LOCUS       RG223941                9212 bp    DNA     circular  SYN 
DEFINITION  GRIA4 (tGFP-tagged) - Human glutamate receptor, ionotrophic, AMPA 4 (GRIA4), transcript variant 2
ACCESSION   NM_001077243
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3693..4397 
                     /label="GFP"  
     misc_feature    4444..5029 
                     /label="PolyA signal"  
     misc_feature    7121..7766 
                     /label="ColE1"  
     misc_feature    5483..6277 
                     /label="Neo"  
     misc_feature    5114..5416 
                     /label="SV40 ori"  
     misc_feature    7781..8641 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3672..3678 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3672 
                     /label="GRIA4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGGATTATT TCCAGACAGA TTGTCTTGTT ATTTTCTGGA TTTTGGGGAC 
     1081 TCGCCATGGG AGCCTTTCCG AGCAGCGTGC AAATAGGTGG TCTCTTCATC CGAAACACAG 
     1141 ATCAGGAATA CACTGCTTTT CGATTAGCAA TTTTTCTTCA TAACACCAGC CCCAATGCGT 
     1201 CGGAAGCTCC TTTTAATTTG GTACCTCATG TGGACAACAT TGAGACAGCC AACAGTTTTG 
     1261 CTGTAACAAA CGCCTTCTGT TCCCAGTATT CTAGAGGAGT ATTTGCCATT TTTGGACTCT 
     1321 ATGATAAGAG GTCGGTACAT ACCTTGACCT CATTCTGCAG CGCCTTACAT ATCTCCCTCA 
     1381 TCACACCAAG TTTCCCTACT GAGGGGGAGA GCCAGTTTGT GCTGCAACTA AGACCTTCGT 
     1441 TACGAGGAGC ACTCTTGAGT TTGCTGGATC ACTACGAATG GAACTGTTTT GTCTTCCTGT 
     1501 ATGACACAGA CAGGGGATAC TCGATACTCC AAGCTATTAT GGAAAAAGCA GGACAAAATG 
     1561 GTTGGCATGT CAGCGCTATA TGTGTGGAAA ATTTTAATGA TGTCAGCTAT AGGCAACTTC 
     1621 TAGAAGAACT TGACAGAAGA CAAGAGAAGA AGTTTGTAAT AGACTGTGAG ATAGAGAGAC 
     1681 TTCAAAACAT ATTAGAACAG ATTGTAAGTG TTGGAAAGCA TGTTAAAGGC TACCATTATA 
     1741 TCATTGCAAA CTTGGGATTC AAGGATATTT CTCTTGAGAG GTTTATACAT GGTGGAGCCA 
     1801 ATGTTACTGG ATTCCAGTTG GTGGATTTTA ATACACCTAT GGTAATCAAA CTAATGGATC 
     1861 GCTGGAAGAA ACTAGATCAG AGAGAGTATC CAGGATCTGA GACTCCTCCA AAGTACACCT 
     1921 CTGCTCTGAC TTATGATGGA GTCCTTGTGA TGGCTGAAAC TTTCCGAAGT CTTAGGAGGC 
     1981 AGAAAATTGA TATCTCAAGG AGAGGAAATG CTGGGGATTG TCTGGCAAAT CCTGCTGCTC 
     2041 CATGGGGCCA GGGAATTGAC ATGGAGAGGA CACTCAAACA GGTTCGAATT CAAGGGCTGA 
     2101 CAGGGAATGT TCAGTTTGAC CACTATGGAC GTAGAGTCAA TTACACAATG GATGTGTTTG 
     2161 AGCTGAAAAG CACAGGACCT AGAAAGGTTG GTTACTGGAA TGATATGGAT AAGTTAGTCT 
     2221 TGATTCAAGA TGTACCAACT CTTGGCAATG ACACAGCTGC TATTGAGAAC AGAACAGTGG 
     2281 TTGTAACCAC AATTATGGAA TCCCCATATG TTATGTACAA GAAAAATCAT GAAATGTTTG 
     2341 AAGGAAATGA CAAGTATGAA GGATACTGTG TAGATTTGGC ATCTGAAATT GCAAAACATA 
     2401 TTGGTATCAA GTATAAAATT GCCATTGTCC CTGATGGAAA ATATGGAGCA AGGGATGCAG 
     2461 ACACAAAAAT CTGGAATGGG ATGGTAGGAG AACTTGTTTA TGGGAAAGCA GAGATTGCTA 
     2521 TTGCCCCTCT GACAATCACT TTGGTACGAG AGGAGGTCAT TGACTTTTCT AAGCCCTTCA 
     2581 TGAGTTTGGG CATATCTATC ATGATCAAAA AGCCTCAGAA ATCCAAACCA GGAGTGTTTT 
     2641 CCTTCTTGGA TCCTCTGGCC TATGAGATTT GGATGTGCAT AGTCTTTGCC TACATTGGTG 
     2701 TCAGCGTGGT CTTATTCCTA GTTAGTAGAT TTAGTCCATA TGAGTGGCAC ACAGAAGAGC 
     2761 CAGAGGACGG AAAGGAAGGA CCCAGCGACC AGCCTCCCAA TGAGTTTGGC ATCTTTAACA 
     2821 GCCTCTGGTT TTCCCTGGGT GCTTTTATGC AGCAAGGATG TGACATTTCA CCCAGATCCC 
     2881 TCTCAGGTCG AATTGTTGGA GGTGTTTGGT GGTTCTTTAC ACTCATCATT ATATCATCTT 
     2941 ATACTGCTAA CCTCGCTGCT TTCCTGACGG TTGAGCGAAT GGTCTCTCCC ATAGAAAGTG 
     3001 CAGAAGACCT GGCCAAACAA ACAGAAATTG CCTATGGAAC ACTGGATTCA GGATCAACAA 
     3061 AAGAATTCTT CAGAAGATCA AAAATAGCAG TGTATGAAAA GATGTGGACC TACATGCGAT 
     3121 CAGCAGAGCC ATCAGTATTC ACTAGGACTA CAGCTGAGGG AGTAGCTCGT GTCCGCAAAT 
     3181 CCAAGGGCAA ATTTGCCTTT CTCCTGGAGT CCACTATGAA TGAATACATT GAGCAGCGAA 
     3241 AGCCATGTGA CACGATGAAA GTGGGAGGAA ATCTGGATTC CAAAGGCTAT GGAGTAGCAA 
     3301 CGCCCAAGGG TTCCTCATTA GGAAATGCTG TTAACCTCGC AGTTTTAAAA CTGAATGAAC 
     3361 AAGGCCTCTT GGACAAATTG AAAAACAAAT GGTGGTACGA CAAAGGAGAA TGTGGCAGCG 
     3421 GGGGAGGTGA CTCCAAGGAC AAGACGAGTG CCTTGAGCCT GAGCAATGTA GCAGGCGTCT 
     3481 TCTACATTCT GGTTGGCGGC TTGGGCTTGG CAATGCTGGT GGCTTTGATA GAGTTCTGTT 
     3541 ACAAGTCCAG GGCAGAAGCG AAGAGAATGA AGGTGGCAAA GAGTGCACAG ACTTTTAACC 
     3601 CAACTTCCTC GCAGAATACC CAGAATTTAG CAACCTATAG AGAAGGTTAC AACGTATATG 
     3661 GAACCGAAAG TATTAAAATT ACGCGTACGC GGCCGCTCGA GATGGAGAGC GACGAGAGCG 
     3721 GCCTGCCCGC CATGGAGATC GAGTGCCGCA TCACCGGCAC CCTGAACGGC GTGGAGTTCG 
     3781 AGCTGGTGGG CGGCGGAGAG GGCACCCCCG AGCAGGGCCG CATGACCAAC AAGATGAAGA 
     3841 GCACCAAAGG CGCCCTGACC TTCAGCCCCT ACCTGCTGAG CCACGTGATG GGCTACGGCT 
     3901 TCTACCACTT CGGCACCTAC CCCAGCGGCT ACGAGAACCC CTTCCTGCAC GCCATCAACA 
     3961 ACGGCGGCTA CACCAACACC CGCATCGAGA AGTACGAGGA CGGCGGCGTG CTGCACGTGA 
     4021 GCTTCAGCTA CCGCTACGAG GCCGGCCGCG TGATCGGCGA CTTCAAGGTG ATGGGCACCG 
     4081 GCTTCCCCGA GGACAGCGTG ATCTTCACCG ACAAGATCAT CCGCAGCAAC GCCACCGTGG 
     4141 AGCACCTGCA CCCCATGGGC GATAACGATC TGGATGGCAG CTTCACCCGC ACCTTCAGCC 
     4201 TGCGCGACGG CGGCTACTAC AGCTCCGTGG TGGACAGCCA CATGCACTTC AAGAGCGCCA 
     4261 TCCACCCCAG CATCCTGCAG AACGGGGGCC CCATGTTCGC CTTCCGCCGC GTGGAGGAGG 
     4321 ATCACAGCAA CACCGAGCTG GGCATCGTGG AGTACCAGCA CGCCTTCAAG ACCCCGGATG 
