LOCUS       RG224831                9338 bp    DNA     circular  SYN 
DEFINITION  AASS (tGFP-tagged) - Human aminoadipate-semialdehyde synthase (AASS), nuclear gene encoding mitochondrial protein
ACCESSION   NM_005763
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3819..4523 
                     /label="GFP"  
     misc_feature    4570..5155 
                     /label="PolyA signal"  
     misc_feature    7247..7892 
                     /label="ColE1"  
     misc_feature    5609..6403 
                     /label="Neo"  
     misc_feature    5240..5542 
                     /label="SV40 ori"  
     misc_feature    7907..8767 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3798..3804 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3798 
                     /label="AASS"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTGCAAGTA CATAGGACTG GACTGGGCAG GCTGGGGGTC AGCCTCTCCA 
     1081 AGGGTCTTCA CCACAAAGCT GTGTTGGCCG TCCGGAGGGA GGATGTGAAC GCCTGGGAGA 
     1141 GAAGGGCCCC GCTAGCTCCC AAGCACATCA AAGGCATCAC CAATCTGGGA TACAAGGTCT 
     1201 TGATACAGCC TTCGAATCGG CGGGCCATTC ATGATAAGGA CTATGTCAAA GCTGGTGGCA 
     1261 TTCTTCAGGA GGATATTTCT GAAGCTTGTC TAATTTTAGG AGTTAAAAGA CCTCCAGAGG 
     1321 AAAAATTAAT GTCCAGGAAG ACTTATGCAT TTTTCTCCCA CACAATAAAA GCTCAGGAGG 
     1381 CCAATATGGG CTTGTTGGAT GAGATTCTAA AACAGGAAAT TCGCCTTATT GATTATGAGA 
     1441 AAATGGTGGA TCATAGAGGA GTACGGGTAG TGGCATTTGG ACAGTGGGCT GGTGTGGCAG 
     1501 GAATGATCAA CATTTTACAT GGAATGGGTT TAAGGCTCCT TGCTTTGGGA CATCACACAC 
     1561 CTTTTATGCA CATTGGCATG GCTCATAACT ACAGGAATAG CAGTCAGGCT GTGCAAGCTG 
     1621 TCCGTGATGC TGGCTATGAA ATATCTTTGG GTTTGATGCC TAAGTCAATA GGACCCTTAA 
     1681 CATTTGTGTT CACAGGAACT GGTAATGTTT CTAAGGGAGC CCAAGCAATC TTTAATGAGC 
     1741 TACCTTGTGA ATATGTGGAG CCCCATGAAT TAAAAGAAGT TTCCCAAACT GGAGACCTCA 
     1801 GAAAAGTGTA TGGGACGGTG TTAAGTCGTC ATCATCATCT TGTCAGGAAA ACAGATGCTG 
     1861 TGTATGATCC TGCAGAGTAT GACAAACATC CGGAGCGCTA CATAAGTCGT TTTAATACTG 
     1921 ATATTGCACC CTATACAACT TGCTTAATTA ATGGAATCTA CTGGGAACAA AACACTCCTC 
     1981 GCCTCCTAAC CCGCCAAGAT GCTCAGAGTC TCCTGGCTCC GGGCAAGTTC TCACCTGCTG 
     2041 GTGTGGAAGG CTGCCCTGCA TTACCACACA AACTCGTGGC AATATGTGAC ATTTCAGCTG 
     2101 ACACAGGAGG GTCTATAGAG TTTATGACTG AGTGTACAAC AATAGAGCAT CCCTTTTGCA 
     2161 TGTATGATGC AGACCAGCAT ATTATTCATG ACAGTGTTGA AGGCTCGGGG ATCCTGATGT 
     2221 GTTCCATTGA CAATTTGCCG GCACAGCTCC CAATTGAAGC TACAGAATGC TTTGGAGACA 
     2281 TGCTTTACCC TTATGTTGAA GAAATGATAT TATCAGACGC GACACAGCCT CTTGAAAGTC 
     2341 AGAATTTTTC TCCTGTGGTG AGAGATGCAG TGATTACATC CAACGGTACA TTACCTGATA 
     2401 AATATAAATA TATCCAGACA CTCCGGGAGA GCAGGGAACG TGCTCAGTCA CTTTCAATGG 
     2461 GCACCAGGAG AAAGGTTTTG GTTCTTGGAT CTGGCTACAT ATCTGAGCCT GTATTAGAAT 
     2521 ATTTATCAAG AGATGGCAAT ATAGAAATAA CAGTAGGATC TGACATGAAG AATCAAATTG 
     2581 AACAGTTAGG CAAGAAATAT AATATTAATC CTGTTAGCAT GGACATTTGT AAACAAGAAG 
     2641 AGAAGCTGGG CTTCTTGGTG GCAAAACAGG ATCTTGTCAT CAGCTTGTTG CCTTATGTAT 
     2701 TGCACCCTCT TGTGGCCAAG GCCTGCATCA CAAACAAAGT TAACATGGTC ACTGCAAGCT 
     2761 ACATCACACC AGCACTAAAA GAATTGGAAA AGAGTGTGGA AGATGCTGGC ATCACAATCA 
     2821 TTGGTGAATT GGGATTGGAC CCTGGTCTGG ATCACATGTT AGCAATGGAA ACAATAGATA 
     2881 AAGCCAAGGA AGTGGGAGCC ACGATTGAAT CATATATTTC CTACTGTGGT GGGCTTCCAG 
     2941 CCCCTGAACA TTCAAACAAT CCATTGAGAT ATAAATTTAG CTGGAGTCCA GTGGGAGTTT 
     3001 TGATGAATGT AATGCAGTCT GCCACCTATC TGCTCGATGG AAAGGTTGTG AATGTTGCAG 
     3061 GAGGCATCTC CTTTCTTGAT GCCGTTACGT CCATGGATTT TTTTCCAGGA TTAAATTTGG 
     3121 AAGGCTATCC TAACAGAGAC AGTACGAAAT ATGCTGAGAT TTATGGCATT TCTTCTGCTC 
