LOCUS       RG226161                8891 bp    DNA     circular  SYN 
DEFINITION  PLCD1 (tGFP-tagged) - Human phospholipase C, delta 1 (PLCD1), transcript variant 1
ACCESSION   NM_001130964
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3372..4076 
                     /label="GFP"  
     misc_feature    4123..4708 
                     /label="PolyA signal"  
     misc_feature    6800..7445 
                     /label="ColE1"  
     misc_feature    5162..5956 
                     /label="Neo"  
     misc_feature    4793..5095 
                     /label="SV40 ori"  
     misc_feature    7460..8320 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3351..3357 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3351 
                     /label="PLCD1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGTGCCTG GGGATCCGGA GCCGGAGCCG CTCCAGGGAG CTCTACCTGC 
     1081 AGGAGCGGAG CCTTAAGGTG GCGGCGCTCA ATGGACGGAG GCTGGGCCTA CAGGATGATG 
     1141 AGGATCTACA GGCGCTGCTG AAGGGCAGCC AGCTCCTGAA GGTGAAGTCC AGCTCATGGA 
     1201 GGAGAGAGCG CTTCTACAAG TTGCAGGAGG ACTGCAAGAC CATCTGGCAG GAGTCCCGCA 
     1261 AGGTCATGCG GACCCCGGAG TCCCAGCTGT TCTCCATCGA GGACATTCAG GAGGTGCGAA 
     1321 TGGGGCACCG CACGGAGGGT CTGGAGAAGT TCGCCCGTGA TGTGCCCGAG GACCGCTGCT 
     1381 TCTCCATTGT CTTCAAGGAC CAGCGCAATA CACTAGACCT CATCGCCCCA TCGCCAGCTG 
     1441 ATGCCCAGCA CTGGGTGCTG GGGCTGCACA AGATCATCCA CCACTCAGGC TCCATGGACC 
     1501 AGCGTCAGAA GCTACAGCAC TGGATTCACT CCTGCTTGCG AAAAGCTGAC AAAAACAAGG 
     1561 ACAACAAGAT GAGCTTCAAG GAGCTGCAGA ACTTCCTGAA GGAGCTCAAC ATCCAGGTGG 
     1621 ACGACAGCTA TGCCCGGAAG ATCTTCAGGG AGTGTGACCA CTCCCAGACA GACTCCCTGG 
     1681 AGGACGAGGA GATTGAGGCC TTCTACAAGA TGCTGACCCA GCGGGTGGAG ATCGACCGCA 
     1741 CCTTCGCCGA GGCCGCGGGC TCAGGGGAGA CTCTGTCGGT GGATCAGTTA GTGACGTTCC 
     1801 TGCAGCACCA GCAGCGGGAG GAGGCGGCAG GGCCTGCGCT GGCCCTCTCC CTCATTGAGC 
     1861 GCTACGAGCC CAGCGAGACT GCCAAGGCGC AGCGGCAGAT GACCAAGGAC GGCTTCCTCA 
     1921 TGTACTTACT GTCGGCTGAC GGCAGCGCCT TCAGCCTGGC ACACCGCCGT GTCTACCAGG 
     1981 ACATGGGCCA GCCACTTAGC CACTACCTGG TGTCCTCTTC ACACAACACC TACCTGCTGG 
     2041 AGGACCAGCT AGCCGGGCCC AGCAGCACTG AAGCCTACAT CCGGGCACTG TGCAAAGGCT 
     2101 GCCGATGCCT GGAGCTTGAC TGCTGGGACG GGCCCAACCA GGAACCAATC ATCTACCACG 
     2161 GCTATACTTT CACTTCCAAG ATCCTCTTCT GCGATGTGCT CAGGGCCATC CGGGACTATG 
     2221 CCTTCAAGGC GTCCCCCTAC CCTGTCATCC TATCCCTGGA GAACCACTGC ACACTGGAGC 
     2281 AGCAGCGCGT GATGGCGCGG CACCTGCATG CCATCCTGGG CCCCATGCTG TTGAACCGAC 
     2341 CACTGGATGG GGTCACCAAC AGCCTGCCCT CCCCTGAGCA ACTGAAGGGG AAGATCCTGC 
     2401 TGAAGGGGAA GAAGCTCGGG GGGCTCCTGC CCCCTGGAGG GGAGGGTGGC CCTGAGGCCA 
     2461 CTGTGGTGTC AGACGAAGAC GAGGCTGCTG AGATGGAGGA TGAGGCAGTG AGGAGCCGTG 
     2521 TGCAGCACAA GCCCAAGGAG GACAAGCTCA GGCTAGCACA GGAGCTCTCT GACATGGTCA 
     2581 TTTACTGCAA GAGTGTCCAC TTTGGGGGCT TCTCCAGTCC TGGCACCCCT GGACAGGCCT 
     2641 TCTACGAGAT GGCGTCCTTC TCTGAGAACC GTGCCCTTCG ACTGCTCCAA GAATCAGGAA 
     2701 ACGGCTTTGT CCGCCACAAC GTGGGGCACC TGAGCAGAAT CTACCCGGCT GGATGGAGAA 
     2761 CAGACTCCTC CAACTACAGC CCCGTGGAGA TGTGGAATGG GGGCTGCCAG ATCGTGGCCC 
     2821 TGAATTTCCA GACACCTGGG CCAGAGATGG ACGTGTACCA GGGCCGCTTC CAGGACAACG 
     2881 GGGCCTGTGG GTACGTGCTG AAGCCCGCCT TCCTGCGAGA CCCCAACGGC ACCTTTAACC 
     2941 CCCGCGCCCT GGCTCAGGGG CCCTGGTGGG CACGGAAGCG GCTCAACATC AGGGTCATTT 
     3001 CGGGGCAGCA GCTGCCAAAA GTCAACAAGA ATAAGAATTC AATTGTGGAC CCCAAAGTGA 
     3061 CAGTGGAGAT CCATGGCGTG AGCCGGGACG TGGCCAGCCG CCAGACTGCT GTCATCACCA 
     3121 ACAATGGTTT CAACCCATGG TGGGACACGG AGTTTGCGTT TGAGGTAGTT GTGCCTGACC 
     3181 TTGCCCTCAT CCGCTTCTTG GTGGAAGATT ATGATGCCTC CTCCAAGAAT GACTTCATTG 
     3241 GCCAGAGTAC CATCCCCTTG AACAGCCTCA AGCAAGGATA CCGCCATGTC CACCTCATGT 
     3301 CTAAGAACGG GGACCAGCAT CCATCAGCCA CCCTCTTTGT GAAGATCTCC CTCCAGGACA 
     3361 CGCGTACGCG GCCGCTCGAG ATGGAGAGCG ACGAGAGCGG CCTGCCCGCC ATGGAGATCG 
     3421 AGTGCCGCAT CACCGGCACC CTGAACGGCG TGGAGTTCGA GCTGGTGGGC GGCGGAGAGG 
     3481 GCACCCCCGA GCAGGGCCGC ATGACCAACA AGATGAAGAG CACCAAAGGC GCCCTGACCT 
     3541 TCAGCCCCTA CCTGCTGAGC CACGTGATGG GCTACGGCTT CTACCACTTC GGCACCTACC 
     3601 CCAGCGGCTA CGAGAACCCC TTCCTGCACG CCATCAACAA CGGCGGCTAC ACCAACACCC 
     3661 GCATCGAGAA GTACGAGGAC GGCGGCGTGC TGCACGTGAG CTTCAGCTAC CGCTACGAGG 
     3721 CCGGCCGCGT GATCGGCGAC TTCAAGGTGA TGGGCACCGG CTTCCCCGAG GACAGCGTGA 
     3781 TCTTCACCGA CAAGATCATC CGCAGCAACG CCACCGTGGA GCACCTGCAC CCCATGGGCG 
     3841 ATAACGATCT GGATGGCAGC TTCACCCGCA CCTTCAGCCT GCGCGACGGC GGCTACTACA 
     3901 GCTCCGTGGT GGACAGCCAC ATGCACTTCA AGAGCGCCAT CCACCCCAGC ATCCTGCAGA 
     3961 ACGGGGGCCC CATGTTCGCC TTCCGCCGCG TGGAGGAGGA TCACAGCAAC ACCGAGCTGG 
     4021 GCATCGTGGA GTACCAGCAC GCCTTCAAGA CCCCGGATGC AGATGCCGGT GAAGAAAGAG 
