LOCUS       RG224605                8996 bp    DNA     circular  SYN 
DEFINITION  SUN1 (tGFP-tagged) - Human Sad1 and UNC84 domain containing 1 (SUN1), transcript variant 2
ACCESSION   NM_025154
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3477..4181 
                     /label="GFP"  
     misc_feature    4228..4813 
                     /label="PolyA signal"  
     misc_feature    6905..7550 
                     /label="ColE1"  
     misc_feature    5267..6061 
                     /label="Neo"  
     misc_feature    4898..5200 
                     /label="SV40 ori"  
     misc_feature    7565..8425 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3456..3462 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3456 
                     /label="SUN1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATTTTTCT CGGCTTCACA TGTACAGTCC TCCCCAGTGT GTGCCGGAGA 
     1081 ACACGGGCTA CACGTATGCG CTCAGTTCCA GCTATTCTTC AGATGCTCTG GATTTTGAGA 
     1141 CGGAGCACAA ATTGGACCCT GTATTTGATT CTCCACGGAT GTCCCGCCGT AGTTTGCGCC 
     1201 TGGCCACGAC AGCATGCACC CTGGGGGATG GTGAGGCTGT GGGTGCCGAC AGCGGCACCA 
     1261 GCAGCGCTGT CTCCCTGAAG AACCGAGCGG CCAGAACAAC AAAACAGCGC AGAAGCACAA 
     1321 ACAAATCAGC TTTTAGTATC AACCACGTGT CAAGGCAGGT CACGTCCTCT GGCGTCAGCT 
     1381 ACGGCGGCAC TGTCAGCCTG CAGGATGCTG TGACTCGACG GCCTCCTGTA TTGGACGAGT 
     1441 CTTGGATTCG TGAACAGACC ACAGTGGACC ACTTCTGGGG TCTTGATGAT GATGGTGATC 
     1501 TTAAAGGTGG AAATAAAGCT GCCATTCAGG GAAACGGGGA TGTGGGAGTC GCCGCCGCCA 
     1561 CCGCGCACAA CGGCTTCTCC TGCAGCAACT GCAGCATGCT GTCCGAGCGC AAGGACGTGC 
     1621 TCACGGCGCA CCCCGCGGCC CCCGGGCCCG TGTCGAGAGT TTATTCTAGG GACAGGAATC 
     1681 AAAAATGCGA CGACTGTAAG GGCAAGAGGC ACCTCGACGC GCACCCCGGT CGGGCAGGGA 
     1741 CCCTCTGGCA CATCTGGGCA TGTGCAGGTT ACTTCTTGCT GCAGATTCTG CGCAGGATCG 
     1801 GAGCTGTGGG CCAGGCTGTG TCCAGGACGG CGTGGTCGGC CCTTTGGCTG GCCGTGGTTG 
     1861 CTCCAGGGAA GGCAGCCTCT GGAGTGTTCT GGTGGCTGGG GATTGGATGG TACCAGTTTG 
     1921 TTACTTTGAT TTCTTGGCTG AATGTGTTTC TTCTTACCAG GTGCCTTCGA AACATCTGCA 
     1981 AGTTTTTAGT CTTGCTCATC CCACTCTTCC TTTTACTAGC AGGTCTCTCC TTACGGGGCC 
     2041 AGGGCAATTT CTTTTCGTTC TTGCCCGTGT TGAACTGGGC AAGCATGCAT AGAACACAGC 
     2101 GGGTGGATGA CCCCCAGGAC GTGTTTAAAC CCACGACTTC TCGCCTGAAG CAGCCTCTGC 
     2161 AGGGTGACAG TGAGGCTTTT CCGTGGCATT GGATGAGTGG CGTGGAGCAG CAGGTGGCCT 
     2221 CTCTGTCTGG ACAGTGCCAC CACCATGGTG AGAATCTCCG AGAGCTGACC ACTTTGCTAC 
     2281 AGAAGCTGCA GGCTCGGGTG GACCAGATGG AAGGCGGCGC TGCCGGGCCG TCAGCTTCGG 
     2341 TCAGAGACGC TGTGGGACAG CCCCCGAGGG AGACTGACTT TATGGCCTTT CACCAAGAAC 
     2401 ATGAAGTGCG TATGTCACAC TTGGAAGATA TTCTGGGAAA ACTGAGAGAA AAATCTGAGG 
     2461 CCATCCAGAA GGAACTAGAA CAGACCAAGC AAAAAACAAT CAGTGCGGTT GGTGAGCAGC 
     2521 TCCTGCCCAC AGTCGAGCAC CTCCAGCTGG AGCTGGATCA GCTAAAGTCA GAGCTGTCCA 
     2581 GCTGGCGACA CGTGAAGACC GGCTGTGAGA CAGTGGATGC CGTACAAGAA AGAGTGGACG 
     2641 TGCAAGTCAG AGAAATGGTG AAACTCCTGT TTTCCGAAGA TCAGCAAGGC GGTTCTCTGG 
     2701 AACAGCTGCT GCAGAGGTTC TCATCACAGT TTGTGAGCAA AGGCGACTTG CAGACGATGC 
     2761 TGCGAGACCT GCAGCTGCAG ATCCTGCGGA ACGTCACCCA CCACGTTTCC GTGACCAAGC 
     2821 AGCTCCCAAC CTCAGAAGCC GTGGTGTCTG CTGTGAGCGA GGCGGGGGCG TCTGGAATAA 
     2881 CAGAGGCGCA AGCACGTGCC ATCGTGAACA GCGCCTTGAA GCTGTATTCC CAAGATAAGA 
     2941 CCGGGATGGT GGACTTTGCT CTGGAATCTG GTGGTGGCAG CATCTTGAGT ACTCGCTGTT 
     3001 CTGAAACTTA CGAAACCAAA ACGGCGCTGA TGAGTCTGTT TGGGATCCCG CTGTGGTACT 
     3061 TCTCGCAGTC CCCGCGCGTG GTCATCCAGC CTGACATTTA CCCCGGTAAC TGCTGGGCAT 
     3121 TTAAAGGCTC CCAGGGGTAC CTGGTGGTGA GGCTCTCCAT GATGATCCAC CCAGCCGCCT 
     3181 TCACTCTGGA GCACATCCCT AAGACGCTGT CGCCAACAGG CAACATCAGC AGCGCCCCCA 
     3241 AGGACTTCGC CGTCTATGGA TTAGAAAATG AGTATCAGGA AGAAGGGCAG CTTCTGGGAC 
     3301 AGTTCACGTA TGATCAGGAT GGGGAGTCGC TCCAGATGTT CCAGGCCCTG AAAAGACCCG 
     3361 ACGACACAGC TTTCCAAATA GTGGAACTTC GGATTTTTTC TAACTGGGGC CATCCTGAGT 
     3421 ATACCTGTCT GTATCGGTTC AGAGTTCATG GCGAACCTGT CAAGACGCGT ACGCGGCCGC 
     3481 TCGAGATGGA GAGCGACGAG AGCGGCCTGC CCGCCATGGA GATCGAGTGC CGCATCACCG 
     3541 GCACCCTGAA CGGCGTGGAG TTCGAGCTGG TGGGCGGCGG AGAGGGCACC CCCGAGCAGG 
     3601 GCCGCATGAC CAACAAGATG AAGAGCACCA AAGGCGCCCT GACCTTCAGC CCCTACCTGC 
     3661 TGAGCCACGT GATGGGCTAC GGCTTCTACC ACTTCGGCAC CTACCCCAGC GGCTACGAGA 
     3721 ACCCCTTCCT GCACGCCATC AACAACGGCG GCTACACCAA CACCCGCATC GAGAAGTACG 
     3781 AGGACGGCGG CGTGCTGCAC GTGAGCTTCA GCTACCGCTA CGAGGCCGGC CGCGTGATCG 
     3841 GCGACTTCAA GGTGATGGGC ACCGGCTTCC CCGAGGACAG CGTGATCTTC ACCGACAAGA 
     3901 TCATCCGCAG CAACGCCACC GTGGAGCACC TGCACCCCAT GGGCGATAAC GATCTGGATG 
     3961 GCAGCTTCAC CCGCACCTTC AGCCTGCGCG ACGGCGGCTA CTACAGCTCC GTGGTGGACA 
     4021 GCCACATGCA CTTCAAGAGC GCCATCCACC CCAGCATCCT GCAGAACGGG GGCCCCATGT 
     4081 TCGCCTTCCG CCGCGTGGAG GAGGATCACA GCAACACCGA GCTGGGCATC GTGGAGTACC 
     4141 AGCACGCCTT CAAGACCCCG GATGCAGATG CCGGTGAAGA AAGAGTTTAA ACGGCCGGCC 
