LOCUS       RG224695                9500 bp    DNA     circular  SYN 
DEFINITION  GRIK5 (tGFP-tagged) - Human glutamate receptor, ionotropic, kainate 5 (GRIK5)
ACCESSION   NM_002088
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3981..4685 
                     /label="GFP"  
     misc_feature    4732..5317 
                     /label="PolyA signal"  
     misc_feature    7409..8054 
                     /label="ColE1"  
     misc_feature    5771..6565 
                     /label="Neo"  
     misc_feature    5402..5704 
                     /label="SV40 ori"  
     misc_feature    8069..8929 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3960..3966 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3960 
                     /label="GRIK5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGGCTGAG CTGCTGCTGC TGCTGATTGT TGCCTTCGCC AGCCCCAGCT 
     1081 GCCAGGTGCT CTCATCACTG CGCATGGCTG CAATCCTGGA TGATCAGACA GTGTGTGGCC 
     1141 GCGGTGAGCG TCTGGCCTTG GCCTTGGCCC GGGAGCAGAT CAACGGGATC ATCGAGGTCC 
     1201 CAGCCAAGGC CCGAGTGGAA GTAGACATCT TTGAGCTGCA GCGGGACAGC CAGTACGAGA 
     1261 CCACGGACAC CATGTGTCAG ATCTTACCCA AAGGGGTTGT GTCTGTCCTT GGGCCCTCCT 
     1321 CTAGCCCAGC ATCTGCCTCC ACCGTGAGCC ATATCTGTGG AGAGAAGGAG ATCCCCCACA 
     1381 TCAAGGTGGG TCCCGAGGAG ACACCCCGCC TTCAGTACCT TCGCTTCGCG TCTGTCAGCC 
     1441 TGTACCCCAG TAACGAGGAC GTCAGCTTGG CGGTCTCCCG AATCCTCAAG TCCTTCAACT 
     1501 ACCCCTCGGC CAGCCTCATC TGCGCCAAGG CTGAGTGCCT GCTGCGATTG GAGGAACTGG 
     1561 TGCGTGGCTT CCTCATCTCC AAGGAGACGC TGTCAGTGAG GATGTTGGAC GACAGCCGGG 
     1621 ACCCCACACC ACTGCTCAAG GAGATCCGTG ATGACAAGGT GTCCACCATC ATCATCGACG 
     1681 CCAACGCCTC CATCTCCCAC CTCATCCTCC GTAAGGCCTC GGAACTGGGA ATGACCTCAG 
     1741 CGTTTTACAA GTACATCCTC ACCACCATGG ACTTCCCCAT CCTGCATCTG GACGGTATTG 
     1801 TGGAGGACTC CTCCAACATC CTGGGCTTCT CCATGTTCAA CACGTCCCAC CCCTTCTACC 
     1861 CTGAGTTTGT CCGCAGCCTC AACATGTCCT GGAGGGAGAA CTGTGAAGCC AGCACCTACC 
     1921 TGGGCCCTGC GCTGTCAGCC GCCCTGATGT TTGACGCCGT GCACGTGGTG GTGAGCGCTG 
     1981 TCCGAGAGCT GAACCGCAGC CAGGAGATCG GTGTGAAGCC TCTGGCCTGT ACATCGGCCA 
     2041 ACATTTGGCC CCACGGGACC AGCCTCATGA ACTACCTGCG CATGGTAGAG TATGATGGGC 
     2101 TGACCGGGCG GGTCGAGTTC AACAGCAAAG GGCAGAGAAC CAACTACACC CTGCGCATCC 
     2161 TAGAAAAGTC CCGGCAGGGC CACCGTGAGA TTGGGGTGTG GTACTCTAAC CGCACCCTGG 
     2221 CCATGAATGC CACCACCCTG GACATCAACC TGTCGCAGAC ACTGGCCAAC AAGACCCTGG 
     2281 TGGTCACAAC CATCCTGGAG AACCCATACG TCATGCGCCG GCCCAACTTC CAGGCCCTGT 
     2341 CGGGGAACGA ACGCTTCGAG GGCTTCTGCG TGGACATGCT GCGGGAGCTG GCCGAGCTGC 
     2401 TGCGCTTCCG CTACCGCCTG CGGTTGGTGG AGGATGGGCT GTACGGGGCG CCCGAGCCCA 
     2461 ACGGCTCCTG GACGGGCATG GTTGGCGAGC TCATCAACCG GAAGGCAGAC CTGGCTGTGG 
     2521 CCGCCTTCAC CATCACAGCT GAGCGGGAGA AGGTCATCGA CTTTTCCAAG CCCTTTATGA 
     2581 CCCTGGGGAT CAGCATCCTC TACCGAGTGC ACATGGGCCG CAAGCCTGGC TACTTCTCCT 
     2641 TCCTGGACCC CTTCTCCCCT GCTGTGTGGC TCTTCATGCT TCTTGCCTAC CTGGCTGTCA 
     2701 GCTGCGTCCT GTTTCTGGCT GCCAGGCTGA GCCCCTATGA GTGGTATAAC CCACACCCAT 
     2761 GCCTGCGGGC ACGCCCCCAC ATCCTGGAGA ACCAGTACAC GCTGGGCAAC AGCCTGTGGT 
     2821 TTCCCGTGGG GGGCTTCATG CAGCAGGGCT CGGAGATCAT GCCCCGGGCG CTGTCCACGC 
     2881 GCTGTGTCAG CGGAGTCTGG TGGGCCTTCA CCTTGATCAT CATCTCCTCC TACACGGCCA 
     2941 ACCTGGCCGC CTTCCTCACC GTGCAGCGCA TGGAGGTGCC TGTGGAGTCG GCCGATGACC 
     3001 TGGCAGATCA GACCAACATC GAGTATGGCA CCATCCACGC CGGCTCCACC ATGACCTTCT 
     3061 TCCAGAATTC ACGGTACCAA ACGTACCAGC GCATGTGGAA CTACATGCAG TCGAAGCAGC 
     3121 CCAGCGTGTT CGTCAAGAGC ACAGAAGAGG GCATTGCCCG CGTCCTCAAC TCCCGCTACG 
     3181 CCTTCCTGCT CGAGTCCACC ATGAACGAAT ACCACCGGCG CCTCAACTGC AACCTCACCC 
