LOCUS       RG223495                9122 bp    DNA     circular  SYN 
DEFINITION  DDX31 (tGFP-tagged) - Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 31 (DDX31), transcript variant 1
ACCESSION   NM_022779
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3601..4305 
                     /label="GFP"  
     misc_feature    4352..4937 
                     /label="PolyA signal"  
     misc_feature    7029..7674 
                     /label="ColE1"  
     misc_feature    5391..6185 
                     /label="Neo"  
     misc_feature    5022..5324 
                     /label="SV40 ori"  
     misc_feature    7689..8549 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3573..3580 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3573 
                     /label="DDX31"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGCCGGAT CTCGCTTCTC AGAGGCATTC AGAGAGCTTC CCCAGCGTGA 
     1081 ACTCTCGGCC GAATGTAATT TTGCCAGGCC GCGAGGGACG CAGAGAGGGG CTGCCACCAG 
     1141 GCGGGGGCAC GCGCGGTAGT CTTGTCCCTA CCCGCCCGGT GCCGCCGTCC CCGGCTCCCC 
     1201 TCGGGACGAG TCCGTACAGC TGGAGCCGTT CCGGACCCGG AAGGGGCGGT GGCGCAGGGT 
     1261 CCTCGCGCGT CCCACGTGGG GTCCCGGGCC CAGCAGTCTG CGCACCGGGC TCGCTGCTGC 
     1321 ACCACGCGTC ACCCACGCAG ACCATGGCAG CCGCCGACGG TTCGCTCTTC GACAACCCCA 
     1381 GGACGTTCTC CAGACGTCCC CCAGCCCAGG CGAGTCGGCA AGCAAAGGCT ACGAAAAGAA 
     1441 AATACCAAGC GTCCAGTGAG GCTCCCCCAG CGAAACGGAG GAACGAAACT TCATTTCTCC 
     1501 CAGCCAAGAA AACTAGTGTT AAAGAAACTC AGAGGACTTT TAAGGGGAAC GCACAAAAAA 
     1561 TGTTTTCTCC AAAGAAGCAT TCGGTTAGCA CAAGTGATAG AAACCAGGAG GAGAGACAGT 
     1621 GCATTAAGAC TTCATCACTG TTTAAAAACA ACCCTGACAT TCCAGAACTC CACAGACCTG 
     1681 TGGTAAAGCA GGTGCAAGAA AAAGTGTTTA CTTCAGCTGC TTTTCATGAG CTGGGCCTCC 
     1741 ACCCACATTT AATTTCCACA ATAAATACGG TCTTAAAAAT GTCTAGTATG ACCAGTGTTC 
     1801 AGAAGCAAAG TATTCCTGTG TTGCTGGAAG GCAGAGATGC TCTCGTGAGA TCCCAGACGG 
     1861 GCTCAGGTAA AACTCTTGCC TATTGCATCC CTGTGGTCCA GTCCCTTCAA GCAATGGAGT 
     1921 CAAAAATACA GCGCAGTGAT GGCCCCTATG CCCTGGTGCT CGTGCCAACG AGAGAGCTAG 
     1981 CTCTACAAAG CTTTGACACT GTCCAGAAAC TGCTTAAGCC TTTCACCTGG ATTGTGCCTG 
     2041 GAGTGTTAAT GGGAGGAGAG AAGAGAAAAT CAGAAAAGGC CAGACTCCGC AAAGGAATAA 
     2101 ATATCCTTAT CTCAACTCCT GGACGCCTGG TGGATCATAT AAAATCCACA AAGAACATTC 
     2161 ATTTTAGTCG GCTGCGGTGG TTGGTGTTTG ATGAAGCAGA CAGAATCTTG GATTTGGGTT 
     2221 TTGAAAAGGA CATCACAGTG ATACTTAATG CTGTAAATGC TGAATGCCAA AAACGACAGA 
     2281 ATGTCTTGCT ATCAGCGACA CTCACAGAAG GTGTAACGCG GCTAGCTGAT ATCAGTTTGC 
     2341 ATGATCCAGT CAGTATTTCT GTCCTGGACA AGAGCCATGA CCAGTTGAAC CCAAAGGACA 
     2401 AAGCGGTCCA GGAGGTCTGT CCTCCACCAG CTGGCGACAA GCTGGACAGC TTTGCAATAC 
     2461 CAGAGAGTCT CAAGCAGCAT GTGACTGTGG TTCCCAGCAA ACTGAGGCTT GTCTGCCTAG 
     2521 CGGCCTTCAT CCTTCAGAAA TGCAAGTTTG AGGAAGACCA GAAGATGGTT GTCTTTTTCT 
     2581 CAAGTTGCGA GCTGGTGGAG TTCCACTACA GCCTCTTCCT ACAGACCCTG CTGAGCAGCT 
     2641 CAGGGGCGCC GGCATCAGGG CAGTTGCCAT CTGCCTCCAT GCGATTAAAA TTCCTACGGC 
     2701 TGCATGGCGG CATGGAGCAG GAGGAAAGAA CAGCAGTGTT TCAGGAATTT TCACATTCCA 
     2761 GAAGAGGCGT CCTTCTTTGC ACGGATGTTG CAGCTCGGGG CTTAGATCTC CCTCAAGTCA 
     2821 CGTGGATTGT TCAGTACAAC GCTCCATCTT CACCTGCAGA ATACATCCAC CGGATTGGAA 
     2881 GAACCGCCCG GATTGGCTGC CATGGGAGCA GCCTGCTCAT TTTGGCTCCT TCGGAGGCAG 
     2941 AATATGTCAA CTCGTTGGCT TCTCACAAAA TCAACGTTTC TGAGATTAAG ATGGAAGATA 
     3001 TTTTGTGTGT TCTGACAAGA GATGATTGTT TTAAAGGGAA ACGATGGGGA GCCCAGAAAT 
     3061 CCCATGCTGT TGGCCCCCAG GAAATCCGAG AGCGAGCCAC AGTCTTGCAG ACGGTATTTG 
     3121 AAGATTACGT GCACTCCAGT GAGAGGAGGG TCTCCTGGGC AAAGAAAGCT CTGCAGTCCT 
     3181 TCATCCAAGC CTACGCCACC TACCCCAGGG AGCTGAAGCA CATCTTCCAC GTCCGATCCC 
     3241 TCCACCTTGG GCATGTGGCG AAGAGCTTCG GACTAAGAGA TGCCCCCAGG AATCTTAGTG 
     3301 CCTTGACTAG AAAGAAGAGG AAAGCACACG TGAAAAGGCC TGACCTTCAT AAGAAGACCC 
     3361 AGAGTAAACA CAGCCTCGCT GAAATCCTAC GTTCGGAATA CTCAAGCGGC ATGGAGGCCG 
     3421 ACATCGCCAA GGTCAAAAAG CAAAACGCAC CTGGAGAGCC TGGTGGCCGG CCCCTGCAGC 
     3481 ACAGTCTGCA GCCGACACCC TGCTTTGGCC GTGGGAAAAC ATTAAAATGG AGAAAAACCC 
     3541 AAAAAGGTGT ACAGCGGGAC AGCAAGACTT CCCAGAAAGT TAGCGGACCG ACGCGTACGC 
     3601 GGCCGCTCGA GATGGAGAGC GACGAGAGCG GCCTGCCCGC CATGGAGATC GAGTGCCGCA 
     3661 TCACCGGCAC CCTGAACGGC GTGGAGTTCG AGCTGGTGGG CGGCGGAGAG GGCACCCCCG 
     3721 AGCAGGGCCG CATGACCAAC AAGATGAAGA GCACCAAAGG CGCCCTGACC TTCAGCCCCT 
     3781 ACCTGCTGAG CCACGTGATG GGCTACGGCT TCTACCACTT CGGCACCTAC CCCAGCGGCT 
     3841 ACGAGAACCC CTTCCTGCAC GCCATCAACA ACGGCGGCTA CACCAACACC CGCATCGAGA 
     3901 AGTACGAGGA CGGCGGCGTG CTGCACGTGA GCTTCAGCTA CCGCTACGAG GCCGGCCGCG 
     3961 TGATCGGCGA CTTCAAGGTG ATGGGCACCG GCTTCCCCGA GGACAGCGTG ATCTTCACCG 
     4021 ACAAGATCAT CCGCAGCAAC GCCACCGTGG AGCACCTGCA CCCCATGGGC GATAACGATC 
     4081 TGGATGGCAG CTTCACCCGC ACCTTCAGCC TGCGCGACGG CGGCTACTAC AGCTCCGTGG 
     4141 TGGACAGCCA CATGCACTTC AAGAGCGCCA TCCACCCCAG CATCCTGCAG AACGGGGGCC 
     4201 CCATGTTCGC CTTCCGCCGC GTGGAGGAGG ATCACAGCAA CACCGAGCTG GGCATCGTGG 
     4261 AGTACCAGCA CGCCTTCAAG ACCCCGGATG CAGATGCCGG TGAAGAAAGA GTTTAAACGG 