     4201 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4261 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4321 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4381 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4441 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4501 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4561 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4621 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     4681 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     4741 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     4801 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     4861 CGGCCAGCTT GGCTGTGGAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA 
     4921 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCAGGTG TGGAAAGTCC 
     4981 CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCATA 
     5041 GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG 
     5101 CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC GGCCTCTGAG 
     5161 CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG 
     5221 GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG 
     5281 ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC 
     5341 TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG 
     5401 CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG 
     5461 GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC 
     5521 GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT 
     5581 CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG 
     5641 CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC 
     5701 GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG 
     5761 CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC 
     5821 GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC 
     5881 CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CCGACCGCTA TCAGGACATA 
     5941 GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC 
     6001 GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC 
     6061 GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC 
     6121 CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT 
     6181 TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC 
     6241 ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT 
     6301 TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG 
     6361 TATCTTATCA TGTCTGTATA CCGTCGACCT CTAGCTAGAG CTTGGCGTAA TCATGGTCAT 
     6421 AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA 
     6481 GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC 
     6541 GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC 
     6601 AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT 
     6661 CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC 
     6721 GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA 
     6781 AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG 
     6841 ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA 
     6901 GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC 
     6961 TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC 
     7021 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC 
     7081 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG 
     7141 TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT 
     7201 ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA 
     7261 CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT 
     7321 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTTTTTT TGTTTGCAAG CAGCAGATTA 
     7381 CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC 
     7441 AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA 
     7501 CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA 
     7561 CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT 
     7621 TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT 
     7681 TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT 
     7741 TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT 
     7801 CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA 
     7861 ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG 
     7921 GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT 
     7981 TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG 
     8041 CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG 
     8101 TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC 
     8161 GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA 
     8221 CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC 
     8281 CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT 
     8341 TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG 
     8401 GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA 
     8461 GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA 
     8521 AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG 
     8581 GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG 
     8641 CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC 
     8701 CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC 
     8761 TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA 
     8821 CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA 
     8881 CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA 
     8941 TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//