LOCUS       RG226198                9050 bp    DNA     circular  SYN 
DEFINITION  CDC27 (tGFP-tagged) - Human cell division cycle 27 homolog (S. cerevisiae) (CDC27), transcript variant 1
ACCESSION   NM_001114091
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3531..4235 
                     /label="GFP"  
     misc_feature    4282..4867 
                     /label="PolyA signal"  
     misc_feature    6959..7604 
                     /label="ColE1"  
     misc_feature    5321..6115 
                     /label="Neo"  
     misc_feature    4952..5254 
                     /label="SV40 ori"  
     misc_feature    7619..8479 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3510..3516 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3510 
                     /label="CDC27"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACGGTGCTG CAGGAACCCG TCCAGGCTGC TATATGGCAA GCACTAAACC 
     1081 ACTATGCTTA CCGAGATGCG GTTTTCCTCG CAGAACGCCT TTATGCAGAA GTACACTCAG 
     1141 AAGAAGCCTT GTTTTTACTG GCAACCTGTT ATTACCGCTC AGGAAAGGCA TATAAAGCAT 
     1201 ATAGACTCTT GAAAGGACAC AGTTGTACTA CACCGCAATG CAAATACCTG CTTGCAAAAT 
     1261 GTTGTGTTGA TCTCAGCAAG CTTGCAGAAG GGGAACAAAT CTTATCTGGT GGAGTGTTTA 
     1321 ATAAGCAGAA AAGCCATGAT GATATTGTTA CTGAGTTTGG TGATTCAGCT TGCTTTACTC 
     1381 TTTCATTGTT GGGACATGTA TATTGCAAGA CAGATCGGCT TGCCAAAGGA TCAGAATGTT 
     1441 ACCAAAAGAG CCTTAGTTTA AATCCTTTCC TCTGGTCTCC CTTTGAATCA TTATGTGAAA 
     1501 TAGGTGAAAA GCCAGATCCT GACCAAACAT TTAAATTCAC ATCTTTACAG AACTTTAGCA 
     1561 ACTGTCTGCC CAACTCTTGC ACAACACAAG TACCTAATCA TAGTTTATCT CACAGACAGC 
     1621 CTGAGACAGT TCTTACGGAA ACACCCCAGG ACACAATTGA ATTAAACAGA TTGAATTTAG 
     1681 AATCTTCCAA TTCAAAGTAC TCCTTGAATA CAGATTCCTC AGTGTCTTAT ATTGATTCAG 
     1741 CTGTAATTTC ACCTGATACT GTCCCACTGG GAACAGGAAC TTCCATATTA TCTAAACAGG 
     1801 TTCAAAATAA ACCAAAAACT GGTCGAAGTT TATTAGGAGG ACCAGCAGCT CTTAGTCCAT 
     1861 TAACCCCAAG TTTTGGGATT TTGCCATTAG AAACCCCAAG TCCTGGAGAT GGATCCTATT 
     1921 TACAAAACTA CACTAATACA CCTCCTGTAA TTGATGTGCC ATCCACCGGA GCCCCTTCAA 
     1981 AAAAGACTTT TCGTGTTTTA CAGTCTGTTG CCAGAATCGG CCAAACTGGA ACAAAGTCTG 
     2041 TCTTCTCACA GAGTGGAAAT AGCCGAGAGG TAACTCCAAT TCTTGCACAA ACACAAAGTT 
     2101 CTGGTCCACA AACAAGTACA ACACCTCAGG TATTGAGCCC CACTATTACA TCTCCCCCAA 
     2161 ACGCACTGCC TCGAAGAAGT TCACGACTCT TTACTAGTGA CAGCTCCACA ACCAAGGAGA 
     2221 ATAGCAAAAA ATTAAAAATG AAGTTTCCAC CTAAAATCCC AAACAGAAAA ACAAAAAGTA 
     2281 AAACTAATAA AGGAGGAATA ACTCAACCTA ACATAAATGA TAGCCTGGAA ATTACAAAAT 
     2341 TGGACTCTTC CATCATTTCA GAAGGGAAAA TATCCACAAT CACACCTCAG ATTCAGGCCT 
     2401 TTAATCTACA AAAAGCAGCA GCAGAAGGTT TGATGAGCCT TCTTCGTGAA ATGGGGAAAG 
     2461 GTTATTTAGC TTTGTGTTCA TACAACTGCA AAGAAGCTAT AAATATTTTG AGCCATCTAC 
     2521 CTTCTCACCA CTACAATACT GGTTGGGTAC TGTGCCAAAT TGGAAGGGCC TATTTTGAAC 
     2581 TTTCAGAGTA CATGCAAGCT GAAAGAATAT TCTCAGAGGT TAGAAGGATT GAGAATTATA 
     2641 GAGTTGAAGG CATGGAGATC TACTCTACAA CACTTTGGCA TCTTCAAAAA GATGTTGCTC 
     2701 TTTCAGTTCT GTCAAAAGAC TTAACAGACA TGGATAAAAA TTCGCCAGAG GCCTGGTGTG 
     2761 CTGCAGGGAA CTGTTTCAGT CTGCAACGGG AACATGATAT TGCAATTAAA TTCTTCCAGA 
     2821 GAGCTATCCA AGTTGATCCA AATTACGCTT ATGCCTATAC TCTATTAGGG CATGAGTTTG 
     2881 TCTTAACTGA AGAATTGGAC AAAGCATTAG CTTGTTTTCG AAATGCTATC AGAGTCAATC 
     2941 CTAGACATTA TAATGCATGG TATGGTTTAG GAATGATTTA TTACAAGCAA GAAAAATTCA 
     3001 GCCTTGCAGA AATGCATTTC CAAAAAGCGC TTGATATCAA CCCTCAAAGT TCAGTTTTAC 
     3061 TTTGCCACAT TGGAGTAGTT CAACATGCAC TGAAAAAATC AGAGAAGGCT TTGGATACCC 
     3121 TAAACAAAGC CATTGTCATT GATCCCAAGA ACCCTCTATG CAAATTTCAC AGAGCCTCAG 
     3181 TTTTATTTGC AAATGAAAAA TATAAGTCTG CTTTACAAGA ACTTGAAGAA TTGAAACAAA 
     3241 TTGTTCCCAA AGAATCCCTC GTTTACTTCT TAATAGGAAA GGTTTACAAG AAGTTAGGTC 
     3301 AAACGCACCT CGCCCTGATG AATTTCTCTT GGGCTATGGA TTTAGATCCT AAAGGAGCCA 
     3361 ATAACCAGAT TAAAGAGGCA ATTGATAAGC GTTATCTTCC AGATGATGAG GAGCCAATAA 
     3421 CCCAAGAAGA ACAGATCATG GGAACAGATG AATCCCAGGA GAGCAGCATG ACAGATGCGG 
     3481 ATGACACACA ACTTCATGCA GCTGAAAGTG ATGAATTTAC GCGTACGCGG CCGCTCGAGA 
     3541 TGGAGAGCGA CGAGAGCGGC CTGCCCGCCA TGGAGATCGA GTGCCGCATC ACCGGCACCC 
     3601 TGAACGGCGT GGAGTTCGAG CTGGTGGGCG GCGGAGAGGG CACCCCCGAG CAGGGCCGCA 
     3661 TGACCAACAA GATGAAGAGC ACCAAAGGCG CCCTGACCTT CAGCCCCTAC CTGCTGAGCC 
     3721 ACGTGATGGG CTACGGCTTC TACCACTTCG GCACCTACCC CAGCGGCTAC GAGAACCCCT 
     3781 TCCTGCACGC CATCAACAAC GGCGGCTACA CCAACACCCG CATCGAGAAG TACGAGGACG 
     3841 GCGGCGTGCT GCACGTGAGC TTCAGCTACC GCTACGAGGC CGGCCGCGTG ATCGGCGACT 
     3901 TCAAGGTGAT GGGCACCGGC TTCCCCGAGG ACAGCGTGAT CTTCACCGAC AAGATCATCC 
     3961 GCAGCAACGC CACCGTGGAG CACCTGCACC CCATGGGCGA TAACGATCTG GATGGCAGCT 
     4021 TCACCCGCAC CTTCAGCCTG CGCGACGGCG GCTACTACAG CTCCGTGGTG GACAGCCACA 
     4081 TGCACTTCAA GAGCGCCATC CACCCCAGCA TCCTGCAGAA CGGGGGCCCC ATGTTCGCCT 
     4141 TCCGCCGCGT GGAGGAGGAT CACAGCAACA CCGAGCTGGG CATCGTGGAG TACCAGCACG 
     4201 CCTTCAAGAC CCCGGATGCA GATGCCGGTG AAGAAAGAGT TTAAACGGCC GGCCGCGGTC 
