LOCUS       RG223738                9305 bp    DNA     circular  SYN 
DEFINITION  OSBP2 (tGFP-tagged) - Human oxysterol binding protein 2 (OSBP2), transcript variant 1
ACCESSION   NM_030758
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3786..4490 
                     /label="GFP"  
     misc_feature    4537..5122 
                     /label="PolyA signal"  
     misc_feature    7214..7859 
                     /label="ColE1"  
     misc_feature    5576..6370 
                     /label="Neo"  
     misc_feature    5207..5509 
                     /label="SV40 ori"  
     misc_feature    7874..8734 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3765..3771 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3765 
                     /label="OSBP2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGAAAGCG GCGGCTCCGA GCCGAGGCGG CGGCTGTGGC GGCCGCTCCC 
     1081 GCGGGCTCTC GTCGCTGTTC ACGGTTGTCC CCTGCCTGTC GTGCCACACG GCGGCGCCGG 
     1141 GCATGAGCGC TTCCACGTCC GGCTCCGGGC CGGAGCCCAA GCCCCAGCCC CAGCCCGTGC 
     1201 CCGAACCGGA GCGGGGACCG CTGTCAGAAC AGGTGTCGGA GGCAGTTTCG GAGGCAGTGC 
     1261 CAAGATCGGA ACCTGTGTCC GAGACGACGT CTGAGCCGGA GCCAGGGGCT GGGCAGCCAT 
     1321 CGGAACTGCT GCAGGGGTCG CGGCCGGGGT CAGAGTCAAG CTCAGGTGTA GGGGCTGGGC 
     1381 CCTTCACTAA GGCCGCATCG GAGCCGCTCT CCCGGGCGGT GGGGAGCGCG ACCTTTCTCA 
     1441 GACCCGAGTC AGGATCGCTG CCAGCGTTAA AGCCCCTGCC TCTTCTGCGA CCAGGACAGG 
     1501 CGAAGACTCC TCTTGGGGTT CCAATGTCGG GGACTGGCAC GACCTCCAGT GCCCCACTGG 
     1561 CCTTACTGCC TCTGGACAGC TTCGAGGGCT GGCTTCTCAA GTGGACCAAC TATCTGAAGG 
     1621 GCTACCAGCG CCGCTGGTTC GTGCTGGGCA ATGGTTTGCT CTCTTACTAC AGAAATCAGG 
     1681 GTGAAATGGC CCACACGTGC CGTGGAACCA TCAACCTGTC CACCGCGCAC ATTGACACGG 
     1741 AGGACTCTTG TGGTATCTTG CTGACCAGTG GGGCCAGGAG CTACCACCTC AAGGCCAGCT 
     1801 CAGAGGTGGA CCGGCAGCAG TGGATCACCG CCCTGGAGCT GGCCAAGGCC AAGGCTGTCC 
     1861 GCGTGATGAA CACTCATTCA GATGACTCTG GGGACGACGA CGAGGCTACC ACCCCAGCCG 
     1921 ACAAGAGCGA GCTGCACCAC ACCCTGAAGA ATCTTTCCCT GAAGTTAGAT GACCTCAGCA 
     1981 CGTGCAATGA CCTCATCGCC AAGCACGGCG CCGCACTCCA GCGCTCCCTG ACAGAGCTGG 
     2041 ACGGCCTCAA GATCCCATCT GAGAGTGGGG AGAAGCTGAA GGTGGTGAAT GAGCGGGCCA 
     2101 CCCTCTTCCG CATCACATCC AATGCTATGA TCAACGCCTG CAGGGACTTC TTGGAACTAG 
     2161 CAGAGATACA CAGTCGGAAA TGGCAGCGGG CACTGCAGTA TGAGCAGGAG CAGCGCGTGC 
     2221 ACTTGGAGGA AACCATTGAG CAGCTGGCGA AGCAGCACAA CAGCCTCGAG CGGGCCTTCC 
     2281 ACAGTGCCCC TGGCCGGCCG GCCAACCCCT CCAAGAGCTT CATTGAGGGA AGCCTCTTGA 
     2341 CTCCCAAAGG AGAGGACAGT GAGGAAGATG AAGATACCGA GTACTTTGAT GCCATGGAAG 
     2401 ACTCCACATC CTTCATCACC GTGATCACCG AGGCCAAGGA AGACAGAAAA GCTGAAGGTA 
     2461 GCACCGGGAC AAGTTCCGTG GACTGGAGCT CAGCAGACAA TGTACTAGAT GGTGCCTCGC 
     2521 TCGTGCCCAA GGGTTCATCC AAAGTCAAGA GGCGAGTCCG CATTCCCAAC AAGCCCAACT 
     2581 ACAGCCTTAA CCTCTGGAGC ATCATGAAGA ACTGCATCGG CCGGGAGCTC TCCAGGATCC 
     2641 CCATGCCGGT GAACTTCAAT GAGCCCCTGT CCATGCTCCA GCGGCTGACA GAGGACCTGG 
     2701 AGTACCACCA CCTGCTGGAC AAGGCAGTGC ACTGCACCAG CTCAGTGGAG CAGATGTGCC 
     2761 TGGTGGCCGC CTTCTCTGTG TCCTCCTACT CCACCACAGT GCACCGCATC GCCAAGCCCT 
     2821 TCAACCCCAT GCTGGGGGAG ACCTTCGAGC TGGACCGCCT CGACGACATG GGCCTGCGCT 
     2881 CCCTCTGTGA GCAGGTGAGC CACCACCCCC CCTCAGCTGC GCACTACGTG TTCTCCAAGC 
     2941 ATGGCTGGAG CCTCTGGCAG GAGATCACCA TCTCCAGCAA GTTCCGGGGA AAATACATCT 
     3001 CCATCATGCC GCTAGGTGCC ATCCACTTAG AATTCCAGGC CAGTGGGAAT CACTACGTGT 
     3061 GGAGGAAGAG CACCTCAACT GTTCACAACA TCATCGTGGG CAAGCTCTGG ATCGACCAGT 
     3121 CAGGGGACAT CGAGATTGTG AACCATAAGA CCAATGACCG GTGCCAGCTG AAGTTCCTGC 
     3181 CCTACAGCTA CTTCTCCAAA GAGGCAGCCC GGAAGGTGAC AGGAGTGGTG AGTGACAGCC 
     3241 AGGGCAAGGC CCATTACGTG CTGTCCGGCT CGTGGGATGA ACAAATGGAG TGCTCCAAGG 
     3301 TCATGCATAG CAGTCCCAGC AGCCCCAGCT CTGACGGGAA GCAGAAGACA GTGTACCAGA 
     3361 CCCTGTCAGC CAAGCTGCTG TGGAAGAAGT ACCCGCTGCC GGAGAACGCG GAGAACATGT 
     3421 ACTACTTCTC AGAGCTGGCC CTGACCCTCA ACGAGCACGA GGAGGGCGTA GCGCCAACCG 
     3481 ACAGCCGCCT GCGGCCCGAC CAGCGGCTGA TGGAGAAGGG CCGTTGGGAC GAGGCCAATA 
     3541 CCGAGAAGCA GCGGCTGGAG GAGAAGCAGC GCCTGTCGCG GCGCCGGCGG CTGGAGGCCT 
     3601 GCGGGCCGGG CAGCAGCTGC AGCTCGGAGG AAGAGAAGGA GGCGGATGCC TACACGCCAC 
     3661 TGTGGTTTGA GAAGAGGCTG GATCCGCTGA CCGGGGAGAT GGCCTGTGTG TACAAGGGCG 
     3721 GCTACTGGGA GGCCAAGGAG AAGCAAGACT GGCATATGTG CCCCAACATC TTCACGCGTA 
     3781 CGCGGCCGCT CGAGATGGAG AGCGACGAGA GCGGCCTGCC CGCCATGGAG ATCGAGTGCC 
     3841 GCATCACCGG CACCCTGAAC GGCGTGGAGT TCGAGCTGGT GGGCGGCGGA GAGGGCACCC 
     3901 CCGAGCAGGG CCGCATGACC AACAAGATGA AGAGCACCAA AGGCGCCCTG ACCTTCAGCC 
     3961 CCTACCTGCT GAGCCACGTG ATGGGCTACG GCTTCTACCA CTTCGGCACC TACCCCAGCG 
     4021 GCTACGAGAA CCCCTTCCTG CACGCCATCA ACAACGGCGG CTACACCAAC ACCCGCATCG 
     4081 AGAAGTACGA GGACGGCGGC GTGCTGCACG TGAGCTTCAG CTACCGCTAC GAGGCCGGCC 
     4141 GCGTGATCGG CGACTTCAAG GTGATGGGCA CCGGCTTCCC CGAGGACAGC GTGATCTTCA 
     4201 CCGACAAGAT CATCCGCAGC AACGCCACCG TGGAGCACCT GCACCCCATG GGCGATAACG 
     4261 ATCTGGATGG CAGCTTCACC CGCACCTTCA GCCTGCGCGA CGGCGGCTAC TACAGCTCCG 
     4321 TGGTGGACAG CCACATGCAC TTCAAGAGCG CCATCCACCC CAGCATCCTG CAGAACGGGG 
     4381 GCCCCATGTT CGCCTTCCGC CGCGTGGAGG AGGATCACAG CAACACCGAG CTGGGCATCG 
     4441 TGGAGTACCA GCACGCCTTC AAGACCCCGG ATGCAGATGC CGGTGAAGAA AGAGTTTAAA 
