LOCUS       RG223371                8683 bp    DNA     circular  SYN 
DEFINITION  PEG10 (tGFP-tagged) - Human paternally expressed 10 (PEG10), transcript variant 1
ACCESSION   NM_015068
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3164..3868 
                     /label="GFP"  
     misc_feature    3915..4500 
                     /label="PolyA signal"  
     misc_feature    6592..7237 
                     /label="ColE1"  
     misc_feature    4954..5748 
                     /label="Neo"  
     misc_feature    4585..4887 
                     /label="SV40 ori"  
     misc_feature    7252..8112 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3143..3149 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3143 
                     /label="PEG10"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACCGAACGA AGAAGGGACG AGCTCTCTGA AGAGATCAAC AACTTAAGAG 
     1081 AGAAGGTCAT GAAGCAGTCG GAGGAGAACA ACAACCTGCA GAGCCAGGTG CAGAAGCTCA 
     1141 CAGAGGAGAA CACCACCCTT CGAGAGCAAG TGGAACCCAC CCCTGAGGAT GAGGATGATG 
     1201 ACATCGAGCT CCGCGGTGCT GCAGCAGCTG CTGCCCCACC CCCTCCAATA GAGGAAGAGT 
     1261 GCCCAGAAGA CCTCCCAGAG AAGTTCGATG GCAACCCAGA CATGCTGGCT CCTTTCATGG 
     1321 CCCAGTGCCA GATCTTCATG GAAAAGAGCA CCAGGGATTT CTCAGTTGAT CGTGTCCGTG 
     1381 TCTGCTTCGT GACAAGCATG ATGACCGGCC GTGCTGCCCG TTGGGCCTCA GCAAAGCTGG 
     1441 AGCGCTCCCA CTACCTGATG CACAACTACC CAGCTTTCAT GATGGAAATG AAGCATGTCT 
     1501 TTGAAGACCC TCAGAGGCGA GAGGTTGCCA AACGCAAGAT CAGACGCCTG CGCCAAGGCA 
     1561 TGGGGTCTGT CATCGACTAC TCCAATGCTT TCCAGATGAT TGCCCAGGAC CTGGATTGGA 
     1621 ACGAGCCTGC GCTGATTGAC CAGTACCACG AGGGCCTCAG CGACCACATT CAGGAGGAGC 
     1681 TCTCCCACCT CGAGGTCGCC AAGTCGCTGT CTGCTCTGAT TGGGCAGTGC ATTCACATTG 
     1741 AGAGAAGGCT GGCCAGGGCT GCTGCAGCTC GCAAGCCACG CTCGCCACCC CGGGCGCTGG 
     1801 TGTTGCCTCA CATTGCAAGC CACCACCAGG TAGATCCAAC CGAGCCGGTG GGAGGTGCCC 
     1861 GCATGCGCCT GACGCAGGAA GAAAAAGAAA GACGCAGAAA GCTGAACCTG TGCCTCTACT 
     1921 GTGGAACAGG AGGTCACTAC GCTGACAATT GTCCTGCCAA GGCCTCAAAG TCTTCGCCGG 
     1981 CGGGAAACTC CCCGGCCCCG CTGTAGAGGG ACCTTCAGCG ACCGGGCCAG AAATAATAAG 
     2041 GTCCCCACAA GATGATGCCT CATCTCCACA CTTGCAAGTG ATGCTCCAGA TTCATCTTCC 
     2101 GGGCAGACAC ACCCTGTTCG TCCGAGCCAT GATCGATTCT GGTGCTTCTG GCAACTTCAT 
     2161 TGATCACGAA TATGTTGCTC AAAATGGAAT TCCTCTAAGA ATCAAGGACT GGCCAATACT 
     2221 TGTGGAAGCA ATTGATGGGC GCCCCATAGC ATCGGGCCCA GTTGTCCACG AAACTCACGA 
     2281 CCTGATAGTT GACCTGGGAG ATCACCGAGA GGTGCTGTCA TTTGATGTGA CTCAGTCTCC 
     2341 ATTCTTCCCT GTCGTCCTAG GGGTTCGCTG GCTGAGCACA CATGATCCCA ATATCACATG 
     2401 GAGCACTCGA TCTATCGTCT TTGATTCTGA ATACTGCCGC TACCACTGCC GGATGTATTC 
     2461 TCCAATACCA CCATCGCTCC CACCACCAGC ACCACAACCG CCACTCTATT ATCCAGTAGA 
     2521 TGGATACAGA GTTTACCAAC CAGTGAGGTA TTACTATGTC CAGAATGTGT ACACTCCAGT 
     2581 AGATGAGCAC GTCTACCCAG ATCACCGCCT GGTTGACCCT CACATAGAAA TGATACCTGG 
     2641 AGCACACAGT ATTCCCAGTG GACATGTGTA TTCACTGTCC GAACCTGAAA TGGCAGCTCT 
     2701 TCGAGATTTT GTGGCAAGAA ATGTAAAAGA TGGGCTAATT ACTCCAACGA TTGCACCTAA 
     2761 TGGAGCCCAA GTTCTCCAGG TGAAGAGGGG GTGGAAACTG CAAGTTTCTT ATGATTGCCG 
     2821 AGCTCCAAAC AATTTTACTA TCCAGAATCA GTATCCTCGC CTATCTATTC CAAATTTAGA 
     2881 AGACCAAGCA CACCTGGCAA CGTACACTGA ATTCGTACCT CAAATACCTG GATACCAAAC 
     2941 ATACCCCACA TATGCCGCGT ACCCGACCTA CCCAGTAGGA TTCGCCTGGT ACCCAGTGGG 
     3001 ACGAGACGGA CAAGGAAGAT CACTATATGT ACCTGTGATG ATCACTTGGA ATCCACACTG 
     3061 GTACCGCCAG CCTCCGGTAC CACAGTACCC GCCGCCACAG CCGCCGCCTC CACCACCACC 
     3121 ACCGCCGCCG CCTCCATCTT ACAGTACCCT GACGCGTACG CGGCCGCTCG AGATGGAGAG 
     3181 CGACGAGAGC GGCCTGCCCG CCATGGAGAT CGAGTGCCGC ATCACCGGCA CCCTGAACGG 
     3241 CGTGGAGTTC GAGCTGGTGG GCGGCGGAGA GGGCACCCCC GAGCAGGGCC GCATGACCAA 
     3301 CAAGATGAAG AGCACCAAAG GCGCCCTGAC CTTCAGCCCC TACCTGCTGA GCCACGTGAT 
     3361 GGGCTACGGC TTCTACCACT TCGGCACCTA CCCCAGCGGC TACGAGAACC CCTTCCTGCA 
     3421 CGCCATCAAC AACGGCGGCT ACACCAACAC CCGCATCGAG AAGTACGAGG ACGGCGGCGT 
     3481 GCTGCACGTG AGCTTCAGCT ACCGCTACGA GGCCGGCCGC GTGATCGGCG ACTTCAAGGT 
     3541 GATGGGCACC GGCTTCCCCG AGGACAGCGT GATCTTCACC GACAAGATCA TCCGCAGCAA 
     3601 CGCCACCGTG GAGCACCTGC ACCCCATGGG CGATAACGAT CTGGATGGCA GCTTCACCCG 
     3661 CACCTTCAGC CTGCGCGACG GCGGCTACTA CAGCTCCGTG GTGGACAGCC ACATGCACTT 
     3721 CAAGAGCGCC ATCCACCCCA GCATCCTGCA GAACGGGGGC CCCATGTTCG CCTTCCGCCG 
     3781 CGTGGAGGAG GATCACAGCA ACACCGAGCT GGGCATCGTG GAGTACCAGC ACGCCTTCAA 
     3841 GACCCCGGAT GCAGATGCCG GTGAAGAAAG AGTTTAAACG GCCGGCCGCG GTCATAGCTG 
     3901 TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC 
     3961 TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT 
     4021 TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG 
     4081 GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG 
     4141 CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC 
     4201 CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT 
     4261 TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC 