     4381 CAGATGCCGG TGAAGAAAGA GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4441 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4501 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4561 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4621 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4681 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4741 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4801 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4861 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4921 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     4981 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5041 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCTTGGCT GTGGAATGTG 
     5101 TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 
     5161 CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 
     5221 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC 
     5281 CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT 
     5341 ATTTATGCAG AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC 
     5401 TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA 
     5461 TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA 
     5521 GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC 
     5581 GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC 
     5641 AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG 
     5701 CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG 
     5761 GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT 
     5821 GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT 
     5881 ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA 
     5941 GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA 
     6001 CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC 
     6061 GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT 
     6121 GGCCGGCTGG GTGTGGCCGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT 
     6181 GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC 
     6241 GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG 
     6301 GGTTCGAAAT GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG 
     6361 CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC 
     6421 TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT 
     6481 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC 
     6541 TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT 
     6601 CGACCTCTAG CTAGAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT 
     6661 ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG 
     6721 CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG 
     6781 GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC 
     6841 GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC 
     6901 GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA 
     6961 ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG 
     7021 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT 
     7081 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA 
     7141 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC 
     7201 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT 
     7261 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG 
     7321 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG 
     7381 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT 
     7441 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC 
     7501 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG 
     7561 CTGGTAGCGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG 
     7621 AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG 
     7681 GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT 
     7741 GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT 
     7801 TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC 
     7861 TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA 
     7921 TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG 
     7981 GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT 
     8041 GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA 
     8101 TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT 
     8161 CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT 
     8221 TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG 
     8281 CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG 
     8341 AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG 
     8401 CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA 
     8461 AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT 
     8521 AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT 
     8581 GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT 
     8641 GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA 
     8701 TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT 
     8761 TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG 
     8821 TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT 
     8881 TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC 
     8941 TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG 
     9001 GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG 
     9061 AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT 
     9121 CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG 
     9181 AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//