     4261 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4321 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4381 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4441 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4501 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4561 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4621 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     4681 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     4741 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     4801 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     4861 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     4921 GCTTGGCTGT GGAATGTGTG TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC 
     4981 AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC 
     5041 TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG 
     5101 CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT 
     5161 GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCGGCCTC TGAGCTATTC 
     5221 CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT 
     5281 TGTATATCCA TTTTCGGATC TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA 
     5341 CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC 
     5401 TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG 
     5461 CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG 
     5521 GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT 
     5581 GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG 
     5641 TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG 
     5701 CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA 
     5761 GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG 
     5821 GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT 
     5881 CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT 
     5941 TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCCGACC GCTATCAGGA CATAGCGTTG 
     6001 GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT 
     6061 TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC 
     6121 TTCTGAGCGG GACTCTGGGG TTCGAAATGA CCGACCAAGC GACGCCCAAC CTGCCATCAC 
     6181 GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTCCGGG 
     6241 ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT GGAGTTCTTC GCCCACCCCA 
     6301 ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA 
     6361 ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT 
     6421 ATCATGTCTG TATACCGTCG ACCTCTAGCT AGAGCTTGGC GTAATCATGG TCATAGCTGT 
     6481 TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA 
     6541 AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC 
     6601 TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG 
     6661 CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC 
     6721 GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT 
     6781 CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA 
     6841 GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC 
     6901 ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC 
     6961 AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG 
     7021 GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA 
     7081 GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG 
     7141 TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC 
     7201 ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG 
     7261 GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT 
     7321 TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT 
     7381 CCGGCAAACA AACCACCGCT GGTAGCGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA 
     7441 GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA 
     7501 ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA 
     7561 TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT 
     7621 CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT 
     7681 CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT 
     7741 CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG 
     7801 CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT 
     7861 CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT 
     7921 TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG 
     7981 CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA 
     8041 AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT 
     8101 TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT 
     8161 GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC 
     8221 CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA 
     8281 AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT 
     8341 TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT 
     8401 TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA 
     8461 GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT 
     8521 ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA 
     8581 TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT 
     8641 TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG 
     8701 CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC 
     8761 AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC 
     8821 CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT 
     8881 TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA 
     8941 CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG 
     9001 CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//