LOCUS       RG223861                9062 bp    DNA     circular  SYN 
DEFINITION  PRICKLE1 (tGFP-tagged) - Human prickle homolog 1 (Drosophila) (PRICKLE1), transcript variant 1
ACCESSION   NM_153026
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3541..4245 
                     /label="GFP"  
     misc_feature    4292..4877 
                     /label="PolyA signal"  
     misc_feature    6969..7614 
                     /label="ColE1"  
     misc_feature    5331..6125 
                     /label="Neo"  
     misc_feature    4962..5264 
                     /label="SV40 ori"  
     misc_feature    7629..8489 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3513..3520 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3513 
                     /label="PRICKLE1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTTTGGAG ATGGAGCCCA AGATGAGCAA ACTGGCCTTT GGCTGTCAGA 
     1081 GAAGTTCCAC ATCAGATGAT GACTCTGGCT GTGCATTGGA GGAGTACGCC TGGGTCCCCC 
     1141 CGGGCCTGAG ACCAGAGCAG ATCCAGCTCT ATTTTGCTTG CTTACCAGAG GAAAAAGTTC 
     1201 CTTACGTTAA CAGCCCCGGA GAGAAGCATC GGATTAAACA GCTTTTGTAC CAGTTACCAC 
     1261 CACATGATAA TGAGGTACGG TATTGCCAGT CTTTGAGTGA AGAGGAGAAA AAAGAGTTGC 
     1321 AGGTGTTCAG TGCTCAGCGG AAGAAAGAAG CACTGGGAAG AGGAACAATT AAGCTTCTGT 
     1381 CCAGAGCAGT CATGCATGCT GTGTGTGAGC AGTGTGGTTT GAAGATAAAT GGAGGTGAAG 
     1441 TTGCAGTGTT CGCCTCCCGT GCGGGCCCTG GTGTGTGCTG GCACCCATCC TGTTTTGTCT 
     1501 GTTTCACGTG TAATGAGCTG CTGGTCGACC TCATCTATTT TTATCAGGAT GGAAAAATTC 
     1561 ACTGTGGCAG GCACCATGCA GAACTGCTCA AACCACGGTG CTCAGCATGT GACGAGATAA 
     1621 TTTTTGCTGA TGAATGCACA GAAGCTGAGG GTCGCCATTG GCACATGAAA CACTTCTGCT 
     1681 GCCTTGAGTG TGAAACGGTC CTGGGAGGAC AGAGGTATAT CATGAAGGAC GGCCGCCCCT 
     1741 TCTGCTGTGG CTGTTTTGAG TCTCTCTATG CGGAGTACTG TGAAACCTGT GGGGAACATA 
     1801 TTGGTGTGGA CCATGCACAG ATGACCTATG ACGGGCAGCA CTGGCACGCC ACGGAAGCCT 
     1861 GCTTTTCTTG TGCCCAGTGT AAAGCCTCTT TGTTGGGATG TCCCTTCCTT CCCAAACAGG 
     1921 GTCAGATTTA CTGCTCAAAA ACGTGCAGTC TTGGTGAAGA CGTCCATGCC TCTGATTCTT 
     1981 CCGACTCTGC ATTTCAGTCA GCTCGATCAA GAGACTCCCG AAGAAGTGTC CGAATGGGCA 
     2041 AGAGCAGCCG GTCAGCAGAT CAGTGTAGAC AGTCTCTCCT CTTATCGCCT GCTCTGAACT 
     2101 ACAAGTTTCC TGGCCTCTCA GGCAATGCTG ATGACACCCT TTCTCGAAAA TTGGATGATC 
     2161 TGAGTCTCTC CAGACAAGGA ACAAGTTTTG CCAGTGAAGA ATTTTGGAAA GGCAGAGTAG 
     2221 AGCAGGAAAC TCCAGAAGAC CCTGAAGAAT GGGCTGATCA TGAAGATTAT ATGACGCAGC 
     2281 TCCTCCTCAA GTTTGGTGAT AAAAGCCTCT TTCAGCCACA GCCCAATGAG ATGGATATTC 
     2341 GAGCCAGTGA GCACTGGATA TCTGATAACA TGGTTAAAAG TAAGACCGAG TTAAAGCAAA 
     2401 ATAACCAGAG CCTTGCAAGT AAAAAATACC AGTCTGATAT GTACTGGGCA CAGTCACAAG 
     2461 ATGGACTGGG CGATTCTGCT TATGGCAGCC ACCCAGGCCC TGCAAGCAGT AGAAGGCTTC 
     2521 AGGAATTGGA ACTGGACCAT GGGGCTTCAG GGTATAATCA TGATGAAACA CAGTGGTATG 
     2581 AAGATTCCCT GGAGTGTCTG TCAGACCTGA AACCAGAGCA AAGTGTTCGG GATTCGATGG 
     2641 ATTCTTTGGC ATTGTCCAAT ATCACAGGGG CTTCGGTGGA TGGAGAAAAC AAGCCAAGGC 
     2701 CATCATTGTA TTCTCTGCAA AATTTTGAGG AGATGGAAAC AGAAGATTGT GAGAAGATGA 
     2761 GCAATATGGG AACTTTGAAC TCTTCCATGC TGCACAGGAG TGCAGAGTCC TTAAAGAGTC 
     2821 TAAGTTCAGA GTTGTGTCCA GAGAAAATCC TGCCTGAAGA GAAGCCAGTA CATCTGCCAG 
     2881 TGCTCAGAAG GTCCAAGTCT CAATCCAGAC CCCAGCAGGT CAAGTTCTCC GATGATGTCA 
     2941 TTGACAATGG GAACTATGAC ATTGAAATCC GGCAGCCTCC GATGAGTGAA AGGACTCGGA 
     3001 GACGCGTCTA CAATTTTGAA GAGAGGGGAT CCAGGTCTCA TCACCACCGC CGCCGGAGAA 
     3061 GTAGAAAGTC CCGCTCCGAC AATGCCCTGA ATCTTGTTAC AGAAAGAAAA TACTCTCCCA 
     3121 AGGACAGACT GCGGCTGTAC ACCCCCGATA ACTATGAGAA ATTTATACAG AATAAAAGTG 
     3181 CCCGGGAGAT CCAAGCATAC ATCCAGAATG CTGATCTCTA CGGACAGTAC GCCCATGCCA 
     3241 CTCCCGATTA TGGCCTGCAG AACCCAGGAA TGAATCGGTT TCTGGGACTC TACGGCGAGG 
     3301 ATGATGATTC CTGGTGTTCT TCCTCCTCCT CCTCTTCCGA CTCGGAAGAA GAAGGATATT 
     3361 TTCTTGGACA ACCAATCCCT CAACCCCGGC CACAGAGATT TGCCTACTAT ACAGATGACC 
     3421 TTTCTAGTCC ACCATCTGCA CTTCCCACCC CTCAGTTTGG TCAGAGGACA ACAAAATCCA 
     3481 AGAAGAAAAA GGGACACAAG GGCAAAAATT GTATTATTTC TAGCGGACCG ACGCGTACGC 
     3541 GGCCGCTCGA GATGGAGAGC GACGAGAGCG GCCTGCCCGC CATGGAGATC GAGTGCCGCA 
     3601 TCACCGGCAC CCTGAACGGC GTGGAGTTCG AGCTGGTGGG CGGCGGAGAG GGCACCCCCG 
     3661 AGCAGGGCCG CATGACCAAC AAGATGAAGA GCACCAAAGG CGCCCTGACC TTCAGCCCCT 
     3721 ACCTGCTGAG CCACGTGATG GGCTACGGCT TCTACCACTT CGGCACCTAC CCCAGCGGCT 
     3781 ACGAGAACCC CTTCCTGCAC GCCATCAACA ACGGCGGCTA CACCAACACC CGCATCGAGA 
     3841 AGTACGAGGA CGGCGGCGTG CTGCACGTGA GCTTCAGCTA CCGCTACGAG GCCGGCCGCG 
     3901 TGATCGGCGA CTTCAAGGTG ATGGGCACCG GCTTCCCCGA GGACAGCGTG ATCTTCACCG 
     3961 ACAAGATCAT CCGCAGCAAC GCCACCGTGG AGCACCTGCA CCCCATGGGC GATAACGATC 
     4021 TGGATGGCAG CTTCACCCGC ACCTTCAGCC TGCGCGACGG CGGCTACTAC AGCTCCGTGG 
     4081 TGGACAGCCA CATGCACTTC AAGAGCGCCA TCCACCCCAG CATCCTGCAG AACGGGGGCC 
     4141 CCATGTTCGC CTTCCGCCGC GTGGAGGAGG ATCACAGCAA CACCGAGCTG GGCATCGTGG 
     4201 AGTACCAGCA CGCCTTCAAG ACCCCGGATG CAGATGCCGG TGAAGAAAGA GTTTAAACGG 