     3241 AGATCGGGGG ACTCCTCGAC ACCAAGGGCT ACGGCATTGG CATGCCGCTG GGCTCCCCGT 
     3301 TCCGGGATGA GATCACACTG GCCATCCTGC AGCTTCAGGA GAACAACCGG CTGGAGATCC 
     3361 TGAAGCGCAA GTGGTGGGAG GGGGGCCGGT GCCCCAAGGA GGAGGACCAT CGAGCTAAAG 
     3421 GTTTGGGCAT GGAGAACATT GGTGGCATTT TTATCGTGCT CATCTGTGGC CTCATCATTG 
     3481 CTGTCTTCGT GGCGGTCATG GAATTCATAT GGTCCACACG GAGGTCAGCT GAGTCCGAGG 
     3541 AGGTGTCGGT GTGCCAGGAG ATGCTGCAGG AGCTGCGCCA CGCCGTTTCT TGCCGCAAGA 
     3601 CGTCGCGTTC CCGCCGGCGC CGACGCCCGG GCGGCCCGAG CCGGGCCCTG CTGTCACTGC 
     3661 GCGCGGTCCG CGAGATGCGC CTCAGCAACG GCAAGCTCTA CTCGGCCGGC GCGGGCGGGG 
     3721 ATGCGGGCAG CGCGCACGGG GGCCCGCAGC GCCTCCTGGA CGACCCGGGG CCCCCCAGCG 
     3781 GAGCCCGACC CGCCGCCCCC ACCCCCTGCA CCCACGTGCG CGTCTGCCAG GAGTGCCGGC 
     3841 GCATCCAGGC GCTGCGGGCC TCGGGGGCCG GCGCGCCTCC GCGTGGCCTG GGCGTCCCCG 
     3901 CCGAAGCCAC CAGCCCGCCC CGGCCGCGGC CTGGCCCCGC CGGCCCCCGG GAGCTGGCGG 
     3961 AGCACGAGAC GCGTACGCGG CCGCTCGAGA TGGAGAGCGA CGAGAGCGGC CTGCCCGCCA 
     4021 TGGAGATCGA GTGCCGCATC ACCGGCACCC TGAACGGCGT GGAGTTCGAG CTGGTGGGCG 
     4081 GCGGAGAGGG CACCCCCGAG CAGGGCCGCA TGACCAACAA GATGAAGAGC ACCAAAGGCG 
     4141 CCCTGACCTT CAGCCCCTAC CTGCTGAGCC ACGTGATGGG CTACGGCTTC TACCACTTCG 
     4201 GCACCTACCC CAGCGGCTAC GAGAACCCCT TCCTGCACGC CATCAACAAC GGCGGCTACA 
     4261 CCAACACCCG CATCGAGAAG TACGAGGACG GCGGCGTGCT GCACGTGAGC TTCAGCTACC 
     4321 GCTACGAGGC CGGCCGCGTG ATCGGCGACT TCAAGGTGAT GGGCACCGGC TTCCCCGAGG 
     4381 ACAGCGTGAT CTTCACCGAC AAGATCATCC GCAGCAACGC CACCGTGGAG CACCTGCACC 
     4441 CCATGGGCGA TAACGATCTG GATGGCAGCT TCACCCGCAC CTTCAGCCTG CGCGACGGCG 
     4501 GCTACTACAG CTCCGTGGTG GACAGCCACA TGCACTTCAA GAGCGCCATC CACCCCAGCA 
     4561 TCCTGCAGAA CGGGGGCCCC ATGTTCGCCT TCCGCCGCGT GGAGGAGGAT CACAGCAACA 
     4621 CCGAGCTGGG CATCGTGGAG TACCAGCACG CCTTCAAGAC CCCGGATGCA GATGCCGGTG 
     4681 AAGAAAGAGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4741 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4801 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4861 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4921 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4981 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     5041 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5101 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5161 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5221 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5281 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5341 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCTTGGCTGT GGAATGTGTG TCAGTTAGGG 
     5401 TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG 
     5461 TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 
     5521 CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT 
     5581 CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG 
     5641 GCCGAGGCCG CCTCGGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC 
     5701 CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAAGAG 
     5761 ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC 
     5821 GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT 
     5881 GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG 
     5941 TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG 
     6001 GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA 
     6061 TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA 
     6121 TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC 
     6181 GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC 
     6241 GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG 