     4321 AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC 
     4381 CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC 
     4441 ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC 
     4501 TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCTTGGC 
     4561 TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA 
     4621 TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG 
     4681 CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA 
     4741 CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC 
     4801 TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT 
     4861 AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT 
     4921 CCATTTTCGG ATCTGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG 
     4981 GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC 
     5041 AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG 
     5101 TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC 
     5161 GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG 
     5221 AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC 
     5281 ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC 
     5341 TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA 
     5401 CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG 
     5461 CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC CGACGGCGAG GATCTCGTCG 
     5521 TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT 
     5581 TCATCGACTG TGGCCGGCTG GGTGTGGCCG ACCGCTATCA GGACATAGCG TTGGCTACCC 
     5641 GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA 
     5701 TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG 
     5761 CGGGACTCTG GGGTTCGAAA TGACCGACCA AGCGACGCCC AACCTGCCAT CACGAGATTT 
     5821 CGATTCCACC GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG 
     5881 CTGGATGATC CTCCAGCGCG GGGATCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT 
     5941 TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC 
     6001 ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT 
     6061 CTGTATACCG TCGACCTCTA GCTAGAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT 
     6121 GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA 
     6181 AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC 
     6241 TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG 
     6301 AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT 
     6361 CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     6421 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     6481 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     6541 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     6601 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     6661 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     6721 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     6781 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     6841 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6901 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6961 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     7021 ACAAACCACC GCTGGTAGCG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     7081 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     7141 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     7201 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG 
     7261 TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT 
     7321 AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC 
     7381 CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA 
     7441 CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA 
     7501 GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA 
     7561 CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT 
     7621 CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC 
     7681 GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT 
     7741 CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC 
     7801 TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG 
     7861 CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT 
     7921 CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC 
     7981 CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG 
     8041 CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC 
     8101 ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG 
     8161 TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT 
     8221 TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC 
     8281 GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC 
     8341 TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT 
     8401 AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA 
     8461 ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC 
     8521 TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT 
     8581 CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG 
     8641 GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//