LOCUS       RG224278                9167 bp    DNA     circular  SYN 
DEFINITION  FGA (tGFP-tagged) - Human fibrinogen alpha chain (FGA), transcript variant alpha-E
ACCESSION   NM_000508
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3646..4350 
                     /label="GFP"  
     misc_feature    4397..4982 
                     /label="PolyA signal"  
     misc_feature    7074..7719 
                     /label="ColE1"  
     misc_feature    5436..6230 
                     /label="Neo"  
     misc_feature    5067..5369 
                     /label="SV40 ori"  
     misc_feature    7734..8594 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3618..3625 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3618 
                     /label="FGA"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTTTCCATG AGGATCGTCT GCCTGGTCCT AAGTGTGGTG GGCACAGCAT 
     1081 GGACTGCAGA TAGTGGTGAA GGTGACTTTC TAGCTGAAGG AGGAGGCGTG CGTGGCCCAA 
     1141 GGGTTGTGGA AAGACATCAA TCTGCCTGCA AAGATTCAGA CTGGCCCTTC TGCTCTGATG 
     1201 AAGACTGGAA CTACAAATGC CCTTCTGGCT GCAGGATGAA AGGGTTGATT GATGAAGTCA 
     1261 ATCAAGATTT TACAAACAGA ATAAATAAGC TCAAAAATTC ACTATTTGAA TATCAGAAGA 
     1321 ACAATAAGGA TTCTCATTCG TTGACCACTA ATATAATGGA AATTTTGAGA GGCGATTTTT 
     1381 CCTCAGCCAA TAACCGTGAT AATACCTACA ACCGAGTGTC AGAGGATCTG AGAAGCAGAA 
     1441 TTGAAGTCCT GAAGCGCAAA GTCATAGAAA AAGTACAGCA TATCCAGCTT CTGCAGAAAA 
     1501 ATGTTAGAGC TCAGTTGGTT GATATGAAAC GACTGGAGGT GGACATTGAT ATTAAGATCC 
     1561 GATCTTGTCG AGGGTCATGC AGTAGGGCTT TAGCTCGTGA AGTAGATCTG AAGGACTATG 
     1621 AAGATCAGCA GAAGCAACTT GAACAGGTCA TTGCCAAAGA CTTACTTCCC TCTAGAGATA 
     1681 GGCAACACTT ACCACTGATA AAAATGAAAC CAGTTCCAGA CTTGGTTCCC GGAAATTTTA 
     1741 AGAGCCAGCT TCAGAAGGTA CCCCCAGAGT GGAAGGCATT AACAGACATG CCGCAGATGA 
     1801 GAATGGAGTT AGAGAGACCT GGTGGAAATG AGATTACTCG AGGAGGCTCC ACCTCTTATG 
     1861 GAACCGGATC AGAGACGGAA AGCCCCAGGA ACCCTAGCAG TGCTGGAAGC TGGAACTCTG 
     1921 GGAGCTCTGG ACCTGGAAGT ACTGGAAACC GAAACCCTGG GAGCTCTGGG ACTGGAGGGA 
     1981 CTGCAACCTG GAAACCTGGG AGCTCTGGAC CTGGAAGTAC TGGAAGCTGG AACTCTGGGA 
     2041 GCTCTGGAAC TGGAAGTACT GGAAACCAAA ACCCTGGGAG CCCTAGACCT GGTAGTACCG 
     2101 GAACCTGGAA TCCTGGCAGC TCTGAACGCG GAAGTGCTGG GCACTGGACC TCTGAGAGCT 
     2161 CTGTATCTGG TAGTACTGGA CAATGGCACT CTGAATCTGG AAGTTTTAGG CCAGATAGCC 
     2221 CAGGCTCTGG GAACGCGAGG CCTAACAACC CAGACTGGGG CACATTTGAA GAGGTGTCAG 
     2281 GAAATGTAAG TCCAGGGACA AGGAGAGAGT ACCACACAGA AAAACTGGTC ACTTCTAAAG 
     2341 GAGATAAAGA GCTCAGGACT GGTAAAGAGA AGGTCACCTC TGGTAGCACA ACCACCACGC 
     2401 GTCGTTCATG CTCTAAAACC GTTACTAAGA CTGTTATTGG TCCTGATGGT CACAAAGAAG 
     2461 TTACCAAAGA AGTGGTGACC TCCGAAGATG GTTCTGACTG TCCCGAGGCA ATGGATTTAG 
     2521 GCACATTGTC TGGCATAGGT ACTCTGGATG GGTTCCGCCA TAGGCACCCT GATGAAGCTG 
     2581 CCTTCTTCGA CACTGCCTCA ACTGGAAAAA CATTCCCAGG TTTCTTCTCA CCTATGTTAG 
     2641 GAGAGTTTGT CAGTGAGACT GAGTCTAGGG GCTCAGAATC TGGCATCTTC ACAAATACAA 
     2701 AGGAATCCAG TTCTCATCAC CCTGGGATAG CTGAATTCCC TTCCCGTGGT AAATCTTCAA 
     2761 GTTACAGCAA ACAATTTACT AGTAGCACGA GTTACAACAG AGGAGACTCC ACATTTGAAA 
     2821 GCAAGAGCTA TAAAATGGCA GATGAGGCCG GAAGTGAAGC CGATCATGAA GGAACACATA 
     2881 GCACCAAGAG AGGCCATGCT AAATCTCGCC CTGTCAGAGA CTGTGATGAT GTCCTCCAAA 
     2941 CACATCCTTC AGGTACCCAA AGTGGCATTT TCAATATCAA GCTACCGGGA TCCAGTAAGA 
     3001 TTTTTTCTGT TTATTGCGAT CAAGAGACCA GTTTGGGAGG ATGGCTTTTG ATCCAGCAAA 
     3061 GAATGGATGG ATCACTGAAT TTTAACCGGA CCTGGCAAGA CTACAAGAGA GGTTTCGGCA 
     3121 GCCTGAATGA CGAGGGGGAA GGAGAATTCT GGCTAGGCAA TGACTACCTC CACTTACTAA 
     3181 CCCAAAGGGG CTCTGTTCTT AGGGTTGAAT TAGAGGACTG GGCTGGGAAT GAAGCTTATG 
     3241 CAGAATATCA CTTCCGGGTA GGCTCTGAGG CTGAAGGCTA TGCCCTCCAA GTCTCCTCCT 
     3301 ATGAAGGCAC TGCGGGTGAT GCTCTGATTG AGGGTTCCGT AGAGGAAGGG GCAGAGTACA 
     3361 CCTCTCACAA CAACATGCAG TTCAGCACCT TTGACAGGGA TGCAGACCAG TGGGAAGAGA 
     3421 ACTGTGCAGA AGTCTATGGG GGAGGCTGGT GGTATAATAA CTGCCAAGCA GCCAATCTCA 
     3481 ATGGAATCTA CTACCCTGGG GGCTCCTATG ACCCAAGGAA TAACAGTCCT TATGAGATTG 
     3541 AGAATGGAGT GGTCTGGGTT TCCTTTAGAG GGGCAGATTA TTCCCTCAGG GCTGTTCGCA 
     3601 TGAAAATTAG GCCCCTTGTG ACCCAAAGCG GACCGACGCG TACGCGGCCG CTCGAGATGG 
     3661 AGAGCGACGA GAGCGGCCTG CCCGCCATGG AGATCGAGTG CCGCATCACC GGCACCCTGA 
     3721 ACGGCGTGGA GTTCGAGCTG GTGGGCGGCG GAGAGGGCAC CCCCGAGCAG GGCCGCATGA 
     3781 CCAACAAGAT GAAGAGCACC AAAGGCGCCC TGACCTTCAG CCCCTACCTG CTGAGCCACG 
     3841 TGATGGGCTA CGGCTTCTAC CACTTCGGCA CCTACCCCAG CGGCTACGAG AACCCCTTCC 
     3901 TGCACGCCAT CAACAACGGC GGCTACACCA ACACCCGCAT CGAGAAGTAC GAGGACGGCG 
     3961 GCGTGCTGCA CGTGAGCTTC AGCTACCGCT ACGAGGCCGG CCGCGTGATC GGCGACTTCA 
     4021 AGGTGATGGG CACCGGCTTC CCCGAGGACA GCGTGATCTT CACCGACAAG ATCATCCGCA 
     4081 GCAACGCCAC CGTGGAGCAC CTGCACCCCA TGGGCGATAA CGATCTGGAT GGCAGCTTCA 
     4141 CCCGCACCTT CAGCCTGCGC GACGGCGGCT ACTACAGCTC CGTGGTGGAC AGCCACATGC 
     4201 ACTTCAAGAG CGCCATCCAC CCCAGCATCC TGCAGAACGG GGGCCCCATG TTCGCCTTCC 
     4261 GCCGCGTGGA GGAGGATCAC AGCAACACCG AGCTGGGCAT CGTGGAGTAC CAGCACGCCT 
     4321 TCAAGACCCC GGATGCAGAT GCCGGTGAAG AAAGAGTTTA AACGGCCGGC CGCGGTCATA 
