LOCUS       RG226230                9194 bp    DNA     circular  SYN 
DEFINITION  VAV2 (tGFP-tagged) - Human vav 2 guanine nucleotide exchange factor (VAV2), transcript variant 1
ACCESSION   NM_001134398
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3675..4379 
                     /label="GFP"  
     misc_feature    4426..5011 
                     /label="PolyA signal"  
     misc_feature    7103..7748 
                     /label="ColE1"  
     misc_feature    5465..6259 
                     /label="Neo"  
     misc_feature    5096..5398 
                     /label="SV40 ori"  
     misc_feature    7763..8623 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3654..3660 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3654 
                     /label="VAV2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCAGTGG CGGCAGTGCG GCCGCTGGCT CATCGATTGC AAGGTCCTGC 
     1081 CGCCCAACCA CCGGGTGGTG TGGCCCTCGG CCGTGGTCTT CGACCTGGCG CAGGCGCTGC 
     1141 GCGACGGGGT CCTTCTGTGC CAGCTGCTGC ACAACCTCTC CCCCGGCTCC ATCGACCTCA 
     1201 AGGACATCAA CTTCCGGCCG CAGATGTCCC AGTTTCTGTG TTTGAAGAAC ATACGCACCT 
     1261 TCCTGAAAGT CTGCCACGAT AAATTTGGAT TAAGGAACAG CGAGCTGTTT GACCCCTTTG 
     1321 ACCTCTTCGA TGTGCGAGAC TTTGGAAAGG TCATCTCCGC GGTGTCGAGG CTCTCCCTGC 
     1381 ACAGCATCGC GCAGAACAAA GGGATCAGGC CTTTTCCCTC AGAGGAGACC ACAGAGAATG 
     1441 ACGATGACGT CTACCGCAGC CTGGAGGAGC TGGCCGACGA GCATGACCTG GGGGAGGACA 
     1501 TCTACGACTG CGTCCCGTGT GAGGATGGAG GGGACGACAT CTACGAGGAC ATCATCAAGG 
     1561 TGGAGGTGCA GCAGCCCATG ATTAGATACA TGCAGAAAAT GGGCATGACT GAAGATGACA 
     1621 AGAGGAACTG CTGCCTGCTG GAGATCCAGG AGACCGAGGC CAAGTACTAC CGCACCCTGG 
     1681 AGGACATTGA GAAGAACTAC ATGAGCCCCC TGCGGCTGGT GCTGAGCCCG GCGGACATGG 
     1741 CAGCTGTCTT CATTAACCTG GAGGACCTGA TCAAGGTGCA TCACAGCTTC CTGAGGGCCA 
     1801 TCGACGTGTC CGTGATGGTG GGGGGCAGCA CGCTGGCCAA GGTCTTCCTC GATTTCAAGG 
     1861 AAAGGCTTCT GATCTACGGG GAGTACTGCA GCCACATGGA GCACGCCCAG AACACACTGA 
     1921 ACCAGCTCCT GGCCAGCCGG GAGGACTTCA GGCAGAAAGT CGAGGAGTGC ACACTGAAGG 
     1981 TCCAGGATGG AAAATTTAAG CTGCAAGACC TGCTGGTGGT CCCCATGCAG AGGGTGCTCA 
     2041 AATACCACCT GCTCTTGAAG GAGCTTCTGA GCCATTCTGC GGAACGGCCT GAGAGGCAGC 
     2101 AGCTCAAAGA AGCACTGGAA GCCATGCAGG ACTTGGCGAT GTACATCAAT GAAGTTAAAC 
     2161 GGGACAAGGA GACCTTGAGG AAAATCAGCG AATTTCAGAG TTCTATAGAA AATTTGCAAG 
     2221 TGAAACTGGA GGAATTTGGA AGACCAAAGA TTGACGGGGA ACTGAAAGTC CGGTCCATAG 
     2281 TCAACCACAC CAAGCAGGAC AGGTACTTGT TCCTGTTTGA CAAGGTGGTC ATCGTCTGCA 
     2341 AGCGGAAGGG CTACAGCTAC GAGCTCAAGG AGATCATCGA GCTGCTGTTC CACAAGATGA 
     2401 CCGACGACCC CATGAACAAC AAGGACGTCA AGAAGTCTCA CGGGAAAATG TGGTCCTACG 
     2461 GCTTCTACCT AATTCACCTT CAAGGAAAGC AGGGCTTCCA GTTTTTCTGC AAAACAGAAG 
     2521 ATATGAAGAG GAAGTGGATG GAGCAGTTTG AGATGGCCAT GTCAAACATC AAGCCAGACA 
     2581 AAGCCAATGC CAACCACCAC AGTTTCCAGA TGTACACGTT TGACAAGACC ACCAACTGCA 
     2641 AAGCCTGCAA AATGTTCCTC AGGGGCACCT TCTACCAGGG ATACATGTGT ACCAAGTGTG 
     2701 GCGTCGGGGC ACACAAGGAG TGCCTGGAAG TGATACCTCC CTGCAAGTTC ACTTCTCCTG 
     2761 CAGATCTGGA CGCCTCCGGA GCGGGACCAG GTCCCAAGAT GGTGGCCATG CAGAATTACC 
     2821 ATGGCAACCC AGCCCCTCCC GGGAAGCCTG TGCTGACCTT CCAGACGGGC GACGTGCTTG 
     2881 AGCTGCTGAG GGGCGACCCT GAGTCTCCGT GGTGGGAGGG TCGTCTGGTA CAAACCAGGA 
     2941 AGTCAGGGTA TTTCCCCAGC TCATCTGTGA AGCCCTGCCC TGTGGATGGA AGGCCGCCCA 
     3001 TCAGCCGGCC GCCATCCCGG GAGATCGACT ACACTGCATA CCCCTGGTTT GCAGGTAACA 
     3061 TGGAGAGGCA GCAGACGGAC AACCTGCTCA AGTCCCACGC CAGCGGGACC TACCTGATCA 
     3121 GGGAGCGGCC TGCCGAGGCT GAGCGCTTTG CAATAAGCAT CAAGTTCAAT GATGAGGTGA 
     3181 AGCACATCAA GGTGGTGGAG AAGGACAACT GGATCCACAT CACAGAGGCC AAGAAATTCG 
     3241 ACAGCCTCCT GGAGTTGGTG GAGTACTACC AGTGCCACTC ACTGAAGGAG AGCTTCAAGC 
     3301 AGCTGGACAC CACACTCAAG TACCCCTACA AGTCCCGGGA ACGTTCGGCC TCCAGGGCCT 
     3361 CCAGCCGGTC CCCAGCTTCC TGTGCTTCCT ACAACTTTTC TTTTCTCAGT CCTCAGGGCC 
     3421 TCAGCTTTGC TTCTCAGGGC CCCTCCGCTC CCTTCTGGTC AGTGTTCACG CCCCGCGTCA 
     3481 TCGGCACAGC TGTGGCCAGG TATAACTTTG CCGCCCGAGA TATGAGGGAG CTTTCGCTGC 
     3541 GGGAGGGTGA CGTGGTGAGG ATCTACAGCC GCATCGGCGG AGACCAGGGC TGGTGGAAGG 
     3601 GCGAGACCAA CGGACGGATT GGCTGGTTTC CTTCAACGTA CGTAGAAGAG GAGGGCATCC 
     3661 AGACGCGTAC GCGGCCGCTC GAGATGGAGA GCGACGAGAG CGGCCTGCCC GCCATGGAGA 
     3721 TCGAGTGCCG CATCACCGGC ACCCTGAACG GCGTGGAGTT CGAGCTGGTG GGCGGCGGAG 
     3781 AGGGCACCCC CGAGCAGGGC CGCATGACCA ACAAGATGAA GAGCACCAAA GGCGCCCTGA 
     3841 CCTTCAGCCC CTACCTGCTG AGCCACGTGA TGGGCTACGG CTTCTACCAC TTCGGCACCT 
     3901 ACCCCAGCGG CTACGAGAAC CCCTTCCTGC ACGCCATCAA CAACGGCGGC TACACCAACA 
     3961 CCCGCATCGA GAAGTACGAG GACGGCGGCG TGCTGCACGT GAGCTTCAGC TACCGCTACG 
     4021 AGGCCGGCCG CGTGATCGGC GACTTCAAGG TGATGGGCAC CGGCTTCCCC GAGGACAGCG 
     4081 TGATCTTCAC CGACAAGATC ATCCGCAGCA ACGCCACCGT GGAGCACCTG CACCCCATGG 
     4141 GCGATAACGA TCTGGATGGC AGCTTCACCC GCACCTTCAG CCTGCGCGAC GGCGGCTACT 
     4201 ACAGCTCCGT GGTGGACAGC CACATGCACT TCAAGAGCGC CATCCACCCC AGCATCCTGC 
     4261 AGAACGGGGG CCCCATGTTC GCCTTCCGCC GCGTGGAGGA GGATCACAGC AACACCGAGC 
     4321 TGGGCATCGT GGAGTACCAG CACGCCTTCA AGACCCCGGA TGCAGATGCC GGTGAAGAAA 
