LOCUS       RG226248                9263 bp    DNA     circular  SYN 
DEFINITION  SLFN11 (tGFP-tagged) - Human schlafen family member 11 (SLFN11), transcript variant 2
ACCESSION   NM_001104588
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3744..4448 
                     /label="GFP"  
     misc_feature    4495..5080 
                     /label="PolyA signal"  
     misc_feature    7172..7817 
                     /label="ColE1"  
     misc_feature    5534..6328 
                     /label="Neo"  
     misc_feature    5165..5467 
                     /label="SV40 ori"  
     misc_feature    7832..8692 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3723..3729 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3723 
                     /label="SLFN11"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGCAAAT CAGTGCCCCC TGGTTGTGGA ACCATCTTAC CCAGACCTGG 
     1081 TCATCAATGT AGGAGAAGTG ACTCTTGGAG AAGAAAACAG AAAAAAGCTG CAGAAAATTC 
     1141 AGAGAGACCA AGAGAAGGAG AGAGTTATGC GGGCTGCATG TGCTTTATTA AACTCAGGAG 
     1201 GAGGAGTGAT TCGAATGGCC AAGAAGGTTG AGCATCCCGT GGAGATGGGA CTGGATTTAG 
     1261 AACAGTCTTT GAGAGAGCTT ATTCAGTCTT CAGATCTGCA GGCTTTCTTT GAGACCAAGC 
     1321 AACAAGGAAG GTGTTTTTAC ATTTTTGTTA AATCTTGGAG CAGTGGCCCT TTCCCTGAAG 
     1381 ATCGCTCTGT CAAGCCCCGC CTTTGCAGCC TCAGTTCTTC ATTATACCGT AGATCTGAGA 
     1441 CCTCTGTGCG TTCCATGGAC TCAAGAGAGG CATTCTGTTT CCTGAAGACC AAAAGGAAGC 
     1501 CAAAAATCTT GGAAGAAGGA CCTTTTCACA AAATTCACAA GGGTGTATAC CAAGAGCTCC 
     1561 CTAACTCGGA TCCTGCTGAC CCAAACTCGG ATCCTGCTGA CCTAATTTTC CAAAAAGACT 
     1621 ATCTTGAATA TGGTGAAATC CTGCCTTTTC CTGAGTCTCA GTTAGTAGAG TTTAAACAGT 
     1681 TCTCTACAAA ACACTTCCAA GAATATGTAA AAAGGACAAT TCCAGAATAC GTCCCTGCAT 
     1741 TTGCAAACAC TGGAGGAGGC TATCTTTTTA TTGGAGTGGA TGATAAGAGT AGGGAAGTCC 
     1801 TGGGATGTGC AAAAGAAAAT GTTGACCCTG ACTCTTTGAG AAGGAAAATA GAACAAGCCA 
     1861 TATACAAACT ACCTTGTGTT CATTTTTGCC AACCCCAACG CCCGATAACC TTCACACTCA 
     1921 AAATTGTGAA TGTGTTAAAA AGGGGAGAGC TCTATGGCTA TGCTTGCATG ATCAGAGTAA 
     1981 ATCCCTTCTG CTGTGCAGTG TTCTCAGAAG CTCCCAATTC ATGGATAGTG GAGGACAAGT 
     2041 ACGTCTGCAG CCTGACAACC GAGAAATGGG TAGGCATGAT GACAGACACA GATCCAGATC 
     2101 TTCTACAGTT GTCTGAAGAT TTTGAATGTC AGCTGAGTCT ATCTAGTGGG CCTCCCCTTA 
     2161 GCAGACCAGT GTACTCCAAG AAAGGCCTGG AACATAAAAA GGAACTCCAG CAACTTTTAT 
     2221 TTTCAGTCCC ACCAGGATAT TTGCGATATA CTCCAGAGTC ACTCTGGAGG GACCTGATCT 
     2281 CAGAGCACAG AGGACTAGAG GAGTTAATAA ATAAGCAAAT GCAACCTTTC TTTCGGGGAA 
     2341 TTTTGATCTT CTCTAGAAGT TGGGCTGTGG ACCTGAACTT GCAGGAGAAG CCAGGAGTCA 
     2401 TCTGTGATGC TCTGCTGATA GCACAGAACA GCACCCCCAT TCTCTACACC ATTCTCAGGG 
     2461 AGCAAGATGC AGAGGGCCAG GACTACTGCA CTCGCACCGC CTTTACTTTG AAGCAGAAGC 
     2521 TAGTGAACAT GGGGGGCTAC ACCGGGAAGG TGTGTGTCAG GGCCAAGGTC CTCTGCCTGA 
     2581 GTCCTGAGAG CAGCGCAGAG GCCTTGGAGG CTGCAGTGTC TCCGATGGAT TACCCTGCGT 
     2641 CCTATAGCCT TGCAGGCACC CAGCACATGG AAGCCCTGCT GCAGTCCCTC GTGATTGTCT 
     2701 TACTCGGCTT CAGGTCTCTC TTGAGTGACC AGCTCGGCTG TGAGGTTTTA AATCTGCTCA 
     2761 CAGCCCAGCA GTATGAGATA TTCTCCAGAA GCCTCCGCAA GAACAGAGAG TTGTTTGTCC 
     2821 ACGGCTTACC TGGCTCAGGG AAGACCATCA TGGCCATGAA GATCATGGAG AAGATCAGGA 
     2881 ATGTGTTTCA CTGTGAGGCA CACAGAATTC TCTACGTTTG TGAAAACCAG CCTCTGAGGA 
     2941 ACTTTATCAG TGATAGAAAT ATCTGCCGAG CAGAGACCCG GAAAACTTTC CTAAGAGAAA 
     3001 ACTTTGAACA CATTCAACAC ATCGTCATTG ACGAAGCTCA GAATTTCCGT ACTGAAGATG 
     3061 GGGACTGGTA TGGGAAGGCA AAAAGCATCA CTCGGAGAGC AAAGGGTGGC CCAGGAATTC 
     3121 TCTGGATCTT TCTGGATTAC TTTCAGACCA GCCACTTGGA TTGCAGTGGC CTCCCTCCTC 
     3181 TCTCAGACCA ATATCCAAGA GAAGAGCTCA CCAGAATAGT TCGCAATGCA GATCCAATAG 
     3241 CCAAGTACTT ACAAAAAGAA ATGCAAGTAA TTAGAAGTAA TCCTTCATTT AACATCCCCA 
     3301 CTGGGTGCCT CGAGGTATTT CCTGAAGCCG AATGGTCCCA GGGTGTTCAG GGAACCTTAC 
     3361 GAATTAAGAA ATACTTGACT GTGGAGCAAA TAATGACCTG TGTGGCAGAC ACGTGCAGGC 
     3421 GCTTCTTTGA TAGGGGCTAT TCTCCAAAGG ATGTTGCTGT GCTTGTCAGC ACCGCAAAAG 
     3481 AAGTGGAGCA CTATAAGTAT GAGCTCTTGA AAGCAATGAG GAAGAAAAGG GTGGTGCAGC 
     3541 TCAGTGATGC ATGTGATATG TTGGGTGATC ACATTGTGTT GGACAGTGTT CGGCGATTCT 
     3601 CAGGCCTGGA AAGGAGCATA GTGTTTGGGA TCCATCCAAG GACAGCTGAC CCAGCTATCT 
     3661 TACCCAATGT TCTGATCTGT CTGGCTTCCA GGGCAAAACA ACACCTGTAT ATTTTTCCGT 
     3721 GGGGTGGCCA TACGCGTACG CGGCCGCTCG AGATGGAGAG CGACGAGAGC GGCCTGCCCG 
     3781 CCATGGAGAT CGAGTGCCGC ATCACCGGCA CCCTGAACGG CGTGGAGTTC GAGCTGGTGG 
     3841 GCGGCGGAGA GGGCACCCCC GAGCAGGGCC GCATGACCAA CAAGATGAAG AGCACCAAAG 
     3901 GCGCCCTGAC CTTCAGCCCC TACCTGCTGA GCCACGTGAT GGGCTACGGC TTCTACCACT 
     3961 TCGGCACCTA CCCCAGCGGC TACGAGAACC CCTTCCTGCA CGCCATCAAC AACGGCGGCT 
     4021 ACACCAACAC CCGCATCGAG AAGTACGAGG ACGGCGGCGT GCTGCACGTG AGCTTCAGCT 
     4081 ACCGCTACGA GGCCGGCCGC GTGATCGGCG ACTTCAAGGT GATGGGCACC GGCTTCCCCG 
     4141 AGGACAGCGT GATCTTCACC GACAAGATCA TCCGCAGCAA CGCCACCGTG GAGCACCTGC 
     4201 ACCCCATGGG CGATAACGAT CTGGATGGCA GCTTCACCCG CACCTTCAGC CTGCGCGACG 
     4261 GCGGCTACTA CAGCTCCGTG GTGGACAGCC ACATGCACTT CAAGAGCGCC ATCCACCCCA 
     4321 GCATCCTGCA GAACGGGGGC CCCATGTTCG CCTTCCGCCG CGTGGAGGAG GATCACAGCA 
     4381 ACACCGAGCT GGGCATCGTG GAGTACCAGC ACGCCTTCAA GACCCCGGAT GCAGATGCCG 