     4381 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4441 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4501 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4561 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4621 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4681 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     4741 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     4801 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     4861 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     4921 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     4981 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCT 
     5041 TGGCTGTGGA ATGTGTGTCA GTTAGGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA 
     5101 AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCAGGT GTGGAAAGTC CCCAGGCTCC 
     5161 CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAT AGTCCCGCCC 
     5221 CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC 
     5281 TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CGGCCTCTGA GCTATTCCAG 
     5341 AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT 
     5401 ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA 
     5461 GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG 
     5521 GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC 
     5581 CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA 
     5641 GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC 
     5701 ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA 
     5761 TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT 
     5821 ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA 
     5881 CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG 
     5941 CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGAGGATCTC 
     6001 GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT 
     6061 GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCCGACCGCT ATCAGGACAT AGCGTTGGCT 
     6121 ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC 
     6181 GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC 
     6241 TGAGCGGGAC TCTGGGGTTC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG 
     6301 ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG 
     6361 CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCCAACT 
     6421 TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA 
     6481 AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC 
     6541 ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC 
     6601 CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT 
     6661 GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC 
     6721 CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG 
     6781 GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT 
     6841 CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA 
     6901 CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA 
     6961 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC 
     7021 ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG 
     7081 CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT 
     7141 ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT 
     7201 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC 
     7261 AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG 
     7321 ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG 
     7381 GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG 
     7441 GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG 
     7501 GCAAACAAAC CACCGCTGGT AGCGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA 
     7561 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG 
     7621 AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC 
     7681 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG 
     7741 ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT 
     7801 CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG 
     7861 GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA 
     7921 TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA 
     7981 TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC 
     8041 GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT 
     8101 CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA 
     8161 AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT 
     8221 CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT 
     8281 TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA 
     8341 GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG 
     8401 TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA 
     8461 GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA 
     8521 CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG 
     8581 CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC 
     8641 AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG 
     8701 GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA 
     8761 GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC 
     8821 CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG 
     8881 CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA 
     8941 AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC 
     9001 GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA 
     9061 CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT 
     9121 ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//