     4321 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4381 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4441 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4501 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4561 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4621 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4681 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4741 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4801 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4861 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4921 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4981 GGTACGCGGC CAGCTTGGCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC 
     5041 TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA 
     5101 AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA 
     5161 ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT 
     5221 TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCGGCC 
     5281 TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG 
     5341 CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT 
     5401 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA 
     5461 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG 
     5521 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA 
     5581 CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT 
     5641 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG 
     5701 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA 
     5761 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT 
     5821 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC 
     5881 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC 
     5941 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA 
     6001 AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCCGA CCGCTATCAG 
     6061 GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC 
     6121 TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT 
     6181 CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGAAAT GACCGACCAA GCGACGCCCA 
     6241 ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA 
     6301 TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG CTGGAGTTCT 
     6361 TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA 
     6421 CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA 
     6481 TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG GCGTAATCAT 
     6541 GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG 
     6601 CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG 
     6661 CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA 
     6721 TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA 
     6781 CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG 
     6841 TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC 
     6901 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC 
     6961 CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC 
     7021 TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC 
     7081 TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA 
     7141 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC 
     7201 ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA 
     7261 ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG 
     7321 CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA 
     7381 GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG 
     7441 GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TTTTTTTGTT TGCAAGCAGC 
     7501 AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG 
     7561 ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA 
     7621 TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG 
     7681 AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT 
     7741 GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG 
     7801 AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC 
     7861 CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA 
     7921 CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC 
     7981 CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT 
     8041 CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC 
     8101 CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT 
     8161 TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC 
     8221 CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT 
     8281 GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA 
     8341 GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA 
     8401 TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG 
     8461 CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA 
     8521 AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT 
     8581 ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA 
     8641 AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT 
     8701 GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG 
     8761 CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG 
     8821 GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC 
     8881 GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT 
     8941 GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT 
     9001 TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT 
     9061 TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT 
     9121 TT
//