LOCUS       RG226235                9218 bp    DNA     circular  SYN 
DEFINITION  PDE4A (tGFP-tagged) - Human phosphodiesterase 4A, cAMP-specific (PDE4A), transcript variant 1
ACCESSION   NM_001111307
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3699..4403 
                     /label="GFP"  
     misc_feature    4450..5035 
                     /label="PolyA signal"  
     misc_feature    7127..7772 
                     /label="ColE1"  
     misc_feature    5489..6283 
                     /label="Neo"  
     misc_feature    5120..5422 
                     /label="SV40 ori"  
     misc_feature    7787..8647 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3678..3684 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3678 
                     /label="PDE4A"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAACCCCCG ACCGTCCCCT CGGAAAGGAG CCTGTCTCTG TCACTGCCCG 
     1081 GGCCCCGGGA GGGCCAGGCC ACCCTGAAGC CTCCCCCGCA GCACCTGTGG CGGCAGCCTC 
     1141 GGACCCCCAT CCGTATCCAG CAGCGCGGCT ACTCCGACAG CGCGGAGCGC GCCGAGCGGG 
     1201 AGCGGCAGCC GCACCGGCCC ATAGAGCGCG CCGATGCCAT GGACACCAGC GACCGGCCCG 
     1261 GCCTGCGCAC GACCCGCATG TCCTGGCCCT CGTCCTTCCA TGGCACTGGC ACCGGCAGCG 
     1321 GCGGCGCGGG CGGAGGCAGC AGCAGGCGCT TCGAGGCAGA GAATGGGCCG ACACCATCTC 
     1381 CTGGCCGCAG CCCCCTGGAC TCGCAGGCGA GCCCAGGACT CGTGCTGCAC GCCGGGGCGG 
     1441 CCACCAGCCA GCGCCGGGAG TCCTTCCTGT ACCGCTCAGA CAGCGACTAT GACATGTCAC 
     1501 CCAAGACCAT GTCCCGGAAC TCATCGGTCA CCAGCGAGGC GCACGCTGAA GACCTCATCG 
     1561 TAACACCATT TGCTCAGGTG CTGGCCAGCC TCCGGAGCGT CCGTAGCAAC TTCTCACTCC 
     1621 TGACCAATGT GCCCGTTCCC AGTAACAAGC GGTCCCCGCT GGGCGGCCCC ACCCCTGTCT 
     1681 GCAAGGCCAC GCTGTCAGAA GAAACGTGTC AGCAGTTGGC CCGGGAGACT CTGGAGGAGC 
     1741 TGGACTGGTG TCTGGAGCAG CTGGAGACCA TGCAGACCTA TCGCTCTGTC AGCGAGATGG 
     1801 CCTCGCACAA GTTCAAAAGG ATGTTGAACC GTGAGCTCAC ACACCTGTCA GAAATGAGCA 
     1861 GGTCCGGAAA CCAGGTCTCA GAGTACATTT CCACAACATT CCTGGACAAA CAGAATGAAG 
     1921 TGGAGATCCC ATCACCCACG ATGAAGGAAC GAGAAAAACA GCAAGCGCCG CGACCAAGAC 
     1981 CCTCCCAGCC GCCCCCGCCC CCTGTACCAC ACTTACAGCC CATGTCCCAA ATCACAGGGT 
     2041 TGAAAAAGTT GATGCATAGT AACAGCCTGA ACAACTCTAA CATTCCCCGA TTTGGGGTGA 
     2101 AGACCGATCA AGAAGAGCTC CTGGCCCAAG AACTGGAGAA CCTGAACAAG TGGGGCCTGA 
     2161 ACATCTTTTG CGTGTCGGAT TACGCTGGAG GCCGCTCACT CACCTGCATC ATGTACATGA 
     2221 TATTCCAGGA GCGGGACCTG CTGAAGAAAT TCCGCATCCC TGTGGACACG ATGGTGACAT 
     2281 ACATGCTGAC GCTGGAGGAT CACTACCACG CTGACGTGGC CTACCATAAC AGCCTGCACG 
     2341 CAGCTGACGT GCTGCAGTCC ACCCACGTAC TGCTGGCCAC GCCTGCACTA GATGCAGTGT 
     2401 TCACGGACCT GGAGATTCTC GCCGCCCTCT TCGCGGCTGC CATCCACGAT GTGGATCACC 
     2461 CTGGGGTCTC CAACCAGTTC CTCATCAACA CCAATTCGGA GCTGGCGCTC ATGTACAACG 
     2521 ATGAGTCGGT GCTCGAGAAT CACCACCTGG CCGTGGGCTT CAAGCTGCTG CAGGAGGACA 
     2581 ACTGCGACAT CTTCCAGAAC CTCAGCAAGC GCCAGCGGCA GAGCCTACGC AAGATGGTCA 
     2641 TCGACATGGT GCTGGCCACG GACATGTCCA AGCACATGAC CCTCCTGGCT GACCTGAAGA 
     2701 CCATGGTGGA GACCAAGAAA GTGACCAGCT CAGGGGTCCT CCTGCTAGAT AACTACTCCG 
     2761 ACCGCATCCA GGTCCTCCGG AACATGGTGC ACTGTGCCGA CCTCAGCAAC CCCACCAAGC 
     2821 CGCTGGAGCT GTACCGCCAG TGGACAGACC GCATCATGGC CGAGTTCTTC CAGCAGGGTG 
     2881 ACCGAGAGCG CGAGCGTGGC ATGGAAATCA GCCCCATGTG TGACAAGCAC ACTGCCTCCG 
     2941 TGGAGAAGTC TCAGGTGGGT TTTATTGACT ACATTGTGCA CCCATTGTGG GAGACCTGGG 
     3001 CGGACCTTGT CCACCCAGAT GCCCAGGAGA TCTTGGACAC TTTGGAGGAC AACCGGGACT 
     3061 GGTACTACAG CGCCATCCGG CAGAGCCCAT CTCCGCCACC CGAGGAGGAG TCAAGGGGGC 
     3121 CAGGCCACCC ACCCCTGCCT GACAAGTTCC AGTTTGAGCT GACGCTGGAG GAGGAAGAGG 
     3181 AGGAAGAAAT ATCAATGGCC CAGATACCGT GCACAGCCCA AGAGGCATTG ACTGCGCAGG 
     3241 GATTGTCAGG AGTCGAGGAA GCTCTGGATG CAACCATAGC CTGGGAGGCA TCCCCGGCCC 
     3301 AGGAGTCGTT GGAAGTTATG GCACAGGAAG CATCCCTGGA GGCCGAGCTG GAGGCAGTGT 
     3361 ATTTGACACA GCAGGCACAG TCCACAGGCA GTGCACCTGT GGCTCCGGAT GAGTTCTCGT 
     3421 CCCGGGAGGA ATTCGTGGTT GCTGTAAGCC ACAGCAGCCC CTCTGCCCTG GCTCTTCAAA 
     3481 GCCCCCTTCT CCCTGCTTGG AGGACCCTGT CTGTTTCAGA GCATGCCCCG GGCCTCCCGG 
     3541 GCCTCCCCTC CACGGCGGCC GAGGTGGAGG CCCAACGAGA GCACCAGGCT GCCAAGAGGG 
     3601 CTTGCAGTGC CTGCGCAGGG ACATTTGGGG AGGACACATC CGCACTCCCA GCTCCTGGTG 
     3661 GCGGGGGGTC AGGTGGAGAC CCTACCACGC GTACGCGGCC GCTCGAGATG GAGAGCGACG 
     3721 AGAGCGGCCT GCCCGCCATG GAGATCGAGT GCCGCATCAC CGGCACCCTG AACGGCGTGG 
     3781 AGTTCGAGCT GGTGGGCGGC GGAGAGGGCA CCCCCGAGCA GGGCCGCATG ACCAACAAGA 
     3841 TGAAGAGCAC CAAAGGCGCC CTGACCTTCA GCCCCTACCT GCTGAGCCAC GTGATGGGCT 
     3901 ACGGCTTCTA CCACTTCGGC ACCTACCCCA GCGGCTACGA GAACCCCTTC CTGCACGCCA 
     3961 TCAACAACGG CGGCTACACC AACACCCGCA TCGAGAAGTA CGAGGACGGC GGCGTGCTGC 
     4021 ACGTGAGCTT CAGCTACCGC TACGAGGCCG GCCGCGTGAT CGGCGACTTC AAGGTGATGG 
     4081 GCACCGGCTT CCCCGAGGAC AGCGTGATCT TCACCGACAA GATCATCCGC AGCAACGCCA 
     4141 CCGTGGAGCA CCTGCACCCC ATGGGCGATA ACGATCTGGA TGGCAGCTTC ACCCGCACCT 
     4201 TCAGCCTGCG CGACGGCGGC TACTACAGCT CCGTGGTGGA CAGCCACATG CACTTCAAGA 
     4261 GCGCCATCCA CCCCAGCATC CTGCAGAACG GGGGCCCCAT GTTCGCCTTC CGCCGCGTGG 
     4321 AGGAGGATCA CAGCAACACC GAGCTGGGCA TCGTGGAGTA CCAGCACGCC TTCAAGACCC 
