGGGCAGGG AATGCCAAGA AGAGGCAGCC AGAATTCTCG CCCACCAGCC 
     3721 AATGTCCGTC GGCACATGTG CAGCTTACGC GTACGCGGCC GCTCGAGATG GAGAGCGACG 
     3781 AGAGCGGCCT GCCCGCCATG GAGATCGAGT GCCGCATCAC CGGCACCCTG AACGGCGTGG 
     3841 AGTTCGAGCT GGTGGGCGGC GGAGAGGGCA CCCCCGAGCA GGGCCGCATG ACCAACAAGA 
     3901 TGAAGAGCAC CAAAGGCGCC CTGACCTTCA GCCCCTACCT GCTGAGCCAC GTGATGGGCT 
     3961 ACGGCTTCTA CCACTTCGGC ACCTACCCCA GCGGCTACGA GAACCCCTTC CTGCACGCCA 
     4021 TCAACAACGG CGGCTACACC AACACCCGCA TCGAGAAGTA CGAGGACGGC GGCGTGCTGC 
     4081 ACGTGAGCTT CAGCTACCGC TACGAGGCCG GCCGCGTGAT CGGCGACTTC AAGGTGATGG 
     4141 GCACCGGCTT CCCCGAGGAC AGCGTGATCT TCACCGACAA GATCATCCGC AGCAACGCCA 
     4201 CCGTGGAGCA CCTGCACCCC ATGGGCGATA ACGATCTGGA TGGCAGCTTC ACCCGCACCT 
     4261 TCAGCCTGCG CGACGGCGGC TACTACAGCT CCGTGGTGGA CAGCCACATG CACTTCAAGA 
     4321 GCGCCATCCA CCCCAGCATC CTGCAGAACG GGGGCCCCAT GTTCGCCTTC CGCCGCGTGG 
     4381 AGGAGGATCA CAGCAACACC GAGCTGGGCA TCGTGGAGTA CCAGCACGCC TTCAAGACCC 
     4441 CGGATGCAGA TGCCGGTGAA GAAAGAGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4501 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4561 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4621 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4681 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4741 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4801 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4861 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     4921 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     4981 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5041 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5101 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC TTGGCTGTGG 
     5161 AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA 
     5221 AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC 
     5281 AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG 
     5341 CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT 
     5401 TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG AGCTATTCCA GAAGTAGTGA 
     5461 GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT 
     5521 TTCGGATCTG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG 
     5581 CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG 
     5641 ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT 
     5701 TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA 
     5761 TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG 
     5821 GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT 
     5881 GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT 
     5941 CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG 
     6001 ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA 
     6061 GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC 
     6121 CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC 
     6181 GACTGTGGCC GGCTGGGTGT GGCCGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT 
     6241 ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC 
     6301 GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA 
     6361 CTCTGGGGTT CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA GATTTCGATT 
     6421 CCACCGCCGC CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA 
     6481 TGATCCTCCA GCGCGGGGAT CTCATGCTGG AGTTCTTCGC CCACCCCAAC TTGTTTATTG 
     6541 CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT 
     6601 TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGTA 
     6661 TACCGTCGAC CTCTAGCTAG AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA 
     6721 ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT 
     6781 GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC 
     6841 AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG 
     6901 GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC 
     6961 GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG 
     7021 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA 
     7081 AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC 
     7141 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC 
     7201 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG 
     7261 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT 
     7321 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC 
     7381 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC 
     7441 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG 
     7501 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG 
     7561 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA 
     7621 CCACCGCTGG TAGCGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT 
     7681 CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC 
     7741 GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT 
     7801 AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC 
     7861 AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG 
     7921 CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG 
     7981 CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC 
     8041 CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA 
     8101 TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG 
     8161 TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT 
     8221 CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA 
     8281 GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG 
     8341 TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA 
     8401 CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT 
     8461 GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA 
     8521 TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT 
     8581 CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT 
     8641 CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA 
     8701 AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT 
     8761 GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC 
     8821 GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG 
     8881 GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT 
     8941 TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC 
     9001 GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG 
     9061 ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA 
     9121 CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC 
     9181 CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA 
     9241 AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//