     4441 GTGAAGAAAG AGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4501 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4561 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4621 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4681 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4741 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4801 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4861 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4921 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4981 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5041 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5101 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCTTGGC TGTGGAATGT GTGTCAGTTA 
     5161 GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT 
     5221 TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC 
     5281 ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA 
     5341 ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA 
     5401 GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA 
     5461 GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA 
     5521 GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG 
     5581 GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT 
     5641 GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC 
     5701 CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG 
     5761 ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG 
     5821 CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA 
     5881 GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA 
     5941 TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT 
     6001 GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC 
     6061 AGGCTCAAGG CGCGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC 
     6121 TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG 
     6181 GGTGTGGCCG ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT 
     6241 GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG 
     6301 CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGAAA 
     6361 TGACCGACCA AGCGACGCCC AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT 
     6421 ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG 
     6481 GGGATCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT 
     6541 ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA 
     6601 GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA 
     6661 GCTAGAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA 
     6721 CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG 
     6781 TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT 
     6841 CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC 
     6901 GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG 
     6961 TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA 
     7021 AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG 
     7081 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA 
     7141 GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG 
     7201 TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG 
     7261 GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC 
     7321 GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG 
     7381 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA 
     7441 CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT 
     7501 GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG 
     7561 TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG 
     7621 GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT 
     7681 TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG 
     7741 TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA 
     7801 AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG 
     7861 AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG 
     7921 TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC 
     7981 GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG 
     8041 AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG 
     8101 AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG 
     8161 GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT 
     8221 CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC 
     8281 CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC 
     8341 ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA 
     8401 CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC 
     8461 GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT 
     8521 CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC 
     8581 GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA 
     8641 CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA 
     8701 TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT 
     8761 ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA 
     8821 AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC 
     8881 GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT 
     8941 CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG 
     9001 GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT 
     9061 CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT 
     9121 TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT 
     9181 CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT 
     9241 AACAAAAATT TAACGCGAAT TTT
//