     4381 CGGATGCAGA TGCCGGTGAA GAAAGAGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4441 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4501 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4561 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4621 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4681 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4741 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4801 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     4861 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     4921 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     4981 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5041 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC TTGGCTGTGG 
     5101 AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA 
     5161 AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC 
     5221 AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG 
     5281 CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT 
     5341 TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG AGCTATTCCA GAAGTAGTGA 
     5401 GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT 
     5461 TTCGGATCTG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG 
     5521 CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG 
     5581 ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT 
     5641 TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA 
     5701 TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG 
     5761 GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT 
     5821 GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT 
     5881 CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG 
     5941 ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA 
     6001 GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC 
     6061 CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC 
     6121 GACTGTGGCC GGCTGGGTGT GGCCGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT 
     6181 ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC 
     6241 GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA 
     6301 CTCTGGGGTT CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA GATTTCGATT 
     6361 CCACCGCCGC CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA 
     6421 TGATCCTCCA GCGCGGGGAT CTCATGCTGG AGTTCTTCGC CCACCCCAAC TTGTTTATTG 
     6481 CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT 
     6541 TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGTA 
     6601 TACCGTCGAC CTCTAGCTAG AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA 
     6661 ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT 
     6721 GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC 
     6781 AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG 
     6841 GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC 
     6901 GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG 
     6961 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA 
     7021 AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC 
     7081 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC 
     7141 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG 
     7201 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT 
     7261 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC 
     7321 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC 
     7381 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG 
     7441 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG 
     7501 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA 
     7561 CCACCGCTGG TAGCGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT 
     7621 CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC 
     7681 GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT 
     7741 AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC 
     7801 AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG 
     7861 CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG 
     7921 CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC 
     7981 CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA 
     8041 TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG 
     8101 TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT 
     8161 CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA 
     8221 GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG 
     8281 TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA 
     8341 CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT 
     8401 GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA 
     8461 TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT 
     8521 CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT 
     8581 CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA 
     8641 AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT 
     8701 GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC 
     8761 GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG 
     8821 GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT 
     8881 TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC 
     8941 GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG 
     9001 ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA 
     9061 CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC 
     9121 CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA 
     9181 AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//