     6301 CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG 
     6361 CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT 
     6421 GTGGCCGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC 
     6481 GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC 
     6541 ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG GACTCTGGGG TTCGAAATGA 
     6601 CCGACCAAGC GACGCCCAAC CTGCCATCAC GAGATTTCGA TTCCACCGCC GCCTTCTATG 
     6661 AAAGGTTGGG CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG 
     6721 ATCTCATGCT GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA 
     6781 AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT 
     6841 GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG ACCTCTAGCT 
     6901 AGAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA 
     6961 TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA 
     7021 GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT 
     7081 GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT 
     7141 CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT 
     7201 CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA 
     7261 ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT 
     7321 TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT 
     7381 GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC 
     7441 GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA 
     7501 GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT 
     7561 CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA 
     7621 ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG 
     7681 GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC 
     7741 CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA 
     7801 CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTT 
     7861 TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA 
     7921 TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA 
     7981 TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT 
     8041 CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG 
     8101 CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT 
     8161 AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG 
     8221 ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC 
     8281 GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG 
     8341 CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA 
     8401 TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA 
     8461 GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA 
     8521 TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA 
     8581 ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA 
     8641 AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG 
     8701 ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG 
     8761 GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG 
     8821 CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG 
     8881 GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC 
     8941 TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA 
     9001 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG 
     9061 TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA 
     9121 GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT 
     9181 TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT 
     9241 TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC 
     9301 GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT 
     9361 TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT 
     9421 TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC 
     9481 AAAAATTTAA CGCGAATTTT 
//