     4381 GAGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4441 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4501 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4561 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4621 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4681 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4741 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4801 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4861 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     4921 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     4981 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5041 CCTGTTGAAT TGGGTACGCG GCCAGCTTGG CTGTGGAATG TGTGTCAGTT AGGGTGTGGA 
     5101 AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA 
     5161 ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC 
     5221 AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC 
     5281 AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG 
     5341 GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC 
     5401 TTTTGCAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA 
     5461 TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG 
     5521 GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC 
     5581 GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT 
     5641 GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT 
     5701 CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC 
     5761 GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC 
     5821 ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC 
     5881 CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG 
     5941 GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG 
     6001 GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT 
     6061 ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCC 
     6121 GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA 
     6181 TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC 
     6241 TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGTTCGAA ATGACCGACC 
     6301 AAGCGACGCC CAACCTGCCA TCACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT 
     6361 TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT CCTCCAGCGC GGGGATCTCA 
     6421 TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA 
     6481 GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT 
     6541 TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC GTCGACCTCT AGCTAGAGCT 
     6601 TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC 
     6661 ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC 
     6721 TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC 
     6781 TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG 
     6841 CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC 
     6901 ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT 
     6961 GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC 
     7021 ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA 
     7081 ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC 
     7141 CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG 
     7201 CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC 
     7261 TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC 
     7321 GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA 
     7381 GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT 
     7441 ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG 
     7501 GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTTTTTTTG 
     7561 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT 
     7621 CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT 
     7681 TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT 
     7741 AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA 
     7801 TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA 
     7861 CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC 
     7921 GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA 
     7981 GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG 
     8041 TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG 
     8101 TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG 
     8161 TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG 
     8221 TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC 
     8281 TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT 
     8341 TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA 
     8401 CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA 
     8461 AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA 
     8521 ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC 
     8581 AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC 
     8641 TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG 
     8701 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC 
     8761 CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA 
     8821 CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG 
     8881 CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT 
     8941 TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG 
     9001 GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA 
     9061 GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT 
     9121 TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT 
     9181 TTAACGCGAA TTTT
//