     4081 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4141 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4201 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4261 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4321 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4381 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4441 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     4501 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     4561 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     4621 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     4681 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     4741 GTTGAATTGG GTACGCGGCC AGCTTGGCTG TGGAATGTGT GTCAGTTAGG GTGTGGAAAG 
     4801 TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC 
     4861 AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT 
     4921 TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT 
     4981 TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC 
     5041 GCCTCGGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT 
     5101 TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAAGA GACAGGATGA 
     5161 GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG 
     5221 GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG 
     5281 TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC 
     5341 CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT 
     5401 TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA 
     5461 GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG 
     5521 GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA 
     5581 GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT 
     5641 GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG 
     5701 CGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC 
     5761 ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCCGAC 
     5821 CGCTATCAGG ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG 
     5881 GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC 
     5941 TATCGCCTTC TTGACGAGTT CTTCTGAGCG GGACTCTGGG GTTCGAAATG ACCGACCAAG 
     6001 CGACGCCCAA CCTGCCATCA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG 
     6061 GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC 
     6121 TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA 
     6181 ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT 
     6241 CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC GACCTCTAGC TAGAGCTTGG 
     6301 CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA 
     6361 ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA 
     6421 CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC 
     6481 ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT 
     6541 CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT 
     6601 CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG 
     6661 CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA 
     6721 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 
     6781 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 
     6841 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 
     6901 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 
     6961 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 
     7021 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 
     7081 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 
     7141 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 
     7201 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT TTTTTTGTTT 
     7261 GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA 
     7321 CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT 
     7381 CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA 
     7441 GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT 
     7501 CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA 
     7561 CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT 
     7621 CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG 
     7681 GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA 
     7741 GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT 
     7801 CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA 
     7861 CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA 
     7921 GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA 
     7981 CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT 
     8041 GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG 
     8101 CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC 
     8161 TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT 
     8221 GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA 
     8281 ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT 
     8341 TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT 
     8401 GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG 
     8461 ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG 
     8521 CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA 
     8581 CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA 
     8641 GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC 
     8701 CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG 
     8761 GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT 
     8821 AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA 
     8881 ACGCGAATTT T
//