     4261 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4321 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4381 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4441 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4501 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4561 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4621 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4681 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4741 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4801 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4861 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4921 GGTACGCGGC CAGCTTGGCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC 
     4981 TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA 
     5041 AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA 
     5101 ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT 
     5161 TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCGGCC 
     5221 TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG 
     5281 CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT 
     5341 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA 
     5401 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG 
     5461 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA 
     5521 CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT 
     5581 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG 
     5641 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA 
     5701 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT 
     5761 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC 
     5821 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC 
     5881 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA 
     5941 AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCCGA CCGCTATCAG 
     6001 GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC 
     6061 TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT 
     6121 CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGAAAT GACCGACCAA GCGACGCCCA 
     6181 ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA 
     6241 TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG CTGGAGTTCT 
     6301 TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA 
     6361 CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA 
     6421 TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG GCGTAATCAT 
     6481 GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG 
     6541 CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG 
     6601 CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA 
     6661 TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA 
     6721 CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG 
     6781 TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC 
     6841 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC 
     6901 CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC 
     6961 TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC 
     7021 TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA 
     7081 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC 
     7141 ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA 
     7201 ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG 
     7261 CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA 
     7321 GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG 
     7381 GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TTTTTTTGTT TGCAAGCAGC 
     7441 AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG 
     7501 ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA 
     7561 TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG 
     7621 AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT 
     7681 GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG 
     7741 AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC 
     7801 CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA 
     7861 CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC 
     7921 CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT 
     7981 CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC 
     8041 CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT 
     8101 TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC 
     8161 CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT 
     8221 GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA 
     8281 GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA 
     8341 TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG 
     8401 CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA 
     8461 AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT 
     8521 ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA 
     8581 AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT 
     8641 GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG 
     8701 CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG 
     8761 GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC 
     8821 GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT 
     8881 GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT 
     8941 TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT 
     9001 TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT 
     9061 TT
//