     4501 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4561 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4621 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4681 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4741 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4801 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4861 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4921 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4981 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5041 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5101 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5161 TTGGGTACGC GGCCAGCTTG GCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA 
     5221 GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT 
     5281 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA 
     5341 GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC 
     5401 CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCG 
     5461 GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA 
     5521 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG 
     5581 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG 
     5641 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG 
     5701 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT 
     5761 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA 
     5821 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG 
     5881 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT 
     5941 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA 
     6001 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG 
     6061 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG 
     6121 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG 
     6181 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC CGACCGCTAT 
     6241 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC 
     6301 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC 
     6361 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC 
     6421 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG 
     6481 GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT 
     6541 TCTTCGCCCA CCCCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA 
     6601 TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC 
     6661 TCATCAATGT ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT 
     6721 CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC 
     6781 GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA 
     6841 TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT 
     6901 GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC 
     6961 TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG 
     7021 CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG 
     7081 GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC 
     7141 GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG 
     7201 GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA 
     7261 CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC 
     7321 ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG 
     7381 TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT 
     7441 CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA 
     7501 GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA 
     7561 CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG 
     7621 TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTTTTTTT GTTTGCAAGC 
     7681 AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT 
     7741 CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA 
     7801 GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT 
     7861 ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA 
     7921 TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC 
     7981 GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG 
     8041 CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG 
     8101 CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT 
     8161 CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT 
     8221 CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT 
     8281 CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA 
     8341 AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA 
     8401 TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT 
     8461 AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC 
     8521 ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA 
     8581 GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT 
     8641 CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG 
     8701 CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT 
     8761 ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT 
     8821 AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC 
     8881 CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC 
     8941 TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG 
     9001 CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT 
     9061 TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC 
     9121 CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT 
     9181 TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA 
     9241 TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA 
     9301 ATTTT
//