     3181 ACACTTTGTT GCGGGGGACA CTGAGATATA AGGGATATAT GAAAGCTTTG AATGGATTTG 
     3241 TAAAATTAGG TCTTATAAAC AGAGAAGCGC TTCCTGCCTT TAGACCTGAG GCCAACCCTC 
     3301 TCACCTGGAA ACAACTCCTC TGTGACCTAG TTGGGATTTC ACCCTCCTCT GAGCATGATG 
     3361 TGTTGAAGGA AGCTGTTCTT AAGAAACTAG GAGGAGACAA TACCCAGTTG GAGGCTGCTG 
     3421 AATGGTTGGG CTTACTTGGG GATGAACAAG TTCCTCAGGC AGAGTCCATT CTGGATGCCC 
     3481 TCTCCAAGCA TTTGGTCATG AAGCTTTCCT ATGGTCCTGA AGAAAAAGAT ATGATTGTGA 
     3541 TGAGAGACAG CTTTGGAATC AGACATCCTT CTGGACATTT AGAACATAAA ACGATTGATC 
     3601 TTGTGGCTTA TGGGGACATC AATGGCTTTT CAGCCATGGC TAAAACCGTG GGGTTACCCA 
     3661 CCGCCATGGC AGCCAAAATG TTGCTTGATG GTGAAATTGG AGCCAAAGGC CTAATGGGGC 
     3721 CCTTTTCAAA GGAGATCTAT GGACCAATAT TGGAGCGAAT TAAAGCAGAA GGCATTATAT 
     3781 ATACTACACA GAGTACAATT AAACCAACGC GTACGCGGCC GCTCGAGATG GAGAGCGACG 
     3841 AGAGCGGCCT GCCCGCCATG GAGATCGAGT GCCGCATCAC CGGCACCCTG AACGGCGTGG 
     3901 AGTTCGAGCT GGTGGGCGGC GGAGAGGGCA CCCCCGAGCA GGGCCGCATG ACCAACAAGA 
     3961 TGAAGAGCAC CAAAGGCGCC CTGACCTTCA GCCCCTACCT GCTGAGCCAC GTGATGGGCT 
     4021 ACGGCTTCTA CCACTTCGGC ACCTACCCCA GCGGCTACGA GAACCCCTTC CTGCACGCCA 
     4081 TCAACAACGG CGGCTACACC AACACCCGCA TCGAGAAGTA CGAGGACGGC GGCGTGCTGC 
     4141 ACGTGAGCTT CAGCTACCGC TACGAGGCCG GCCGCGTGAT CGGCGACTTC AAGGTGATGG 
     4201 GCACCGGCTT CCCCGAGGAC AGCGTGATCT TCACCGACAA GATCATCCGC AGCAACGCCA 
     4261 CCGTGGAGCA CCTGCACCCC ATGGGCGATA ACGATCTGGA TGGCAGCTTC ACCCGCACCT 
     4321 TCAGCCTGCG CGACGGCGGC TACTACAGCT CCGTGGTGGA CAGCCACATG CACTTCAAGA 
     4381 GCGCCATCCA CCCCAGCATC CTGCAGAACG GGGGCCCCAT GTTCGCCTTC CGCCGCGTGG 
     4441 AGGAGGATCA CAGCAACACC GAGCTGGGCA TCGTGGAGTA CCAGCACGCC TTCAAGACCC 
     4501 CGGATGCAGA TGCCGGTGAA GAAAGAGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4561 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4621 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4681 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4741 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4801 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4861 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4921 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     4981 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5041 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5101 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5161 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC TTGGCTGTGG 
     5221 AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA 
     5281 AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC 
     5341 AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG 
     5401 CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT 
     5461 TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG AGCTATTCCA GAAGTAGTGA 
     5521 GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT 
     5581 TTCGGATCTG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG 
     5641 CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG 
     5701 ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT 
     5761 TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA 
     5821 TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG 
     5881 GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT 
     5941 GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT 
     6001 CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG 
     6061 ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA 
     6121 GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC 
     6181 CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC 
     6241 GACTGTGGCC GGCTGGGTGT GGCCGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT 
     6301 ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC 
     6361 GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA 
     6421 CTCTGGGGTT CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA GATTTCGATT 
     6481 CCACCGCCGC CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA 
     6541 TGATCCTCCA GCGCGGGGAT CTCATGCTGG AGTTCTTCGC CCACCCCAAC TTGTTTATTG 
     6601 CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT 
     6661 TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGTA 
     6721 TACCGTCGAC CTCTAGCTAG AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA 
     6781 ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT 
     6841 GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC 
     6901 AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG 
     6961 GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC 
     7021 GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG 
     7081 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA 
     7141 AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC 
     7201 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC 
     7261 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG 
     7321 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT 
     7381 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC 
     7441 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC 
     7501 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG 
     7561 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG 
     7621 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA 
     7681 CCACCGCTGG TAGCGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT 
     7741 CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC 
     7801 GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT 
     7861 AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC 
     7921 AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG 
     7981 CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG 
     8041 CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC 
     8101 CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA 
     8161 TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG 
     8221 TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT 
     8281 CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA 
     8341 GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG 
     8401 TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA 
     8461 CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT 
     8521 GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA 
     8581 TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT 
     8641 CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT 
     8701 CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA 
     8761 AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT 
     8821 GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC 
     8881 GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG 
     8941 GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT 
     9001 TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC 
     9061 GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG 
     9121 ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA 
     9181 CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC 
     9241 CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA 
     9301 AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//